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Plant Cytogenetic Experts 

The workshop will involve two leading

experts from the Molecular

Cytogenetics Group at the University

of Leicester, UK, who have jointly

 published over 300 peer reviewed

articles in the field. This group

specializes in research to understandlarge-scale processes of genome

evolution, including chromosome

rearrangement, genome control,

recombinant, polyploidy, and

epigenetics. Much of their research is

focused on repetitive DNA, including

tandem repeated satellites, DNA

transposons and retrotransposons

involving technology development

work for in situ hybridization, photon

imaging and genomic data analysis. John Seymour (Pat) Heslop-Harrison 

Professor of Plant Cell

Biology and Molecular

Cytogenetics at the

University of Leicester,

UK. He is Chief Editor

of Annals of Botany, and

an accepting editor of

Theoretical and Applied

Genetics (TAG) and Plant Reproduction

(formerly Sexual Plant Reproduction). 

Trude Schwarzacher She is a co-project leader

of the Molecular

Cytogenetics research

group in the University

of Leicester. She is a

referee for many journals

including Plant Journal,

Chromosoma, Heredity,

Theoretical and Applied Genetics, PNAS,

Euphytica, Hereditas and Annals of Botany. 

REGISTRATION FORM

Name 

IC number 

Gender 

University/Institute/Company 

Email address 

Mailing address 

Phone number 

All participants are advised to read about FISH and

ChIP at webpages (http://www.molcyt.com and follow

links) before coming to the workshop. Please send registration form by email or fax to 

Fax: (603)7967 6991 

Email: cebar@um.edu.my Registration closing date: 20th November 2013 

FISH &  ChIP 

orkshop for plant

genetic study his unique workshop addresses the application

of florescence in situ hybridization (FISH) and

chromatin immunoprecipitation (ChIP) for

studies with plants. 

8/13/2019 Brochure event

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 FISH 

FISH (Fluorescence in situ hybridization) has

become an essential technique in molecular

cytogenetics, involving the combination of

molecular biology and cytogenetics. It can be

used to detect and localize the presences or

absence of specific DNA sequence on

chromosomes. FISH uses fluorescent probes

that bind to only those parts of the

chromosome with which they show a high

degree of sequence complementarity.

Fluorescence microscopy can be used to locate

where the fluorescent probe has bound to the

chromosomes. FISH requires a combination of

skills of molecular biology, cytogenetics,

microscopy, cellular imaging analysis and

immunocytochemistry. The workshop will

provide effective and efficient details of

protocols for FISH applied for plant

cytogenetics.

Co-organized by CEBAR University of Malaya, UMand Malaysia Agro-Biotechnology Institute, ABI 

 “FISH and ChIP”  This three day workshop will

include two main workshopsessions to cover use of each

technique for analysis of plants. 

ChIP 

ChIP  (Chromatin immunoprecipitation) allows

specific protein–DNA interactions to be isolated.

Combining ChIP with high-throughput sequencing

(ChIPseq) reveals the DNA sequence involved in

these interactions. The ChIP technique has been

successfully used in many biological systems to

study histone modifications of

eu/heterochromatin and localization of regulatory

chromatin factors. This method relies on the rapid

cross-linking of protein/DNA complexes within the

nucleus of living cells, followed by chromatinisolation, its random shearing and

immunoprecipitation with antibodies directed

towards proteins of interest. Levels of co-

immunoprecipitated DNA are analyzed by PCR.

The relative enrichment or depletion of a

particular DNA fragment in the

immunoprecipitated fraction reflects its in vivo

association with the examined protein. The

workshop will involve ChIP protocols for plant.

When 

17th December 2013 – 19th December 2013 Closing date for registration: 20th November 2013 

Where 

Malaysia Agro-biotechnology Institute, ABI

Serdang. (Blok A Administrative building) 

Registration fee RM 1300 for each participant(Includes tea and lunch each day) 

Workshops In depth educational sessions will be given byexperts covering FISH and ChIP techniques andapplications. All participants will get hands-onexperience directed by experts held with thepurpose of demonstrating the techniques orprocesses for both FISH and ChIP. Participants

work with and are exposed to equipment,specimens, chemicals and reagents. 

Lecture Series Participants will gain a global perspective on plantscience by attending the opening lecture on the17th  Dec 2013 morning session followed byspecialized lecture and discussion sessions,embedded within the workshop for participantsonly. 

Contact Us 

Centre for Research in Biotechnology for

Agriculture (CEBAR). Level 3, Institute of

Research Management & Monitoring (IPPP),

Research Management & Innovation

Complex, University of Malaya, 50603 Kuala

Lumpur. 

Tel: (6017)259 8211 

Fax: (603)7967 6991 

Email:cebar@um.edu.my 

Website: http://cebar.um.edu.my/