Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2-...
Transcript of Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2-...
![Page 1: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/1.jpg)
Biofilms
![Page 2: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/2.jpg)
Dental plaque
![Page 3: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/3.jpg)
Problems associated to biofilms
1- Physical effects: ships, tubing…
2- Metal surface corrosion
3- Development on medical implants
4- Associated diseases
![Page 4: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/4.jpg)
Tubing obstruction
Equipamiento dental >30años
![Page 5: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/5.jpg)
MS2-
M+
![Page 6: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/6.jpg)
Biofilm formation on a voice implant
![Page 7: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/7.jpg)
Contact lens
![Page 8: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/8.jpg)
Catheter
![Page 9: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/9.jpg)
P. Aeruginosa biofilm in lung
![Page 10: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/10.jpg)
![Page 11: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/11.jpg)
![Page 12: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/12.jpg)
Structure
![Page 13: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/13.jpg)
Structure
![Page 14: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/14.jpg)
![Page 15: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/15.jpg)
Biofilm formation process
![Page 16: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/16.jpg)
Glass surface in the lab
![Page 17: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/17.jpg)
![Page 18: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/18.jpg)
![Page 19: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/19.jpg)
MBF HBFLBFWT
Citrate
Glucose+Caa
Genetic analysis of biofilm formation
![Page 20: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/20.jpg)
Genetic characterization of biofilm formation
- Requirement of movement
- Adhesins
-Signaling
-Glycocalyx formation
![Page 21: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/21.jpg)
Movement requirement
-Flagelar-mediated mobility-Non-motile mutants in P. Fluorescens
-Twitching-Mutants in type IV pili in P. aeruginosa
-Suppression by cells “stickier”-ompR and adhesin Curli in E. coli
![Page 22: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/22.jpg)
Adhesins
-Type I pilus (fimH) in E. coli-Type IV (MSHA) pilus in V. cholerae-Curli adhesin
![Page 23: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/23.jpg)
Signaling
-Quorum sensing
![Page 24: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/24.jpg)
![Page 25: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/25.jpg)
QUORUM SENSING:DENSITY-DEPENDENT EXPRESSION
CULTURE DENSITY
AU
TO
IND
UC
ER
TRESHOLD
![Page 26: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/26.jpg)
GRAM-NEGATIVE COMMUNICATION:THE LuxI/LuxR LANGUAGE
LuxI
LuxR
LuxR
Target gene
![Page 27: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/27.jpg)
![Page 28: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/28.jpg)
GRAM-NEGATIVE COMMUNICATION:THE LuxI/LuxR LANGUAGE
-Light production in Vibrio fisheri-Virulence in Pseudomonas aeruginosa (LasI/R, RhlI/R)-Opine formation in Agrobacterium tumefaciens (TraR/I)
![Page 29: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/29.jpg)
GRAM-positive COMMUNICATION:THE PHEROMONE LANGUAGE
ABC
D
Target genePheromone gene
H
![Page 30: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/30.jpg)
HYBRID LANGUAGES: THE CASE OF Vibrio harvey
LuxO
Target gene
LuxN
LuxLM LuxS
LuxQ
![Page 31: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/31.jpg)
-Competence for DNA uptake in Bacillus subtilis and Streptoccocus pneumoniae (ComX/ComP/ComA)-Sporulation in Bacillus subtilis -Conjugation in Enteroccus fecalis-Virulence in Staphylococus areus
GRAM-positive COMMUNICATION:THE PHEROMONE LANGUAGE
![Page 32: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/32.jpg)
QS-mutants
![Page 33: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/33.jpg)
![Page 34: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/34.jpg)
Glycocalyx formation
-Alginate production in P. aeruginosa- EPS in V. cholerae (vps)
![Page 35: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/35.jpg)
![Page 36: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/36.jpg)
fimA
lasI
vps
flagella
rhl
algfimH
Curli
![Page 37: Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.](https://reader036.fdocuments.in/reader036/viewer/2022062515/56649f535503460f94c78118/html5/thumbnails/37.jpg)
Natural process of biofilm formation / Control by a blocking analogue