BD FACSAria™ Upgrades...who choose partial upgrades as well as those who choose full upgrades with...

For many users, the capability of an advanced cell sorter is defined by its flexibility, which in turn is defined by the number of parameters that can be detected simultaneously. With flexibility built-in, the BD FACSAria™ III system can support 6 lasers, 4 spatially separated beam spots, and up to 20 detector positions to measure up to 18 colors simulta neously. BD is constantly expanding the choice of lasers available for the BD FACSAria platform. Check the current technical specifications for the most up-to-date information.

Upgradeable and Backward Compatible The BD FACSAria sorter platform is designed to be upgradeable and backward compatible. That means you can design a configuration that meets your lab’s budget and site requirements today knowing that there will be a growth path as future needs dictate.

No other flow cytometer manufacturer offers this level of investment protection and commitment to its customers.

BD FACSAria™ Upgrades

2

The BD FACSAria upgrade program is one way BD keeps customers in a perpetual state of the art to protect BD technology investments. The upgrade program is specifically designed to renew older systems by retrofitting them to the current level of technology to make them more valuable to the laboratory. Even BD FACSAria systems purchased in 2003 can be upgraded.

Protect Your Technology Investment

Transform Your BD FACSAria Cytometer to the Latest TechnologyTechnology upgrades transform previous versions of the BD FACSAria system to the latest capabilities of the BD FACSAria III. A variety of upgrade scenarios are available to accommodate customers who choose partial upgrades as well as those who choose full upgrades with additional lasers. The upgrade from a BD FACSAria to a BD FACSAria III is performed in two stages. In the first stage the cytometer is upgraded to a BD FACSAria IIU. The second stage upgrades the BD FACSAria IIU cytometer to the capabilities of a BD FACSAria III. All upgrades are made in the field to minimize downtime.

Committed to Customer ServiceBD Biosciences is fully committed to the success and satisfaction of its customers. The BD FACSAria III upgrade program is backed by a world-class service and support organization with unmatched flow cytometry experience. Contact your sales representative to obtain more detailed information and to plan your BD FACSAria upgrade.

3

BD FACSAria to BD FACSAriaIIU

Fluidics Upgrade

Wetcart

Flow cell

Pinch valve

New fluidic tubing

Valves

Nozzles (70, 85, and

100 micron)

Laser shutter

BD FACSDiva v6.1

Complete Upgrade

Fluidics upgrade (above)

Sort block

Tube holders

Bulk injection aspirator

Splash shield

Enclosures

Options

Temperature control

Tube holders

1.5-mL, 12 x 75-mm,

and 15-mL holders

+

+

BD FACSAria

=

BD FACSAria IIU

BD FACSAria Upgrade Options Three options are available to upgrade the BD FACSAria:1. A fluidics-only upgrade that includes a new wetcart, flow cell,

pinch valve, new fluidic tubing, valves, and new nozzles (70, 85, and 100 µm). The 130-µm nozzle is optional. This upgrade also includes a new laser shutter and software upgrade to BD FACSDiva™ software v6.1.

2. A full upgrade to a BD FACSAria IIU that includes the fluidics upgrade above plus a new sort block, tube holders, bulk injection aspirator, splash shield, and associated enclosures.

3. A full upgrade to a BD FACSAria IIU with temperature control that includes the upgrades above plus new 1.5-mL Eppendorf-style, 12 x 75-mm, and 15-mL temperature control tube holders.

A BD FACSAria to BD FACSAria IIU upgrade is required as a first stage for customers who want to upgrade to a BD FACSAria III.

Upgrade Paths

4

BD FACSAria II or BD FACSAria IIU to BD FACSAria III

+ =561-nm or 445-nm Laser Upgrade

Optical X-mount

Next-generation cuvette

Fourth beam spot

Violet laser octagon for

systems with violet lasers

Options

Additional laser and PMTs

BD FACSAria IIBD FACSAria IIU

BD FACSAria III

BD FACSAria II and BD FACSAria IIU Upgrade OptionsAll BD FACSAria II and BD FACSAria IIU upgrades include the new optical X-mount, next-generation cuvette, and a fourth beam spot. For systems with a violet laser, a violet laser octagon is also included.

At the time of the upgrade, customers can choose to include additional lasers and PMTs. Special PMT kits are available to accommodate configuration requirements of a 561-nm or a 445-nm laser upgrade.

Fluidics Cart, Sheath Line Cuvette Flow Cell, Nozzle

Next-generation cuvette flow cell Cancer cell line (HT-29) of

Side Population Analysis

Fluidics cart Plenum reservoir tankCuvette flow cell

Ceramic nozzle

Fluidics System

Cuvette flow cell for BD FACSAria II

Closed-loop nozzle

Integrated nozzle

Fluidics cart

BD

FA

CSA

ria

BD

FA

CSA

ria

II B

D F

AC

SAri

a III

5

6

Optical system

Sort Block

Sort block

New sort block with new deflection plates

Collection Devices

Collection devices

Collection devices

Injection Chamber

Injection chamber

Laser Mount

Injection chamber

X-mount

Laser mount

7

BD FACSDiva Software

BD™ Cytometer Setup and Tracking (CS&T)

Electrical

Beam Spot

Fourth beam spot

Detector Configuration

Violet laser octagon

Outperforms analog from 0 to 100,000 events/sec

Proc

cess

ed E

vent

s

Event Rate

8000090000

70000600005000040000300002000010000

00 30000 18000060000 90000 120000 150000 210000

BD FACSAria III: uncompensatedBD FACSAria III: compensatedDead time of 5.5 �secBD FACSDiVa: compensated

Acquisition rates

8

BD FACSAria Family

Cat. No. Laser Expansion Kit

336833 BD FACSAria Violet Laser Field Upgrade for 2-Laser Base

336834 BD FACSAria Violet Laser Field Upgrade for 2-Laser Base on instrument with previous PMT upgrade

641190 2-Laser Near UV Upgrade Kit (for 2-Laser Configuration Only: Includes a Trigon)

641191 2-Laser Near UV Upgrade Kit (for 2-Laser Configuration on Instrument with Previous PMT Upgrade: Includes a Trigon)

641192 3-Laser Near UV Upgrade Kit (for 3-Laser Configuration Only)

Bandpass Filter Part No.

649422 Filter Set: Qdot® 705

649618 Filter Set: Alexa Fluor® 700







+ Detectable– Not detectable

3368

33

3368

34

6495

90

6495

91

6495

95

6411

90

6411

91

6411

92

6495

84

6495

85

6500

75

6500

76

6495

80

6500

77

6495

83

6494

22

6496

18

3437

95

3437

96

3437

93

3438

00

6494

22

3437

88

Laser Fluorochrome Bandpass Filter

488 nm FITC 530/30

PE 585/42 – – –

BD Horizon™ PE-CF594 616/23 – – –

PE-Texas Red 616/23 – – –

PerCP 675/20

PerCP-Cy™5.5 695/40

PE-Cy™7 760/60 – – –

633 nm APC 660/20

Alexa Fluor® 700 730/45 +

APC-H7 780/60

405 nm BD Horizon™ V450 450/50 + + + + + +

BD Horizon™ V500510/50 or

530/30+ + + + + +

Qdot® 565 560/40 +

Qdot® 585 585/42 +

Qdot® 605 610/20 +

Qdot® 655 670/30 +

Qdot® 700 710/50 +

Qdot® 800 800/30 +

561 nm PE 585/42 + + +

BD Horizon PE-CF594 610/20 + + +

PE-Texas Red 610/20 + + +

PE-Cy™5 670/14 + + +

PE-Cy5.5 710/50 +

PE-Cy7 780/60 + + +

445 nm CFP 510/80 + +

375 nm Hoechst Blue 450/20 + + + +

Hoechst Red 670 LP + + + +

405-nm and 375-nm Laser Upgrades for Systems Without a 561-nm or a 445-nm Laser

Upgrade Kit

9

Add 3rd Laser Add 4th Laser Add 5th Laser Add 6th Laser

445 nm 650077 375 nm 649584 405 nm 649595 561 nm 649580

561 nm 650075 405 nm 649590 561 nm 649580 405 nm 649595

561 nm 649580 375 nm 641192 561 nm 649580

375 nm 649584 561 nm 649580 375 nm 641192

405 nm 649590 375 nm 649584 405 nm 649595

445 nm 649583 405 nm 649590 375 nm 641192

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

375 nm 641192 445 nm 649583

445 nm 649583 375 nm 641192

375 nm 649584 405 nm 649595

405 nm 649590 375 nm 641192

445 nm 650077 375 nm 649595 561 nm 649580

561 nm 650076 561 nm 649580 405 nm 649595

375 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

445 nm 650077 405 nm 649595 561 nm 649580

561 nm 650076 561 nm 649580 405 nm 649595

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

445 nm 650077 561 nm 650076

561 nm 650076 445 nm 649583

UPgrADE OPTIONS gUIDE

Upgrade Kit Part No. Laser

DetectorsMirror Filter X-Mount

Next-generation Flow Cell

4th Beam Spot Laser SelectorOctagon Trigon PMT

336833 405 nm 1 2 502 LP 450/40 BP, 530/30 BP

336834 405 nm 1 2 502 LP 450/40 BP, 530/30 BP

641190 375 nm 1 2 610 LP 450/20 BP, 670 LP

641191 375 nm 1 2 610 LP 450/20 BP, 670 LP

641192 375 nm 610 LP 450/20 BP, 670 LP

649590 405 nm 1 2 502 LP 450/40 BP, 510/50 BP

649591 405 nm 1 502 LP 450/40 BP, 510/50 BP

649595 405 nm 502 LP 450/40 BP, 510/50 BP

649584 375 nm 1 2 610 LP 450/20 BP, 670 LP

649585 375 nm 1 610 LP 450/20 BP, 670 LP

650075 561 nm 1 1600 LP 630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

650076 561 nm 1600 LP 630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

649580 561 nm 1 1600 LP630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

650077 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

649583 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

Kit Contents

BD FACSAria Two-Laser System (488 nm/633 nm)

BD FACSAria Four-Laser System (488 nm/633 nm/405 nm/375 nm)

BD FACSAria Three-Laser System (488 nm/633 nm/375 nm)

BD FACSAria Three-Laser System (488 nm/633 nm/405 nm)

10

BD FACSAria IIU


3368

33

3368

34

6495

91

6495

95

6495

95

6479

62

6479

63

6506

44

6506

45

6495

76

6496

02

6495

80

6496

04

6495

83

6494

22

6496

18

3437

95

3437

96

3437

93

3438

00

6494

22

3437

88

6507

51


488 nm FITC 530/30

PE 585/42 – – –

BD Horizon PE-CF594 616/23 – – –


PerCP 675/20

PerCP-Cy5.5 695/40

PE-Cy7 760/60 – – –

633 nm APC 660/20


APC-H7 780/60

405 nm BD Horizon V450 450/50 + + + + +

BD Horizon V500510/50 or

530/30

+ + + + +

Qdot® 565 560/40 +

Qdot® 585 585/42 +

Qdot® 605 610/20 +

Qdot® 655 670/30 +

Qdot® 705 710/50 +

Qdot® 800 800/30 + +

561 nm PE 585/42 + + +



PE-Cy5 670/14 + + +

PE-Cy5.5 710/50 +

PE-Cy7 780/60 + + +

445 nm CFP 510/80 + +

375 nm Hoechst Blue 450/20 + + + +

Hoechst Red 670 LP + + + +












336833 BD FACSAria Violet Laser Field Upgrade for 2-Laser Base

336834 BD FACSAria Violet Laser Field Upgrade for 2-Laser Base on Instrument with Previous PMT Upgrade

647961 2-Laser Base Near UV Upgrade Kit

647962 2-Laser Base on Instrument with Previous PMT Upgrade, Near UV Upgrade Kit

647963 3-Laser Near UV Upgrade Kit

Upgrade Kit

11


445 nm 649604 375 nm 650644 405 nm 649595 561 nm 649580

561 nm 649578 405 nm 649590 561 nm 649580 405 nm 649595

561 nm 649580 375 nm 647963 561 nm 649580

375 nm 650644 561 nm 649580 375 nm 647963

405 nm 649590 375 nm 650644 405 nm 649595

445 nm 649583 405 nm 649590 375 nm 647963

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

375 nm 647963 445 nm 649583

445 nm 649583 375 nm 647963

375 nm 650644 405 nm 649595

405 nm 649590 375 nm 647963

445 nm 649604 375 nm 650645 561 nm 649580

561 nm 649602 561 nm 649602 405 nm 647963

375 nm 647963 445 nm 649583

445 nm 649583 405 nm 647963

445 nm 649604 405 nm 649591 561 nm 649580

561 nm 649602 561 nm 649602 405 nm 649595

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

445 nm 649604 561 nm 649602

561 nm 649602 445 nm 649583


Kit Contents

BD FACSAria IIU Four-Laser System (488 nm/633 nm/405 nm/375 nm)

BD FACSAria IIU Three-Laser System (488 nm/633 nm/375 nm)

BD FACSAria IIU Three-Laser System (488 nm/633 nm/405 nm)

BD FACSAria IIU Two-Laser System (488 nm/633 nm)





336833 405 nm 1 2 502 LP 450/40 BP, 530/30 BP

336834 405 nm 1 2 502 LP 450/40 BP, 530/30 BP

647962 375 nm 1 2 610 LP 450/20 BP, 670 LP

647963 375 nm 610 LP 450/20 BP, 670 LP

649590 405 nm 1 2 502 LP 450/40 BP, 510/50 BP

649591 405 nm 1 502 LP 450/40 BP, 510/50 BP

649595 405 nm 502 LP 450/40 BP, 510/50 BP

650644 375 nm 1 2 610 LP 450/20 BP, 670 LP

650645 375 nm 1 610 LP 450/20 BP, 670 LP

649576 561 nm 1 1600 LP630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

649602 561 nm 1 1600 LP630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

649580 561 nm 1 1600 LP630 LP735 LP

582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

649604 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

649583 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

12

BD FACSAria II


6431

56

6437

55

6437

56

6495

92

6495

93

6495

95

6437

78

6437

79

6495

86

6495

89

6495

87

6495

78

6496

03

6495

80

6496

05

6495

83

6507

46

6507

45

6507

47

6507

48

6507

49

6507

50

6507

51


488 nm FITC 530/30

PE 585/42 – – –

BD Horizon PE-CF594 616/23 – – –


PerCP 675/20

PerCP-Cy5.5 695/40

PE-Cy7 760/60

633 nm APC 660/20


APC-H7 780/60

405 nm BD Horizon V450 450/50 + + + + + +

BD Horizon V500510/50 or

530/30

+ + + + + +

Qdot® 565 560/40 +

Qdot® 585 585/42

Qdot® 605 610/20 +

Qdot® 655 670/30 +

Qdot® 705 710/50 +

Qdot® 800 800/30 +

561 nm PE 585/42 + + +



PE-Cy5 670/14 + + +

PE-Cy5.5 710/50 +

PE-Cy7 780/60 + + +

445 nm CFP 510/80 + +

375 nm Hoechst Blue 450/20 + + + + +

Hoechst Red 670 LP + + + + +



643156 BD FACSAria II Violet Laser Field Upgrade for 2-Laser Base

643755 BD FACSAria II Violet Laser Field Upgrade for 2-Laser Base on Instrument with Previous PMT Upgrade

643756 BD FACSAria II Violet Laser Field Upgrade for 3-Laser System (Blue/Red/Near UV)

643778 BD FACSAria II Near UV (375 nm) Upgrade Kit for 2-Laser Base on Instrument with Previous PMT Upgrade

643779 BD FACSAria II Near UV (375 nm) Upgrade Kit for 3-Laser System

PMT Upgrade Kits*

650743 Channel DAQ & SCI Upgrade Kit

650746 PE-Cy5.5 PMT

650745 Alexa Fluor® 700

650747 Qdot® 565

650748 Qdot® 605

650749 Qdot® 655

650750 Qdot® 705

650751 Qdot® 800










* PMT Upgrade Kits include the appropriate filter sets.

Upgrade Kit

13


445 nm 649605 375 nm 649586 405 nm 649595 561 nm 649580

561 nm 649578 405 nm 649592 561 nm 649580 405 nm 649595

561 nm 649580 375 nm 649589 561 nm 649580

375 nm 649586 561 nm 649580 375 nm 649589

405 nm 649592 375 nm 649586 405 nm 649595

445 nm 649583 405 nm 649592 375 nm 649589

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

375 nm 649589 445 nm 649583

445 nm 649583 375 nm 649589

375 nm 649586 405 nm 649595

405 nm 649592 375 nm 649589

445 nm 649605 375 nm 649587 561 nm 649580

561 nm 649603 561 nm 649603 405 nm 649589

375 nm 649589 445 nm 649583

445 nm 649583 405 nm 649589

445 nm 649605 405 nm 649593 561 nm 649580

561 nm 649603 561 nm 649603 405 nm 649595

405 nm 649595 445 nm 649583

445 nm 649583 405 nm 649595

445 nm 649605 561 nm 649603

561 nm 649603 445 nm 649583






643156 405 nm 1 2 502 LP 530/30 BP, 530/30 BP

643755 405 nm 1 2 502 LP 450/40 BP, 530/30 BP

643756 405 nm 610 LP 450/40 BP, 530/30 BP

643778 375 nm 1 2 610 LP 450/20 BP, 670 LP

643779 375 nm 502 LP 450/20 BP, 670 LP

649592 405 nm 1 2 502 LP 450/40 BP, 510/50 BP

649593 405 nm 1 502 LP 450/40 BP, 510/50 BP

649595 405 nm 610 LP 450/40 BP, 510/50 BP

649586 375 nm 1 2 610 LP 450/20 BP, 670 LP

649589 375 nm 600 LP 450/20 BP, 670 LP

649578 561 nm 1 1630 LP735 LP

582/15 BP, 610/20 BP,670/14 BP, 780/60 BP

649603 561 nm 1 1600 LP630 LP735 LP

582/15 BP, 610/20 BP,670/14 BP, 780/60 BP

649580 561 nm 1 1600 LP 630 LP 735 LP

582/15 BP, 610/20 BP,670/14 BP, 780/60 BP

649605 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

649583 445 nm 470 LP510/80 BP, 445/15 BP,480/20 BP

Kit Contents

BD FACSAria II Four-Laser System (488 nm/633 nm/405 nm/375 nm)

BD FACSAria II Three-Laser System (488 nm/633 nm/375 nm)

BD FACSAria II Three-Laser System (488 nm/633 nm/405 nm)

BD FACSAria II Two-Laser System (488 nm/633 nm)

14

PMT Upgrade Kits*

650743 CHANNEL DAQ & SCI Upgrade Kit

650746 PE-Cy5.5 PMT

650745 Alexa Fluor® 700

650747 Qdot® 565

650748 Qdot® 605

650749 Qdot® 655

650750 Qdot® 705

650751 Qdot® 800










Cat. No. Laser Upgrades

649595 405 nm for BD FACSAria III with a 375-nm Laser

649592 405 nm for BD FACSAria III without a 375-nm Laser

649589 375 nm for BD FACSAria III with a Violet Laser

649586 375 nm for BD FACSAria III without a Violet Laser

649580 561 nm Laser Upgrade

649583 445 nm Laser Upgrade

+ Detectable– Not detectable 64

9595

6495

92

6495

86

6495

89

6495

80

6495

83

6507

46

6507

45

6507

47

6507

48

6507

49

6507

50

6507

51


488 nm FITC 530/30

PE 585/42 –

BD Horizon PE-CF594 616/23 –

PE-Texas Red 616/23 –

PerCP 675/20

PerCP-Cy5.5 695/40

PE-Cy7 760/60 –

633 nm APC 660/20


APC-H7 780/60

405 nm BD Horizon V450 450/50 + +

BD Horizon V500 510/50 + +

Qdot® 565 560/40 +

Qdot® 585 585/42

Qdot® 605 610/20 +

Qdot® 655 670/30 +

Qdot® 700 710/50 +

Qdot® 800 800/30 +

561 nm PE 585/42 +

BD Horizon PE-CF594 610/20 +

PE-Texas Red 610/20 +

PE-Cy5 670/14 +

PE-Cy5.5 710/50 +

PE-Cy7 780/60 +

445 nm CFP 510/80 +

375 nm Hoechst Blue 450/20 + +

Hoechst Red 670 LP + +

BD FACSAria III UPgrADE OPTIONS gUIDE

BD FACSAria III Laser and PMT Upgrades

Kit Contents


DetectorsMirror FilterOctagon Trigon PMT

649595 405 nm 502 LP 450/40 BP, 510/50 BP

649592 405 nm 1 2 502 LP 450/40 BP, 510/50 BP

649589 375 nm 610 LP 450/20 BP, 670 LP

649586 375 nm 1 2 610 LP 450/20 BP, 670 LP

649580 561 nm 1 1 600 LP, 630 LP, 735 LP 582/15 BP, 610/20 BP, 670/14 BP, 780/60 BP

649583 445 nm 470 LP 510/80 BP, 445/15 BP, 480/20 BP

Upgrade Kit

* PMT Upgrade Kits include the appropriate filter sets.

15

BD FACSAria Family FLUIDICS SYSTEM UPgrADE

Information About the Fluidics Upgrade to BD FACSAria IIU

Configuration 643895 643985 643986

BD FACSDiva Software

New Fluidics Cart

Updated Fluidics

Cuvette Flow Cell

Integrated Ceramic Nozzles

Sort Block Upgrade

Updated Collection Devices

Temperature Control Tube Holders

Updated Injection Chamber

Laser Shutter

Protective Cover Flow Cell

Cat. No. Fluidics Upgrade

643895 Fluidics Upgrade Kit for BD FACSAria

643985 Upgrade: Full Conversion from BD FACSAria to BD FACSAria II

643986 Upgrade: Full Conversion from BD FACSAria with Temperature Control to BD FACSAria II

23-13449-01

BD Biosciences 2350 Qume Drive San Jose, CA 95131 bdbiosciences.com

© 2011 Becton, Dickinson and Company. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in retrieval systems, or translated into any language or computer language, in any form or by any means electronic, mechanical, magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD Biosciences.

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited. Product availability and prices are subject to change without notice.

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BD FACSAria™ Upgrades...who choose partial upgrades as well as those who choose full upgrades with...

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