Analysis and Sorting of T reg Cells on the FACSAria
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Analysis and Sorting of T reg Cells on the FACSAria. John Daley Director of Flow Cytometry Hematologic Neoplasia Dana-Farber Cancer Institute Harvard Medical School. Picture credit:. http://www.bdbiosciences.com/features/products. From BD Biosciences Website Featured products: - PowerPoint PPT Presentation
Transcript of Analysis and Sorting of T reg Cells on the FACSAria
Analysis and Sorting of T reg Cells on the FACSAriaJohn DaleyDirector of Flow CytometryHematologic NeoplasiaDana-Farber Cancer InstituteHarvard Medical Schoolhttp://www.bdbiosciences.com/features/productsPicture credit:
http://www.bdbiosciences.com/features/products/display_product.php?keyID=162From BD Biosciences WebsiteFeatured products:Regulatory T cells
The way we used to sort Human CD4+CD25+ PBLs on the FACSAriaSORT TOP2- 3% CD25 HI
Then CD45Ra was added to the Sort Design
Multicolor(5) Minor populations : Four way sorting: Treg StoryCD45ra FITCCD127 PECD25 PE-CY5CD4 PE-CY7CD3 PAC BLUE
Compensation ComparisonsManual Comp with cells and actual reagentsAutocomp with cells and actual reagentsAutocomp with beads and actual reagentsAutocomp with beads and CD45+ reagentsAutocomp with cells and CD45+ reagents
BD cells Cell AutocompBD cellsBead AutoComp
Treg Post Sort Reanalysis
99.497.198.4
99.9POST SORT REANALYSIS: HUMAN PBL CD3+CD4+ CD45RA+ &CD45RA- Treg+/Teffector+/ --
Actual reagents cells compensationcellsbeadsActual reagents beads compensationcellsbeads
FoxP3 Expression in CD4+ Treg CD45RA+
Fluorescence minus 1
