AP Lab 7Wisconsin Fast Plants“Who’s The Father?

What are Wisconsin Fast Plants?Read the lab and do p.2

• Type of mustard, radish, cabbage plant especially bred by Paul Williams of the University of Wisconsin

WFP Life Cycle Video

• Fast Plants Time Lapse Video

See stems and ANL (purple) and anl (non-purple or green)

Grow seeds in Petri dishUse Lid as bottom

Other part as cover Cut paper towel circle• Wet paper towel well with

a pipette

NAME

Water is to break the seed coat

Germinates in a day or two

FRIDAY

-Mark Petri dish lid into two halves

-Label P on one side, F1 the other

-Moisten paper towel circle and place 5 seeds in each side of the lid

F2 WFP (1:57)

P F1Put you initials on the Petri dish cover

Root emerges with root hairs to increase surface area for water absorption.

Petri dish at slight angle under a light

It will sit all week-end so be sure there is enough water in the Petri dish.

Day4 (MONDAY)(cont. on next slide)

• Stem and leaves emerge• Water F1 and P well.

• “Observing the Seeds” (make 3 quantitative and 3 qualitative observations, sketch and color, count the genotypes and phenotypes.)

• Attach a paper with these observations.

Monday: Now plant F2

• Place 10 seeds in a Petri dish• Two rows of 5• Water well.• Label F2

• Cover• Label your initials• Put under light at

a slight angle

0 0

0 0

0 0

0 0

0 0

Tuesday

• Take data for F1 and P

• Check on seeds –add water if needed

Day 7 (Friday)

• DATA COLLECTION DAY OF F2

Purple/StandardNon-Purple/StandardPurple/Rosette-DwarfNon-Purple/ Rosette Dwarf

(see pictures next slide)

Friday: Count F2

Rosette dwarf

Purple/standard stemNon-purple/Standard stem

Collect Group Data

• P. 6• Sketch plants, color• Do dihybrid of the plant genotypes

Chi square Test

• Bozeman Biology Chi Square Test• To test your null hypothesis• Critical value and degrees of freedom

Day14-20 (flowers)

How do you grow WFP?

•Need constant light

How do you grow WFP?

•Seeds are tiny-do not cover with very much potting soil

How do you grow WFP?

•Need fertilizer

Label your quads

Green and Non-green leaves

• Wisconsin Fast Plants® F1 Non-Purple Stem, Yellow-Green Leaf (F1 Anthocyaninless, Yellow-Green)

• F1 seed from cross of Non-Purple Stem, Yellow-Green Leaf (anl/ygr) × Purple Stem (ANL/YGR);