226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour:...
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Transcript of 226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour:...
226 PHT Final Spots
Gram’s stain
Results:
Shape: Shape: CocciCocci
Arrangment: Arrangment: irregularirregular clustersclusters
Colour: Colour: VioletViolet
Gram’s reaction: Gram’s reaction: Gram’sGram’s +ve+ve
Name of microorganism: Name of microorganism:
StaphylococciStaphylococci
Results:
Shape: Shape: OvalOval
Arrangment: Arrangment: SingleSingle
Colour: Colour: VioletViolet
Gram’s reaction: Gram’s reaction: Gram’s +veGram’s +ve
Name of microorganism: Name of microorganism:
Candida Candida
Results:
Shape: Shape: BacilliBacilli
Arrangment: Arrangment: ChainsChains
Colour: Colour: VioletViolet
Gram’s reaction: Gram’s reaction: Gram’s +veGram’s +ve
Name of microorganism: Name of microorganism:
Bacillus Bacillus
Results:
Shape: Shape: RodsRods
Arrangment: Arrangment: SingleSingle
Colour: Colour: redred
Gram’s reaction: Gram’s reaction: Gram’s –veGram’s –ve
Name of microorganism: Name of microorganism:
Gram negative Gram negative bacillibacilli
Negative stainingNegative staining
Candida
Negative staining
Staphylococci
Negative staining of Bacillus
Results
Type of Staining: Acid fast stain
Shape: beaded bacilli
Arrangement: Tree shaped
Colour: red
Name of microorganism: M.phelei
Spore Stain ofBacillus subtilis
Type of Staining: Spore stain
Shape: bacilli
Arrangement: Chains
Colour of spores: green
Colour of vegetative cells: red
Name of microorganism:
B. subtilis
Nutrient agar plateNutrient agar plate Nutrient agar slantNutrient agar slant
Nutrient brothNutrient broth
Simple media
Enriched media
Serum agar blood agar
non-hemolytic Sterptococci.
β-hemolytic Sterptococci.α-hemolytic Sterptococci.
Hemolysis of blood agar
Selective medium
L-J
Selective medium
Cetremide agar of pseudomonas
Growth with yellow colour around the colonies S.aureus
Growth without change in the colour of the medium S.epidermidis
1-Mannitol salt agar1-Mannitol salt agar
Selective and differential media:Selective and differential media:
Lactose fermenter Pink colonies
Lactose non-fermenter Pale colonies
2-MacConkey agar2-MacConkey agar
Cooked meat
Anaerobic Jar
Results:Positive test: formation of visible clot.
Coagulase +ve
S.aureus
Coagulase -ve
S.epidermidis
1- Coagulase Test:1- Coagulase Test:
2-Oxidase test:
Results:+ve Test: Appearance of purple colour within 1-2 min.
purple colour
+ve test
No colour
-ve test
Results:
+ve
-ve
3- Urease Test:3- Urease Test:
1. Sugar fermentation:1. Sugar fermentation:
Glucose Maltose Sucrose Glucose Maltose Sucrose
Sugar fermentation can be indicated by change of color of the medium from red to yellow.
+ve - ve+ve +ve +ve +ve
Results:
Butt:
Slant:
H2S Production:
acidic (yellow)
acidic (yellow)
-ve
acidic (yellow)
alkaline (red)
-ve
acidic (yellow)
alkaline (red)
+ve
2- 2- Triple Sugar Iron (TSI) agar:Triple Sugar Iron (TSI) agar:
O-/F- O+/F+ O+/F- O-/F+
Results:
Fermentative OxidativeNon Saccharolytic
Positive Test:
3-O/F Test (Oxidation Fermentation Test)
Positive Test:
4-Sensitive Oxidation Fermentation (O/F)Test:
O-/F- O+/F+ O+/F- O-/F+
Results:
Fermentative OxidativeNon Saccharolytic
Results:
+ve-ve
1- Indole test
Methods for determination of MIC
E-test:-
Elek’s Toxigenicity Test: