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Thermo Scientific Dionex UltiMate 3000 Diode Array and Multiple-Wavelength Detectors Fast, sensitive, and rugged UV-Vis detection

Integrating hardware, software and separation chemistry, Thermo Fisher Scientific offers UHPLC to everyone – for all needs – establishing the new standard in conventional LC.

The Thermo Scientific™ Dionex™ UltiMate™ DAD 3000 detector is a high-resolution, 1024-element diode array detector (DAD) available in Rapid Separation (200 Hz) and Standard (100 Hz) versions. It operates with the Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System software to provide a variety of spectra views, including 3-D plotting and automated chromatogram handling. The high resolution and low-noise performance of the DAD-3000 family makes it ideal for the most sensitive and accurate library searches and peak purity analyses.

The detector is also available as a multiple wavelength detector (MWD) in Standard (100 Hz) and Rapid Separation (200 Hz) versions.

• Datacollectionatupto200Hzusingamaximumofeightsingle-wavelengthdatachannelsandone3-Dfield(3-DonlywithDAD-3000(RS))forbestsupportofultrafastseparations

• Standardversionsoperateatupto100Hzdatacollectionrateforoptimumsupportof62MPa(9000psi)UltiMate3000Standardsystems

• Accuratecompoundconfirmationwitha1024-element,highresolutionphotodiodearray

• FlexibilityinbothUVandVisapplicationswith190–800nmwavelengthrange

• Low-noiseoverthefullspectralrangeusingdeuteriumandtungstenlamps

• Fastandaccuratewavelengthverificationusingabuilt-inholmiumoxidefilter

• ThedetectorcanbeupgradedwiththeThermoScientific™Dionex™UltiMate™3000pHandConductivityMonitor(PCM-3000)foraccuratemonitoringpHgradients

• Excellentreliabilityandreproducibilitywithlowbaselinedrift(typically<500µAU/h)

• Simplifiedroutinemaintenancewithfrontaccesstopre-alignedcellsandlamps

• IDchipsonflowcellsandlampsforidentificationandlife-spanmonitoring

• ChromeleonCDSsoftwareforfullcontrolandflexibledatahandling

• Front-paneldisplayforeasymonitoringofdetectorstatustomaximizeuptime

• Flowcellsforsemi-micro,semi-analytical,analytical,andsemi-preparativeapplications

• Flowcellsavailableinstainlesssteelandbiocompatibleversions

2 Table1.DAD-3000/MWD-3000designfeaturesandhighlights

DAD-3000/MWD-3000 Series Design Features and Highlights Their Benefit to You

Deuteriumandtungstenlampssupportawidedetectionwavelengthrangefrom190nmto800nm.

Goodsignal-to-noiseratioacrossthewavelengthrange.Highapplicationflexibility.

A1024-elementphotodiodearrayoptimizesspectralresolution. Accuratecompoundconfirmation.

Uptoeightchannelsofsingle-wavelengthdatacollection.Simultaneously,theDADcanacquire3-Ddata.

Simultaneousquantitationunderindividually-optimizedconditions.

Upto200Hzdatacollectionrate,including3-Ddata(underChromeleon7.1softwarecontrol).

Bestchromatographicseparationefficiencyevenwithultrafastseparations.

Excellentlinearity,typicallyupto2.0AU.Combinesdrugassayandimpurityprofilinginpharmaceuticalanalysiswithnoneedtoreinjectatadifferentdilution.

VariableslitforRapidSeparationversionsoftheDADandtheMWD.Achievebestspectralresolutionwiththenarrowslitsetting,improvenoiseperformancewiththewideslitsetting.

Flowcellrangecoverssemi-micro,semi-analytical,analytical,andsemi-preparativeflowrateapplications,instainlesssteelandbiocompatibleversions.

Flowcelleasilyadaptabletothecolumnformatinuse.

UpgradeanMWDtoaDADfor3-DUVfieldacquisitionsupport.Splityourinvestmentindiodearraytechnology.Purchasetheinstrumentyouneedtodayandadaptittoyourfutureapplicationneedslater.

Fastandtool-freeexchangeoflampsandflowcellsfromthedetectorfront.

Maintenancetasksareeasytodo.

LampsandflowcellsequippedwithIDchipsforidentification(e.g.,typeandserialnumber)andlifespanmonitoring.

HistoryistraceableevenwhentheflowcellorlampisplacedinanotherDAD-3000orMWD-3000seriesmodule.

Chromeleonsoftwaremonitorsthelifespanofthelampsandalertsuserswhentochangethem.

Enablesuserstoplanaheadforrequiredmaintenanceincreasingoverallproductivity.

Qualificationperiodissavedinthemodule.Avoidstheuseofunqualifiedequipment,evenwhenthemoduleisswitchedtoanothersystem.

PowerfulChromeleonsoftwaretoolsallowimmediateon-linereviewof3-Ddataandextractionofsinglewavelengthchannelsfromthe3-Dfield.Thesoftware’sadvancedsearchandpeakpurityalgorithmshelpyoutoquicklycreatespectralibrariesandidentifyunknowncompoundsaswellasimpurities.

Makesiteasy,fast,andconvenienttoextracttheinformationyouneed.

PCM-3000upgradeforpHandconductivitymonitoring.ThecombinationofUV-VisdetectorandPCM-3000isidealformonitoringsalt-orpH-gradientseparationsfore.g.monoclonalantibodiesorotherbiomolecules.

3Design OverviewTheDAD-3000opticalbenchdesignincludestwolightsources–deuteriumandtungstenlamp–togetherwith1024diodesandpowerfulelectronics(Figure1).Polychromaticlightpassesthroughtheflowcell.Thedispersedwavelengthsaresimultaneouslydetectedbythediodearray.Thisensuresthesamesensitivityforsingle-andmulti-wavelengthoperation,providingconvenient,optimized,andsensitivequantificationofmaincompoundsandrelatedimpurities.

Reliable QuantificationNoiselevelswhenusingthewideslitare±8 µAUwithatypicalbaselinedriftof<0.5 mAU/hat254nm(flowingmobilephase).Thelownoiseandbaselinedriftallowfordetectionofweaksignals(goodsignal/noiseratio)andconsistent,reproducibleintegration.Atypicaldetectorlinearityupto2.0AUshowscorrelationcoefficientsof>0.999andaresidualstandarddeviationbelow5%withcaffeine.Thewidelinearityrangeallowsreliablequantificationofprimaryandsideproductsinthesamerun.

Sensitivity and Spectral ResolutionWhenacquiringspectraldataforpeakidentificationand/orpeakpurityanalysis,highresolutionspectraareessential.Atthesametime,itisimportanttoobtaingoodsignal-to-noiseratiosfordetectionofweaksignals.TheDAD-3000’sfamilyelectronicsaredesignedtoprovidehighspectralresolutionincombinationwithlownoise.

Figure2showsanexampleofanexplosiveresidueanalysis.Onesamplewasautomaticallyinjectedontwocolumnsofvaryingselectivity.Targetanalyteswereconfirmedbyspectralmatch,evenatverylowconcentrations.Chromeleon’scustomizablereportingfunctionsprovideinstantaneousaccesstoresults,listconfirmedorunconfirmedtargetanalytes,quantifythesubstances,anddefinetheirpeakpurity.

Figure2.DAD-3000examplefollowingEPAmethod8330forexplosivesresidues.Acustomizedreporttakesresultsfrombothseparationsandvisualizesifacompoundisconfirmedandpure.

Figure1.Lightfromthetungstenlampisfocusedthroughanapertureinthedeuterimlamp.Thecombinedlightthenpassesthroughtheflowcellanddiffractiongratingtothediodearray.

Holmium OxideFilter 1024-Element

Diode Array

Flow Cell

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*Only available on DAD-3000RS and MWD-3000RS

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Contaminated soil sampleThermo Scientific™ Acclaim™ Explosives Column E1

Contaminated soil sampleAcclaim Explosives Column E2

4 Rapid Separation LC DetectorsAtypicalpeakrequires30 datapointsforaccurateandpreciseintegration.Up-to-dateUHPLCseparationscanresultinverynarrowanalytepeaks.Thesecanevenreachwidthsofonly0.25s,requiringmorethan100Hzdatacollectionrate.

TheRapidSeparationdiodearraydetectorseasilymeetthisrequirementwithdatacollectionratesofupto200Hz.Thisdetectorperformanceprovideshighlyaccurate,preciseintegrationandretentiontimerecognitionforanyseparationchallenge.

Figure3showshowdetectorswithnon-sufficientdatacollectionratescompromisethepeakintegration.Peakareaprecisionandretentiontimerecognitionareadverselyaffectedatthesametime.

UV Spectra AcquisitionTheDAD-3000RSprovidesuptoeightsingledatachannelsandfullspectralscansat200Hzforeventhemostchallengingapplications.Figure4showstheseparationof10analytes,uracilandninealkylphenones,in10seconds.Thefullspectralscancaneasilykeepupwiththisspeed.PeakidentificationandpeakpurityanalysisareasconvenientandpowerfulasforconventionalLCseparations.Combiningthesefeatureswiththelownoisespecification,theDAD-3000RSisidealforultrafastanalysesandreadytomeetfuturechallenges.

Optimize for Best Sensitivity or Spectral ResolutionBothrapidseparationversions,theDAD-3000RSandtheMWD-3000RS,provideavariableslitattheentranceofthespectrograph.Thisisaneffectivetooltofurtheradaptthedetectortotheapplicationrequirements.Anarrowslitprovidesthehighestspectralresolution,resolvingevenveryfineabsorbancespectra,suchasforpyrene(Figure5).However,UVabsorbancebandsaretypically30nmandwider.Inthesecases,abroadslitresultsinthehighestdetectionsensitivity.Morelightpassesthroughthespectrograph,reducingbothshort-termnoiseandthelimitofdetection.

Figure3.UltrahighdatacollectionratesperfectlysupporttheintegrationofthesharpestUHPLCpeaksforhighestprecisionofquantificationandretentiontime.

Figure4.200HzUVspectraacquisitioneasilysupportstheseparationof10peaksin10seconds.

Figure5.ThevariableslitoftheDAD-3000RSandtheMWD-3000RSisatooltooptimizeforbestspectralresolutionorbestdetectionsensitivity.

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5Rapid Multiple Channel DetectionTheRapidSeparationversionoftheMultipleWavelengthDetectoroffershighspeedandsensitivityforbothsingleandmultiplewavelengthdataacquisition.Itsdiodearraytechnologyachievesmulti-channeldetectionofuptoeightchannelswithoutmovingparts.

Regardlesshowmanywavelengthsarerecorded,thedetectorcanmeasureallwavelengthsatthesamehighsensitivityandatamaximumdatacollectionrateof200Hz.Up-to-datevariablewavelengthdetectorsachievemultiplewavelengthdetectionbyrapidlyswitchingbetweenalternatewavelengths,butthisadverselyaffectsshort-termnoiseandreducesthemaximumdatacollectionratesignificantly.TheMWDdoesnothavethislimitation.

Upgrade MWD to Full DAD FunctionalityMWDdetectorsarebasedonphotodiodearraytechnology.TheonlydifferencefromDADdetectorsisthattheMWDdoesnotsupporttheacquisitionof3-Dspectra.

These3-Dspectracapabilitiesarefield-upgradable,turningyourMWDintoafull-blownDAD.Youmaythereforestaggeryourinvestmentindiodearraytechnology,purchasetheinstrumentyouneedtoday,andadaptittoyourfutureapplicationneedslater.

Ergonomic Design and Advanced System MonitoringAllfluidicpartsoftheMWDandDADdetectorseriescanbeaccesseddirectlyfromthefront.Thedoorfoldsdown,servingasaminiaturelabbenchtoplacetoolsandpartsduringmaintenance(Figure7).

Thedeuteriumandtungstenlampsofthedetectorcanalsobeaccessedfromthefrontbyopeningthefingertightnutsofthelampcover.Changingtheselampsisequallyfastandeasy.ModificationsofflowcellsorlampsareautomaticallytrackedbyintegratedIDchips.AllrelevantinformationisstoredinthelampandmoveswiththedetectorifitistransferredtoanotherLCsystem.Chromeleonconstantlymonitorstheseandvariousothercounterstoprovideyouwithearlywarningswhenapartisdueforreplacement.Inaddition,ChromeleonsoftwaremonitorsandverifiestheinstrumentqualificationstatustomeetyourGLPcompliancegoals(Figure8).

Figure7.Ergonomicinternalfrontdesign(afterremovalofflowcell/lampscovers):directaccessto1)theD2andtungstenlamps,2)theflowcell,and3)theflowcelladapterblockforeasyoperationandmaintenance.

Figure6.Sub-1minsoftdrinkanalysiswithsimultaneousacquisitionofeightdifferentUVchannels,measuredwiththeMWD-3000RS.

Figure8.DAD-3000automatedqualificationmonitoring.

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6 Easy Screening with Chromeleon Spectral LibrariesChromeleonsoftwaresupportseasycreationofspectrallibrariesofknowncompounds(Figure10).

Spectraofunknownpeakscanbecomparedautomaticallyagainstallspectrainalllibraries.Chromeleonsoftwarewillreporttheclosestmatches.Thelibrarycanincludefields,suchassolventcompositionandwavelengthresolution,toprovidemoreadvancedsearchcriteria.

Fast Access to Data and Results via the Peak Purity Analysis ViewViewalldatainasinglesummarywindow,usingadisplaystyleofyourchoice(Figure9).DragtheverticallinetoviewtheassociatedUVspectraatdifferentpointsofthechromatogram.Dragthehorizontallinetoseetheassociatedchromatogramfortheselectedwavelength.Thismakesiteasytofindthebestsignalresponse.Infact,Chromeleonsoftwarecanevenperformthistaskforyouautomatically.Afterthat,itisasimplesteptoextracteitherthechromatogramorthespectrumyouneed.

Getting the Most from Your Spectral Data – with EaseAtypicaldiodearray3-Dfieldcontainsavastamountofinformation.Effectivetoolsareneededtoworkwithsuchvolumesofdata.PowerfulChromeleonsoftwareputsthesetoolsatyourfingertipsandmakesiteasytoextracttheinformationyouneed.WiththeDAD-3000family,youcan:

• Reviewyourresultsinasinglewindow

• Extractsingle-wavelengthchannelsfromyour3-Dfield

• Quicklycreatespectralibrariesforknowncompounds

• Automaticallyidentifyunknownpeaksbasedonasearchofoneormorespectrallibraries

• Easilyidentifypeaksthatcontainunderlyingimpurities,usingadvancedpeakpurityalgorithms

• Effectivelyreporttheresultsinaformatofyourchoice

Figure9.Convenientextractionofasinglewavelength(250nm)from3-DfielddataofaUHPLCseparation.

Figure10.Spectrallibraryscreening.

7Assess Specificity in Seconds with Peak Purity IndexThePeakPurityIndexcanbeoverlaidwithachromatogramforstraightforwardidentificationofpeaksthathaveanunderlyingimpurity(Figure11).

Apurepeakhasaconsistentpeakpurityindex.Anydeviationsindicateanunderlyingimpurity.Thisat-a-glancetechnologycanspeedmethoddevelopmentandhelptoensurespecificity.

Application FlexibilityFourdifferentflowcellsizesareavailablefortheDAD/MWD-3000series:

• AnalyticalflowcellssuitableforHPLCandsomeUHPLCapplications

• Semi-microflowcellsforbestchromatographicresolutionwithsmallIDcolumnsandnarrowanalytepeaks

• Thesemi-analyticalflowcelldesignedfor3mmIDcolumnseparations

• A0.4mmopticalpathlengthsemi-preparativeflowcellforUVdetectionofhigh-concentrationanalytes

Thiswiderangeofflowcellsmeansyoucanadaptyoursystemeasilytoyouranalyticalneeds.

Accurate pH and Conductivity MonitoringThePCM-3000isanupgradeoftheDAD-3000andMWD-3000seriesforaccurateon-linepHandconductivitymonitoring.ThecombinationofUV-ViswiththePCM-3000isidealforpHorsaltgradiention-exchangechromatographyofcompounds,e.g.monoclonalantibodies,conductivitymonitoringingradientseparationsofbiomolecules,andthereforeagreatadditiontotheThermoScientific™Dionex™UltiMate™3000BioRSsystems.

Figure11.Peakpurityexamples.

Figure12.FlowcelldesignofUltiMate3000DiodeArrayDetectors.

Part Number Description Volume (µL)

Optical Path Length (mm)

6082.0100 AnalyticalFlowCell,StainlessSteel 13.0 10.0

6082.0200Semi-AnalyticalFlowCell,StainlessSteel

5.0 7.0

6082.0300 Semi-MicroFlowCell,StainlessSteel 2.5 7.0

6082.0400 AnalyticalFlowCell,PEEK™ 13.0 10.0

6082.0500 Semi-MicroFlowCell,PEEK 2.5 7.0

6082.0600 Semi-PreparativeFlowCell,PEEK 0.7 0.4

Table2.DAD-3000andMWD-3000flowcelloptions

Figure13.ThePCM-3000isahighperformance,easy-to-use,andcosteffectivesolutionforpHandconductivitymonitoring.

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Australia+61397574486Austria+43810282206Belgium+3253734241Brazil+551137315140China+85224283282Denmark+4570236260

Finland+358932910200France+33160924800Germany+4961034081014India+912267429494Italy+3902950591Japan+81668851213

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Taiwan+886287516655UK/Ireland+441442233555USA and Canada+8472957500

Thermo Fisher Scientific, Sunnyvale, CA USA is ISO 9001:2013 Certified.

www.thermoscientific.com/dionex©2013ThermoFisherScientificInc.Allrightsreserved.PEEKisatrademarkofVictrexPLC.ISOisatrademarkoftheInternationalStandardsOrganization.PEEKisatrademarkofVictrexPLC.AllothertrademarksarethepropertyofThermoFisherScientificInc.anditssubsidiaries.Specifications,termsandpricingaresubjecttochange.Notallproductsareavailableinallcountries.Pleaseconsultyourlocalsalesrepresentativefordetails.

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PS70286_E07/13S

Specifications

Dimensions(h×w×d) 16×42×51cm(6.3×16.5×20in.)

DetectionType Single-beam,reverse-opticsdesignwithconcaveholographicgrating

MaximumData 200Hz:DAD-3000RS(including3-Dacquisition)CollectionRate) andMWD-3000RS(underChromeleon7.1software

control)100Hz:DAD-3000(including3-Dacquisition)andMWD3000

WavelengthRange 190to800nm

Noise Wideslit:<±8μAUat254nm.Narrowslit:<±10μAUat254nmresponsetime2s(accordingtoASTMtimeconstant~1s),4nmbandwidth,waterat1.0mL/min

Drift <1mAU/h(typically<0.5mAU/h)at254and520nmwithwaterat1.0mL/min

Linearity <3%RSDandcorr.coeff.>0.9995upto1.5AU,typically<5%RSDandcorr.coeff.>0.999upto2.0AU

LightSource Deuteriumlamp,tungstenlamp,temperaturecontrolforbothlamps

WavelengthAccuracy ±1.0nm,self-calibrationwithD-alphaline,verificationwithholmiumoxidefilter

WavelengthPrecision ±0.1nm

PixelResolution <1nm

SlitWidth Narroworwideslit,settablefortheDAD-3000RSandMWD-3000RS

PCConnection AllfunctionscontrollableviaUSB2.0;integratedUSBhubwiththreeUSB2.0ports

GLPFeatures AutomaticInstrumentQualification(AutoQ),SystemWellnessandQualificationMonitoringwithChromeleonsoftware,lampandcellIDchips

UserInput/Display LCDindicatingsystemparameters,standbybutton,threeLEDsforstatusmonitoring,fourfunctionkeysforinitialoperationandmaintenance

PowerRequirements 85-260V,50/60Hz,max.150W

Ordering Information

ToorderintheU.S.,call1-800-346-6390,orcontacttheThermoFisherScientificofficenearestyou.OutsidetheU.S.,orderthroughyourlocalThermoFisherScientificofficeordistributor.Refertothefollowingpartnumbers.

Detectors Part Number

DAD-3000RSRapidSeparationDiodeArrayDetector 5082.0020(withoutflowcell)

DAD-3000DiodeArrayDetector(withoutflowcell) 5082.0010

MWD-3000RSRapidSeparationMultipleWavelengthDetector 5082.0040(withoutflowcell)

MWD-3000MultipleWavelengthDetector(withoutflowcell) 5082.0030

Accessories Part Number

Analyticalflowcell,stainlesssteel,13μL 6082.0100

Semi-analyticalflowcell,stainlesssteel,5μL 6082.0200

Semi-microflowcell,stainlesssteel,2.5μL 6082.0300

Analyticalflowcell,PEEK,13μL 6082.0400

Semi-microflowcell,PEEK,2.5μL 6082.0500

Semi-preparativeflowcell,PEEK,0.7μL 6082.0600

UpgradeMWD-3000RStoDAD-3000RS 6082.3045

UpgradeMWD-3000toDAD-3000 6082.3035

PCM-3000forDAD-3000orMWD-3000Series 6082.2000(ShippedwithapHandconductivityflowcellandapHelectrode)

Deuteriumlamp 6074.1110

Tungstenlamp 6074.2000