Bioanalytical Solutions

Bioanalytical Systems

UltiMate™ 3000Inert BioLC—Nano to MicroAnalytical to Semiprep HPLC ICS-3000™

Metal-Free BioIC/BioLCAnalyticalAPS-2000 and APS-3000

Maximized AutopurificationSemiprep to Preparative DX-800™ PAT

Process IC and LCAnalytical

Bioanalytical SystemsPerformance, Reliability, and Ease-of-Use





Nano/Cap/Micro, inert Binary Micro, inert Quaternary Micro, inert Binary Analytical Quaternary Analytical Binary Semi-prep

UltiMate™ 3000 LC

UltiMate™ 3000 LC

New for 2007!

Well Plate Sampler with Fraction Collection

Pharma/Biotech Columns

ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®

High Efficiency for Oligos

Acclaim®

Tailored Selectivity (Si)

ProPac®

Protein Separations

CarboPac®

Mono to Oligosaccharides


ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®


Acclaim®


ProPac®

Protein Separations

CarboPac®


Acclaim®

Fast LC

New for 2007!

New Acclaim® Fast LC Columns

4.6 x 150 mm

3 x 75 mm

3 x 33 mm

15x Speed with Equivalent Resolution

12 minutes

2.7 minutes

0.8 minutes

UltiMate™ 3000 LC

Nano/Capillary/Micro Binary MicroQuaternary Micro

All with x2 Dual Technology

Designed for Better Mass Spectrometry


ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®


Acclaim®


ProPac®

Protein Separations

CarboPac®



ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®


Acclaim®


ProPac®

Protein Separations

CarboPac®


Acclaim®

Mixed ModeWAX-1

New for 2007!

Acclaim® Mixed-Mode WAX-1

Simultaneous Separation of Acidic, Basic, and Neutral Molecules


Move Only the Anion When Adjusting the Ionic Strength

Adjustable Selectivity: Ionic StrengthpHOrganic Modifier


Move Only the Anion When Adjusting the pH



Total Control of Selectivity



Selectivity Orthogonal to Reversed-Phase Column

Bioanalytical Solutions

UltiMate™ 3000Inert LC—Nano to MicroAnalytical to Semiprep HPLC ICS-3000™

Metal-Free BioIC/BioLCMicrobore to AnalyticalAPS-2000 and APS-3000




Bioanalytical SystemsPEEK inert IC and LC

UltiMate™ 3000Inert LC—Nano to MicroAnalytical to Semiprep HPLC

APS-2000 and APS-3000Maximized AutopurificationSemiprep to Preparative DX-800™ PAT


ICS-3000™

Metal-Free BioIC/BioLCMicrobore to Analytical

ICS-3000 IC and LC System

Microbore to analytical flow

Metal-free PEEK flow pathSingle or dual pumpMultiple detector options

ICS-3000 3D Amperometry

PDA-type information

with the unique selectivity of electro-chemical detection


The derivatization-free solutions for – Amino acids– Carbohydrates– Sugar alcohols– Amino sugars

Perfectly inert– To protect

bioactivity


Perfectly inert– To handle corrosive

mobile phases


ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®


Acclaim®


ProPac®

Protein Separations

CarboPac®



ProSwift™

Monolith Technology

AminoPac®

AAA-Direct™

DNAPac®


Acclaim®


ProPac®

Protein Separations

CarboPac®


ProSwift™

SAX and WCX

New for 2007!

ProSwift™ Monolith Morphology

High Permeability Low Backpressure High Flow RateFlow-Through Pores Fast Mass Transfer High Resolution

Non-porous GlobulesThrough Pores

ProSwift™ SAX Column: Protein Mix

Column: SAX (4.6 x 50 mm)Flow Rate: 1.5 mL/minEluents: A. 10 mM Tris (pH 7.6)

B. 10 mM Tris + 1M NaCl (pH 7.6)

Monolith Technology for Speed and Efficiency

–1000 9

900

50.0

100.0

%B

1

2

3

4

1. Myoglobin 2. Conalbumin 3. Ovalbumin4. Trypsin inhibitor

5 minutes

Minutes

ProSwift™ WCX Column: MAb Variants

Monolith Technology for High Capacity Separations

MAb 5 mg/mL

Column: WCX (4.6 x 50 mm)Flow Rate: 1 mL/minEluents: A. 10 mM Na2HPO4

(pH 7.6)B. 1M NaCI in eluent AmAU

Minutes0 14.1–100

600

10.0

100.0

5.2%B

1

2

3

Basic VariantsAcidic Variants

Lys Variants

Bioanalytical SystemsPerformance, Reliability, and Ease-of-Use

ICS-3000™




UltiMate™ 3000Inert BioLC—Nano to MicroAnalytical to Semiprep HPLC

x2 Dual-Nano/Cap/Micro

x2 Dual-Binary Micro

x2 Dual-Ternary Micro

x2 Dual-Binary Analytical

x2 Dual-Ternary Analytical

x2 Dual-Binary Semiprep

x2 Dual LC Systems

UltiMate™ 3000 x2 Dual HPLC

More productivity– Tandem column mode– Parallel column mode

Increased use-time– Application switching

On-line sample prep– Heartcut mode – Backflush mode

Higher selectivity– Multidimensional LC

x2 Dual-Channel Parallel Mode

0 20Minutes

Analysis Channel 1

Analysis Channel 2

Without Changing Validated Methods

Increase Throughput by 100%

Use the Full Power of Reliable 2-D LCTCC 100 MSV 2P 10P

Pump #1

Pump #2

IV

DetectorUV

214 nm

Fe3+ IMAC

Acclaim C18

w

w

Collection Loop 400 µL

10-Port Valve

UltiMate 3000

x2 Dual-Ternary

HCO2H, pH 3

NH4HCO2H, pH 9

5% MeCN, 0.1% TFA

70% MeCN, 0.1% TFA

0 1 2 0 1 2Minutes Minutes

Step 1IMAC FractionNon-phospho-

peptides

Step 3IMAC Fraction

Phosphopeptides

5 10 15Minutes

Step 4

RPC of Phosphopeptide Fraction

5 10 15Minutes

Step 2RPC of Non-phosphopeptide Fraction

Simplify and Improve Sample PreparationDirect Injection of Human Plasma

0 2 4 6 8 10 13–10

0

10

20

30

40

50

mAU

min

Separation on Analytical Column

Minutes

RAM (RP/SEC)Fractionation

Transfer from SPE Column

Pump BPump A

Dual GradientPump

Autosampler

RAMColumn

AnalyticalColumn

RAM: Restricted Access Material

UV-Vis Detector

VoriconazoleKetoconazole

1-OH-ItraconazoleItraconazole

Wash SPE Column

ReconditionSPE Column

Chromeleon® Control of Every Component

Bioanalytical Solutions - Dionex

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