Microwave Acid Digestion Method Note...

MARS 6Microwave Acid Digestion

Method Note Compendium

Updated January 31, 2018

Contents

Agriculture

Boric Acid HF Neutralization 2

Coffee Beans 3

Cotton 4

Feed Grain 5

Fertilizer AOAC Method 2006.03 6

Plant Tissue 7

Soybean 8

Spinach Leaves 9

Straw 10

Tobacco 11

Wheat 12

Yeast 13

Clinical and Biological

Animal Tissue (Dry) 15

Animal Tissue (Wet) 16

Blood (Human) 17

Bovine Liver (Wet) 18

Hair 19

Lobster Hepatopancreas (Tort2-CRM) 20

Urine (Human) 21

Consumer Products

Cosmetics (Liquid Foundation) 24

Cotton 25

Eye Shadow 26

Shampoo (Dandruff) 27

Suppository (Capsule) 28

Vaseline 29

Wax (Candle) 30

Environmental and Regulatory

Fertilizer - AOAC Method 2006.03 33

Limestone 34

NPDES (Wastewater) 35

Pine Needles 36

RoHS (For Pb, Hg, and Cd Analysis) 37

Sediment (BCCS-1 CRM) (Leach) 38

Sediment (Buffalo River) (Leach) 39

Sludge (Industrial) 40

Sludge (Waste Activated) 41

Soil (Montana-CRM) (Leach) 42

TCLP Extract 43

US EPA 3015 (Aqueous Samples) 44

US EPA 3015a (Aqueous Samples) 45

US EPA 3051 (Solid Samples) 46

US EPA 3051a (Solid Samples) 47

US EPA 3052 48

USP 232/233 (Pharmaceuticals) 49

Water (for analysis of Phosphorus) 50

Wood 51

Food Testing

Apple 53

Asparagus 54

Bacon (Cooked Pieces) 55

Beef (Ground) 56

Beer (Light) 57

Blueberry 58

Broccoli 59

Caramel Color 60

Carrot 61

Celery 62

Cheese Crackers 63

Cheese (Processed) 64

Cherry 65

Chewing Gum 66

Chicken (Boneless) 67

Chili Candy (Mexican) 68

Chips (Potato) 69

Coffee Beans 70

Cucumber 71

Dog Food (Dry) 72

Dog Food (Wet) 73

Flour 74

Food Coloring (Liquid) 75

Food 76

Fruit Juice 77

Granola Bar 78

Grape 79

Grapefruit 80

Ham 81

Infant Cereal (Rice Grain) 82

Infant Formula (Liquid) 83

Infant Formula (Liquid) in iPrep Vessels 84

Infant Formula (Powder) 85

Infant Formula (Powder) in iPrep Vessels 86

Kidney Bean 87

Maple Syrup 88

Mayonnaise 89

Milk (Liquid, Whole) 90

Milk (Powder) 91

Nutritional Drink (Adult) 92

Olive Oil 93

Orange 94

Orange Juice 95

Peanut Butter 96

Pear 97

Pepper (Bell, Chili, etc.) 98

Pizza (Frozen, Pepperoni) 99

Plum 100

Pork Gelatin 101

Pork (Ground) 102

Potato 103

Pretzel (Salted) 104

Protein Bar 105

Raspberry 106

Rice 107

Safflower Oil 108

Salami 109

Sausage 110

Soda (Diet) 111

Soybean 112

Spinach 113

Spinach Leaves 114

Strawberry 115

Sugar (Granulated) 116

Tea Leaves 117

Tomato Leaves 118

Tomato Soup 119

Walnut (Ground) 120

Watermelon 121

Whey Powder 122

Wine 123

Yogurt (Plain) 124

Geoscience and Mining

Alpha - Alumina in iPrep Vessels 126

Aluminum Oxide 127

Automotive Catalyst 128

Bauxite (Step 1 of 2) 129

Bauxite (Step 2 of 2) 130

Carbon 132

Carbon Nanotubes 133

Cement 134

Ceramic (Fused Silica) 135

Ceramics 136

Char (Sulfuric Acid) 137

Clay 138

Coal 139

Coal in iPrep Vessels 140

Coal Ash 141

Diesel Fuel 142

Iron Ore 143

Kerosene 144

Limestone 145

Mill Tailings (Step 1 of 2) 146

Mill Tailings (Step 2 of 2) 147

Motor Oil (Waste) 148

Petroleum Coke (Step 1 of 2) 149

Petroleum Coke (Step 2 of 2) 150

Rock (Pulverized) 151

Rutile Ore 152

Sand 153

Silica Sand 154

Silicon Dioxide 155

Silicon Wafer 156

Slag (Furnace) 157

Titanium Dioxide 158

Tungsten Carbide 159

Tungsten Oxide 160

Zeolite 161

Industrial Hygiene

Filter Paper (Cellulose) 163

Filter (37 mm Mixed Cellulose Ester) 164

Filter (47 mm Polycarbonate) 165

Ghost Wipe 166

Toray Filter in iPrep Vessels 167

Materials Science

Circuit Board (Cryo-ground) 169

Glass (Ground) 170

Graphite Fiber (Epoxy Resin, Fiber Content) 171

Metals and Alloys

Alluminum Alloy 173

Alpha - Alumina in iPrep Vessels 174

Aluminum Oxide 175

Aluminum (Metal) 176

Copper (Metal) 177

Copper (Ore) 178

Metal Alloy (CoCr) (Step 1 of 2) 179

Metal Alloy (CoCr) (Step 2 of 2) 180

Metal Alloy (FeCr) 181

Metal Alloy (NiCr) 182

Nickel (Metal) 183

Platinum (Metal) 184

Precious Metal (Ore) (Step 1 of 2) 185

Precious Metal (Ore) (Step 2 of 2) 186

Selenium Alloy 187

SnAg Solder 188

Stainless Steel 189

Steel (Stainless) 190

Titanium Alloy 191

Titanium Dioxide 192

Zinc Oxide 193

Nutraceuticals

Biotin 195

Ginko (Ground) 196

Ginko (Whole Pill) 197

Infant Formula (Liquid) 198

Infant Formula (Liquid) in iPrep Vessels 199

Infant Formula (Powder) 200

Infant Formula (Powder) in iPrep Vessels 201

Kelp 202

Milk (Liquid, Whole) 203

Milk (Powder) 204

Plant Tissue 205

Wheat 207

Whey Powder 208

Organic Chemicals

Photoresist 210

Paints and Coatings

Paint (Latex Based Liquid) 212

Paint Chips (For Pb Analysis) 213

Photoresist 214

Toner (Ink) in iPrep Vessels 215

Personal Care (Health and Beauty)

Cosmetics (Liquid, Foundation) 218

Eye Shadow 219

Shampoo (Dandruff) 220

Pharmaceutical and Biotech

Allergy Pill (Ground) 222

Allergy Pill (Whole Pill) 223

Antioxidant (Ground) 224

Antioxidant (Whole Pill) 225

APIs with Aromatic Ring Structures in iPrep Vessels 226

Ascorbic Acid 227

Aspirin (Ground) 228

Aspirin (Whole) 229

Beta Carotene 230

Biotin 231

Calcium Carbonate 232

Calpan Pantothenic Acid 233

Cephalexin 234

Chromium Chelate 235

Cold and Flu Medicine (Liquid) 236

Cupric Sulfate 237

Diclofenac K 238

Empty Capsule (Gel) 239

Fish Oil (No Capsule) 240

Folic Acid 241

Ginko (Ground) 242

Ginko (Whole Pill) 243

Kelp 244

Losataran K 245

Magnesium Carbonate 246

Magnesium Oxide 247

Manganese Carbonate 248

Metaformin (Ground) 249

Multivitamin (Ground) 250

Multivitamin (Whole) 251

Niacinamide 252

Pantothenic Acid 253

Potassium Chelate 254

Pyridoxine 255

Riboflavin 256

Selenium Chelate 257

Stearic Acid 258

Thiamine 259

Vitamin B-12 261

Vitamin C (Ground) 262

Vitamin C (Whole) 263

Vitamin D 264

Vitamin D3 265

Vitamin E 266

Vitamins 267

Zinc Sulfate 268

Plastics, Polymers, and Oils

Acrylamide 270

Bunker Oil 271

Bunker Oil in iPrep Vessels 272

Char (Sulfuric Acid) 273

Crude Oil 274

Diesel Fuel 275

Epoxy Hardener 276

Fatty Alcohol 277

Gasoline 278

HDPE (High Density Polyethylene) 279

Kerosene 280

Kevlar in iPrep Vessels 281

Lube Oil 282

Mineral Oil 283

Motor Oil (Waste) 284

Motor Oil (Waste) in iPrep Vessels 285

Nylon 286

PET (Polyethylene Terephthalate) 287

PET (Polyethylene Terephthalate) in iPrep Vessels 288

Polycarbonate Resin (Step 1 of 2) 289

Polycarbonate Resin (Step 2 of 2) 290

Polyethersulfone (Filter Paper) 291

Polypropylene 292

Polyurethane 293

PVC (Polyvinyl Chloride, Resin) 294

Rubber 295

Terephthalic Acid 296

Pulp and Paper

Filter Paper (Cellulose) 298

Filter (37 mm Mixed Cellulose Ester) 299

Filter (47 mm Polycarbonate) 300

Paper Pulp 301

Water and Wastewater Treatment

NPDES (Wastewater) 303

Sludge (Industrial) 304

Sludge (Waste Activated) 305

TCLP Extract 306

US EPA 3015 (Aqueous Samples) 307

US EPA 3015a (Aqueous Samples) 308

Water (for Analysis of Phosphorus) 309

Agriculture

Procedure

Recommended Equipment Recommended Vessels

Microwave Digestion of

Heating Program

Ramp (mm:ss) Hold (mm:ss) Pressure (psi) Power (W) StirringStage Temp (°C)

Control Type Method Type

Reagents

Max Sample Weight Sample Type

www.cem.com Form prepared by:

MARS 6TM

Method Note

Results

General Precautions

* Ramp times and power may vary depending on type and number of vessels.

Boric Acid HF Neutralization

Allow vessel to cool. Add 30 ml H3BO3 (4%) into the vessel that contains the sample and acid.

This procedure can be used if it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed by reaction ofcertain analytes with hydrofluoric acid.

Alternatively, 1-4g of solid H3BO3 + 25mL deionized H2O can be used in place of the 4% w/v solution.

MARS 6MARS 6 iWave

EasyPrepEasyPrep Plus

H3BO3 (4%)

Varies by Sample Organic Ramp to Temperature One Touch

170 15:00 15:00 800 900-1050 Off

See sample specific method notes.

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBoric Acid HF Neutralization

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Coffee Beans

Weigh 0.5 g of ground sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpressXpress Plus

0.5 g Organic Ramp to Temperature One Touch

200 20:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 ml.

Microwave Digestion ofCoffee Beans

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cotton

Weigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep Plus

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL.

Microwave Digestion ofCotton

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Feed Grain

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3, and 2 ml of HCl. Gently swirl the mixtureand wait approximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

XpressXpress PlusEasyPrepEasyPrep Plus

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFeed Grain

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Fertilizer-AOAC Method 2006.03

Weigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

This method may not provide a total digest of all fertilizer samples. Hydrofluoric acid will be required to provide complete digestion of somesample matrixes.

MARS 6MARS 6 iWave

HNO3HCl

1 g Organic Ramp to Temperature One Touch

200 15:00 20:00 800 900-1050 Off

Microwave Digestion ofFertilizer AOAC Method 2006.03

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Plant Tissue

Weigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

Some plant tissues contain silicates which would require HF for total dissolution.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPlant Tissue

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Soybean

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSoybean

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Spinach Leaves

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSpinach Leaves

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO3, and 1 ml of HF. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluoridesformed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled“Boric HF Neutralization”

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofStraw

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tobacco

The use of HF may be required to digest any silicates found in the sample.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTobacco

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWheat

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 1.0 g of dry weight (0.5 g w/Xpress vessels) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofYeast

Clinical and Biological

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Animal Tissue (Dry)

Weigh 0.5 g (0.25 g w/Xpress Vessels) into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAnimal Tissue (Dry)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Animal Tissue (Wet)

Weigh 1 g wet weight (0.5 g w Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAnimal Tissue (Wet)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Blood (Human)

Weigh 2 ml of the sample into the digestion vessel. Add 5 ml of HNO3 and 2 ml of H2O2. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

Take caution when mixing the acids and the sample. The sample may foam slightly.

MARS 6MARS 6 iWave

HNO3H2O2

2 ml Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBlood (Human)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Bovine Liver (Wet)

Weigh 1.0 g wet weight (0.5 g w/Xpress Vessels) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBovine Liver (Wet)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.25 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

180 15:00 15:00 800 900-1050 Off

Sample was clear, colorless and particle free upon dilution to 50 ml.

Microwave Digestion ofHair

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Lobster Hepatopancreas (Tort-2 CRM)

Weigh 1.0 g dry weight (0.5 g w/Xpress Vessels) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofLobster Hepatopancreas (Tort2-CRM)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Urine (Human)

Transfer 4 ml of the sample into the digestion vessel. Add 4 ml of HNO3, and 2 ml of H2O2. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

Take caution when mixing the acids and the sample. The sample may foam slightly.

MARS 6MARS 6 iWave

HNO3H2O2

4 mL Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofUrine (Human)

Consumer Products

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

H3BO3 (4%)

170 15:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cosmetics (Liquid Foundation)

Weigh 1 g of the sample into the digestion vessel. Add 8 ml of HNO3 and 2 ml of HF. Gently swirl the mixture before closing the vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, anadditional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric AcidHF Neutralization”.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCosmetics (Liquid Foundation)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cotton

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCotton

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Eye Shadow

Weigh 0.25 g of the sample into the digestion vessel. Add 7 mL of HNO3 and 3 mL of HF. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HFHNO3

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofEye Shadow

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Shampoo (Dandruff)

Add 1 mL of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofShampoo (Dandruff)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Suppository (Capsule)

Weigh 1 Capsule (approx. 1.0 g) into the digestion vessel. Add 12 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

Ensure that the capsule is completely covered with reagent before sealing the vessel.

MARS 6MARS 6 iWave

1 Capsule (approx 1 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSuppository (Capsule)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vaseline

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofVaseline

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Wax (Candle)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWax (Candle)

Environmental and Regulatory

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

H3BO3 (4%)

170 15:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Fertilizer - AOAC Method 2006.03

Weigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

This method may not provide a total digest of all fertilizer samples. Hydrofluoric acid will be required to provide complete digestion of somesample matrixes.

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 20:00 800 Off

Microwave Digestion ofFertilizer - AOAC Method 2006.03

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Limestone

Weigh 0.5 g of the sample into the digestion vessel. Add 7.5 ml of HNO3 and 2.5 ml of HF. Gently swirl the mixture before closing the vessel.

Allow any initial reaction to subside before sealing the vessel.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofLimestone

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

NPDES (Waste Water)

Transfer 50 ml of the sample into the digestion vessel. Add 3 ml of HNO3, and 2 ml HCl. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

Xpress (75 ml)Xpress PlusEasyPrepEasyPrep Plus

HNO3HCl

50 ml Water Ramp to Temperature One Touch

165 30:00 0:00 800 900-1050 Off

Microwave Digestion ofNPDES (Wastewater)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pine Needles

Weigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2O2 (30%)

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPine Needles

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

RoHS (For Pb, Hg, and Cd Analysis)

Weigh 0.2 g of the sample into the digestion vessel. Add 10 ml of HNO3, and 0.02 ml of H2SO4. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2SO4.

200 10:00 15:00 800 900-1050 Off

Microwave Digestion ofRoHS (For Pb, Hg, and Cd Analysis)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sediment (BCCS-1 CRM) (Leach)

This method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some samplematrixes.

MARS 6MARS 6 iWave

180 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSediment (BCCS-1 CRM) (Leach)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sediment (Buffalo River) (Leach)

Weigh 0.5 g into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing thevessel.

This method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some samplematrixes.

MARS 6MARS 6 iWave

175 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSediment (Buffalo River) (Leach)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sludge (Industrial)

Weigh 0.5 g (dry weight) of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

0.5 g (dry weight) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSludge (Industrial)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sludge (Waste Activated)

Weigh 0.5 g dry weight (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 5 ml of HNO3, and 5 ml of H2O2. Gently swirlthe mixture and wait approximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

MARSXpressMARSXpress PlusEasyPrepEasyPrep Plus

HNO3H2O2

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSludge (Waste Activated)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Soil (Montana-CRM) (Leach)

This method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide completedigestion of some sample matrixes.

MARS 6MARS 6 iWave

175 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSoil (Montana-CRM) (Leach)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

TCLP Extract

Transfer 25 ml of the sample into the digestion vessel. Add 5 ml of HNO3. Gently swirl the mixture before closing the vessel.

If a high organic content is suspected, a 10 ml sample volume is recommended.

MARS 6MARS 6 iWave

EasyPrep PlusXpress (75 ml)Xpress Plus

25 mL EPA Digestion (Water) Standard Control One Touch

170 10:00 10:00 800 900-1050 Off

Microwave Digestion ofTCLP Extract

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3015 (Aqueous Samples)

MARS 6MARS 6 iWave

EasyPrep PlusXpress (75 ml)XpressPlus

45 ml Environmental Digestion(Water)

Standard Control One Touch

170 10:00 10:00 800 900-1050 Off

This method is intended to be an acid leach, not a total digest. Hydrofluoric acid will be required to provide complete digestion of the samplematrix.

Microwave Digestion ofUS EPA 3015 (Aqueous Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3015a (Aqueous Samples)

Transfer 45 ml of the sample into the digestion vessel. Add 5 ml of HNO3, or alternatively 4 ml of HNO3 and 1 ml HCl. Gently swirl themixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl (Optional)

170 10:00 10:00 800 900-1050 Off

Microwave Digestion ofUS EPA 3015a (Aqueous Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3051 (Solid Samples)

MARS 6MARS 6 iWave

EasyPrep PlusXpressXpressPlus

HNO3HCl (Optional)

0.5 g Environmental Digestion(Solids)

175 5:30 4:30 800 900-1050 Off

This method is intended to be an acid leach, not a total digest. Hydrofluoric acid will be required to provide complete digestion of the samplematrix. (See method 3052 for total digestion).

Microwave Digestion ofUS EPA 3051 (Solid Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3051a (Solid Samples)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO3, or alternatively 9 ml HNO3, and 1 ml HCl. Gently swirl the mixturebefore closing the vessel.

MARS 6MARS 6 iWave

EasyPrep PlusXpressXpressPlus

HNO3HCl (Optional)

175 5:30 4:30 800 900-1050 Off

This method is intended to be an acid leach, not a total digest. Hydrofluoric acid will be required to provide complete digestion of the samplematrix. (See method 3052 for total digestion)

Microwave Digestion ofUS EPA 3051a (Solid Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3052

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 3 ml HF. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

EasyPrep PlusXpressXpress Plus

HNO3HF

180 5:30 9:30 800 900-1050 Off

Microwave Digestion ofUS EPA 3052

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

USP 232/233 (Pharmaceuticals)

Weigh 0.2 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml HCl. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofUSP 232/233 (Pharmaceuticals)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Water (for analysis of Phosphorus)

Weigh 50 mL of H2O into the digestion vessel. Add 0.5 g of K2S2O8 and 1 mL of H2SO4. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6 XpressXpressPlusEasyPrepEasyPrep Plus

K2S2O8H2SO4

170 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWater (for analysis of Phosphorus)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWood

Food Testing

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofApple

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Asparagus

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAsparagus

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Bacon (Cooked Pieces)

Weigh 1.0 g of the sample into the digestion vessel. Add 8 mL of HNO3 and 2 mL of DI H2O. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3DI H2O

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBacon (Cooked Pieces)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Beef (ground)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBeef (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Beer (Light)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBeer (Light)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Blueberry

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBlueberry

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Broccoli

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBroccoli

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Caramel Color

Weigh 2.0 mL of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

2.0 mL Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCaramel Color

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Carrot

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCarrot

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Celery

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCelery

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cheese Crackers

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofCheese Crackers

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cheese (Processed)

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofCheese (Processed)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cherry

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCherry

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Chewing Gum

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofChewing Gum

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Chicken (Boneless)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChicken (Boneless)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Chili Candy (Mexican)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChili Candy (Mexican)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Chips (potato)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChips (Potato)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Coffee Beans

MARS 6MARS 6 iWave

200 20:00 15:00 800 900-1050 Off

Microwave Digestion ofCoffee Beans

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cucumber

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCucumber

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Dog Food (Dry)

Weigh 0.5 g of sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofDog Food (Dry)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Dog Food (Wet)

Weigh 1 g of wet sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofDog Food (Wet)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFlour

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Food Coloring (liquid)

Add 0.5 mL of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFood Coloring (Liquid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.5 g dry weight (0.25 g w/Xpress vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture andwait approximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFood

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Fruit Juice

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFruit Juice

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Granola Bar

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofGranola Bar

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGrape

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Grapefruit

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGrapefruit

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofHam

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Cereal (Rice Grain)

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofInfant Cereal (Rice Grain)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Liquid)

Transfer 1 ml (0.5 ml w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the requiredresults on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofInfant Formula (Liquid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Liquid) in iPrep Vessels

Transfer 2 ml of of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

This application can only be run in the iPrep vessel.

MARS 6 iWave iPrep HNO3

210 20:00 20:00 N/A 500-1800 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofInfant Formula (Liquid) in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Powder)

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofInfant Formula (Powder)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Powder) in iPrep Vessels

Weigh 1 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

210 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofInfant Formula (Powder) in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Kidney Bean

Weigh 0.5 g (dry weight) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofKidney Bean

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Maple Syrup

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofMaple Syrup

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Mayonnaise

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofMayonnaise

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Milk (Liquid, Whole)

Transfer 4 ml of the liquid sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMilk (Liquid, Whole)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Milk (Powder)

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofMilk (Powder)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Nutritional Drink (adult)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofNutritional Drink (Adult)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Olive Oil

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofOlive Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Orange

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofOrange

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Orange Juice

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofOrange Juice

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Peanut Butter

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofPeanut Butter

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPear

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pepper (Bell, Chili, etc.)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPepper (Bell, Chili, etc.)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pizza (Frozen, Pepperoni)

Weigh 1.0 g (homogenized) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPizza (Frozen, Pepperoni)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPlum

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pork Gelatin

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPork Gelatin

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pork (ground)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPork (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Potato

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPotato

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pretzel (salted)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPretzel (Salted)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Protein Bar

Weigh 0.5 g ground sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofProtein Bar

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Raspberry

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofRaspberry

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofRice

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Safflower Oil

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSafflower Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Salami

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpress (75 ml)Xpress Plus

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSalami

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sausage

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpress (75 ml)Xpress Plus

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSausage

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Soda (Diet)

Add1 mL of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSoda (Diet)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Soybean

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSoybean

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Spinach

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSpinach

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Spinach Leaves

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSpinach Leaves

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Strawberry

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofStrawberry

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sugar (granulated)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSugar (Granulated)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tea Leaves

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTea Leaves

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tomato Leaves

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, with white silica particles upon dilution to 50 mL.

Microwave Digestion ofTomato Leaves

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tomato Soup

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTomato Soup

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Walnut (ground)

Weigh 0.5 g (dry weight) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWalnut (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Watermelon

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWatermelon

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Whey Powder

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofWhey Powder

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Transfer 1 ml of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofWine

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Yogurt (Plain)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofYogurt (Plain)

Geoscience and Mining

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Alpha - Alumina in iPrep Vessels

Weigh 0.5 g of sample into the digestion vessel. Add 10 ml HCl. Gently swirl the vessel to thoroughly mix the sample and acid.

Ensure that particles are well suspended in acid mixture prior to sealing vessel.

Stirring may be helpful to keep particles suspended in solution during digestion.

MARS 6 iWave iPrep HCl

240 30:00 60:00 N/A 1800 N/A

Microwave Digestion ofAlpha - Alumina in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aluminum Oxide

Weigh 0.25 g of sample into the digestion vessel. Add 6.5 ml H3PO4 and 3.5 ml H2SO4. Gently swirl the vessel to thoroughly mix thesample and acid.

EasyPrep Plus requires a high temperature probe for this method.

MARS 6MARS 6 iWave

Easy PrepEasy Prep Plus

H3PO4H2SO4

270 30:00 20:00 N/A 1800 N/A

Microwave Digestion ofAluminum Oxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Automotive Catalyst

Weigh 0.4 g of the sample into the digestion vessel. Add 10 ml of HCl and 0.5 ml of HF. Gently swirl the mixture before closing the vessel.

Reducing the particle size increases the efficiency of digestion and may decrease the digestion time.

MARS 6MARS 6 iWave

210 15:00 30:00 800 900-1050 Off

Microwave Digestion ofAutomotive Catalyst

Step 1 of 2

www.cem.com

Procedure

Heating Program

Reagents

Microwave Digestion ofMARS 6TM

Method Note

Control Type Method TypeMax Sample Weight Sample Type

Bauxite

Weigh 0.25 g of the sample into the digestion vessel. Add 6.5 mL of H3PO4 and 3.5 mL of H2SO4. Gently swirl the mixture before closingthe vessel.

MARS 6MARS iWave

H3PO4H2SO4

240 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBauxite (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Method NoteMicrowave Digestion of

Results

General Precautions

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on yoursample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Bauxite

Proceeding step 1 add 1 mL of HNO3, 1 mL of HCl, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

HNO3HClHF

200 15:00 15:00 800 900-1050 Off

Sample was clear, colorless, and particle free upon dilution to 50 mL

Microwave Digestion ofBauxite (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

H3BO3 (4%)

170 15:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Carbon

Weigh 0.1 g of the sample into the digestion vessel. Add 10 ml of HNO3 . Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

The structure, form and surface area of carbon samples varies widely. Higher temperatures and use of other reagents may be necessary inorder to digest certain samples.

MARS 6MARS 6 iWave

215 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCarbon

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Carbon Nanotubes

MARS 6MARS 6 iWave

220 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCarbon Nanotubes

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cement

Weigh 0.3 g of the sample into the digestion vessel. Add 3 ml of HNO3 , 6 ml of HCL, and 3 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

HNO3HClHF

175 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCement

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ceramic (Fused Silica)

Weigh 0.5 g of the sample into the digestion vessel. Slowly add 2 ml of HNO3 , and 8 ml of HF. Gently swirl the mixture before closing thevessel.

HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel.

MARS 6MARS 6 iWave

HNO3HF

190 10:00 20:00 800 900-1050 Off

Microwave Digestion ofCeramic (Fused Silica)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ceramics

Weigh 0.2 g of the sample into the digestion vessel. Add 5 ml of HNO3, and 5 ml of HF. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCeramics

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Char (Sulfuric Acid)

Add 5 ml H2SO4 into the vessel that contains the sample and acid.

This method is for the pretreatment of large sample sizes or difficult organic samples that are resistant to oxidation. After the char iscomplete, the vessel is opened and a normal oxidation with HNO3 can be run, usually at around 200C.

MARS 6MARS 6 iWave

260 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChar (Sulfuric Acid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.2 g of the sample into the digestion vessel. Add 3 ml of HNO3, 1 ml of HCl, and 5 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

HFHNO3HCl

200 15:00 10:00 800 900-1050 Off

Microwave Digestion ofClay

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Coal (Anthracite)

After heating program in step1, allow vessel to cool before proceeding with step 2.

MARS 6MARS 6 iWave

EasyPrepEasy Prep Plus

200 15:00 20:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the the number and type of vessels detected.

Microwave Digestion ofCoal

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Coal in iPrep Vessels

250 20:00 20:00 N/A 500-1800 Off

Sample was clear, colorless with some particles remaining upon dilution to 50 ml.

Microwave Digestion ofCoal in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Coal Ash

Weigh 0.3 g of the sample into the digestion vessel. Add 3 ml of HNO3, 3 ml of HCl and 3 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

HNO3HClHF

15:00 15:00 800 900-1050 Off

Microwave Digestion ofCoal Ash

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Diesel Fuel

Weigh 0.25 g of the sample into the digestion vessel. Add 10 ml of HNO3 . Gently swirl the mixture and wait approximately 15 minutes topre-digest before closing the vessel.

The pre-digestion step will allow the lighter fuel fractions to react before sealing the vessels.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofDiesel Fuel

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Iron Ore

Weigh 1 g of the sample into the digestion vessel. Add 4 ml of H2O, 8 ml of HCl, 4 ml of HNO3, and 4 ml of HF. Gently swirl the mixture andwait approximately 15 minute before closing the vessel.

The addition of Conc. HCl (0-8 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. Theamount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit thetechniques or increase the difficulties of analysis.

MARS 6MARS 6 iWave

H2OHClHNO3HF

200 15:00 10:00 800 900-1050 Off

Microwave Digestion ofIron Ore

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Kerosene

Weigh 0.25 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes topre-digest before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofKerosene

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Limestone

Weigh 0.5 g of the sample into the digestion vessel. Add 7.5 ml of HNO3 and 2.5 ml of HF. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofLimestone

Step 1 of 2

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Procedure

Heating Program

Reagents

Method Note

Mill Tailings

Weigh 0.25 g of the sample into the digestion vessel. Add 3 ml of H3PO4, and 2 ml of H2SO4. Gently swirl the mixture before closing thevessel.

After heating program in step 1, allow vessel to cool before proceeding with step 2.

EasyPrep Plus vessels require a high temperature probe for this method.

MARS 6MARS 6 iWave

H3PO4H2SO4

270 15:00 30:00 800 900-1050 Off

Microwave Digestion ofMill Tailings (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Results

General Precautions

Mill Tailings

Cool, vent and open vessel after step 1. Add 2.5 ml HNO3, 2.5 ml HCl, 2.5 ml HF, and 2.5 ml H2O.

HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel.

The above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluoridesformed, an additional complexation step with boric acid should be used. This procedure can be found in method note entitled “Boric HFNeutralization”

HNO3HClHFH2O

200 15:00 20:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on yoursample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofMill Tailings (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Motor Oil (Waste)

Weigh 0.25 g (0.1 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes to pre-digest before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMotor Oil (Waste)

Step 1 of 2

www.cem.com

Procedure

Heating Program

Reagents

Method Note

Petroleum Coke

Weigh 0.1 g of the sample into the digestion vessel. Add 10 mL of H2SO4. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

Mars iWaveMars 6

0.1 g Inorganic Ramp to Temperature One Touch

260 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPetroleum Coke (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Results

General Precautions

Petroleum Coke

Proceeding step 1 add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPetroleum Coke (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Rock (pulverized)

Weigh 0.5 g of the sample into the digestion vessel. Add 5 mL of HNO3, 3 mL of HCl, and 3 mL of HF. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HClHFHNO3

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofRock (Pulverized)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Rutile Ore

Weigh 0.25 g of the sample into the digestion vessel. Add 4 ml of HNO3, 3 ml of H3PO4, and 8 ml of HF. Gently swirl the mixture beforeclosing the vessel.

MARS 6MARS 6 iWave

HNO3HFH3PO4

240 15:00 20:00 800 900-1050 Off

Microwave Digestion ofRutile Ore

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.5 g of the sample into the digestion vessel. Add 3 ml of HNO3, 2 ml of HCl, and 5 ml of HF. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HFHCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSand

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Silica Sand

Weigh 0.5 g of the sample into the digestion vessel. Add 5 ml of H2O, 3 ml of HNO3, and 8 ml of HF. Gently swirl the mixture before closingthe vessel.

Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

Reducing the particle size increase the efficiency of digestion and may decrease the digestion time.

MARS 6MARS 6 iWave

H2OHNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSilica Sand

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Silicon Dioxide

MARS 6MARS 6 iWave

HNO3HF

180 10:00 15:00 800 900-1050 Off

Microwave Digestion ofSilicon Dioxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Silicon Wafer

Weigh 0.5 g of the sample into the digestion vessel. Slowly add 3 ml of HNO3, and 6 ml of HF. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HF

180 10:00 15:00 800 900-1050 Off

Microwave Digestion ofSilicon Wafer

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Slag (Furnace)

MARS 6MARS 6 iWave

HNO3HClHF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSlag (Furnace)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Titanium Dioxide

MARS 6MARS 6 iWave

HNO3HF

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTitanium Dioxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tungsten Carbide

Weigh 0.25 g of the sample into the digestion vessel. Slowly add 5 ml of HNO3, and 10 ml of HF. Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

HNO3HF

220 20:00 20:00 800 900-1050 Off

Microwave Digestion ofTungsten Carbide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Tungsten Oxide

Weigh 0.5 g of the sample into the digestion vessel. Slowly add 2 ml of HNO3, and 7 ml of HF. Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

HNO3HF

220 20:00 20:00 800 900-1050 Off

Microwave Digestion ofTungsten Oxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Zeolite

MARS 6MARS 6 iWave

HNO3HF

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofZeolite

Industrial Hygiene

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter Paper (Cellulose)

Weigh 1 filter (approximately 0.9 g) of the sample into the digestion vessel. Add 5 ml of H2O, and 5 ml of HNO3. Gently swirl the mixture andwait approximately 15 minutes before closing the vessel.

Add H2O before HNO3.

MARS 6MARS 6 iWave

H2OHNO3

1 filter (approximately 0.9 g) Organic Ramp to Temperature One Touch

180 15:00 10:00 800 900-1050 Off

Microwave Digestion ofFilter Paper (Cellulose)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter (37 mm Mixed Cellulose Ester)

Weigh 1 filter (approximately 0.3 g) into the digestion vessel. Add 10 ml HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

XpressXpressPlusEasyPrepEasyPrep pLus

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFilter (37 mm Mixed Cellulose Ester)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter (47 mm Polycarbonate)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFilter (47 mm Polycarbonate)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ghost Wipe

Weigh 1 wipe (approx 2.5 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

Ensure that the entire wipe is covered with reagent.

MARS 6MARS 6 iWave

1 wipe (approx 2.5 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGhost Wipe

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Toray Filter in iPrep Vessels

Weigh 1 filter (approximately 0.3-0.5 g) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture before closingthe vessel.

1 filter(approximately 0.3-0.5 g)

Organic Ramp to Temperature One Touch

260 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofToray Filter in iPrep Vessels

Materials Science

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Circuit Board (cryo-ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 3 mL of HNO3 and 9 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

HCl should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

This method is considered a leach and may not provide a total digestion.

MARS 6MARS 6 iWave

HClHNO3

200 15:00 15:00 800 900-1050 Off

Sample was clear, with particles upon dilution to 50 mL.

Microwave Digestion ofCircuit Board (Cryo-ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Glass (ground)

Weigh 0.1 g of the sample into the digestion vessel. Add 2 mL of HNO3, 4 mL of HCl, and 3 mL of HF. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HClHFHNO3

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGlass (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Graphite Fiber (Epoxy Resin, Fiber Content)

Weigh 1 g of the sample into the digestion vessel. Add 30 ml of HNO3. Gently swirl the mixture before closing the vessel.

Sample must be completely covered with acid prior to digestion.

After digestion the samples are filtered and the graphite fibers are rinsed with deionized water.

This method is for the gravimetric (weight) determination of fiber content.

MARS 6MARS 6 iWave

150 10:00 10:00 800 900-1050 Off

Sample was digested, but the fiber filler material is left undigested for subsequent gravimetric analysis.

Microwave Digestion ofGraphite Fiber (Epoxy Resin, Fiber Content)

Metals and Alloys

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aluminum Alloy

Weigh 0.25 g of the sample into the digestion vessel. Add 5 ml of H2O and 5 ml of HCl .Gently swirl the mixture before closing the vessel.

Add H2O before HCl.

Add HCl slowly, and allow vessels to stand in the fume hood until initial reaction subsides.

MARS 6MARS 6 iWave

HClH2O

0.25g Organic Ramp to Temperature One Touch

190 10:00 15:00 800 900-1050 Off

Microwave Digestion ofAlluminum Alloy

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Alpha - Alumina in iPrep Vessels

Weigh 0.5 g of sample into the digestion vessel. Add 10 ml HCl. Gently swirl the vessel to thoroughly mix the sample and acid.

MARS 6 iWave iPrep HCl

240 30:00 60:00 N/A 1800 N/A

Microwave Digestion ofAlpha - Alumina in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aluminum Oxide

Weigh 0.25 g of sample into the digestion vessel. Add 6.5 ml H3PO4 and 3.5 ml H2SO4. Gently swirl the vessel to thoroughly mix thesample and acid.

MARS 6MARS 6 iWave

Easy PrepEasy Prep Plus

H3PO4H2SO4

270 30:00 20:00 N/A 1800 N/A

Microwave Digestion ofAluminum Oxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aluminum (metal)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HCl and 3 mL of DI H2O. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

HCl should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

MARS 6MARS 6 iWave

HClDI H2O

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAluminum (Metal)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Copper (metal)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on yoursample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature toavoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofCopper (Metal)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Copper (Ore)

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add10 ml of Aqua Regia (3:1 HCl:HNO3 ,). Gently swirl themixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

180 15:00 10:00 800 900-1050 Off

Sample was clear, and colorless with some remaining particles upon dilution to 50 ml.

Microwave Digestion ofCopper (Ore)

Step 1 of 2

www.cem.com

Procedure

Heating Program

Reagents

Method Note

Metal Alloy (CoCr)

Weigh 0.5 g of the sample into the digestion vessel. Add 5 ml of HNO3, 5 ml of HF, and 5 of ml H2O. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

The addition of Deionized Water may improve solubility of metal alloys.

MARS 6MARS 6 iWave

EasyPrepEasyPrepPlus

HNO3HFH2O

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMetal Alloy (CoCr) (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Results

General Precautions

Metal Alloy (CoCr)

Cool, vent and open vessel after step 1. Add 4 ml of H2O2, (30%)

200 10:00 10:00 800 900-1050 Off

Microwave Digestion ofMetal Alloy (CoCr) (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Metal Alloy (FeCr)

Weigh 0.25 g of the sample into the digestion vessel. Add 5 ml of HCl, 5 ml of HNO3, and 5 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

HClHFHNO3

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMetal Alloy (FeCr)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Metal Alloy (NiCr)

Weigh 0.5 g of the sample into the digestion vessel. Add 5 ml of H2O, 5 ml of HNO3, and 5 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

H2OHNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMetal Alloy (NiCr)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Nickel (metal)

Weigh 0.5 g of sample into the digestion vessel. Add 5 mL of HNO3 and 5 mL of DI H2O. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3DI H2O

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofNickel (Metal)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Platinum (Metal)

Weigh 0.5 g of the sample into the digestion vessel. Add 10 ml of Aqua Regia (3:1 HCl:HNO3). Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

HNO3HCl

180 15:00 20:00 800 900-1050 Off

Microwave Digestion ofPlatinum (Metal)

Step 1 of 2

www.cem.com

Procedure

Heating Program

Reagents

Method Note

Precious Metal (Ore)

Weigh 0.1 g of the sample into the digestion vessel. Add 10 ml of HNO3, and 0.5 ml of HF. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 20:00 800 900-1050 Off

Microwave Digestion ofPrecious Metal (Ore) (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Results

General Precautions

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on yoursample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or belowroom temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Precious Metal (Ore)

Cool, vent and open vessel after step 1. Add 5 ml of HCl.

180 10:00 10:00 800 900-1050 Off

Microwave Digestion ofPrecious Metal (Ore) (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Selenium Alloy

Weigh 1 g (0.5 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

Microwave Digestion ofSelenium Alloy

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

SnAg Solder

Weigh 0.5 g of the sample into the digestion vessel. Slowly add dropwise 10 ml of 1:1:1 H2O:HNO3:HF (premixed) to the sample. Gentlyswirl the mixture and wait approximately 15 minutes before closing the vessel.

Caution must be taken when adding the acid solution to the sample as the reaction is very vigorous and exothermic.

MARS 6MARS 6 iWave

H2OHNO3HF

180 15:00 10:00 800 900-1050 Off

Microwave Digestion ofSnAg Solder

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Stainless Steel

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HCl, 3 ml of HNO3, and 3 ml of HF. Gently swirl the mixture before closingthe vessel.

MARS 6MARS 6 iWave

HNO3HFHCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofStainless Steel

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Steel (Stainless)

This method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide completedigestion of some sample matrixes.

MARS 6MARS 6 iWave

HClHNO3

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSteel (Stainless)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Titanium Alloy

MARS 6MARS 6 iWave

HNO3HFHCl

200 15:00 30:00 800 900-1050 Off

Microwave Digestion ofTitanium Alloy

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Titanium Dioxide

MARS 6MARS 6 iWave

HNO3HF

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTitanium Dioxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Zinc Oxide

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofZinc Oxide

Nutraceuticals

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Biotin

Weigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofBiotin

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ginko (Ground)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGinko (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ginko (Whole Pill)

Weigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2O2

1 pill (Approx 1.0 g) Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGinko (Whole Pill)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Liquid)

Transfer 1 ml (0.5 ml w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofInfant Formula (Liquid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Liquid) in iPrep Vessels

Transfer 2 ml of of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

210 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofInfant Formula (Liquid) in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Powder)

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofInfant Formula (Powder)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Infant Formula (Powder) in iPrep Vessels

Weigh 1 g of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

210 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofInfant Formula (Powder) in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofKelp

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Milk (Liquid, Whole)

Transfer 4 ml of the liquid sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMilk (Liquid, Whole)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Milk (Powder)

MARS 6MARS 6 iWave

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofMilk (Powder)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Plant Tissue

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPlant Tissue

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWheat

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Whey Powder

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

a) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results onyour sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below roomtemperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofWhey Powder

Organic Chemicals

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Photoresist

Add 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPhotoresist

Paints and Coatings

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Paint (Latex Based Liquid)

Weigh 1 g (0.5 g w/Xpress Vessels) of sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture before closing thevessel.

HF is required for a total dissolution of inorganic filler if present.

If this procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed,an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “BoricAcid HF Neutralization”.

MARS 6MARS 6 iWave

200 15:00 10:00 800 900-1050 Off

Microwave Digestion ofPaint (Latex Based Liquid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Paint Chips (For Pb Analysis)

HF may be required for a total dissolution.

MARS 6MARS 6 iWave

200 15:00 10:00 800 900-1050 Off

Sample was clear, colorless, with some remaining inorganic particles upon dilution to 50 ml.

Microwave Digestion ofPaint Chips (For Pb Analysis)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Photoresist

Add 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPhotoresist

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Toner (Ink) in iPrep Vessels

240 20:00 20:00 N/A 500-1800 Off

Sample was clear, colorless with some particles remaining upon dilution to 50 ml.

Microwave Digestion ofToner (Ink) in iPrep Vessels

Personal Care (Health and Beauty)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

H3BO3 (4%)

170 15:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cosmetics (Liquid Foundation)

Weigh 1 g of the sample into the digestion vessel. Add 8 ml of HNO3 and 2 ml of HF. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HF

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCosmetics (Liquid, Foundation)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Eye Shadow

Weigh 0.25 g of the sample into the digestion vessel. Add 7 mL of HNO3 and 3 mL of HF. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HFHNO3

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofEye Shadow

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Shampoo (Dandruff)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofShampoo (Dandruff)

Pharmaceutical and Biotech

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Allergy Pill (Ground)

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAllergy Pill (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Allergy Pill (Whole Pill)

Weigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2 O2 (30%)

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAllergy Pill (Whole Pill)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Antioxidant (Ground)

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAntioxidant (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Antioxidant (Whole Pill)

Weigh 1 pill (approx 1.4 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2 (30%). Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2O2 (30%)

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAntioxidant (Whole Pill)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

APIs with Aromatic Ring Structures in iPrep Vessels

Weigh 0.1 g - 0.5 g of the sample into the digestion vessel. Add 9 ml of HNO3 and 1 ml of HCl. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6 iWave iPrep HNO3HCl

0.1 g - 0.5 g Organic Ramp to Temperature One Touch

250 30:00 25:00 N/A 1800 N/A

Sample was clear, colorless, and particle free upon dilution to 50 ml. If the sample contains precious metals the diluted sample may be thecolor associated with the metals.

Microwave Digestion ofAPIs with Aromatic Ring Structures in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ascorbic Acid

MARS 6MARS 6 iWave

EasyPrepEasyPrep PlusXpress PlusXpress

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofAscorbic Acid

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aspirin (Ground)

Weigh 0.25 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAspirin (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Aspirin (Whole)

Weigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAspirin (Whole)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Beta Carotene

MARS 6 iWaveMARS 6

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofBeta Carotene

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Biotin

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofBiotin

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Calcium Carbonate

Weigh 0.5 g of the sample into the digestion vessel. Slowly add 9 ml of HNO3, and 1 ml of HCl. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

Allow initial reaction to subside before sealing vessel.

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofCalcium Carbonate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Calpan Pantothenic Acid

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCalpan Pantothenic Acid

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cephalexin

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCephalexin

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Chromium Chelate

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChromium Chelate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cold and Flu Medicine (Liquid)

Add 0.75 mL of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL of HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCold and Flu Medicine (Liquid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Cupric Sulfate

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Sample was clear, blue in color, and particle free upon dilution to 50 ml.

Microwave Digestion ofCupric Sulfate

Procedure

Heating Program

Reagents

www.cem.com Form preparedby:

MARS 6TM

Method Note

Results

General Precautions

Diclofenac K

MARS 6MARS 6 iWave

HNO3H2O2 (30%)

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofDiclofenac K

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Empty Capsule (gel)

Weigh 1 capsule (Approx 0.1 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

1 capsule (Approx 0.1 g) Organic Ramp to Temperature One Touch

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofEmpty Capsule (Gel)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Fish Oil (No Capsule)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFish Oil (No Capsule)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Folic Acid

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFolic Acid

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ginko (Ground)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGinko (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Ginko (Whole Pill)

Weigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of H2O2. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2O2

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGinko (Whole Pill)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofKelp

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Losataran K

MARS 6MARS 6 iWave

HNO3H2O2 (30%)

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofLosataran K

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Magnesium Carbonate

Allow any reaction to subside before sealing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofMagnesium Carbonate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Magnesium Oxide

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMagnesium Oxide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Manganese Carbonate

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofManganese Carbonate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Metaformin (ground)

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMetaformin (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Multivitamin (Ground)

Weigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO3 and 1 mL HCl. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMultivitamin (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Multivitamin (Whole)

Weigh 1 pill (Approx 2.5 g) into the digestion vessel. Add 10 mL of HNO3 and 2 mL of HCl. Gently swirl the mixture and wait approximately15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMultivitamin (Whole)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Niacinamide

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofNiacinamide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pantothenic Acid

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofPantothenic Acid

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Potassium Chelate

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofPotassium Chelate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Pyridoxine

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofPyridoxine

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Riboflavin

MARS 6MARS 6 iWave

HNO3HCl

230 20:00 30:00 800 900-1050 Off

Microwave Digestion ofRiboflavin

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Selenium Chelate

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofSelenium Chelate

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Stearic Acid

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofStearic Acid

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Thiamine

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofThiamine

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin B-12

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofVitamin B-12

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin C (ground)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofVitamin C (Ground)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin C (whole)

Weigh 1 pill (Approx 1.4 g) into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofVitamin C (Whole)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin D

MARS 6MARS 6 iWave

HNO3HCl

210 20:00 15:00 800 900-1050 Off

Microwave Digestion ofVitamin D

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin D3

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofVitamin D3

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamin E

MARS 6MARS 6 iWave

HNO3HCl

230 20:00 30:00 800 900-1050 Off

Microwave Digestion ofVitamin E

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Vitamins

Weigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 9 ml of HNO3, 1 ml HCl, and 1 ml of HF. Gently swirl themixture and wait approximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3HFHCl

210 15:00 30:00 800 900-1050 Off

Microwave Digestion ofVitamins

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Zinc Sulfate

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofZinc Sulfate

Plastics, Polymers, and Oils

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Acrylamide

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofAcrylamide

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Bunker Oil

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofBunker Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Bunker Oil in iPrep Vessels

250 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofBunker Oil in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Char (Sulfuric Acid)

Add 5 ml H2SO4 into the vessel that contains the sample and acid.

This method is for the pretreatment of large sample sizes or difficult organic samples that are resistant to oxidation. After the char iscomplete, the vessel is opened and a normal oxidation with HNO3 can be run, usually at around 200C.

MARS 6MARS 6 iWave

260 15:00 15:00 800 900-1050 Off

Microwave Digestion ofChar (Sulfuric Acid)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Crude Oil

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofCrude Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Diesel Fuel

Weigh 0.25 g of the sample into the digestion vessel. Add 10 ml of HNO3 . Gently swirl the mixture and wait approximately 15 minutes topre-digest before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofDiesel Fuel

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Epoxy Hardener

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofEpoxy Hardener

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Fatty Alcohol

MARS 6MARS 6 iWave

HNO3HCl

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFatty Alcohol

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Gasoline

Add 0.1g of the sample into the digestion vessel. Slowly add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutesbefore closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofGasoline

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

HDPE (High Density Polyethylene)

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofHDPE (High Density Polyethylene)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Kerosene

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofKerosene

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Kevlar in iPrep Vessels

Ensure that all fibers are completely covered with acid and not adhered to the vessel liner wall.

250 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofKevlar in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Lube Oil

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofLube Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Mineral Oil

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMineral Oil

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Motor Oil (Waste)

Weigh 0.25 g (0.1 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture and waitapproximately 15 minutes to pre-digest before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofMotor Oil (Waste)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Motor Oil (Waste) in iPrep Vessels

240 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofMotor Oil (Waste) in iPrep Vessels

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofNylon

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Weigh 0.2 g of the sample into the digestion vessel. Add 8 mL of HNO3 and 2 mL of H2SO4. Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

HNO3H2SO4

230 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPET (Polyethylene Terephthalate)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

PET (Polyethylene Terephthalate) in iPrep Vessels

250 20:00 20:00 N/A 500-1800 Off

Microwave Digestion ofPET (Polyethylene Terephthalate) in iPrep Vessels

Step 1 of 2

www.cem.com

Procedure

Heating Program

Reagents

Method Note

Polycarbonate Resin

Char Step

Weigh 1.0 g of the sample into the digestion vessel. Add 6 mL of H2SO4. Gently swirl the mixture and wait approximately 15 minutes beforeclosing the vessel.

MARS 6MARS iWave

260 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPolycarbonate Resin (Step 1 of 2)

Procedure

Step 2 of 2

Heating Program

Reagents

MARS 6TM

Results

General Precautions

Polycarbonate Resin

Proceeding step one, add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPolycarbonate Resin (Step 2 of 2)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Polyethersulfone (Filter Paper)

Weigh 1 filter paper (Approx 0.1 g) into the digestion vessel. Add 8 mL of HNO3 and 2 mL H2SO4. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2SO4

1 Filter (Approx 0.1 g) Organic Ramp to Temperature One Touch

230 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPolyethersulfone (Filter Paper)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Polypropylene

Weigh 0.25 (0.1 g w/Xpress vessels) of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and waitapproximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

0.25 g (0.1 g w/Xpress) Organic Ramp to Temperature One Touch

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPolypropylene

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Polyurethane

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPolyurethane

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

PVC (Polyvinyl Chloride, Resin)

Weigh 0.25 g (0.1 g w/Xpress Vessels) of sample into the digestion vessel. Add 10 ml of HNO3. Gently swirl the mixture before closing thevessel.

MARS 6MARS 6 iWave

210 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPVC (Polyvinyl Chloride, Resin)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Rubber

MARS 6MARS 6 iWave

220 15:00 15:00 800 900-1050 Off

Microwave Digestion ofRubber

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Terephthalic Acid

MARS 6MARS 6 iWave

230 15:00 15:00 800 900-1050 Off

Microwave Digestion ofTerephthalic Acid

Pulp and Paper

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter Paper (Cellulose)

Weigh 1 filter (approximately 0.9 g) of the sample into the digestion vessel. Add 5 ml of H2O, and 5 ml of HNO3. Gently swirl the mixture andwait approximately 15 minutes before closing the vessel.

Add H2O before HNO3.

MARS 6MARS 6 iWave

H2OHNO3

180 15:00 10:00 800 900-1050 Off

Microwave Digestion ofFilter Paper (Cellulose)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter (37 mm Mixed Cellulose Ester)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFilter (37 mm Mixed Cellulose Ester)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Filter (47 mm Polycarbonate)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofFilter (47 mm Polycarbonate)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Paper Pulp

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofPaper Pulp

Water and Wastewater Treatment

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

NPDES (Waste Water)

Transfer 50 ml of the sample into the digestion vessel. Add 3 ml of HNO3, and 2 ml HCl. Gently swirl the mixture before closing the vessel.

MARS 6MARS 6 iWave

Xpress (75 ml)Xpress PlusEasyPrepEasyPrep Plus

HNO3HCl

50 ml Water Ramp to Temperature One Touch

165 30:00 0:00 800 900-1050 Off

Microwave Digestion ofNPDES (Wastewater)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sludge (Industrial)

MARS 6MARS 6 iWave

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSludge (Industrial)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Sludge (Waste Activated)

Weigh 0.5 g dry weight (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 5 ml of HNO3, and 5 ml of H2O2. Gently swirlthe mixture and wait approximately 15 minutes before closing the vessel.

MARS 6MARS 6 iWave

HNO3H2O2

200 15:00 15:00 800 900-1050 Off

Microwave Digestion ofSludge (Waste Activated)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

TCLP Extract

If a high organic content is suspected, a 10 ml sample volume is recommended.

MARS 6MARS 6 iWave

EasyPrep PlusXpress (75 ml)Xpress Plus

25 mL EPA Digestion (Water) Standard Control One Touch

170 10:00 10:00 800 900-1050 Off

Microwave Digestion ofTCLP Extract

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3015 (Aqueous Samples)

MARS 6MARS 6 iWave

170 10:00 10:00 800 900-1050 Off

Microwave Digestion ofUS EPA 3015 (Aqueous Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

US EPA 3015a (Aqueous Samples)

Transfer 45 ml of the sample into the digestion vessel. Add 5 ml of HNO3, or alternatively 4 ml of HNO3 and 1 ml HCl. Gently swirl themixture before closing the vessel.

MARS 6MARS 6 iWave

HNO3HCl (Optional)

170 10:00 10:00 800 900-1050 Off

Microwave Digestion ofUS EPA 3015a (Aqueous Samples)

Procedure

Heating Program

Reagents

MARS 6TM

Method Note

Results

General Precautions

Water (for analysis of Phosphorus)

Weigh 50 mL of H2O into the digestion vessel. Add 0.5 g of K2S2O8 and 1 mL of H2SO4. Gently swirl the mixture and wait approximately 15minutes before closing the vessel.

MARS 6 XpressXpressPlusEasyPrepEasyPrep Plus

K2S2O8H2SO4

170 15:00 15:00 800 900-1050 Off

Microwave Digestion ofWater (for Analysis of Phosphorus)

Microwave Acid Digestion Method Note...

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Transcript of Microwave Acid Digestion Method Note...

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