Post on 24-Mar-2018
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Development of a short-term assay based on the evaluation of the plasma
membrane integrity of the alga Pseudokirchneriella subcapitata
(Supplementary Information - Applied Microbiology and Biotechnology)
Manuela D. Machado and Eduardo V. Soares
Author for correspondence:
Eduardo V. Soares
Address: Bioengineering
Laboratory, Chemical Engineering Department, Superior
Institute of Engineering of Porto Polytechnic Institute, Rua Dr António
Bernardino de Almeida, 431, 4200-072 Porto, Portugal;
e-mail: evs@isep.ipp.pt
Tel: 351-22-8340500
Fax: 351-22-8321159.
Fig. S1. Visualization of heat
Green. In order to permeabilize the plasma membrane, a
(65ºC, 1h) and subsequently incubated with 0.5 μmol/l SYTOX Green for 40 minutes at
25°C. Fluorescence micrograph (a); phase contrast micrograph of the s
Visualization of heat-treated cells of the alga P. subcapitata stained with SYTOX
In order to permeabilize the plasma membrane, algal cells were heat
subsequently incubated with 0.5 μmol/l SYTOX Green for 40 minutes at
Fluorescence micrograph (a); phase contrast micrograph of the same cells (b).
2
stained with SYTOX
lgal cells were heat-treated
subsequently incubated with 0.5 μmol/l SYTOX Green for 40 minutes at
ame cells (b).
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Fig. S2. The fluorescence response to increasing numbers of P. subcapitata cells
incubated with SYTOX Green. Live cells (open circles) and dead cells (closed circles)
were incubated with 0.5 μmol/l SYTOX Green for 40 minutes at 25°C. Each point
represents the mean of five fluorescence readings; standard deviations are presented
with 95% confidence limits (vertical error bars).
0
20
40
60
80
100
120
140
160
0 2 4 6 8 10
Flu
ore
scen
ce (
RF
U x
10
3)
Cell density (cells x 105/ml)