Post on 13-Feb-2022
3 © 2014 3M. All Rights Reserved.
Enabling Customer Success:Our Five Market-Leading Businesses Groups
> Sales: $29.9 B
> R&D investment $1.6 B
> 3,100+ patents issued in
2012
> 40,500+ issued & pending
patents
Industrial
From purification to aerospace –
changing how industry works
Health Care
From preventing infections to making smiles
brighter
Safety & Graphics
From protecting people & information to
enhancing visual & design communication
Consumer
From simplifying life at home to keeping
you organized at work
Electronics & Energy
Enabling tomorrow’s lifestyle today with power,
communications and electronics
4 © 2014 3M. All Rights Reserved.
FsFiltration,
Separation, Purification
ImImaging
LmLight
Management
EcEnergy
Components
OpOpto-electronics
TtTrack and Trace
AcAcoustic Control
DiDisplay
MfMechanical Fasteners
MdMedical Data Management
FeFlexible
Electronics
WoWound
Management
MiMicrobial Detection
and Control
DdDrug
Delivery
BiBiotech
Applications
PrProcess Design &
Control
SeSensors
WeAccelerated Weathering
InInspection & Measurement
IsIntegrated
Systems & Design
FcFlexible Converting
& Packaging
AnAnalytical
AsApplicationSoftware
EsElectronics &
Software
Capabilities
RpRadiation
Processing
SuSurface
Modification
VpVapor
Processing
PePredictive
Engineering & Modeling
PmPolymer
Processing
PpPrecision
Processing
MoMolding
MrMicro-replication
PdParticle & Dispersion Processing
Processing
FlFluoro-materials
NtNano-
technology
AbAbrasives
AdAdhesives
AmAdvancedMaterials
DoDental &
Orthodontic Materials
FiFilms
NwNonwovenMaterials
PoPorous Materials &
Membranes
SmSpecialty Materials
CeCeramics
EmElectronicMaterials
CoAdvanced
Composites
Materials
46 Core Technology PlatformsSuccessful New Product Growth Builds on Uncommon Connections
5 © 2014 3M. All Rights Reserved.
3M Innovation Model
Abrasives
Adhesives
Ceramics
Electronics, Software
Films
Microreplication
Nanotechnology
Non-wovens
Precision Coating
Vapor Processing
Architecture & Construction
Consumer & Office
Display
Electronics
Filtration
Health Care
Renewable Energy
Safety & Security
Telecoms and Utilities
Water Infrastructure
Doing Things Only 3M Can Do
Technology Platforms Markets
Opportunity
Drives
Development
and Utilization
6 © 2014 3M. All Rights Reserved.
A Century of Innovation
1921
3M patented and introduced WetordryTM
waterproof sandpaper -- the world’s first water-resistant coated abrasive 1995
3M introduced the first metered dose asthma inhaler, free of ozone depleting chlorofluorocarbons.
1979Thinsulate™ thermal
insulation introduced
1939
First traffic sign featuring Scotchlite™ reflective sheeting erected in Minneapolis
1991
3M introduced
Scotchshield™ window film,
shatter-resistant, heat-and cold
resistant window protection.
1985
Academy of Motion Picture Arts and
Sciences gave 3M a Scientific
Engineering Award for magnetic film,
improving audio capabilities of movie
sound tracks
1945Scotch TM vinyl electrical
tape introduced
1952
Scotchlok™ electrical connectors and Scotchkote™ insulation introduced
1947
Scotch TM magnetic audiotape introduced
3M’s first
non-woven
product-
decorative
ribbon for
gifts
introduced
1954
RCA used ScotchTM magnetic tape to record TV programs for the first time
1960
ScotchTM Brand Magic transparent tape introduced
1969
3M products were used in the first moon walk on July 20. Astronaut Neil Armstrong left a footprint in the lunar dust in boots made from Fluorel™ synthetic rubber from 3M
19743M introduced Post-it® notes, greatly enhancing office communication
1967
3M developed the first disposable facemasks and respiratory protection products
1931
3M began producing Colorquartz™ roofing granules
1925
ScotchTM
masking tape introduced
1996
New products include flexible circuits for electronic products and HFEs (hydrofluoroethers), replacing ozone depleting chlorofluorocarbons 2000
3M introduced the Vikuiti™ brand for
light management products that make
electronic displays easier to read
20053M developed Aluminum Conductor Composite Reinforced (ACCR) as a solution to thermally constrained transmission bottlenecks
3M debuted
its first
surgical
drape
1927
ScotchTM Cellulose tape introduced
2010
3MTM CubitronTM II using precision shaped abrasive granules creating the future of the abrasives industry
20093MTM MPro pocket-sized projector for mobile presentations and sharing photos on the go
1948
20123M LED advanced
light, most
energy efficient,
longest-lasting
light bulb
7 © 2014 3M. All Rights Reserved.
Yeast & Mold Testing Yeasts & Molds are an important quality indicator in the food and
beverage industry, impacting spoilage and shelf life
― one of the top four indicator organisms
― Estimated to be 14% of total indicator testing volume
Important food segments for Yeast & Mold:
― Cultured dairy, fruits & vegetables, bakery products,
confectionary, RTE meats, nuts, spices, dry goods
Characteristics of Yeast & Molds:
― Grows well in low pH and low water activity
― Requires long incubation time: 5 days (reference method) vs.
most indicator organisms are incubated 24-48 hrs.
― Difficult to interpret (count): Molds grow as a irregular spreading
mass (as opposed to a pinpoint colony like bacteria)
Total Ct.34%
Coliform15%
E. coli 15%
Yeast and Mold14%
Staph aureus6%
EB6%
Other10%
Indicator Tests Worldwide
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Reference methods for Yeast & Mold testingFDA-BAM Reference Methods ISO Reference Methods
FDA-BAM, Chapter 18
Spread-plate or Pour-plate
DRBC, DG18 or PDA, 25oC, 5-7 days*
0.1% Peptone water
25-50 g sample, 11 mL/99 mL (Dairy)
Dairy (SMEDP)
Spread-plate
Yeast extract glucose chloramphenicol agar,
PDA or DRBC, 25oC, 5 days*
Butterfield’s phosphate buffered water
ISO 21527-1 -2008 (Aw >0.95)
Spread-plate
Dichloran rose bengal chloramphenicol agar (DRBC)
0.1% Peptone water, 25oC, 5 days
ISO 21527-2 -2008 (Aw <0.95)
Spread-plate
Dichloran 18% glycerol agar (DG18)
0.1% Peptone water, 25oC, 5-7 days
ISO 6611: 2004 (Dairy)
Pour-plate
Yeast extract glucose chloramphenicol agar
25oC, 5 days
* Count plates after 5 days of incubation. If there is no growth at 5 days, re-incubate for another 48 h
9 © 2014 3M. All Rights Reserved.
Comparison of Techniques
Prepare Sample
Prepare Agar
Pour plates, allow to solidify
1st Dilution:
• Dispense 1mL over
3 plates
• Spread inoculum
on each plate0.3mL 0.3mL 0.4mL
0.1mL
3rd Dilution:
• Make 1:10 dilution of
the first dilution
• Dispense and spread
0.1mL over one plate
Incubate
0.1mL
Prepare Sample
Prepare Agar
1st Dilution:
Inoculate empty
dish
Pour agar, swirl,
allow to solidify
2st Dilution:
Make further 1:10
dilution, inoculate
1mL, pour agar,
allow to solidify
1.0 mL
Incubate
3rd Dilution:
Make further 1:10
dilution, inoculate
1mL, pour agar,
allow to solidify
1.0 mL
1.0 mL
Prepare Sample
1st Dilution:
Inoculate 3M
Petrifilm Plate
2st Dilution:
Make further 1:10
dilution, inoculate
1mL, on 3M
Petrifilm Plate
Incubate
25
3rd Dilution:
Make further 1:10
dilution, inoculate
1mL, pour agar
Spread Plate Process Pour Plate Process 3M Petrifilm Plate Process
2525
2nd Dilution:
• Dispense 0.1mL of first
dilution on one further
plate
• Spread inoculum
10 © 2014 3M. All Rights Reserved.
Yeast & Mould’s usually take much longer to grow and be counted than other microbiology tests
The group are very different from other microbes we count in traditional microbiology testing
Eukaryotic cells as compared to bacteria that are Prokaryotic
Very different from Bacteria including size
Often have slower metabolic pathways
Utilise different growing conditions
― Aerobic
― Can grow in lower Water Activity than bacteria
― Lower pH
― Often produce antibiotics so can be difficult competitors
Why does Yeast & Mould testing take so long?
13 © 2014 3M. All Rights Reserved.
Total Viable Count
― 48 hrs with most food matrices
― Some newer tests allow this result within 24 hrs
E.coli Testing
― 24 hrs
Enterobactericeae testing
― 24 hrs with VRBGA or Petrifilm
Yeast & Mould Testing
― 5 days
Times of some common Food microbiology tests
14 © 2014 3M. All Rights Reserved.
Food testing for Yeast & Mould contamination is normally relate to food spoilage and
not food poisoning
Only a few relevant mycology food poisoning cases are seen every year
Commercially it has a huge impact on the value of food and the amount of food
wasted globally
Mould contamination can be a very visual issue
Yeast contamination often causes taste changes in a food sample
Food testing for Yeast & Mould contamination
15 © 2014 3M. All Rights Reserved.
As Yeast & Moulds are a very large group we often get mixed
colonies on a plate
These can overlap and make reading very difficult
In some cases a 3 day and a 5 day read are needed
Difficult to read
16 © 2014 3M. All Rights Reserved.
Opening an agar plate can lead to contamination of your lab with mould spores
For weeks after none selective media can be contaminated with small mould colonies
This can result in the need for cleaning with harsh chemicals such as Phenols
In severe cases the lab can need to be fumigated
Adds a lot of inconvenience and expense in the lab
Laboratory Contamination
17 © 2014 3M. All Rights Reserved.
Description: 3M Petrifilm Yeast & Mold plate with significantly improved time-to-result and interpretation.
Rapid Petrifilm™ Yeast & Mold Plate – Product Summary
Image of plate post-incubation:
colonies indicated by blue color
Key Features & Benefits:
• Yeast & Mold Plate with 48-60 hour incubation time across a wide variety of foods.
• AOAC RI and ISO-AFNOR validated results
• Easier interpretation on Yeast & Mold plate(special technology that keeps mold colonies from spreading out and overlapping)
• New indicator dye technology colors colonies blue and make them appear faster.
• Foam dam added to plate for easier inoculation
• All of the traditional benefits of Petrifilm plates are maintained: simplicity, ease-of-use, shelf life, consistency, eliminates agar prep.
18 © 2014 3M. All Rights Reserved.
RY&M Petrifilm Advantages
― Shorter incubation times up to 50% faster
― Easier interpretation less time to read the plates
― Single media use High and low water activity on single plates
― Ready to use. No wait to use the product
― Consistent (Especially important with the addition of antibiotic
supplements. When this is missed out the results given are not
accurate)
― Long shelf life 18 months compared to 3-4 weeks
― Smaller footprint 90% space saving
― Less waste 90% less material to autoclave / pay to dispose
― Sealed plates Reduce the risk of laboratory contamination
Comparison between traditional agar & Rapid Y&M plates