Post on 31-Mar-2015
AP Lab 7Wisconsin Fast Plants“Who’s The Father?
What are Wisconsin Fast Plants?Read the lab and do p.2
• Type of mustard, radish, cabbage plant especially bred by Paul Williams of the University of Wisconsin
WFP Life Cycle Video
• Fast Plants Time Lapse Video
See stems and ANL (purple) and anl (non-purple or green)
Grow seeds in Petri dishUse Lid as bottom
Other part as cover Cut paper towel circle• Wet paper towel well with
a pipette
NAME
Water is to break the seed coat
Germinates in a day or two
FRIDAY
-Mark Petri dish lid into two halves
-Label P on one side, F1 the other
-Moisten paper towel circle and place 5 seeds in each side of the lid
F2 WFP (1:57)
P F1Put you initials on the Petri dish cover
Root emerges with root hairs to increase surface area for water absorption.
Petri dish at slight angle under a light
It will sit all week-end so be sure there is enough water in the Petri dish.
Day4 (MONDAY)(cont. on next slide)
• Stem and leaves emerge• Water F1 and P well.
• “Observing the Seeds” (make 3 quantitative and 3 qualitative observations, sketch and color, count the genotypes and phenotypes.)
• Attach a paper with these observations.
Monday: Now plant F2
• Place 10 seeds in a Petri dish• Two rows of 5• Water well.• Label F2
• Cover• Label your initials• Put under light at
a slight angle
0 0
0 0
0 0
0 0
0 0
Tuesday
• Take data for F1 and P
• Check on seeds –add water if needed
Day 7 (Friday)
• DATA COLLECTION DAY OF F2
Purple/StandardNon-Purple/StandardPurple/Rosette-DwarfNon-Purple/ Rosette Dwarf
(see pictures next slide)
Friday: Count F2
Rosette dwarf
Purple/standard stemNon-purple/Standard stem
Collect Group Data
• P. 6• Sketch plants, color• Do dihybrid of the plant genotypes
Chi square Test
• Bozeman Biology Chi Square Test• To test your null hypothesis• Critical value and degrees of freedom
Day14-20 (flowers)
How do you grow WFP?
•Need constant light
How do you grow WFP?
•Seeds are tiny-do not cover with very much potting soil
How do you grow WFP?
•Need fertilizer
Label your quads
Green and Non-green leaves
• Wisconsin Fast Plants® F1 Non-Purple Stem, Yellow-Green Leaf (F1 Anthocyaninless, Yellow-Green)
• F1 seed from cross of Non-Purple Stem, Yellow-Green Leaf (anl/ygr) × Purple Stem (ANL/YGR);