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Sample pretreatment -------------------------- 144
Column protection----------------------- 145~147
Tubing parts ------------------------------ 148~151
Empty columns and other parts ----- 152~155
Pressure resistant empty glass columns -- 156, 157
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Excellent recovery
SampleYMC Dispo
SPECartridge,
Company ACartridge,
Company B
Basic sample (Propranolol hydrochloride) 89(99)% 59(73)% 66(89)%
Acidic sample (Thioctic acid) 91(100)% 92(89)% 87(88)%
*Figures inside parentheses represent recoveries obtained when buffer was used.
YMC Dispo SPE C18
Product No. Specification
XQSPEC18101 C18 100mg/1mL 100-pack
XQSPEC18203 C18 200mg/3mL 50-pack
XQSPEC18503 C18 500mg/3mL 50-pack
XQSPEC18A06 C18 1000mg/6mL 30-pack
YMC Dispo SPE SIL
Product No. Specification
XQSPESIL503 SIL 500mg/3mL 50-pack
XQSPESILA06 SIL 1000mg/6mL 30-pack
Material properties of products
Sample pretreatment
Sample pretreatment, Accessories
Stainless (SUS-316)
A general-purpose material for general HPLC products.
Contains Ni, Cr, Mo, etc, as well as Fe, and is highly resistant
to corrosion.
PEEK (polyetheretherketone)
A super engineering plastic developed as an alternative to
metals.
A polymer containing ketones and ether bonds, it is highly
resistant to pressure, chemicals, heat, and fatigue. Used as
an alternative to stainless for a metal-free tubing system.
Teflon (polytetrafluoroethylene, PTFE)
A polymer of tetrafluoroethylene. Highly resistant to
chemicals but not so resistant to pressure as PEEK.
Generally used for low pressure tubing systems.
(Teflon is a trademark of DuPont.)
Polyacetal
An engineering plastic. A polymer in which aldehyde
compounds are polymerized, with excellent chemical
resistance and excellent machinability.
Polypropylene
A polymer of propylene. Excellent chemical and heat
resistance. Often used for containers since processing,
such as injection molding, is easy.
Sample pretreatment cartridge
YMC Dispo SPE
Disposable type cartridge made from polypropylene Utilizes packing material designed for HPLC columns,
manufactured under strict quality controls Excellent cartridge-to-cartridge reproducibility High recovery of samples
ContainerMade from medical-grade polypropylenewith minimal elution of additives.
Upper and lower fritsPore size of 20 µm
Luer tipCan be connected to commerciallyavailable vacuum solid phaseextraction systems.
Solid phaseYMC*GEL
Vacuum
Syringe
Vacuum method Compression method Centrifugation
YM
C D
ispo SP
E
YM
C D
ispo SP
E
Adapter
YMC Dispo SPE
Vacuum method is suitable for large-scale treatment and compression method is suitable for small-scale treatment
Method of use
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Stainless steel precolumn filter
Product No. Specification
XRPRCS02 For tubing system with 1/16” outer diameter, 1 filter included
XRPRCS25 5 spare filters 2 μm are included
PEEK precolumn filter
Product No. Specification
XRPRCP02 For tubing systems with 1/16” outer diameter, 1 filter included
XRPRCP25 5 spare filters 2 μm are included
Column protection
Column : YMC-Pack ODS-A (5 μm, 120 Å) 150 X 4.6 mmI.D.
Eluent : acetonitrile/water (60/40)Flow rate : 1.0 mL/minTemperature : 37°CDetection : UV at 270 nm, 0.32 AUFSSample : 1. Uracil (0.05 mg/mL)
2. Methyl benzoate (0.5 μL/mL) 3. Butyl benzoate (1.5 μL/mL) 4. Butylbenzene (12.0 μL/mL)
Stainless steel precolumn filters are convenient for the case shown below: Problem prevention
One of the causes of a gradual increase of a column pressure after eachsample injection is that fine particles in the sample clog the frit of thecolumn. Replacing the column frit is one solution; however it is moreconvenient to use precolumn filter. Connect a precolumn filter betweenthe injector and the column, check the line pressure occasionally, andreplace the precolumn filter when the line pressure increases.Stainless steel precolumn filters are suitable for stainless steel tubingsystems. Both PEEK and stainless steel precolumn filters are suitable forPEEK tubing systems.
High-molecular weight compounds (proteins, DNA, sugars,etc.) that easily and irreversibly adsorb to the main columnare likely to cause a pressure increase and separationfailure. When these compounds adsorb to the main column,it is difficult to recover column performance by cleaning.A guard column will protect the main column when foulingof the main column is due to contamination from samplematrices.
YMC guard cartridges
YMC-Pack
Precolumn �lter
Stainless steel precolumn filter
Filter construction with minimal dead volume Effective diameter of the filter is extremely small about 1.6 mm.
Sample diffusion that occurs in passage is minimized.
PEEK precolumn filter
Metal free The material of the wetted part of the holder is PEEK and the packing is PCTFE.
The packaging that of the filter is a mixture of PEEK and Teflon. PEEK precolumn
filters are suitable for use in HPLC systems requiring a metal-free environment.
High-pressure resistance PEEK precolumn filters are resistant to ca. 35 MPa.
Retainer
Filter
1 23
4
10 20 min0Theoretical plate number (peak 4) : Pressure (MPa) :
11,200±200 (n=3)5.4±0 (n=3)
12
34
10 20 min0Theoretical plate number (peak 4) : Pressure (MPa) :
11,500±300 (n=3)5.4±0 (n=3)
Main column only
There is no decrease of column efficiency due to connection of a precolumn filter.
After connection of
a precolumn filter
Filter
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YMC guard cartridges
Wide variety of packing materials YMC*GEL with various functional groups identical to those
available for analytical columns is packed under high pressure.
Disposable The cartridge holder can be used repeatedly. Only the cartridge
column needs replacement when worn out.
Easy cartridge replacement The main body of the YMC guard cartridge is composed of a
cartridge holder and a cartridge column. The cartridge column
is threaded for easy replacement.
cartridge column
column coupler
cartridge holder
Guard cartridge column for semi-preparative
Pressure resistance up to 30 MPa (4350 psi) Ideal for protecting semi-preparative column
cartridge holder
cartridge column
Cartridge Column
Column sizeinner diameter X length (mm)
Product number1/pack 5/pack
1.0 X 10 -0101GC-1 -0101GC2.1 X 10 -01Q1GC-1 -01Q1GC3.0 X 10 -0103GC-1 -0103GC4.0 X 10 -0104GC-1 -0104GC1.0 X 20 -0201GC-1 -0201GC2.1 X 20 -02Q1GC-1 -02Q1GC3.0 X 20 -0203GC-1 -0203GC4.0 X 20 -0204GC-1 -0204GC
A cartridge holder will need to be purchased separately before using this product for the first time.
Cartridge Holder Set
cartridge holder set(10 mm length) XPGCH-Q1
cartridge holder set(20 mm length) XPGCH-Q2
Cartridge holder set includes a cartridge holder (1 set), and a column coupler.
Cartridge Column (2/pack)
Column sizeinner diameter X length (mm)
Product number
10 × 10 -0110CC20 × 20 -0120CC30 × 20 -0130CC
A cartridge holder will need to be purchased separately before using this product for the first time.
Cartridge Holder
cartridge holder (inner diameter : 10 mm) XPCHSPW1cartridge holder (inner diameter : 20 mm) XPCHSPW2cartridge holder (inner diameter : 30 mm) XPCHSPW3
* Please refer to the page of each product for product number.Example) Triart C18 (5 μm, 120 Å) = TA12S05
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EXP®Cartridge Column (Max. pressure: 100 MPa, 3/pack)
Particle sizeColumn size
inner diameter X length (mm)
Product number
Triart C18 Triart C8 ExRS Triart C8 Triart Phenyl Triart PFP
1.9 μm2.1 X 5 TA12SP9-E5Q1CC TAR08SP9-E5Q1CC TO12SP9-E5Q1CC TPH12SP9-E5Q1CC TPF12SP9-E5Q1CC
3.0 X 5 TA12SP9-E503CC TAR08SP9-E503CC TO12SP9-E503CC TPH12SP9-E503CC TPF12SP9-E503CC
EXP®Guard Cartridge Holder (Adjustable ferrule, Max. pressure: 137 MPa)
EXP®direct connect holder (for both 2.1 mm and 3.0 mm inner diameter, 2 titanium hybrid ferrule and 1 nut included)
XPCHUHP
Fitting for Connecting System and Main Column/Guard Cartridge (Adjustable ferrule, Max. pressure: 137 MPa)
Hand-tight EXP® fitting (Hand-tight nut and titanium hybrid ferrule included) XRHTF-01
Fitting for connecting system and main column/guard cartridge is also available.
Replacement Ferrule
Titanium hybrid ferrule (5/pack) XRHTF-05
Guard cartridge column for UHPLC
�High Pressure resistance up to 100 MPa (15,000 psi) Low-volume, low-dispersion cartridges minimize the impact on separation �Zero-dead-volume direct connection to column Hand-tight guard replacement (No tools required)
Low-volume, low-dispersion cartridges minimize the impact on separation
EXP®Guard cartridge holder
EXP®cartridge column
Column : YMC-Triart C18 1.9 μm 100 X 2.0 mmI.D.
Eluent : acetonitrile/water (60/40)Flow rate : 0.4 mL/minTemperature : 25°CDetection : UV at 270 nmInjection : 1 μLSample : 1. Uracil
2. Methyl benzoate 3. Naphthalene 4. Butyl benzoate
1
2 3
4
50
100
150
mV
Main column onlyN4 = 19,700
With guard cartridgeN4 = 19,200
0
0 1.0 2.0 3.0 4.0 5.0 min
EXP® guard cartridge column with low-volume and low-dispersion column minimize the impact onseparation. EXP® guard cartridge column provides less than 3% decrease in theoretical plate count.
Hand-tight EXP® fitting
*EXP® is a registered trademark of Optimize Technologies, Inc.
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Quick, easy and
secure connection.
No tools required.Fixing screw
Fixing ferrule
Sealing ferrule(replacable)
Adjuster screw
The tube makes tight contactwith the screw hole bytightening a �xing screw.
Tubing parts
Universal connector
Enables perfect column connectivity A movable ferrule and a fixing screw enable secure connection to
the column endfitting. There is no cavity at the top of the ferrule.
Super high-pressure resistance Universal connectors are high performance connectors resistant
to high pressure up to 70 MPa when tightened only by hand.
For connection to a guard column The double type is the most suitable for connecting of a main
column and a guard column.
Double type
Single type
Tightening the fixing screw forces a tube to squeeze into the column as far as it will go, and the tube makes tight contact with the screw hole.
Universal connector (single)
Product No. Specification
XRSFS520 1/16” outer diameter, 0.005” inner diameter, 20 cm length
XRSFS220 1/16” outer diameter, 0.020” inner diameter, 20 cm length
Universal connector (double)
Product No. Specification
XRSFD506 1/16” outer diameter, 0.005” inner diameter, 6 cm length
XRSFD510 1/16” outer diameter, 0.005” inner diameter, 10 cm length
XRSFD520 1/16” outer diameter, 0.005” inner diameter, 20 cm length
XRSFD210 1/16” outer diameter, 0.020” inner diameter, 10 cm length
XRSFD220 1/16” outer diameter, 0.020” inner diameter, 20 cm length
Universal connector (Single flexible)
Product No. Specification
XRSFS528F 1/32” outer diameter, 0.005” inner diameter, 28 cm length
XRSFS540F 1/32” outer diameter, 0.005” inner diameter, 40 cm length
outer diamater of metted part is 1/16"
Spare ferrule for universal connectors
Product No. Specification
XRSFV Vespel sealing ferrule, 5-pack
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Handy connector 1
A ferrule integrated into a connector Handy connector 1 resists pressure to ca. 21 MPa. Handy connector 1 is suitable
for tubing systems of relatively low pressure. Connection to a tubing system is
easy because a ferrule is integrated into the connector.
Metal free The whole connector is made from PEEK. Handy connector 1 is suitable for
metal-free tubing systems using PEEK tubes.
Handy connector 1
Product No. Specification
XRP0203 For tubing systems of 1/16” outer diameter, 10-pack
Handy connector 2
High pressure resistance and made from stainless steel Handy connector 2 resists pressure to ca. 42 MPa. Handy connector 2 is suitable
for connection to the injection side of a column, to which high pressure is applied.
It is also useful for connection of a guard column or a precolumn filter.
Metal free The ferrule at the wetted part of the connector is made from PEEK. Handy
connector 2 is suitable for metal-free tubing systems using PEEK tubes.
Ferrule is replaceable Only the ferrule needs to be replaced when it deteriorates, as opposed to the
entire connector.
Handy connector 2
Product No. Specification
XRP0204 For tubing system of 1/16” outer diameter, 2-pack
XRP0205 Spare PEEK ferrule, 10-pack
�Be aware that Handy connector 2 cannot be connected to“ Old Shimadzu type”,“ Old JASCO type”, or“ Old Hitachi type” tubes.
PEEK
Stainless steel Ferrule for replacement
(3) Turn the connector while pushing
the tube to connect the tube and
connector.
(4) Be sure that there is no leak from
the screw hole.
(1) Extend the top of PEEK tube
beyond the ferrule.
(2) Force the tube to make tight contact
with the bottom of the screw hole.
Quick, easy and
secure connection.
No tools required.
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Female nut for 1/8” tubing system (cap nut) (stainless steel)
Ferrule for 1/8” tubing system (stainless steel, double ring)
Outer diameter 1/16” Outer diameter 1/8”
Inner diameter0.25 mm 0.5 mm 0.8 mm 2.0 mm
Female nut for 1/8”tubing system
Ferrule for 1/8” tubing system
Male nut for 1/16”tubing system
Ferrule for 1/16” tubing system
W type P type Old Shimadzu type Old Hitachi typeOld JASCO type
PEEK tubing
Easy to use PEEK tubing is made from a material that is flexible and easy to use.
It is easily cut using a commercially available cutting knife. A PEEK
tube with an inner diameter of 0.127 mm is useable at up to 40 MPa.
PEEK tubes are practical general tubing as alternatives to stainless
steel tubing. They are suitable for metal-free tubing systems. A Handy
connector is suitable for connection of PEEK tubing to a column.
Outer diameter 1/16”
Inner diameter0.127 mm 0.25 mm 0.5 mm 0.8 mm
Stainless steel tubing
Stainless steel male nut and ferrule are used for connection
Tube cutter
For cutting 1/16” stainless tubing (simple type)
Male nut for 1/16” tubing system (stainless steel)
Ferrule for 1/16” tubing system (stainless steel)
Union for 1/16” tubing system
Unions for 1/16” tubing system come in two
types, made from either stainless steel or PEEK.
They are convenient to use it with a Handy
connector. Connector type is “W type”.
Stainless steel PEEK
Cross section
Column coupler
Column couplers are used for column-
to-column connections. Various tube
inner diameters and column connection
types are available.
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PEEK tubing
Product No. Specification
XRPT01 1/16” outer diameter, 0.127 mm inner diameter, 3 m length
XRPT02 1/16” outer diameter, 0.25 mm inner diameter, 3 m length
XRPT05 1/16” outer diameter, 0.5 mm inner diameter, 3 m length
XRPT08 1/16” outer diameter, 0.8 mm inner diameter, 3 m length
Stainless steel tubing
Product No. Specification
XRST02 1/16” outer diameter, 0.25 mm inner diameter, 2 m length
XRST05 1/16” outer diameter, 0.5 mm inner diameter, 2 m length
XRST08 1/16” outer diameter, 0.8 mm inner diameter, 2 m length
XRST20 1/8” outer diameter, 2.0 mm inner diameter, 5 m length
Tube cutter
Product No. Specification
XRP1909 For cutting 1/16” stainless tubing
Male nut for 1/16” tubing system (stainless steel)
Product No. Specification
XRONW 1/16” W type, P type, 5-pack
XRONS 1/16” Old Shimadzu type, 5-pack
XRONJ 1/16” Old JASCO type, 5-pack
XRONH 1/16” Old Hitachi type, 5-pack
Ferrule for 1/16” tubing system (stainless steel)
Product No. Specification
XRONFA 1/16” one ring ferrule, 5-pack
Female nut for 1/8” tubing system (cap nut)(stainless steel)
Product No. Specification
XRON8 1/8” female nut, 5-pack
Ferrule for 1/8” tubing system (stainless steel)
Product No. Specification
XRTWFA Front ferrule, back ferrule, 5-pack each
Union for 1/16” tubing system
Product No. Specification
XRUNW 1/16” tubing system (stainless steel)
XRUN16P 1/16” tubing system (PEEK)
Column coupler
Product No. Specification
XRCP1602 1/16” outer diameter, 0.25 mm inner diameter
XRCP1605 1/16” outer diameter, 0.5 mm inner diameter
XRCP1620 1/16” outer diameter, 0.8 mm inner diameter
(Stainless tube 50 mm×1, male nut×2, ferrule×2)
Union for 1/16” tu
bing system
Column coupler
(page 146)
YMC-Pack
guard cartridges
Notes for successful tubing
The shapes of separation peaks are affected not only by column performance, but by the tubing system as well.Some effort is needed to minimize the dead volume in a tubing system among the injector column and detector, in order to prevent poor peak shape. Using a tube with a small inner diameter (0.127 mm) is one solution. When a tube is connected to a union or a column, it is important to give due consideration to make the top of the tube contact flush with the bottom of the screw hole and to avoid leakage or creation of a cavity.
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Empty columns and other parts
1. YMC original empty columns 2. Ferrule type empty column 3. Flange type empty column
Analytical column
Semi-preparative column
Inner diameter 2.0~10 mm
Inner diameter 20~30 mm
Inner diameter 50~200 mm
Inner diameter 4.0~6.0 mm
YMC original empty columns (for analysis or semi-preparative isolation)
A stainless steel tube with a screw and an endfitting are directly connected (screw type). Since YMC original empty columns use no ferrule, there is
no danger of endfitting dislocation when the column is packed and used under high pressure.
YMC original empty columns
Product No.Column size
length X inner diameter (mm)
XC0502WT 50
2.0XC1502WT 150
XC2502WT 250
XC0346WT 30
4.6
XC0546WT 50
XC1046WT 100
XC1546WT 150
XC2546WT 250
XC3046WT 300
XC0506WT 50
6.0
XC1006WT 100
XC1506WT 150
XC2506WT 250
XC3006WT 300
XC5006WT 500
XC0308WT 30
8.0XC3008WT 300
XC5008WT 500
XC0310WT 30
10.0
XC1510WT 150
XC2510WT 250
XC3010WT 300
XC5010WT 500
Product No.Column size
length X inner diameter (mm)
XC0520WT 50
20.0
XC1520WT 150
XC2520WT 250
XC3020WT 300
XC5020WT 500
XCA020WT 1000
XCL530WT 75
30.0
XC2530WT 250
XC3030WT 300
XC5030WT 500
XCA030WT 1000
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YMC original empty column parts
Product No. Specification
XPEF02 For 2.0 mm inner diameter, Column end assembly, 1-pack
XPEF46 For 4.6 mm inner diameter, Column end assembly, 1-pack
XPEF06 For 6.0 mm inner diameter, Column end assembly, 1-pack
XPEF08 For 8.0 mm inner diameter, Column end assembly, 1-pack
XPEF10 For 10 mm inner diameter, Column end assembly, 1-pack
XPEF20 For 20 mm inner diameter, Column end assembly, 1-pack
XPEF30 For 30 mm inner diameter, Column end assembly, 1-pack
XPSF02S For 2.0 mm inner diameter, Frit, 5-pack
XPSF46S For 4.6 mm inner diameter, Frit, 5-pack
XPSF06S For 6.0 mm inner diameter, Frit, 5-pack
XPSF08S For 8.0 mm inner diameter, Frit, 5-pack
XPSF10S For 10 mm inner diameter, Frit, 5-pack
XPSF20S For 20 mm inner diameter, Frit, 2-pack
XPSF30S For 30 mm inner diameter, Frit, 2-pack
XPSS20S For 20 mm inner diameter, Spacer, 1-pack
XPSS30S For 30 mm inner diameter, Spacer, 1-pack
�Frits with 20 mm or more inner diameter include distributors.�Connector type is “W type”.
Main column part names
FritA disk type part used to keep the packing material in a column.
A frit with a pore size of 2 μm is generally used for 5 μm packing
material.
DistributorA part used to uniformly introduce a sample in mobile phase to a
column. Its structure enables uniform diffusion of the sample over
the frit surface.
Column end assembly
Frit
Frit
Spacer
Distributor
Column end assembly
YMC original empty column parts
Inner diameter 2.0 mm, 4.6 mm, 6 mm, 8 mm, 10 mm
Inner diameter 20 mm, 30 mm
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Ferrule type empty column
Ferrule type empty columns are standard-type empty columns. A stainless steel tube and an endfitting are tightly connected using a ferrule.
Ferrule type empty column
Product No.Column size
length X inner diameter (mm)
XC0104WF 10 4.0
XC0105WF 10 5.0
XC0546WF 50
4.6XC1046WF 100
XC1546WF 150
XC2546WF 250
Flange type empty column ( for preparative isolation )
A stainless steel tube with a flange and a cap are tightly connected using a bolt. The inner diameter is 50 mm or more.
Flange type empty column
50 mm inner diameter
Product No.Column size
length X inner diameter (mm)Max. pressure
(MPa)
XC0551■R 50
50.0 7
XC1051■R 100
XC2551■R 250
XC3051■R 300
XC5051■R 500
XCA051■R 1000
XC0552■R 50
50.0 14
XC1052■R 100
XC2552■R 250
XC3052■R 300
XC5052■R 500
XCA052■R 1000
� Designate a frit pore size depending on particle size. We recommend 2 μm for particle size below 10 μm,
5 μm for particle size above 15 μm.
※ ■Connection type: Please select your desired connection type.
A : 1/16" male nut D : 1/8" female nut E : 1/4" female nut
70 mm inner diameter
Product No.Column size
length X inner diameter (mm)Max. pressure
(MPa)
XC1071■R 100
70.0 7
XC2571■R 250
XC3071■R 300
XC5071■R 500
XCA071■R 1000
XC1072■R 100
70.0 14
XC2572■R 250
XC3072■R 300
XC5072■R 500
XCA072■R 1000
� Designate a frit pore size depending on particle size. We recommend 2 μm for particle size below 10 μm,
5 μm for particle size above 15 μm.
100 mm inner diameter
Product No.Column size
length X inner diameter (mm)Max. pressure
(MPa)
XC10A1■R 100
100.0 7XC30A1■R 300
XC50A1■R 500
XCA0A1■R 1000
XC10A2■R 100
100.0 14XC30A2■R 300
XC50A2■R 500
XCA0A2■R 1000
150 mm inner diameter
Product No.Column size
length X inner diameter (mm)Max. pressure
(MPa)
XC20B1■R 200
150.0 7XC30B1■R 300
XC50B1■R 500
XCA0B1■R 1000
XC20B2■R 200
150.0 14XC30B2■R 300
XC50B2■R 500
XCA0B2■R 1000
200 mm inner diameter
Product No.Column size
length X inner diameter (mm)Max. pressure
(MPa)
XC20C1■R 200
200.0 7XC50C1■R 500
XCA0C1■R 1000
XC20C2■R 200
200.0 14XC50C2■R 500
XCA0C2■R 1000
Ferrule type empty column parts
Product No. Specification
XPEF46F For 4.6 mm inner diameter, Column end assembly, 1-pack
XPEF04F For 4.0 mm inner diameter, Column end assembly, 1-pack
XPEF05F For 5.0 mm inner diameter, Column end assembly, 1-pack
XPSF46F For 4.6 mm inner diameter, Frit, 5-pack
XPSF04F For 4.0 mm inner diameter, Frit, 5-pack
XPSF05F For 5.0 mm inner diameter, Frit, 5-pack
Column end assembly
Frit
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Airtight stopper
Airtight stoppers are used to prevent column contents from drying.
Please use a stopper appropriate for each column connection type.
Ferrule type empty column parts
Product No. Product nameMax. pressure
(MPa)
XPCP052 For inner diameter 50 mm, Cap 7
XPCP053 For inner diameter 50 mm, Cap 14
XPSF0522 For inner diameter 50 mm, 2 μm frit (with distributor) 7
XPSF0532 For inner diameter 50 mm, 2 μm frit (with distributor) 14
XPSF0525 For inner diameter 50 mm, 5 μm frit (with distributor) 7
XPSF0535 For inner diameter 50 mm, 5 μm frit (with distributor) 14
XPRI052 For inner diameter 50 mm, Stainless SS ring 7
XPRI053 For inner diameter 50 mm, Stainless SS ring 14
XPBL052 For inner diameter 50 mm, bolt set (8 pairs) 7
XPBL053 For inner diameter 50 mm, bolt set (12 pairs) 14
XPSU162 For inner diameter 50 mm, 1/16” male nut for connection adaptor 7
XPSU163 For inner diameter 50 mm, 1/16” male nut for connection adaptor 14
XPSU82 For inner diameter 50 mm, 1/8” female nut for connection adaptor 7
XPSU83 For inner diameter 50 mm, 1/8” female nut for connection adaptor 14
*For column parts with an inner diameter of 70 mm or more, please contact us.
Specification of flange type empty column
Connection adaptor
Cap
Frit
Distributor
Stainless SS ring
Bolt set
YMC original type W type 1/8" airtight stopper
1/16" airtight stopper
Product No. Specification
XPMSY YMC original type (PEEK), 5-pack
XPMSW Waters type (stainless), 5-pack
1/8" airtight stopper
Product No. Specification
XPMS8 Female nut, 2-pack
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Pressure resistant empty glass columns
Suitable for preparative chromatographyPressure resistant empty glass columns are useful for early
purification of natural products, after-treatment of open column
chromatography and pre-treatment of preparative HPLC.
They are also suitable for removal of impure compounds
produced during organic synthesis.
The most suitable for self-packingLow-cost preparative isolation can be achieved by self-packing.
Pressure resistant empty glass columns are highly flexible tools
for preparative isolation because many types of packing materials
may be packed into the column.
Pressure resistanceYMC empty glass columns, made from Pyrex glass, have
excellent pressure resistance. They are suitable for spherical and
irregular packing materials with a particle size of 50 μm or more.
Applicable to medium-pressure and high-performance liquid chromatography systemsPressure resistant empty glass columns can be connected to
existing chromatographs, making the preparative isolation easy.
Metal free columnNo metal is used for wetted parts. There is no danger of corrosion
caused by acids or of metal contamination in isolation fractions.
Pressure resistant empty glass columns are suitable for a metal-
free HPLC system.
VisibilityIn contrast to stainless steel columns, the separation behavior of
samples analyzed by chromatography can be observed visually.
It is useful for preparative isolation or removal of colored material.
Pressure resistant empty glass columns (glass tube, column endfitting, tubing fitting kit, airtight stopper)
Inner diameter
Product No.
Length
100 mm 300 mm 500 mm
8 mm XC1008FG XC3008FG XC5008FG
20 mm XC1020FG XC3020FG XC5020FG
30 mm XC1030FG XC3030FG XC5030FG
Sam
ple Pretreatm
ent, Accessories
157
Parts
① Glass tube
Inner diameter
Product No.
Length
100 mm 300 mm 500 mm
8 mm XT1008FG XT3008FG XT5008FG
20 mm XT1020FG XT3020FG XT5020FG
30 mm XT1030FG XT3030FG XT5030FG
② Column endfitting (includes frit and packing)
Product No. Specification
XPEA8 For inner diameter 8 mm, 1-pack
XPEA20 For inner diameter 20 mm, 1-pack
XPEA30 For inner diameter 30 mm, 1-pack
One column requires 2 column endfittings.
③ Glass frit (with packing)
Product No. Specification
XPFP83 For inner diameter 8 mm, 3 μm pore size
XPFP2010 For inner diameter 20 mm, 10 μm pore size
XPFP3010 For inner diameter 30 mm, 10 μm pore size
④ Tubing fitting kit (ferrule, o-ring, male nut)
Product No. Specification
XRFK001 For inner diameter 8, 20, 30 mm (common use)
Fitting kit, a set of 2 kits, IN-side and OUT-side, for 1/16” O.D. Teflon tube or stainless tube.
⑤ Special-purpose Teflon tube
Product No. Specification
XRTT102 1/16” outer diameter, 0.25 mm inner diameter, 10 m
XRTT105 1/16” outer diameter, 0.5 mm inner diameter, 10 m
For tubing fitting kits
Column connection joint (column union and flat seal (2-pack))
① Glass tube
Product No. Specification
XRCJ8 For inner diameter 8 mm
XRCJ20 For inner diameter 20 mm
XRCJ30 For inner diameter 30 mm
Used for connection of columns with same diameter in packing.For large columns not described above, please contact us.
1
3
2
4
5
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Lineup of preparative device ---------- 160, 161
Guide for selection devices and columns -- 162
BioStream----------------------------------------- 163
YMC Pilot columns ----------------------------- 164
ECO PLUS---------------------------------------- 165
LC-Forte/R ---------------------------------- 166, 167
K-Prep LAB--------------------------------------- 168
K-Prep FC ---------------------------------------- 168
K-Prep EX----------------------------------------- 169
K series-------------------------------------------- 169
DAD/DAU series ------------------------- 170~172
SB columns --------------------------------------- 173
Software -------------------------------------------- 174
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12
Lineup of preparative device
NameBiochromatography Devices
Multiple PreparativeHPLC Device
BioStream LC-Forte/R
Model BSTP-800BSTP-03KBSTS-03K
BSTS-10K BSTS-30K LC-Forte/R
Appearance
Flow rate range (mL/min)
1 - 800 1 - 3000 1 - 10000 1 - 30000 0.1 - 50.0
Device pressure limit (MPa)
0.5 (Max. 0.6) 30
Dimension (W X D X H mm)
800 X 900 X 1360 900 X 1100 X 1800 1200 X 1200 X 1800 2000 X 1500 X 1800 500 X 500 X 400
Control software BioStream SoftwareLC-Forte/R Software
Forte SeparaⅡ (Optional)
GMP/CSV compliance
Yes No
Explosion proof type
No No
FeatureSanitary design
3 wavelengths measurable at a time
Recycle function3 wavelengths
measurable at a time
See page 163 166, 167
NameBiochromatography Columns
Dynamic Axial Compression Columns
YMC Pilot Columns
ModelPI100/500PI100/850
PI140/500PI140/850
PI200/500PI200/850
PI300/500PI300/850
DAD-50-850SDAU-50-700S
DAD-100-850DAU-100-700S
Appearance
Inner diameter (φ , mm)
100 140 200 300 50, 100
Column length (mm)
500, 850 500, 850 850, 700
Pressure limit (MPa)
1.0 0.7 0.5 0.3 10
See page 164, 165 170 - 172
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ystems
161
Preparative HPLC Devices Explosion Proof Preparative HPLC Devices HPLC Pumps
K-Prep LAB K-Prep FC K-Prep EX K Series
K-Prep LAB100SK-Prep LAB100G
K-Prep LAB300SK-Prep LAB300G
K-Prep FC750SK-Prep FC750G
K-Prep FC1500SK-Prep FC1500G
K-Prep EX03KSK-Prep EX03KG
K-Prep EX06KSK-Prep EX06KG
K-Prep EX12KSK-Prep EX12KG
K-Prep EX25KSK-Prep EX25KG
K-100 K-300 K-500 K-1000
1 - 100 1 - 300 1 - 750 1 - 1500 30 - 3000 60 - 6000 125 - 12500 250 - 25000 1 - 100 1 - 300 1 - 500 1 - 1000
15 10 10 10 10 15 10 10 10
800 X 600 X 650 700 X 750 X 1250 1100 X 1100 X 1300 Please contact us. 225 X 485 X 205 380 X 550 X 220
K-Prep Software LCD panel, Microcomputer control
Yes Yes Yes Yes Yes No
No No No Yes Yes No
Equipped with injectorsfor preparative separation
Equipped with HPLC pumpsto load samples
Custom made devices are available upon request. Standalone operation
168 168 169 169
Dynamic Axial Compression Columns
Self-PackedAxially CompressiblePreparative Columns
SB Columns
DAD-150-850DAU-150-700
DAD-200-850DAU-200-700
DAD-300-850DAU-300-700
DAD-450-850DAU-450-700
DAD-600-850DAU-600-700
Please seepage 173.
150 - 600 (800 and 1000 are available upon request.) 50, 70, 100, 150, 200
850, 700 250, 500, 1000
10 7 10 - 2
170 -172 173
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12
Guide for selection devices and columns
Target component
Low molecular weightorganic compounds
Pharmaceutical products
Agricultural chemicals
Food additives
Natural products
Optical isomers
Biomolecules
Proteins
Peptides
Nucleic acids
Synthetic high-polymer
Macromolecule polymer
Separationmode
Reversed-phase
Normal-phase
Reversed-phase
Normal-phase
Reversed-phase
Normal-phase
Size exclusion
Ion exchange
Size exclusion
Size exclusion
Hydrophobic interaction
Affinity
Recommended columnRecommendedchromatography device
LC-Forte/R YMC-DispoPackAT P.126
YMC-GPC P.124, 125
Recommended column size :φ10 - φ30 mm
P.166,167
P.166,167
P.168
P.168
P.169
P.163
P.168
P.168
P.169
P.166,167
P.166,167
ECO PLUS P.165
YMC-Actus Series P.116~120
Preparative Columns P.121~
Recommended column size :φ10 - φ30 mmECO PLUS P.165
YMC-Actus Series P.116~120
Preparative Columns P.121~
Recommended column size :φ50 - φ150 mmDAD/DAU Series P.170~172
Preparative Columns P.121~
Recommended column size : more thanφ100 mmDAD/DAU Series P.170~172
Recommended column size :φ50 - φ150 mm DAD/DAU Series P.170~172
Preparative Columns P.121~
Recommended column size : more thanφ100 mm DAD/DAU Series P.170~172
YMC Pilot Columns P.164
Recommended column size :φ20 - φ50 mmDAD/DAU Series P.170~172
ECO PLUS P.165
YMC-Actus Series P.116~120
Preparative Columns P.121~
Recommended column size :φ20 - φ50 mmDAD/DAU Series P.170~172
ECO PLUS P.165 YMC-Actus Series P.116~120
Preparative Columns P.121~
LC-Forte/R
K-Prep LAB
K-Prep FC
K-Prep EX
LC-Forte/R
LC-Forte/R
K-Prep LAB
K-Prep FC
K-Prep EX
BioStream
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Specifications
Model BSTP-800 BSTP-03K BSTS-03K BSTS-10K BSTS-30K
Max. flow rate (mL/min) 800 3000 3000 10000 30000
Device pressure limit (MPa) 0.5 (Max. 0.6)
Ambient temperature (°C) 5 - 30
Wetted material PFA, PTFE, Quartz, Glass, EPDM SUS316L, PTFE, Quartz, Glass, EPDM
Sensor pH sensor, Conductivity sensor, Pressure sensor, Flowmeter sensor, UV sensor (3 variable-wavelengths measurable)
Other function Air trap, Air sensor, Column bypass and Column switching
Control software BioStream Software
Dimension (W X D X H mm) 800 X 900 X 1360 900 X 1100 X 1800 900 X 1100 X 1800 1200 X 1200 X 1800 2000 X 1500 X 1800
Weight (kg) 200 250 300 400 600
UtilitySingle –phase 100 V (15 A) Three-phase 200 V (20 A) Three-phase 200 V (30 A) Three-phase 200 V (40 A)
Instrument air, Dry air
BioStream is a biochromatography device and has been developed with YMC’s experience and technology. This system is superior
in operability, performance and quality and it achieves hygiene, speed and high purification which are required by the separation and
purification of biopharmaceuticals.
Biochromatography Devices
BioStream
FeaturesSuitable for downstream processing for biopharmaceutical manufacturing
Compliance with cGMP
Sanitary design superior in cleaning
Excellent operability provided by the largest 21.5-inch touch panel screen in this industry
Low flow pumping provided by the quintuplex diaphragm pump *
Compliance with IQ/OQ validation and CSV
* The pump for BSTP-800 is a triple diaphragm pump.
Advanced flow control
Flow diagram
BioStream is equipped with quintuplex diaphragm pumps and can pump at a low flow rate. The pumps are composed of oil-free materials. It is safe
to use BioStream at production lines. A high-accuracy servo motor and a flowmeter feedback system are combined to achieve extremely high flow-
rate precision.
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YMC Pilot columns are biochromatography columns designed for use in pilot and production scale. All wetted parts are made of
nonmetals. Column design and care in construction avoids causing extra dead volumes and serves to allow the column to be cleaned
easily. YMC Pilot columns are available in AB type, which is suitable for applications with aqueous buffer and in SR type, which are
designed for applications requiring solvent resistant. YMC Pilot columns are widely used from standard normal-phase/reversed-phase
chromatography to use with resins designed for biochromatography.
Biochromatography Columns
YMC Pilot columns
Features�Biocompatible and ideal for use in purification of
biopharmaceuticals such as proteins and peptides, etc.
�Unique frit design enables reduced losses in diffusion and uniform
performance
�Easy scale-up, having the same structure and operability across
different column sizes
Packing bed height easily adjustable by hand wheeled adjusters
Compliance with IQ/OQ validation and FDA regulations
Various options available
Specifications
Model PI100/500 PI100/850 PI140/500 PI140/850 PI200/500 PI200/850
Inner diameter (mm) 100 100 140 140 200 200
Packing bed height (mm) 50 - 430 400 - 780 55 - 420 405 - 770 70 - 435 420 - 785
Volume (L)min 0.39 3.14 0.85 6.23 2.20 13.2
max 3.38 6.13 6.47 11.9 13.7 24.7
Cross-section (cm2) 78.5 78.5 154 154 314 314
Pressure limit (MPa) 1.0 1.0 0.7 0.7 0.5 0.5
Other sizes (more than 300 mmI.D.) are available upon request.
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ECO PLUS glass columns are multi-purpose columns for almost all types of liquid chromatography applications (pressure limit 2.5 to 8.0
MPa). They are available in two forms; AB (Aqueous Buffer) versions for aqueous buffer and cold room applications and SR (Solvent
Resistant) versions for separations involving organic solvents. With a choice of adjustable length plungers at each ends and a totally
inert triple chevron sealing system the SR versions support the complete spectrum of applications from classical normal phase and
reversed phase chromatography through to biochromatography. The “Quick-Lock” connection system makes it possible to open and
seal the column simply and quickly.
Glass Columns
ECO PLUS
Features�Biocompatible
�Universal application
�Solvent resistant version
�Low temperature version available with polyethylene plunger and EPDM sealing ring
�Height adjustable plunger at both ends
�Easy to use
�Compatible with any LC system
Specifications
Model TAC05 TAC10 TAC15 TAC25 TAC35 TAC50
Inner diameter (mm) 5 10 15 25 35 50
Pressure limit (MPa)AB 8.0 8.0 7.0 5.0 4.0 3.0
SR 8.0 5.0 5.0 5.0 4.0 2.5
Column length (mm) 125, 250, 500
Usable temperature range(°C)
AB 4 - 40
SR 16 - 40
Connection1/4" - 28G fittings
(1/16" tubing)1/4" - 28G fittings
(1/8" tubing)
OptionalAB type / SR type
short plunger, short/long plunger, long plunger
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LC-Forte/R, a next-generation device, is designed for both high-/low-pressure chromatography. Key features include recycling mode
and automatic programming. LC-Forte/R is equipped with 3-wavelength UV detector, and ideal for purification of chiral compounds,
functional molecules, and natural products.
Multiple Preparative HPLC Device
LC-Forte/R
Features�Preparative device designed for both High-/Low- pressure chromatography
Ideal for purification in the crude stage through to the final stage
�3 variable-wavelengths UV detector available as a standard feature
�User friendly operation provided by touch input on graphic screen
�Compact design but equipped with multiple functions such as recycling
function, automatic programming function, etc.
�Easy maintenance
Specification
Model LC-Forte/R
Flow rate range (mL/min) 0.1 - 50.0
Device pressure limit (MPa) 30
Dimension (W X D X H mm) 500 X 500 X 400
Control software LC-Forte/R Software
Optional
Available detectors (UV+RI, RI, etc.)
Fraction collector
Software for PC
Inside the device Tubing connections
Max. flow rate
50mL/min
Max. flow rate
50mL/min
Devicepressure limit
30MPa
Devicepressure limit
30MPa
3 variable-wavelengths200-400 nm
3 variable-wavelengths200-400 nm
Low-pressuregradient
Low-pressuregradient
RecyclingRecycling
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Application for recycling chromatographic separation
LC-Forte/R is equipped with recycling function. By using recycling chromatography separation method, higher resolution can be achieved on a sample
that is hardly separated, even without changing mobile phase conditions or columns. Furthermore, no solvent is consumed during recycling. It greatly
contributes to reduction of solvent consumption on purification.
0
100
200
300
400
500
mAU
0 20 40 60 min
1st
2nd
3rd4th
5th
G130904B
Column : YMC-GPC T4000 (10 μm, 10 nm) 600 X 20 mmI.D.
Eluent : chloroformFlow rate : 10 mL/minDetection : UV at 254 nmSample : polystyrene oligomers (50 mg/mL)Injection : 1.4 mLSystem : LC-Forte/R
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K-Prep LAB is an easy to use preparative HPLC member of the K-Prep series prep HPLC’s developed to achieve seamless scale-up.
K-Prep LAB is an easy to learn tabletop preparative system designed for usage at laboratory scale.
Preparative HPLC Devices
K-Prep LAB
Features�Automatic preparative purification device usable at laboratory scale provides
strong support for exploratory research.
�All-in-one device equipped with preparative injectors and fraction collectors
�Fully-automatic operation by PC
�Easy maintenance provided by superior internal structure
�Seamless scaling-up to industrial scale
Max. flow rate
100mL/min
Max. flow rate
mL/min100Devicepressure limit
15MPa
Devicepressure limit
15MPa
UV/VIS195-600
nm
UV/VIS195-600
nm
Gradientmodeselectable
Gradientmodeselectable
K-Prep LAB100
Max. flow rate
300mL/min
Max. flow rate
mL/min300Devicepressure limit
10MPa
Devicepressure limit
10MPa
UV/VIS195-600
nm
UV/VIS195-600
nm
Gradientmodeselectable
Gradientmodeselectable
K-Prep LAB300
Specification
Mode LAB100S LAB100G LAB300S LAB300G
Gradient No Yes No Yes
Flow rate range (mL/min) 1 - 100 1 - 300
Device pressure limit (MPa) 15 10
Fraction collector 20-channel drip method or 5-channel switching valve method
Sample injection Preparative autoinjector
Detector UV/VIS (195 - 600 nm)
Control / Display Notebook computer / Programmable logic controller
Dimension (W X D X H mm) 800 X 600 X 650
Control software K-Prep Software
K-Prep FC is a preparative HPLC device that allows seamless scale-up from small scale production up to industrial scaling-up
processes.
Preparative HPLC Devices
K-Prep FC
Features�Smooth transition from laboratory scale to industrial scale
�Usable for production purpose
�All-in-one device equipped with HPLC pumps to load samples and fraction collectors
�Strong support for preparative purifications provided by fully-automatic operation by PC
�Compliance with IQ/OQ validation and CSV
Max. flow rate
750mL/min
Max. flow rate
mL/min750Devicepressure limit
10MPa
Devicepressure limit
10MPa
UV/VIS195-600
nm
UV/VIS195-600
nm
Gradientmodeselectable
Gradientmodeselectable
CSVcomplianceCSV
compliance
K-Prep FC750
Max. flow rate
1500mL/min
Max. flow rate
mL/min1500Devicepressure limit
10MPa
Devicepressure limit
10MPa
UV/VIS195-600
nm
UV/VIS195-600
nm
Gradientmodeselectable
Gradientmodeselectable
CSVcomplianceCSV
compliance
K-Prep FC1500
Specification
Model FC750S FC750G FC1500S FC1500G
Gradient No Yes No Yes
Flow rate range (mL/min) 1 - 750 1 - 1500
Device pressure limit (MPa) 10
Fraction collector 5-channel switching valve method
Sample injection HPLC pump to load samples (1 - 300 mL/min)
Detector UV/VIS (195 - 600 nm)
Control / Display Notebook computer / Programmable logic controller
Control software K-Prep Software
Utility AC100V, 0.5 MPa dry air
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K-Prep EX is an explosion proof HPLC preparative device designed for GMP manufacturing plants.
Explosion Proof Preparative HPLC Devices
K-Prep EX
K series pumps are designed for preparative chromatography employed worldwide for simple and demanding applications. K-Prep
dual reciprocating plungers and the high precision servo motor enable highly-accurate pumping with less pulsation.
HPLC Pumps
K series
Features�A wide range of flow rates and range allow a minimum flow rate 1/1000 of the maximum.
�Usable as a high pressure mixing gradient system by adding on a sub-pump
�Gradient and flow rate programmable provided by microcomputer control
�Max. pressure limit can be set enabling column overpressure protection.
�Plunger wash ports (optional) to prevent salt deposition and premature seal failure
�Explosion proof pumps (optional) available
Features�Explosion proof device
�This explosion proof HPLC unit may be installed in a hazardous area
and controlled from a safe area
�Operable at hazardous area by operation station (optional)
�Custom-made device available upon request
�Compliance with IQ/OQ validation and CSV
�Documentation maintenance feature allows for adjusting recordkeeping
to meet your needs
Explosionproof
Explosionproof
UV/VIS195-600
nm
UV/VIS195-600
nm
Gradientmodeselectable
Gradientmodeselectable
CSVcomplianceCSV
compliance
Specification
Model K-100 K-300 K-500 K-1000
Flow rate range (mL/min) 1 - 100 1 - 300 1 - 500 1 - 1000
Device pressure limit (MPa) 15 10 10 5
Pumping mechanism Dual plunger linear cam driven
Pumping method Constant flow
Wetted material SUS316, Zirconia, Reinforced Teflon, PEEK
Dimension (W X D X H mm) 225 X 485 X 205 380 X 550 X 220
Flow diagram
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The DAD series are an automatic self-packing type of dynamic axial compression column that allows for an easy, cost-effective
refill of packing material that results in a column bed yielding superior durability and reproducibility. The DAD series are available in
columns for laboratory, pilot, and industrial scale.
Dynamic Axial Compression Columns
DAD series: Driving direction of cylinder is downward
Features�Suitable for high purification in various fields such as pharmaceuticals, fine chemicals and functional foods
�Cost-effective self-packing dynamic axial compression columns
�Optional slurry container permits automated packing procedures.
�Superior column performance, durability and reproducibility provided by usage at constant pressure
�Easy unpacking operation with downward cylinder driving direction
�No need to prepare lifting equipment after installation
�Compliance with IQ/OQ validation
Specification
Model DAD-50 DAD-100 DAD-150 DAD-200 DAD-300 DAD-450 DAD-600 DAD-800 DAD-1000
Inner diameter (φ, mm) 50 100 150 200 300 450 600 800 1000
Pressure limit (MPa) 10 7
Column length (mm) 850
Column bed length (mm) 100 - 500
Slurry container volume (L)* 2 10 22 30 80 160 300 600 1200
* Slurry containers are optional.
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The DAU series are an automatic self-packing type of dynamic axial compression column that allows for an easy, cost-effective
refill of packing material that results in a column bed yielding superior durability and reproducibility. DAU columns have been selling
more than 10 years and have a good reputation of maintaining stable performance over years after installation. The DAU series are
available in columns for laboratory, pilot, and industrial scale.
Dynamic Axial Compression Columns
DAU series: Driving direction of cylinder is upward
Features�Suitable for high purification in various fields such as pharmaceuticals, fine chemicals and functional foods
�Cost-effective self-packing dynamic axial compression columns
�Optional slurry container permits automated packing procedures
�Superior column performance, durability and reproducibility provided by usage at constant pressure
�Compact and sophisticated design based on our abundant experience
�Compliance with IQ/OQ validation
Specification
Model DAU-50 DAU-100 DAU-150 DAU-200 DAU-300 DAU-450 DAU-600
Inner diameter (φ, mm) 50 100 150 200 300 450 600
Pressure limit (MPa) 10 7
Column length (mm) 700
Column bed length (mm) 100 - 400
Slurry container volume (L)* 2 10 22 30 60 140 250
* Slurry containers are optional.
Slurry container
The slurry container provides excellent
distribution of packing material and allows for a
“homogeneous” slurry that can be automatically
loaded into the DAU/DAD column by simply
switching a valve. Throughout the operation
from slurry preparation to filling slurry, safety and
hygiene can be achieved.
P
Circulation
Column
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12
Packing procedure
Column performance
The data on the right hand side shows repacking data obtained by
using a 50 mmI.D. DAU column(DAU-50). After 7 times repacking,
plates/m and Tf are still as good as the initial state.
plat
es/m
Tf
Change in chromatogram Change in column performance
Seamless scale-up
The chromatograms on the right hand side are a scale-up example
from an analytical 4.6 mmI.D. column to a 50 mmI.D. DAU column.
This indicates that the very similar separation pattern of analytical
scale is also reproducible at the preparative scale.
analytical
Scale up
preparative
Column : YMC*GEL C8 (S-10 μm, 20 nm) 150 X 50 mmI.D.
Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 25-60%B (0-20min)
Flow rate : 100 mL/minTemperature : 37°CDetection : UV at 220 nm
Column : YMC-Pack C8 (S-5 μm, 20 nm) 150 X 4.6 mmI.D.
Flow rate : 0.8 mL/min
1.Cytchrome c MW 124002.Insulin MW 57003.Lysozyme MW 144004.α-Lactalbumin MW 142005.Myoglobin MW 17000
Dynamic Axial Compression Columns
Ease of use and outstanding column performance in a single system
Fill slurrySlurry solventevacuation
Compression Completion of a column
Slurrysupport
Fill packing materialthrough slurry pump.
Perform chromatography.Compress the columnand push out extra slurrysolvent.
Slurry
Support
Packing/compression Operation
DAU Series : Refer to the drawings.
DAD Series : Compressive direction is reversed.
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173
SB products are statically compressed cost effective preparative columns which enable convenient self-packing. Tightening screw
bolts of the upper flange yields a compressed packing bed. Not only dry packing but also wet (slurry) packing is possible by attaching
a column packing extender (optional).
Self-Packed Axially Compressible Preparative Columns
SB columns
Features�Superior column performance and reproducibility provided by high density packing
Maintains great durability utilizing manual pressurization using screw bolts
Eliminate voids (occurred by long-term usage) by re-tightening screw bolts
Cost effective provided by self-repacking
Packing service (optional) available with YMC’s preparative packing materials
Column packing extender (optional) and column stand (optional) available
Specification
Model SB-50 SB-70 SB-100 SB-150 SB-200
Inner diameter (φ, mm) 50 70 100 150 200
Pressure limit (MPa) 10 7 7 5 5
Column length (mm) 250 / 500 / 1000
Column bed length (mm) 200 - 300 / 450 - 550 / 900 - 1100
OptionalColumn packing extender
Column stand
Please contact us for SB Columns with 300 mmI.D. or larger.
Flow Reactor
13
What’s Flow Reactor --------------------------- 176
KeyChem® Series------------------------------- 177
Micro Flow Reactor System ------------------- 177
Hydrogenation Flow Reactor System------- 177
Flow Photoreactor System--------------------- 177
Heterogeneous Flow Reactor System------ 178
Reactor --------------------------------------------- 179
Pump------------------------------------------------ 179
Accessories・Consumable parts ----------- 180
Flow Reactor
Flow
Reactor
176
13
In a conventional batch system (flask), synthesizing of a target compound (No.1 above) alone has been extremely difficult when a
product is highly reactive. This is because undesired production of a non-target compound (No. 2 above) due to further reaction of the
product takes place even at low temperature.
In a flow micro reaction system, residence time of a highly reactive compound can be kept short just by adjusting the flow rate, and
a rapid reaction with DMF by micromixing minimizes the undesired production of byproducts. Since there is no need to keep the
temperature low, less energy cost is required.
Reaction example
Exothermic reaction
Using precise temperature control
Reaction including unstable intermediate
Keep residence time short
Using precise temperature control
Consecutive reaction
Keep residence time short
Generated fine particles and emulsions
Precise particle size and size distribution control
Because of generated in the micro space
Reactions depend on surface area
Because of a large surface area , contact efficiency increase
High efficiency reaction using Microreactor
What’s Flow Reactor
Micro-flow reaction (Flow Reactor) is a new solution performing chemical reaction by delivering raw
materials into micro channels. Flow Reactor enables various types of reactions which could not have
been performed in a conventional batch mode (flask, vessel).
What is ‘‘Flow Reactor’’
React of products each other
Improving Selectivity
React with DMF before reacting each other
Flow
Reactor
177
KeyChem® Series
KeyChem® is an abbreviation for keyboard chemistry. “Keyboard chemistry®” has been coined by YMC by combining the words
“Keyboard” (as in computer control) and “Chemistry”, to represent the new concept of a chemical reaction methodology that YMC is
creating. Central to this concept is the microreactor system, which allows computer-controlled flow reaction and enables the setting of
all the conditions of a chemical reaction from start to finish, via the keyboard of a computer.
Reaction type Other
Liquid-Liquid Gas-Liquid Solid-Liquid Gas-Solid Photoreaction PC Control Customization
KeyChem®-Basic Yes No No No No No Yes
KeyChem®-L/LP Yes No No No No Yes Yes
KeyChem®-H No No Yes Yes No Yes Yes
KeyChem®-Lumino No No No No Yes Yes Yes
KeyChem®-Integral Yes Yes Yes Yes No Yes Yes
*The above products can be customized to add reaction type.
Wide variety including Liquid-Liquid reaction system for rapid mixing of reaction liquid, and system specializing in hydrogenation or
photoreaction
Product Comparison Table
What is “KeyChem®”
• Micro-flow starter kit to get started quickly
Type-1 : Simple system
Type-2 : Standard system
Type-3 : Two-stage reaction system
Type-4 : System equipped with thermal control unit
• Other types are available upon request.
• Superior mixing, precise thermal control, continuous-flow reactor
• Set conditions of reaction by numerical entry into PC controlled system
KeyChem®-L : Syringe pump type for stable pumping
KeyChem®-LP : Plunger pump type for continuous pumping
• Optional extra : Fraction collector
Micro Flow Reactor System
KeyChem®-BasicPerform flow reaction easily
KeyChem®-L / KeyChem®-LPFlow reactor system by PC control
• Hydrogen supply type using harmless hydrogen storing alloy
• High level contact of catalyst and raw materials by catalyst
column
• Flow rate and backpressure are controllable.
• Uniform light irradiation to sample, introducing micro channels
• Materials of light source and reactor are selectable.
• Achieve precise photoreaction
Hydrogenation Flow Reactor System Flow Photoreactor System
KeyChem®-HNow available safe and highly-efficient hydrogenation
reactor
KeyChem®-LuminoExpand possibility of photoreaction by superior efficiency
of light irradiation
Flow Reactor
Flow
Reactor
178
13
Heterogeneous Flow Reactor System
• Single system plays 4 roles : Reaction of Gas-Liquid,
Liquid-Liquid, Solid-Liquid and Solid-Liquid-Gas
• Porous Teflon® (AF2400) is used for the reactor of Gas-
Liquid reaction
• Flow rate, temperature and pressure are controllable.
• The number of units and combinations are selectable.
• Flow micro mixer for Liquid-Liquid
reaction
• Improvement on reaction yield
with rapid mixing in micro space
feature
KeyChem®-IntegralHeterogenerous Reactions In Single System
Specifications
Mixing Block Unit
• Reactor for Gas-Liquid reaction
made of Teflon® AF2400
• Dispersing gas gradually into
liquid flowing in a SUS tube
through porous of Teflon®
• Mixing gas and reaction liquid
homogeneously
Coil Reactor Unit
• Sending solution by plunger pump
• Highly accurate and stable in flow
rate
• Available in 3 types according to
flow rate range
• Available wetted material : PEEK or
SUS
Pump Unit
• Catalyst column packed with
reaction liquid
• High reaction efficiency with
high level contact of catalyst
and reaction liquid
• No need to remove catalyst
Column Reactor Unit
Pump unit Material SUS resin SUS resin SUS resin
Type Plunger
Range 0.001 to 1.00 mL/min 0.01 to 10.0 mL/min 0.1 to 50.0 mL/min
Pressure resistance 25 MPa 20 MPa 25 MPa 20 MPa 5 MPa 5 MPa
Dimensions (W X D X H) 140 X 186 X 123 mm
Coil Reactor Unit Low Temperature Type High Temperature Type
Method Peltier Heating
Range 5 to 80℃ r.t.+10℃ to 200℃
Dimensions (W X D X H) 250 X 225 X 175 mm
Mixing Block Unit Low Temperature Type High Temperature Type
Method Peltier Heating
Range 5 to 80℃ r.t.+10℃ to 200℃
Dimensions (W X D X H) 250 X 225 X 175 mm
Column Reactor Unit
Method Thermal Vibration
Range r.t.+10℃ to 200℃
Dimensions (W X D X H) 250 X 225 X 347 mm
Flow
Reactor
179
Pump
Reactor (KeyChem® series mixer)
• Deliver constant volumes and microliter volumes
• Change flow rate during operation
• A variety of types available to suit your needs
• Compatible with various syringe sizes and types from
major manufacturers
YSP series Syringe pump
• Mixing on collision in simple
Y-shaped channel
• Ideal for synthesis from drop
Material Model number
SUS316 KC-M-Y-SUS
Glass KC-M-Y-GL
PTFE KC-M-Y-P
Hadar (Y type)
• Powerful agitation, structure suitable
for organic synthesis
• Mixing after 3D motion
Material Model number
SUS316 KC-M-H-SUS
Glass KC-M-H-G
PTFE KC-M-H-P
Deneb (Helix type)
• mixing after repeated bifurcation and
merging
Material Model number
SUS316 KC-M-S-SUS
Glass KC-M-S-G
PTFE KC-M-S-P
Spica (Static type)
High-performance and cost-effective syringe pumps
Usable for micro-flow reaction, dripping reagents, pharmacological experiment, animal testing, etc.
YSP-101 (Standard type)Infuse only and no capability to withdraw
YSP-201(High-performance type)Similar to YSP-101 but with withdrawal
function.
YSP-301(High performance & high pressure type)Includes features of YSP-201 and high
pressure pumping function.
YSP-202(Double syringe type)Similar to YSP-201 but holds 2 syringes.
Model number YSP-101 YSP-201 YSP-301 YSP-202
Syringe type Standard type High-performance type High performance & high pressure type Double syringe type
Infusion Yes Yes Yes Yes
Withdrawal No Yes Yes Yes
Programmable pump No Yes Yes Yes
PC Contorol No Yes Yes Yes
Minimum flow rate*1 0.012 μL/min 0.023 μL/min
Maximum flow rate*2 31.5 mL/min 42.57 mL/min 153.3 mL/min 76.32 mL/min*3
The pumps may not be used at max. or min. flow rate under certain conditions.*1 Obtained using a 1mL disposable syringe. *2 Obtained using a 50mL disposable syringe. *3 Obtained using a 25mL gastight syringe.
Three kinds of micro-mixers with different mixing configurations are abiale.
Flow Reactor
Flow
Reactor
180
13
Accessories・Consumable parts
Connector 1/16 in. SUS Connector 1/16 in. PEEK, M6HN Connector 1/8 in. PEEK, M6HN
Model number : YMC-P-0001
Material : SUS316
Quantity : 10
Connecting 1/16 in. ext. diam. tube
Model number : YMC-P-0002
Material : PEEK
Quantity : 1
Used in conjunction with ferrule (YMC-P-0012). Connecting mixer housing with 1/16 in. ext. diam. tube
Model number : YMC-P-0067
Material : PEEK
Quantity : 1
Used in conjunction with ferrule (YMC-P-0068). Connecting 1/8 in. ext. diam. tube.
Connector for KeyChem®series Connector for KeyChem®series (SUS) Connector 1/16 in. (10 pcs., plastics)
Model number : YMC-P-0005
Material : PEEK
Quantity : 1
Connecting thermostat Thermo Stage of KeyChem® series. Used in conjunction with ferrule (YMC-P-0012). Connecting with 1/16 in. ext. diam. tube.
Model number : YMC-P-LU06
Material : SUS304
Quantity : 1
Connecting reactor housing of Lumino.Used in conjunction with ferrule (YMC-P-0012). Connecting with 1/16 in. ext. diam. tube.
Model number : YMC-P-0006
Material : ETFE
Quantity : 10
Used in conjunction with ferrule (YMC-P-0012). Connecting 1/16 in. ext. diam. tube to syringe adaptor (female type) (YMC-P-0007), lure adaptor (male type) (YMC-P-0008)
Housing connector (for SUS) Syringe adaptor (female type) Lure adaptor (male type)
Model number : YMC-P-0057
Material : SUS
Quantity : 1
Connecting 1/16 in. ext. diam. SUS tube to housing.
Model number : YMC-P-0007
Material : ETFE
Quantity : 1
Adaptor connecting syringe (lure lock type) to tube. Used in connection with 1/16 in. plastic connector (YMC-P-0006).
Model number : YMC-P-0008
Material : ETFE
Quantity : 1
Adaptor connecting syringe needle. Used in reaction including water-reactive substance and in connection with 1/16 in. plastic connector (YMC-P-0006).
EASYFITT10φ Connector 1/8 in. (ETFE and FSF) Ferrule 1/16 in. (plastic)
Model number : YMC-P-0009
Material : PEEK
Quantity : 10
Combined fitting and ferrule connector. Connected by 1/16 in. ext. diam. tube.
Model number : YMC-P-0070
Material : ETFE
Quantity : 10
Connecting 1/8 in. tube and plunger pump.
Model number : YMC-P-0010
Material : PTFE
Quantity : 10
Used in conjunction with 1/16 in. connector (SUS, YMC-P-0001). Mainly used in PTFE tube.
Ferrule 1/16 in. (SUS) Ferrule 1/16 in. (PTFE and GF) Ferrule 1/8 in. (PTFE and GF)
Model number : YMC-P-0011
Material : SUS316
Quantity : 10
Used in conjunction with 1/16 in. connector (SUS, YMC-P-0001). Mainly used in SUS tube.
Model number : YMC-P-0012
Material : PTFE, SUS316
Quantity : 10
Ferrule connecting connector 1/16 in. PTFE,M6HN (YMC-P-0002) and 1/16 in. ext. diam. tube.
Model number : YMC-P-0068
Material : PTFE, SUS316
Quantity : 10
Ferrule connecting connector 1/8 in. PTFE,M6HN (YMC-P-0067) and 1/8 in. ext. diam. tube.
Ferrule 1/16 in. (SFL) Ferrule 1/8 in. (ETFE and FSF) Mixer housing for KeyChem®
Model number : YMC-P-0060
Material : PEEK, SUS316
Quantity : 10
Ferrule connecting connector 1/16 in. and 1/16 in. ext. diam. tube. Applicable to SUS tube as well.
Model number : YMC-P-0069
Material : ETFE
Quantity : 10
Used in conjunction with connector 1/8 in. (ETFE and FSF, YMC-P-0070).
YMC highly pressure-resistive syringe Disposable syringe
Model number : YMC-P-1014
Material : 25 mL
Quantity : 1
YMC original highly pressure-resistive syringe. Easy to install on MR2 pump.
Capacity 2 mL 5 mL10 mL20 mL30 mL50 mL
Quantity1001001001005030
Model numberYMC-P-1001YMC-P-1002YMC-P-1003YMC-P-1004YMC-P-1021YMC-P-1022
PTFE Tube SUS Tube
In. diam. Model number
Ext. diam. : 1/16 in.Length : 10 m
0.25 mm0.5 mm0.75 mm1.0 mm
YMC-P-0024YMC-P-0025YMC-P-0026YMC-P-0027
Ext. diam. : 1/8 in.Length : 10 m
1.6 mm2.4 mm
YMC-P-0075YMC-P-0076
In. diam. Model numberExt. diam. : 1/16 in.Length : 10 m
0.5 mm1.0 mm
YMC-P-0028YMC-P-0029
Model number :YMC-P-0030-01Material : aluminum
Model number :YMC-P-0030-02Material : aluminum
Model number :YMC-P-0031Material : acrylic resin
Preparative Systems
Preparative S
ystems
174
12
Software allowing seamless scale-up from laboratory scale to process scale
User friendly, flexible and intuitive programming and operation
Compliance with FDA 21 CFR Part 11, cGMP and CSV
Software (for Chromatography Devices)
K-Prep, BioStream
Liquid crystal touch panel allows comfortable visual touch.
Standard features include software for displaying chromatograms on the touchscreen.
Various functions are available such as recycling, sample injection (stacking), and automated cleaning.
�When operated in conjunction with optional external PC control software quantitative (area%) and GPC measurement may be
employed.
Software (for Chromatography Devices)
LC-Forte/R
CSVCSVcGMPcGMPFDA21 CFR Part 11
FDA21 CFR Part 11
Software for K-Prep and BioStream are the
latest chromatography device control software.
The software has been developed to be intuitive
based on the operator’s reaction to visual
operation. The software enables complicated
preparative purifications to be performed easily
and automatically.
The dedicated software for LC-Forte/R has been developed to put the full power of the instrument within your grasp.
Information
16
Column handling-------------------------------- 194
Mobile phases for ODS columns ----------- 195
HPLC column performance ------------------ 196
Inspection reports ------------------------------ 197
Basic data ---------------------------------------- 198
Full listing of all
chemistries and dimensions ----------------- 199
Information
Information
194
16
Column handling1 Introduction 2 Shipping solventYMC CO., LTD. produces HPLC columns under strict quality control and delivers to customers only those products that pass the column tests prior to shipment. In order to ensure peak functionality and long life of the column, please follow these instructions.
The solvent enclosed at shipment is described in the COLUMN INSPECTION REPORT or instruction manual included with each column. Please pay attention to the miscibility of the solvent with the mobile phase used in your analysis. If you intend to store the column for a long time, replace the mobile phase in the column with the shipping solvent or solvent specified in the Column Inspection Report.
3 Mobile phase
4 Mobile phase replacement and column cleaning (general methods)
5 Guard columns
6 Other environments
Use n-hexane, chloroform or other weak solvents and add isopropanol and ethyl acetate or similar as appropriate to speed up elution of high-polarity components.・ Amino columns (PolyamineⅡand NH2) can be used with
both aqueous and non-aqueous solvents. However, repeated alternate use of solvents with extremely different polarity may diminish column performance.・ Solvent should flow in the direction of the arrow as indicated
on the column label.・ The usable pH ranges of columns vary by product. Please
refer to the instruction manual available on our website.
・ Reversed-phase columns can be used with both aqueous and non-aqueous solvents. However, repeated alternate use of solvents with extremely different polarity may diminish column performance. Usable general organic solvents include acetonitrile, methanol and THF.・ CN columns can be used in both normal-phase and reversed-
phase modes. However, a column should be dedicated for each separation mode. When using the column in a normal-phase mode, replace the solvent in the column with 2-propanol. (Be sure to set the flow rate so that the pressure is at 15 MPa or less.)・ SIL columns are usually used with non-aqueous solvents.
the same composition as that of the mobile phase, but with a higher organic solvent concentration is usually used as the cleaning solution, but consideration should be given with respect to the sample characteristics to select a solvent which easily dissolves the sample.・ When macromolecules, including proteins and sugars,
adsorb to the column, it is usually difficult to wash out the macromolecules with organic solvents. When a sample containing macromolecules is analyzed, it is preferable to conduct pretreatment and/or use a guard column.
(B) Normal-phase columns・ Wash the column with a solution having the same solvents
as that of the mobile phase, but with a high polar component concentration that is higher than that of the mobile phase. If polar compounds absorb to the column, flush with isopropanol or similar solvent.・ When a mobile phase containing acid or alkaline is used,
replace it with a non-acid, non-alkaline solvent before storage. (eg., n-hexane/2-propanol/acetic acid (90/10/0.1) → n-hexane/2-propanol (90/10))
(A) Reversed-phase columns・ When a mobile phase containing neither buffer nor salts
is used, wash the column with a solution having the same solvents as that of the mobile phase, but with a higher organic solvent concentration.・ When a mobile phase containing buffer or salts is used,
replace it with a mixture of water and organic solvent (at the same ratio as the mobile phase) containing neither buffer nor salts.
When a mobile phase containing buffer or salts is used at about 50 mM, it can be replaced directly with about 60% acetonitrile aqueous solution.・ After using a column near the usable pH limit, washing the
column with water alone may cause column deterioration. Use the mixture of water and organic solvent described above or about 60% acetonitrile aqueous solution to replace the mobile phase.・ When column pressure increases, flow the solution through
the column in the reverse direction (the opposite direction of the arrow shown on the column label). A solution having
・ For an analytical column for which both a guard cartridge and a packed column-type guard column are available, select the guard cartridge or the packed column-type guard column according to the expected replacement frequency of the guard column. We recommend a guard cartridge if samples containing a lot of impurities require the guard column to be replaced frequently.
・ Use a guard column with the same packing material and the same or smaller inner diameter as that of the main column.・ A YMC guard cartridge is composed of a cartridge holder
and a guard cartridge column. The cartridge holder can be used repeatedly. A guard cartridge with an inner diameter of 4.0 mm can be used with analytical columns with inner diameters of 4.0 to 6.0 mm.
pressure to increase. Filter samples through a YMC Duo- Filter (0.2 μm) before injection. Use a precolumn filter for a sample that may clog the frit of the column.
・ Column pressure limit and temperature range of columns vary by product. Please refer to the instruction manual available on our website. ・ Repeated injection of samples may cause the column
Information
195
The composition of mobile phase greatly affects the separation in HPLC. To optimize a separation, it is necessary to consider the
interaction of the solutes, stationary (or solid) phase, as well as the mobile phase.
For ODS columns, commonly used in HPLC, various mobile phases are available. Attention needs to be paid to many points when
deciding on the mobile phase composition. The variables include the following factors: miscibility of solvents, effects on detection
methods (eg., UV or MS), effects on the column (eg., column deterioration due to pressure or pH), separation reproducibility and
the stability of solutes. Useful solvents for ODS columns and some helpful tips for establishing optimum separation conditions are
described below.
Mobile phases for ODS columns
General solvents
Water, acetonitrile (ACN), methanol (MeOH) and tetrahydrofuran (THF) are important solvents for use with ODS columns.It is important to use high purity water purified by ion-exchange, distillation, reverse osmosis, or the like. The presence of organic substances or ionic impurities may cause problems, including ghost peaks during short wavelength UV detection.ACN is frequently used as an HPLC solvent, due to its low UV absorption and low viscosity. MeOH has a higher viscosity and often shows different separation selectivity from that of ACN. THF is sometimes used to influence selectivity along with ACN and MeOH. The cyclic ether structure of THF may result in different separation characteristics from those of ACN and MeOH. Be aware that THF has significant UV absorption and take notice of its viscosity and peroxides. Usually, THF containing no antioxidants is used for HPLC.Find the appropriate separating conditions by using these three types of organic solvents individually or in combination.
Buffers and reagents
Acetic acid, phosphoric acid and trifluoroacetic acid (TFA) are generally used as acidic modifiers.The buffers normally used include phosphate buffer (sodium, potassium, ammonium) and acetate buffer. Monobasic phosphates yield about pH 4.6 and are used as convenient pH adjusters by themselves.In order to separate an ionic compound, such as amine and carboxylic acid, with good repeatability, the pH of mobile phase must be controlled. It is desirable to adjust the pH of the mobile phase so that it is one or more units away from the pKa of the solute. Near the pKa, peak broadening or splitting may be observed.These buffers are usually used at a concentration of about 10 mM. However, depending on dissociation of solutes and interaction with the stationary phase, these buffers may be used at 50 to 100 mM. When acids or alkalines that are likely to deteriorate ODS are used, caution must be taken regarding their concentrations and pH. TFA and phosphoric acid are usually used at concentrations of 0.1% or less.Approximately 60% acetonitrile aqueous solution is a convenient solvent for storage after the use of acids or buffers (salts).Tetrabutylammonium salts and sodium perchloric acid may be used as ion pair reagents for retention of highly polar compounds on ODS or for improvement of separation and peak shape. When these additives are used, it is necessary to use a reagent with the shortest alkyl chains available. If sodium dodecylsulfate, (SDS; which contains long alkylchains) is used, the SDS is likely to be retained on ODS and sometimes cause problems with repeatability.
Others
Ethanol, 2-propanol, ethyl acetate, or chloroform may be added to the mobile phase in order to improve retention or separation of solutes. In some cases, hexane is used as a mobile phase. When a hydrophobic solvent is used as a mobile phase, caution is required regarding the miscibility of the solvent with the mobile phase in the column.
Information
Information
196
16
Important factors used in the evaluation of column performance include column efficiency, capacity, separation characteristics of
solutes, peak shape, and column pressure. Elution curve management in partition chromatography, including ODS, at YMC CO., LTD.
is described below.
Column efficiency, an important characteristic for evaluation of column performance, is generally measured in terms of theoretical
plate number. Usually, the theoretical plate number is calculated using bandwidth at half-height. Narrower peak widths result in higher
theoretical plate numbers. Longer columns and smaller packing material particle size tend to result in higher theoretical plate numbers.
Due to a variety of factors, one column does not always show the same theoretical plate number. This may be caused by differences
between linear velocity and solute diffusion in the column or because of interaction between solutes and the mobile phase or the
stationary phase.
For these and other reasons, column efficiency is solute specific and the measurment of efficiency must be conducted under nearly
identical HPLC conditions for results to be directly comparable.
Retention and separation characteristics for solutes on the column are evaluated by the capacity factor and separation factor values.
These values are indexes of the packing material characteristics and, in contrast to the retention time, do not depend on column inner
diameter and length.
Elution peak shape is also an important factor for evaluation of column performance. The asymmetry factor is a relatively simple
evaluation measure, generally calculated at 10% of peak height.
HPLC column performance
t0
tR1tR2
h
h/2
h/10h/20
W0.5h1 W0.5h2
W0.05h
a b
f
Void volume, Column dead-time
Retention time
Peak height
Bandwidth at half-height
Theoretical plate number N=5.54×(tR/W0.5h)2
Capacity factor R-tO)/tO
Separation factor 2 1
Resolution Rs=1.18×(tR2-tR1)/(W0.5h1+W0.5h2)
Asymmetry factor As=b/a
Tailing factor Tf=W0.05h/2f
tO
tR
h
W0.5h
N
α
RS
AS
Tf
Information
197
YMC CO., LTD. conducts quality control of packing materials to ensure lot-to-lot and column-to-column reproducibility. YMC CO., LTD.
tests in principle the performance of all columns and delivers only those products that meet strict specifications.
A test report of the kind that is shipped with each column is shown below.
The analysis conducted at the delivery inspection is not only a method for column performance evaluation but also a useful test
method for determination of column life. YMC CO., LTD. describes all analytical conditions in the test reports of main products,
including sample concentration, for convenience in evaluation of column deterioration by the end user.
Inspection reports
Information
Information
198
16
Unit Conversion
SI Prefixes
Column Area Ratio
Pressure
MPa bar psi kgf/cm2 atm
1 10 145.04 10.20 9.87
0.1 1 14.504 1.020 0.987
6.90 X10-3 0.069 1 0.070 0.068
0.0981 0.981 14.223 1 0.968
0.101 1.013 14.696 1.033 1
da(deca) h(hecto) k(kilo) M(mega) G(giga) T(tera) P(peta) E(exa) Z(zetta) Y(yotta)
101 102 103 106 109 1012 1015 1018 1021 1024
d(deci) c(centi) m(milli) μ(micro) n(nano) p(pico) f(femto) a(atto) z(zepto) y(yocto)
10-1 10-2 10-3 10-6 10-9 10-12 10-15 10-18 10-21 10-24
* 1 Å (ångström) = 0.1 nm = 10-10 m
Inner Diameter 1.0 2.0 3.0 4.6 10.0 20.0 30.0 50.0
Ratio 0.0473 0.189 0.425 1 4.73 18.90 42.53 118.15
Weight
kg oz lb
1 35.274 2.204
0.0283 1 0.0625
0.454 16 1
Temperature
K ℉ ℃
0 -459.67 -273.15
255.37 0 -17.8
273.15 32 0
298.15 77 25
310.93 100 37.8
373.15 212 100
formula: ℃= (℉−32) X5/9 ℉=℃ X9/5 +32
Length
m in ft yd mile
1 39.37 3.28 1.094 6.21 X10-4
0.025 1 0.083 0.028 0.15 X10-4
0.305 12 1 0.33 1.89 X10-4
0.91 36 3 1 5.68 X10-4
1609.3 63360 5280 1760 1
Volume
L gal (UK) gal (US)
1 0.22 0.26
4.55 1 1.201
3.79 0.83 1
Ratio Scale
ppb ppm %
1 10-3 10-7
103 1 10-4
107 104 1
Basic data
Information
199
Ordering InformationThe previous product listing represents commonly used standard column dimension. In order to identify any specific product version
and order number, please see the example and the table below.
Full listing of all chemistries and dimensions
Gel Code Hardware Code
Chemistry codePore size
(Å)Particle shape
Particle size(μm)
Length(mm)
Inner diameter(mm)
Column Type
Triart C18Triart C18 ExRSMeteoric Core C18Meteoric Core C18 BIOPro C18Hydrosphere C18Pro C18 RSODS-AODS-AMODS-AQODS-ALJ'sphere ODS-H80J'sphere ODS-M80J'sphere ODS-L80PolymerC18Triart C8Triart PhenylTriart PFPMeteoric Core C8Pro C8Pro C4C8
C4
TMSPhCNPROTEIN-RPYMCbasicYMC CarotenoidTriart Diol-HILICSILDiol-NPPolyamine ⅡNH2
PA-GPVA-SilBioPro SmartSep QBioPro SmartSep SBioPro QA-FBioPro SP-FBioPro QABioPro SPDiolCHIRAL ART Amylose-CCHIRAL ART Cellulose-CCHIRAL ART Amylose-SACHIRAL ART Cellulose-SBCHIRAL ART Cellulose-SCCHIRAL NEA (R)CHIRAL NEA (S)CHIRAL PREP CD STCHIRAL PREP CD PMCHIRAL α-CD BRCHIRAL β-CD BRCHIRAL γ-CD BR
TATARCASCAWASHSRSAAAMAQALJHJMJLPCTO
TPHTPFCOSOSBSOCBUTMPHCNPRBACT
TDHSLDNPBNHPGPVQSSSQFSFQAAPDL
KANKCNKSAKSBKSCNRNSSTPMDADBDG
5060801001201602003005001000proprietarynon-porous
050608101216203050A09900
sphericalirregular
SI
1.922.73456101520305075150
P902Q703040506111621305075A5
102030333550751001251502503005001000
010203H3H505L510R515253050A0
1.0 2.0 2.1 3.0 4.0 4.6 6.0 8.0 10 20 30 50 (1000 psi)50 (2000 psi)70 (1000 psi)70 (2000 psi)100(1000 psi)100(2000 psi)150(1000 psi)150(2000 psi)200(1000 psi)200(2000 psi)
0102Q1030446060810203051527172A1A2B1B2C1C2
Parker style (UPLC compatible)
Waters type
Cartridge
PTPTHWTWX
WTGWPGCGC
ExampleChoose your column and fill in the “Gel and Hardware Code” or detailed description
(The part number consists of the “Gel Code” and the “Hardware Code”).
Triart C18 120 Å spherical 1.9 µm 100 mm 2.0 mm Parker style
TA 12 S P9 10 02 PT
Your column part number: TA12SP9-1002PT (Example)
Please note that combinations of features cannot be selected at random, but only from the possible specifications for a chosen stationary phase. These can be determined from the individual product sections in this catalogue or from our homepage http://www.ymc.co.jp/en/
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