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DIRECCION GENERAL DE SANIDAD VEGETAL
DIRECCION DE MOSCAS DE LA FRUTA
IRRADIACION DE MOSCASDE LA FRUTA
EQUIPMENT ANDPROCEDURES
FRUIT FLY IRRADIATION
YEUDIEL GOMEZ SIMUTA July 2013
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1. PRINCIPLES
The gamma radiation i energ! in the "orm o"e#e$tromagneti$ %a&e %hi$h intera$t %ith matter 'rod($ingioni)ation
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The IONI*ATION$a(e igni+$ant$hange in thematter and in the$ae o" #i&ing
organim $a(e$hange in $e##,ti(e, organand the %ho#eindi&id(a#.
The t!'e and magnit(de o" DAMA-ES de'end on thet!'e o" radiation, it energ!, ASORED DOSE, thea/e$ted area and the e0'o(re time.
1. PRINCIPLES
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The ai#it! o" ioni)ing radiation to intera$t%ith the materia# i (ti#i)ed "or &ario(a''#i$ation
Ind(tr! Agri$(#t(re
Medi$a#Treatment
1. PRINCIPLES
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2. RADIATIONSOURCES
2.1 Gamma
Radiation
(rrent#! the mot %ide#! (ed gamma radiation "or the SIT i $oming34 Co and 156 C.
78
Co 34Co
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2.1.1
Radioisotopes
Sea#ed o(r$e
The o(r$e i
en$a'(#ated and$an not emani'(#ated
O'en o(r$eThe o(r$e $an e
mani'(#ated9N($#ear Medi$ine9
2. RADIATIONSOURCES
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2.2 electron
acceleratori'ment a#tho(gh ha&e not een %ide#! (ed, it i a 'otentia# te$hno#
"or "ood irradiation
2. RADIATIONSOURCES
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2.3 X Rays
; Ra! Ma$hine
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5. TEC>NOLO-Y
3.1 Gamma irradiators Se#"?Contained
The o(r$e i he#d in the a"et!'oition
-ama$e##224
The o(r$e @CS?156 andCo?34 i he#d in a a"et!
'oition 'rote$ted ! #eadhie#ding.
U(a##! ha a me$hanimto rotate and do%n the
am'#e to the irradiation
'oitionThee e:(i'ment $an'ro&ide high doe rate
(t ma## &o#(me o"irradiation.
idea# "or reear$h and ma##?$a#e 'rogram
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3.1 Gamma
Irradiators
Bo#(me 'er 'ro$e 5 #iter
-ama$e## 224@1
T!'i$a# Ditri(tion o"radiation o(r$e in a
-ama$e##.
Se#"?Contained
5. TEC>NOLO-Y
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3.1 Gamma
Irradiators PANORAMIC @1A
-amaeam
126
DRYSTORA-ESOURCE
The o(r$e $an e0it "rom thea"et! 'oition
CAPACITY 2.7 TON PER PROCESS
IRRADIACION PERATC>
5. TEC>NOLO-Y
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PANORAMIC @1A
CAPACITTY TON PER PROCESS
CONTINUOUS PROCESSIRRADIATION
S 644
GETSTORA-ESOURCE
The o(r$e $an e0it "rom thea"et! 'oition
5. TEC>NOLO-Y
3.1 Gamma
Irradiators
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3.2 X Rays Machines
Thee de&i$e are eing (ed in the SIT a an a#ternati&eo" n($#ear energ!
5. TEC>NOLO-Y
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3.2.1 Operation of a X RayMachine
rogramming irradiation doe
Loading
Container "or io#ogi$a# materia#
5. TEC>NOLO-Y
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3.2.2 X Ray Machine
Calibration
P#a$ing Fi#m and $ontainer'oition
-a"$hromi$ doimetr!!tem
5. TEC>NOLO-Y
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3.2.3 arameters and canister
arran!ement
Fig. 3. Canisterarrangement
5. TEC>NOLO-Y
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3.2." X Ray machine
operation #pdate
In 2411 COSTA RICA '(r$haed a ;?ra! e:(i'mentH in 2412egin %ith the o'eration o" ($h e:(i'ment "or ine$t teri#i)ing
5. TEC>NOLO-Y
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3.2 $ose $etermination by Gafchromic
$osimetry
DoeCa#iration
RADSOURCEe:(i'mentO" COSTA RICA
In 2412
Im'ortant
Parameter JB
mAThe doe i
$a#$(#ated !JG.e$
5. TEC>NOLO-Y
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3.3 %election of Irradiator
ai$ $om'onent o" an Irradiator
a RADIATION SOURCES @Radioioto'e, NOLO-Y
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3.3.1 &O'(%))
The 'o%er emitted ! a -amma?Ra! o(r$e $ontaining 144JCi o" 34Co i ro(gh#! e:(i&a#ent to that o" a 1.7 JGE#e$tron A$$e#erator @EA.
The 'o%er $a'a$it! o" $(rrent#! a&ai#a#e $ommer$ia#a$$e#erator %ith 7?14 MeB e#e$tron i ((a##! m($hgreater than thi, maJing them (n(ita#e "or dedi$atedSIT (e.
The eK$ien$! o" $on&erting e#e$tron into ; ra! i ao(t
6 "or 7 MeB e#e$tronH th( 85 o" the e#e$tron eam'o%er i NOLO-Y
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3." Radiolo!ical%afety
'he facility m#st to operate #nder a licenceemmited by the n#clear re!#latory a#thorityand m#st meet the basic sec#rityinternational re*#irements.
+lso the falicity m#st to ha,e incorporatedsafety interloc-s to a,oid accidental radiationepos#res and a,oid the access to#na#thori/ed personnel
5. TEC>NOLO-Y
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. STERILI*ATIONPROCEDURES
".1 Medici0n y distrib#ci0n de dosisabsorbida
".1.1 $eterminaci0nde dosis
DECAIMIENTORADIACTIBO
E# $on$e'to de &idamedia
ACTIBIDAD
Nmero de deintegra$ione :(e o$(rren en #a(nidad de tiem'o.
La a$ti&idad e #a ra'ide) de deintegra$inde #o n$#eo de (na m(etra de materia#radia$ti&o.
Contante de
de$aimiento
Personal Dosimetry
Sie&ert, (rem)
EXPOSURE
Intensity of
gammaradiation in Air
CJg ,(Roentgen
)
ASOREDDOSE -ra!,
(rad)
Becquerel = 1 desintegration/seconds (S.I.)
1 Curie = 3.7x1010desintegrations/sec
ACTIBITY:C(antit! o"radioa$ti&emateria#
e$:(ere#e,(C(rie)
Terminology
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".1 Meas#re and distrib#tion dose
".1.1 +cti,ity
ACTIBITY
N(mer o" deintegration on time "rom aradia$ti&e o(r$e
A = A0 e
tACTIBITY It i mea(re in e$:(ere#e,@C(rie
. STERILI*ATIONPROCEDURES
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".1.2 alf lifeRADIOACTIBE DECAY
>a#" #i"e $on$e't
The time re:(ired "or an! gi&en radioioto'e to de$a! to
one?ha#" o" origina# :(antit!
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
=
=
0.6932lnT1/2
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".1.3 alf life eha,ior
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
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".1." Radiolo!ical protectionprinciples
. AND T>EIR INFLUENCE IN T>E ASORED DOSE
DISTANCE? et%een the o(r$e and the organim
TIME? Time into the radiation +e#dS>IELDIN-.? et%een the radiation o(r$e and the organim
DISTANCIA
TIEMPO
LINDAE
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
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".1. $osecalc#lation
DOSIMETRY I the mea(rement o" radiation aoreddoe (ing doimeter.
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
DOSIMETER Materia# or de&i$e %hi$h $hange'ro'ortiona##! and re'rod($i#e d(e toradiation. The $hange i o"ten a 'h!i$a#'ro'ert! #iJe $o#o(r %hi$h i then :(anti+ed
ASORED I the amo(nt o" radiation energ! im'artedto the materia#DOSE o" interet. The SI (nit i -ra! @-!
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".1. $osecalc#lation
DOSIMETERS
PRIMARY DOSIMETER Ca#ormeter and Ioni)ation $hamerSTANDARD OR REFERENCE DOSIMETER Fri$Je , A#anineROUTINE DOSIMETER -a"$hromi$, O'ti$hromi$, et$
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
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".1.2 $oseMappin! -ama$e##?224
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
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TIPIA! DISTRI"UTIO# DOSE
-ammaeam? 126
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
".1.2 $oseMappin!
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RESULTS @ %et torageirradiator
$$% $$& $$& $0'
$$ $0 $$ $$&
$$ $& $0 $$*
$0 $$ $$ $$*
$$* $$0$$$$%
$& +y$0 +y
$$% +y
$$0 +y
MODELO S?644
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
".1.2 $oseMappin!
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; RAY
. STERILI*ATIONPROCEDURES
".1 Meas#re and distrib#tion dose
".1.2 $oseMappin!
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".2 Radiation dose for fr#it 4y
,or - capitata sterili.ation /as $*% +y($*-% ilorads) for standar strain (male and
females)-
A1t2ally for TS! Strain $00 and $% +y is2sed for sterili.ation
,or sterili.ation ofAnastrephaludensandAnastrepha obliqua 30 +y is 2sed -
And 4nally5 to a6oid ad2lt emergen1e ofA.ludens, a dose of *% to %0 +y is 2sed in
lar6ae stage-
. STERILI*ATIONPROCEDURES
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".3 Insect preparation
For man! ho#ometao#o( ine$t, the irradiation in the+na# 'ro$e o" '('a or ear#! ad(#t tage %a the mota''ro'riate
F#ie that ha&e een irradiated at ear#! '('a#
de&e#o'ment tend to e o" #o% :(a#it! @in term o"mating 'ro'enit!, !er, and 'rod($tion o"'heromone
The $o#oration o" e!e ha een one o" the etindi$ati&e and one o" the et too# to $a#$(#ated the
age o" the ine$t "or irradiation.
For e0am'#e te'hritid "r(it ie are irradiated at #eatt%o da! e"ore emergen$e a ad(#t and the io#ogi$a#materia# i e#e$ted ! e!e $o#or
. STERILI*ATIONPROCEDURES
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".". 5#ality ro!ram
".".1 Irradiator operation
Fo##o% the'ro'er
'ro$ed(rea$$ording to
theo'eration
man(a# andradiation
a"et!.
. STERILI*ATIONPROCEDURES
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$erti"! the
irradiation'ro$e$he$Jingthro(gh
radioeniti&e #ae#
that the'rod($tre$ei&ed thetarget doe.
".".2 5#ality certi6cation
. STERILI*ATIONPROCEDURES
".". 5#ality ro!ram
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".".2 Control rocess
%terility 'estRo#tine $osimetry7abel indicators
. STERILI*ATIONPROCEDURES
".". 5#ality ro!ram
7. FACTORS MODIFYIN-
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.1 Controlled+tmosphere
O0!gen #e&e#a/e$t the
eniti&it! o"
ine$t toradiation
The radiation?ind($eddamage i t!'i$a##!#o%er in red($ed
o0!gen atmo'herethan in air.
Free radi$a# "ormed $an e ne(tra#i)ed in the aen$e o"o0!gen to $omine %ith h!drogen radi$a#H %hi#e in the
'reen$e o" o0!gen "ree radi$a# $an "orm 'ero0ide andorgani$ mo#e$(#e $an e irre&eri#! a#tered.
INSECTRADIATION SENSITIBITY
.1 (n,ironmental and physicalfactors
7. FACTORS MODIFYIN-
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.1 (n,ironmental and physicalfactors
.2 $ose rate
Reference to %o#rce+cti,ity
.3 'emperat#re
%ome a#thors s#!!est that lo8 temperat#re increasesthe resistance of the insects.
INSECTRADIATION SENSITIBITY
7. FACTORS MODIFYIN-
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INSECTRADIATION SENSITIBITY
.2 iolo!ical 9actors
.2.1 Cel#lar'ransformation
Eggs
!ar6aeP27ae Ad2lt
0
%0
$00
RADIOSENSIILITY
Age
el2lar Transformation
Per$entage
RADIOSENSIILITYT8e radiosensi9ility is a f2n1tion of t8emeta9oli1 stage of t8e irradiated tiss2e-
7. FACTORS MODIFYIN-
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.2.2 7ife history :biolo!ical cycle; andcompleity of the or!anism
0-$
$
$0
$00
$000
$0000
Reiten$e
om7leity "iologi1al y1le
Doe @ rad
SUMMARY
The more$om'#e0 the
organim imore eniti&e
to radiation
horter #i"e
$!$#e o" theorganim
greaterreitan$e
INSECTRADIATION SENSITIBITY
.2 iolo!ical 9actors
3. RADIOLO-ICAL
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3. RADIOLO-ICALSAFETY
RADIATION SURBEY
SAFETY SYSTEM RECORD
GIPE TEST
MEDICAL SERBICE
@GORERS
CALIRATION EQUIPMENT
PERSONAL DOSIMETRY
DOSE RECORD @GORERS
SAFETY RADIOLO-YTRAININ-
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