1
Supplementary Information for
Xanthomonas translucens commandeers the host rate-limiting step in
ABA biosynthesis for disease susceptibility
Zhao Peng1, Ying Hu2, Junli Zhang1, Jose C. Huguet-Tapia1, Anna K. Block3, Sunghun
Park4, Suraj Sapkota5, Zhaohui Liu5, Sanzhen Liu2, Frank F. White1 1Department of Plant
Pathology, University of Florida, Gainesville, FL 32611, USA; 2Department of Plant
Pathology, Kansas State University, Manhattan, KS 66506, USA; 3 Center for Medical,
Agricultural and Veterinary Entomology, U.S. Department of Agriculture-Agricultural
Research Service, Gainesville, FL, 32608, USA; 4Department of Horticulture and Natural
Resources, Kansas State University, Manhattan, KS 66506, USA; 5Department of Plant
Pathology, North Dakota State University, Fargo, ND, USA
Correspondence to:
Email: [email protected]; [email protected]
This PDF file includes:
Supplementary text Figures S1 to S7 Tables S1 to S4 SI References
www.pnas.org/cgi/doi/10.1073/pnas.1911660116
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Supplementary Information Text
Plant Material, Plasmids, and Bacterial Strains.
Seeds of wheat cv. Jagger were provided by KSU Foundation Seed, and the cv. Chinese
Spring were from U.S. Department of Agriculture–Agricultural Research Service National
Small Grains Collection. Plants were grown in Metro-Mix 360 soil (Sungro) and
maintained in growth chamber. Growth condition was set at 24°C and 15-h light at day
time, and 22°C and 9-h dark at night with relative humidity at 75%. The bacterial strains
and plasmids used in this study are listed in Table S1. In brief, mutant strains of XT4699
were previously reported (1) and generated by homologous recombination of a mutant ORF
into the wild type copy using an antibiotic resistance gene maker for the recombinants. The
recombination was validated by PCR.
Virulence assays
Strains of X. translucens pv. undulosa (Xtu) were grown on tryptone sucrose agar (TSA)
for 2 days at 28°C and scraped from plates into sterilized distill water for inoculum with
OD600nm=0.2. The 2nd leaves of 10-day-old or 3rd leaves of 14-day-old wheat plants were
inoculated by needleless syringe infiltration to generate a water-soaking spot without
extension. The lesions were photographed and scored by measuring the lesion length at 7
days post inoculation (DPI). The humidity was set at 75% for standard virulence assays.
Chamber was set to 95% for high humidity infection and 50% for the control treatment.
Plants were grown in a Conviron PRG15 chamber with humidity control. Humidity was
monitored with a Lascar humidity and temperature logger to validate chamber controls.
For the bacterial growth population assay, the whole or sections of leaves were grounded
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in sterilized water using mortar and pestles. Serially diluted samples were plated onto
TSA plates. Colonies were counted three days after incubation of the plates at 28°C.
RNA-Seq analyses, prediction of candidate S genes for Tal8 and heatmap
constructions
Second leaves of 10-day-old wheat cultivars Jagger and Chinese Spring were infiltrated
with bacterial suspensions (OD600nm=0.5) by needleless syringe. 24 h after inoculation, 5
treated leaves were pooled for RNA extraction using RNeasy Plant Mini Kit (Qiagen).
RNA-seq libraries were prepared at Integrated Genomics Facility at Kansas State
University and sequenced at Genome Sequencing Facility at KU Medical Center. RNA-
Seq reads were subjected to adaptor and quality trimming using Trimmomatic software
(version 0.32) (2). RNA-seq data is deposited at NCBI Short Reads Archive (SRA)
database under project accession PRJNA485724. The remaining high-quality reads were
aligned to the International Wheat Genome Sequencing Consortium (IWGSC) flow-
sorted Chromosome Survey Sequence (CSS) contigs of Chinese Spring using STAR
version 2.5.0b. DESeq2 package were used to perform the differential expression analysis
(3-5) . Multiple test correction was accounted for by converting p-values to q-values (6).
Significantly differentially expressed genes (≥ 8-fold, p-value<0.01) in the highly up-
regulated genes of WT treated leaves from the cultivar Jagger compared to M6 were
selected and considered as candidate S genes. The promoter sequence, 500bp upstream of
translation start site, of all the candidate genes were searched for EBE of Tal8 by Target
finder based on its RVDs (7). The assumption was made that the unusual RVDs, KG, Y*,
and QD in Tal8 were functionally equivalent to NG, N*, and HD of the consensus or
common RVDs of other TALes. All the heatmaps were produced with heatmap.2
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function of the R package gplots using the log10 transformed reads per million reads
(RPM). All the genes annotated as homologs genes of wheat NPR1 were used for
generating heatmap (8). The wheat PR1 genes used for generating heatmap of TaPR1
were previously reported (9).
Sequence analysis of additional low and high virulence strains of Xtu
Genome sequencing was conducted using SMRT-Pacbio technology. Raw reads were
assembled using Canu software v1.5 (10). Assemblies were polished using the genomic
consensus package with the arrow algorithmic
(https://github.com/PacificBiosciences/GenomicConsensus). Genome comparisons and
alignments were conducted using mummer4 (11). TAL effector sequences analysis was
conducted using QueTALE software (12). Assembled genomes were submitted to the
Genbank with accession numbers: CP043540 for Xtu LW16 and CP043500 for Xtu P3.
dTALe construction and transformation
The promoter of the Tal6 effector in XT4699, which was previously shown to drive gene
expression in wheat, was used for driving the expression of dTALes in strains of Xtu (1) .
The repeat regions of dTALes were constructed using Golden Gate assembly strategies
(13,14). The dTALes driven by tal6 promoter were individually transferred into a broad
host-range vector pHM1 by restriction enzymes. The final constructs were sequenced and
transformed into M6 or LW16 strains by electroporation (Bio-rad).
Quantitative RT-PCR (qRT-PCR) analyses
Xtu strains harboring dTALes were validated by qRT-PCR analyses of targeting host
genes. The leaves were inoculated with Xtu at OD600nm=0.2 and were harvested at 1DPI
for RNA isolation (Trizol reagent, Invitrogen). 1μg total RNA was treated with DNaseI
5
(Invitrogen) first and applied for reverse transcription reaction (Verso cDNA Synthesis
Kit). The 10X diluted cDNA samples were subjected to real-time PCR reactions in the
96-well CFX machine (Bio-rad). The wheat EF-1α gene was used as the control and the
relative gene expression level was calculated using 2-∆∆Ct method (15). Primer sequences
are available in Table S2.
Transient GUS assay in Nicotiana benthamiana
Primers (Table S2) for the predicted EBETal8 of TaNCED_5BS and TaNCED_5DS were
annealed and ligated into upstream of CsLobT. The synthetic promoters were cloned into
pBI101 (Clonetech) to fuse upstream of uidA. Approximately 500 bp of native promoters
of TaNCED_5S were PCR amplified using specific primers (Table S2) and fused with
uidA. The constructs were individually transformed into Agrobacterium LBA4404. The
full length tal8 was PCR amplified using primers (Table S2), fused under 35S promoter
in pBICaMV (16), and then transformed into Agrobacterium strain LBA4404. 1:1 ratio
mixture of Agrobacterium (OD600nm=0.3) with designated promoter::uidA and tal8 was
infiltrated onto young leaves of 1-month old N. benthamiana plants by needleless
syringe. The N. benthamiana plants were maintained at 24°C with 12-h photoperiod in
the lab room. Leaf discs of inoculated areas were punched and collected 3 days later to
test the GUS activity in qualitative and quantitative manners as described (16).
ABA quantification assay
The 2nd leaves of 11-day-old Jagger plants were infiltrated with bacterial suspensions
(OD600nm=0.2) of X. translucens strains by needleless syringe. The inoculated leaves were
collected at 1 DPI, weighed at scale, frozen with liquid nitrogen and stored at -80°C. The
endogenous ABA was extracted following the protocol as described (17). The
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quantification of ABA was performed with Phytodetek ABA Test Kit following
directions of the manufacturer.
Application of ABA and effect of Tal8 in wheat leaves
The 10-day-old or 14-day-old Jagger plants were sprayed with ABA (100µM) coated
with 0.02 % Silwet L–77. The water-soaking streak phenomenon induced by the sprayed
ABA was observed in one day. At this time, 2nd leaves of 10-day-old or 3rd leaves of 14-
day-old ABA treated plants and control plants (sprayed without ABA) were infiltrated
with bacterial suspension (OD600nm=0.2) to form a water-soaking spot by needleless
syringe. The symptoms were photographed and scored by measuring the lesion length at
7 DPI. The 2nd leaves of 12-day-old Jagger plants were inoculated by bacterial suspension
(OD600nm=0.2) and at 1 DPI the inoculated leaves were placed in the middle of chamber
of LI-COR 6800 for measurement following the manual. GasEx E values were recorded
to determine the transpiration rate. For water loss rate measurement, four infiltrated 2nd
leaves of 12-day-old Jagger at 1DPI were excised, pooled and weighed at time points of
0, 20, 40, 60, 120, 180min. The water loss rate was calculated as percentage of initial
fresh weight (18).
SA measurement and application
The 2nd leaves of 12-day-old Jagger plants were inoculated by X. translucens pv.
undulosa strains (OD600nm=0.2). The inoculated leaves were sampled at 1 DPI, 2 DPI and
3 DPI. To quantify total and free SA a GC-MS method was adapted from Schmelz et.al.,
2004 (19). Briefly tissues were flash frozen in liquid nitrogen and then c.a. 100mg
extracted in 600µl of H2O:1-propanol:HCL (1:2:0.005) spiked with 200ng of d4-SA as an
internal standard. Samples were homogenized using 1g of ceramic beads and partitioned
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into two equal samples one for free SA and one for total SA. For total SA 2µl of glacial
HCL was added to the sample, and the sample was boiled for 30 min in a water bath and
allowed to cool to 25 °C. Both free and total SA samples were then extracted with 1ml
MeCl2. Following centrifugation, the MeCl2:1-propanol layer was collected and
derivatized with trimethylsilydiazomethane, quenched with acetic acid in hexane and
compounds collected by vapor phase extraction on SuperQ columns. Compounds were
then eluted in MeCl2 and analyzed using GC-MS. One day before inoculation, the 1mM
SA solution coated with 0.02 % Silwet L–77 were sprayed onto leaves or the plant soil
was thoroughly soaked by 1mM SA solution. The 2nd leaves of 10-day-old Jagger were
used for inoculation.
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Fig. S1
9
Fig. S1. Tal8 is a virulence effector for XT4699. (A and B) M6 mutant shows loss of
virulence compared to wild type XT4699, complemented M6 [M6(tal8)], and mutants
M1-5, M7, and M8 on wheat cultivars cv. Jagger (A) and cv. Chinese Spring(B) as
measured by lesion length. *** indicates reduced virulence of M6 (P-value < 0.001)
compared to XT4699 with Student’s t-test. (C) Representative leaf inoculations on cv.
Chinese Spring at 7DPI with strains indicated above panel. (D and E) Total and surface
bacterial populations were not measurably different between XT4699, M6(tal8) and M6
on cv. Jagger (D) or cv. Chinese Spring (E).
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Fig. S2
Fig. S2. Tal8 amino acid sequence and repeat variable di-amino acid (RVD)
residues. (A) Tal8 amino acid sequence from NCBI accession AKK68514. The repeat-di-
residues (RVD) of each repeat are in bolded font. (B) Single letter code for the RVD of
Tal8 effector. The lowercase letters g and d were used for indication of unusual di-amino
acid residues KG and QD at the 12th and 13th positions. The RVDs KG, QD, and Y*
were treated as equivalent to NG, HD, and N* for purposes of EBE analysis.
>Tal8 NCBI Accession AKK68514, locus tag FD63_14025 CP008714 REGION:
complement(3256565..3260576) MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSDLLRQ
VDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRAAQTSDASP
AADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSEMIAALPEATHED
IVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGAPLH LTPDQVVAIVSNNGGKQALETVQRLLPVLCKPPYG LTPEQVVAIASNGGGKPALETVQRLLPVLCQEYG LTPNQVVAIASHDGAKQALETVQRLLPVLCQPPHP LTPNQVVAIASHDGGKPALETVQRLLPVLCKPPYG LTPEQVVAIANHDGAKQALETVQRLLPVLCQPPYG LTPEQVVAIASKGGGKQALETVQRLLPVLCQPPYG LTPDQVVTIASNNGGKPALETVRRLLPVLCKPPYG LTPKQVVAIASY*GGKQSLETVQRLLPVLCKPPYG LTPEQVVAIASNGGGKPALETVQRLLPVLCQEYG LTPNQVVAIASHDGAKPALETVQRLLPVLCQEYG LTPNQVVAIASHDGAKQALETVQRLLPVLCKPPYG LTPEQVVAIASQDGGKQSLETVQRLLPVLCKPPYG LTPEQVVAIASHNGGTQALESIFAQLSSPDPALAALTNGRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVP
EGTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQ
ASGRKGAKPSSASAQTPSQESVDAFADSLERELDAHSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVFVPEQ
RDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
A
Single letter code for RVD of Xtu 4699 Tal8 NN NG HD HD HD KG NN Y* HG HD HD QD HN N G D D D g N * G D D d N
B
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Fig. S3
12
Fig. S3. Promoter sequences and predicted EBETal8 of TaNCED and TaERF. (A) The
predicted alignments are shown for RVDs of Tal8 and EBEs of TaNCED_5BS,
TaNCED_5DS and TaNCED_5AS. Nonconsensus nucleotide in the RVD pairings are
highlighted in yellow. The alignment of EBEs for all three genes is shown above the
sequences for the respective promoter regions. The start codon ATG is highlighted in
green. Concensus nucleotide matches in EBEs are highlighted in turquoise. The putative
TATA box is underlined. (B) The alignment of RVD of Tal8 with EBEs in the promoter
regions of TaERF_1BL, TaERF_1DL and TaERF_1AL. Highlighting as in A.
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Fig. S4
Fig. S4. Sequence analysis of X. translucens pv. undulosa strains P3 and LW16. (A)
TAL effector genes (arrow) are located in same positions of the genomes in LW16, P3,
and XT4699. (B) Dot plot analysis of LW16 (y-axis) versus P3 (x-axis). Red dots
represent forward alignments. Blue dots represent reverse alignments (inversions).
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
4000000
4500000
0 500000 1000000 1500000 2000000 2500000 3000000 3500000 4000000 4500000
lw16r1
tig00000001|arrow|join_near_2309204|pilon
P3
Lw1
6
B
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8
LW16
P3
A
1 2 3 4 5 6 7 8 XT4699
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Fig. S5
Fig. S5. Alignment of RVDs of TAL effectors of XT4699, LW16 and P3 strains of X.
translucens pv. undulosa. LW16-Tal8 RVDs are marked with red and do not align with
RVDs from Tal8 of P3 and XT4699. Other TAL effectors were highly similar among
three strains except variable RVDs, which were marked with purple highlight. The
missing 13th amino acid in the repeat was represented by *.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 XT4699-Tal1 HD YD NI NG NG NN YK NG HD NG NG ND NG QD NH HD P3-Tal1 HD YD NI NG NG NN YK NG HD NG NG ND NG QD NH HD LW16-Tal1 HD YD NI NG NG NN YK NG HD NG NG ND NG QD NH QD XT4699-Tal2 NN HD NG NN HN KG NI HD NI NN HD HN HD HD NI HN HD QD P3-Tal2 NN HD NG NN HN KG NI HD NI NN HD HN HD HD NI HN HD QD LW16-Tal2 NN HD NG NN HN KG NI HD NI HN HD HN HD HD NI HN HN HD XT4699-Tal3 NN HD NG HD HD HN NF NI NH HD HD HD HN HN HD P3-Tal3 NN HD NG HD HD HN NF NI NN NN HD HD HN HN HD LW16-Tal3 NN HD KG HD HD HN NF NI NN HD HD HD HN HN HD XT4699-Tal4 NH NN HD NN HD NH HD YK NG NH Y* HD NN NI NG QD P3-Tal4 NH NN HD NN HD NH HD YK NG NH Y* HD NN NI NG QD LW16-Tal4 NH NN HD NN HD NH HD YK NG NH Y* HD NN NI NG QD XT4699-Tal5 NN HD NG NN HN HN NI NI NI NH NN HD NN NH HD HD P3-Tal5 NN HD NG NN HN HN NI NI NI NH NN HD NN NH HD HD LW16-Tal5 NN HD NG NN HN HN NN NI NI NH NN HD NN NH HD HD XT4699-Tal6 HD HN HN HD NH NH HG HD KG NN Y* NG HD HD HN P3-Tal6 HD HN HN HD NH NH HG HD KG NN Y* NG HD HD HN LW16-Tal6 HD HN HN HD NH NH HG HD KG NN Y* NG HD HD HN XT4699-Tal7 HN HD HD HD NI NI NI HN HD HD NN NN NI NN HD P3-Tal7 HN HD HD HD NI NI NI HN HD HD NN NN NI NN HD LW16-Tal7 NN HD HD HD NI NI NI HN HD HD NN NN NI NN HD XT4699-Tal8 NN NG HD HD HD KG NN Y* NG HD HD QD HN P3-Tal8 NN NG HD HD HD KG NN Y* NG HD HD QD HN LW16-Tal8 NN HD NG NN HN NG NI HD NI NN HD HD NN NN NI HN HD
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Fig. S6
Fig. S6 TaNCED_5BS expression is associated with enhanced virulence of M6. (A)
Empty vector (ev) and dTALes, dERF101 and dNCED63, designed specifically for
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TaERF_1BL and TaNCED_5BS, were transformed into the tal8 mutant strain M6.
M6(dERF1011) and M6(dERF1012) are two independent transformants of dERF101.
qRT-PCR results revealed the specific inductions of gene by corresponding dTALes. (B)
Lesion length measurements at 7 DPI showed only the dNCED63 transformants
enhanced the virulence for M6 on the 2nd leaves of 10-day-old Jagger plants. *** -
indicates values differ in the significance at the p-value < 0.001 relative to lesion lengths
of M6(ev) with Student’s t test. ns – values non-significant difference relative to lesion
lengths of M6(ev).
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Fig. S7
18
Fig. S7. Phylogenetic analysis of NCED and presence and effects of salicylic acid in
wheat leaves after infection. (A) Protein sequences of NCED from Arabidopsis, rice,
maize and wheats were analyzed and aligned. Phylogenetic tree was generated by
Neighbor-joining method in the Geneious software with AtCCD1 sequence as outgroup.
Upregulated wheat genes are indicated by red line. Genbank accession numbers of
proteins are: AtCCD1 (NP_191911),AtNCED2 (NP_193569), AtNCED3(NP_188062),
AtNCED4(NP_193652), AtNCED5(NP_174302), AtNCED6(NP_189064),
AtNCED9(NP_177960), OsNCED1(XP_015626662),
OsNCED2(XP_015619611),OsNCED3(XP_015631538), OsNCED4(XP_015645858),
OsNCED5(XP_015618707), ZmVP14(AAB62181). Protein sequences are provided in
Table S4. (B and C) Free SA (B)and total SA (C) from inoculated wheat leaf samples
were quantified by GC-MS. Bacterial strains LW16(ev), LW16(dNCED63) and
LW16(tal8) were inoculated onto 2nd leaves of 12-day-old Jagger. Inoculated samples
with bacterial suspensions (OD600nm=0.2) were harvested at 1 DPI, 2 PDI and 3 DPI.
Values indicate mean ± standard error. Values with the same lowercase letters do not
differ significantly (p-value < 0.05) using Tukey statistics and ANOVA analysis. Details
for SA measurements are described in Methods and Materials. (D and E) 9-day-old
Jagger plants were sprayed (D) with 1mM SA or soil-drenched (E) with 1mM SA,
respectively. Control plants were not treated with SA. The 2nd leaves of Jagger plants
were inoculated one day after treatment. Each bar signifies the range of mean ± standard
deviation. The lower-case letters show significantly different groups (p-value < 0.05) in
the Tukey statistic and ANOVA analysis.
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Table S1
Table S1. Bacterial strains and plasmids used in this study.
Xtu strains Notes
XT4699 WT strain (2)
LW16 WT strain (2)
XT130 WT strain(2)
XT5523 WT strain (2)
XT5770 WT strain (2)
XT5791 WT strain (2)
LB10 WT strain (2)
LG48 WT strain (2)
P3 WT strain (2) M1 XT4699 tal2 mutant (2)
M2 XT4699 tal6 mutant (2)
M3 XT4699 tal7 mutant (2)
M4 XT4699 tal1 mutant (2)
M5 XT4699 tal5 mutant (2)
M6 XT4699 tal8 mutant (2)
M7 XT4699 tal3 mutant (2)
M8 XT4699 tal4 mutant (2)
M6 (ev) M6 strain harboring empty broad host range cloning vector pHM1
M6 (tal8) M6 strain harboring tal8 in pHM1
M6 (dNCED63) M6 harboring dNCED63 in the pHM1 M6 (dERF1011) harboring dERF101-1 in pHM1, independent transformant of M6 M6 (dERF1012) harboring dERF101 -2 in pHM1, independent transformant of M6 LW16 (ev) LW16 harboring empty vector pHM1
LW16 (tal8) LW16 harboring tal8 gene in pHM1
LW16 (dNCED41) LW16 harboring dNCED41 in pHM1
LW16 (dNCED53) LW16 harboring dNCED53 in pHM1
LW16 (dNCED63) LW16 harboring dNCED63 in pHM1
LW16 (dNCED72) LW16 harboring dNCED72 in pHM1
LW16 (dERF3) LW16 harboring dERF3 in pHM1
LW16 (dERF4) LW16 harboring dERF4 in pHM1
LW16 (dERF6) LW16 harboring dERF6 in pHM1
LW16 (dERF101) LW16 harboring dERF101 in pHM1
Agrobacterium
LBA4404 WT strain from Dr. Sunghun Park at Kansas State University
LBA4404 (tal8) LBA4404 strain with tal8 coding sequence fused to 35S CaMV promoter
LBA4404 (pthA4) LBA4404 strain with overexpressed pthA4 (Hu et al., 2014)
LBA4404 (NCEDB) LBA4404 strain with native promoter of TaNCED_5BS fused upstream of uidA
gene in the pBI101
LBA4404 (NCEDD) LBA4404 strain with native promoter of TaNCED_5DS fused upstream of uidA
gene in pBI101
LBA4404 (CsLob) LBA4404 strain with native promoter CsLob1 fused upstream of uidA gene in
pBI101
LBA4404 (CsLobT) LBA4404 strain with truncated promoter CsLobT fused upstream of uidA gene
in pBI101
LBA4404 (EBEB-
CsLobT)
LBA4404 strain with synthetic promoters consisting of EBETal8 of TaNCED_5BS
placed upstream of CsLobT, and then fused upstream of uidA gene in pBI101
LBA4404 (EBED-
CsLobT)
LBA4404 strain with synthetic promoters consisting of EBETal8 of
TaNCED_5DS placed upstream of CsLobT, and tfused upstream of uidA gene in
pBI101
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Plasmids
pHM1 Broad host-range vector for Gram-negative bacteria (White lab)
pBICaMv Modified vector from pBI121, suitable for overexpressing genes under CaMV
35S promotor (Hu et al., 2015)
pBI101 Clonetech vector
pBI101-CsLobT Truncated CsLob1 promoter inserted upstream of uidA gene in the pBI101 vector
(Hu et al., 2014)
Peng, Z. et al., (2016) BMC Genomics, 17, 21.
Hu, Y. et al., (2014) Proc Natl Acad Sci U S A, 111, E521-9.
Hu, Y. et al., (2015) Horticulture Research, 2.
21
Table S2
Table S2. Primers used in this study. Primers used in qRT-PCR
TaNCED-qF1 TCAAGAAGCCGTACCTCAAG
TaNCED-qR1 ACGAAGTTCTCGGTGATGG
TaERF-qF ATGGCGACAATAACGACAAC
TaERF-qR CATACTCCTGGTTCTTGAATGG
TaEF-1α-qF1 CAGATTGGCAACGGCTACG
TaEF-1α-qR1 CGGACAGCAAAACGACCAAG
TaNPR1-F GAGATGCCTGGAGATAGTAGTC
TaNPR1-R CCAAGAGTTATCCGTGAATCAAT
Primers used in transient GUS assay
NCED-pF1 AATCGCATGCCCCCCACATGAGCATACATA
NCED-pF2 AATCGCATGCGAAAGAAAAGAAGAGTGGAGAG
NCED-pR ATCATCTAGAGCTGATCCTYGGAAGAAGAA
NCED5B-FOR AGCTCTGTCCCTTCTCCCCCCCTCCCAAA
NCED5B-REV CTAGTTTGGGAGGGGGGGAGAAGGGACAG
NCED5D-FOR AGCTCTGTCCCGTCTCCCCCCCTCCCAAA
NCED5D-REV CTAGTTTGGGAGGGGGGGAGACGGGACAG
Tal8-OX-F actaTCTAGAATGGATCCCATTCGTTCTCG
Tal8-OX-R atgaGAATTCTCAATGAGCCAATAGCTCCTTC
22
Table S3
Table S3. TAL effectors of Xtu P3 (high virulence) and LW16 (low virulence)a
P3-Tal1
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASGPAFSAGSFSD
LLRQVDSSLFNASLFDSMPAFGAHHAQAATGELDEAQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQ
TLDALPAADVDISTFGYSQQQQEKIKPKVRSTVAQHHAALVGHGFTHAHIVELSKHPPALGTIAARYSEMI
AALPEATHEDIVGVGKHCAGARTLEVLLMVVQELRAPPLQLVTSQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLLPDQVVAIVSHDGGKQSLETVQRLLPVLRQPPYGLTPNQVVAIASYDGGKQSLETVERLLPVLCQPP
YGLTPNQVVAIASNIGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASNGGGKQALETVQRLLPVLCQEYG
LIPEQVVAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPPYGLT
PKQVVAIASYKGANQALGTVQRLLPVLCKPPYGLTPDQVVAIASNGGAKQALETVERLLPVLCKPPYGLTP
DQVVAIASHDGGRQALETVQRLLPVLCQPPYGLTPNQVVAIASNGGGKQALETVQRLLPVLCQEYGLTRQQ
VVAIASNGGGKQSLETVQRLLPVLCKPPYNLTPDQVVAIASNDGGKQALETVQRLLPVLCKPPYNLTPEQV
VAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASQDGGKQSLETVQRLLPVLCQLPYGLTPNQVVA
IASNHGGKQSLETVQRLLPVLCQPPYGLTPNQVVAIASHDGGTQALESIFAQLSSPDPALAALTNDRLVAL
ACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADHAQVVRVLSFFQCHSQRGQVFHEAMKRFEMS
REGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAP
SPMHQAGQALASSRKRSRSASSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTH
GDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
P3-Tal2
MDPIRSRTPSPARELQAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSSVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASHDGAKPALETVQRLLPVLCQ
PPYGLTPEQVVAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPP
YGLTPKQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASKGGGKQALETVQRLLPVLCQPPY
GLTPDQVVTIANNIGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHDGGKQALETVQRLLPVLCQEYGL
TPGQVVAIANNIGGKPALETVQRLLPVLCQPPYNLTPNQVVAIASNNGGKQALETVQRLLPVLCKPPHPLT
PNQVVAIASHDGAKQALETVQRLLPVLCKSPYGLTPDQVVVIASHNGGKQALETVQRLLPVLCKPPYGLTP
EQVVAIASHDGGKPALETVQRLLPVLCQEYGLTPNQVVAIASHDGGKQALETVQRLLPVLCQDHGLTPGQV
VAIANNIGGKPALETVQRLLPVLCKEYGLTPNQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTPEQVVA
IASHDGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASQDGGTQALESIFAQLSSPDPALAALTNDRLVAL
ACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADHAQVVRVLSFFQCHSQRGQVFHEAMKRFEMS
REGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAP
SPMHQAGQTLASNRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTH
GDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
P3-Tal3
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASGPSFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEAQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQ
TSDALPAADVDLSTFGYSQQQQEKIKPKVRSTVAQHHAALVGHGFTHAHIVELSKHPPALGTIAARYSEMI
AALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCQPPYNLTPEQVVAIASHDGAKPALETVQRLLPVLCQPP
YGLTPEQVVAIASNGGKQALEAVQRLLPVLCKEYGLTPEQVVAIASHDGAKQALETVQRLLLVLCQEYGLT
PNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTP
EQVVTIANNFGGKPALETVQRLLPVLCQPPYGLTPNQVVAIANNIGAKPALETVQRLLPVLCQPPYGLTPE
QVVAIAGNNGAKQALETVQRLLPVLCQPPYGLTPEQVVAIAGNNGAKQALETVQRLLPVLCQPPHPLTPNQ
VVAIASHDGAKQALETVQRLLPVLCKPPYGLTPDQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTPDQV
VAIASHNGGKQALETVQRLLPVLCQPSYGLTRNQVVAIASHNGGKQALETVQRLLPVLCKEYGLTPEQVVA
IASHDGGTQALESIFAQLSSPDPALAALTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTA
HLVADHAQVVRVLSFFQCHSQRGQVFHEAMKRFEMSREGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQ
ASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVF
23
VPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADSFAGAAEDFPAFDQEEIAW
LKELLAH
P3-Tal4
MEPIRSRTPSTARELQAGSQPDAVQPIADRLVSTPASSPLDGLPARRMVSRTSPPPSPARSPAFSAGSLSG
LLRQQIDPSLFAGSPFDSLPSFGAARAESAPGEGDEVQSGLRAVDDPQPSASAAITAAPPRTKAAARRRSA
QTSDALPAAHVDLGTFGYSQQQQEKIKPKVRSTLAQHHEALVGHGFTHAHIVELSKHPPALGTIAARYSEM
IAALPEATHEDIVGVGKQKSGARALEALLTVAEELRAPPLQLVTGQLLKIAKRGGVNAVEAVHASRNALTG
APLHLLPDQVVAIVSNHGSKLALGTVQRLLPVLCKPPYGLTRNQVVAIVNNNGGKQALETVHRLLPVLCQP
PYGLTPEQVVAIASHDGGRQALETVHRLLPVLRQPPYGLTLEQVVAIASNNGGKQALETVQRLLPVLCQPP
YGLTPDQVVTIASHDGGRQALETVQRLLPVLCQPPYGLTPNQVVAIASNHGGKQALETVQRLLPVLCQPLH
GLTPDQVVTIASHDGGRQALETVQRLLPVLCQPPYGLTPKQVVAIANYKGAKQALETVQRLLPLLCKPPYG
LTPDQVIAIVSNGGGKPALETVRRLLPVLCKHPYGLTPKQVVAIVSNHGGKPALETVRRLLPVLCEHPYGL
TPKQVVAIASYGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASHDGGRQALETVQRLLPVLRQPPYGLTP
DQVVAIASNNGGKQALETVQRLLPVLRQPPYSLIPDQVVAIASNIGAKQSLETVQRLLPVLCQPPYGLTTD
QVIAIASNGGSKQALETVQRLLPVLCQPPYGLTPDQVVAIASQDGGKQALESIFAQLSSPDPALAALTNDR
LVALACLGGRPALDAVKKGLPHAPALVTRTHNRVPEGTAHLVADHAQVVRVLGFFQCHSHPAQAFHEAMTR
FEMSREGLLQLFRRVGVTELEAISGTLPPASQRWHRILQALGVKGAKPSSASAQTPSQESVDAFADSLERE
LDAPSPIHEADRARASNRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRQRTRIGGLPDPG
MPTDGELAASSAAFLEQDADPFAGAAEDFPVFDQEEIAWLMTLLPH
P3-Tal5
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPPALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSNNGGKQALETVERLLPVLCQPPYGLTPEQVVAIASHDGAKPALETVQRLLPVLCQ
PPYGLTPEQVVAIASNGGGKPALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPP
YGLTPNQVVAIASHNGGKQSLETVQRLLPVLCQPPYGLTPEQVVAIASHNGGKQALETVQRLLPVLCQPPY
GLTPEQVVTIANNIGGKQALETVERLLPVLCQPPYGLTPNQVVAIASNIGAKPALETVQRLLPVLCQPPYG
LTPEQVVTIANNIGGKPALETVQRLLPVLRKPPYGLTPEQVVAIASNHGGKQALETVQRLLPVLCKPPYGL
TPNQVVAIASNNGAKQALETVQRLLPVLCKPPHPLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLT
PEQVVVIASNNGGKQALETVQRLLPVLCQPSYGLTPEQVVAIASNHGGKQALETVQRLLPVLCQPPYGLTP
NQVVAIASHDGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASHDGGTQALESIFAQLSSPDPALAALTND
RLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMR
QFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQASGRKGAKPSSASAQTPSQESVDAFADSLER
ELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDP
GTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
P3-Tal6
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALETLLMVAEELRAPPLQLVTGQLLKIAKGGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSHDGGKQSLETVQRLLPVLCQPPYGLTPNQVVAIASHNGGKQALETVQRLLPVLCQ
EYGLTPEQVVAIASHNGGKQALETVQRLLPVLCQEYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPY
GLTPEQVVAIASNHGGKLALETVERLLPVLCQPPYGLTPNQVVAIASNHGGKQALETVQPLLPVLCQPPYG
LTPEQVVAIASHGGAKQALKTVQRLLPVLCQDHGLTPEQVVAIANHDGAKQALETVQRLLPVLCQPPYGLT
PEQVVAIASKGGGKQALETVQRLLPVLCQPPYGLTPDQVVTIASNNGGKPALETVRRLLPVLCKPPYGLTP
KQVVAIASYGGKQALETVQRLLPVLCKPPYGLTPEQVVAIASNGGGKPALETVQRLLPVLCQEYGLTPNQV
VAIASHDGAKPALETVQRLLPVLCQEYGLTPNQVVAIASHDGGKPALETVQRLLPVLCQPPYGLTPEQVVA
IASHNGGTQALESIFAQLSSPDPALAALTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTA
HLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQ
ASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVF
VPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAW
LKELLAH
24
P3-Tal7
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPSASGPAFSAGSFSD
LLRQVDSSLFNASLFDSMPAFGAHHAQAATGELDEAQSALRAVDDPQSSASAAITAAPPRTKAAARRRSAQ
TLDASPAADVDLSTFGYSQQQQEKIKPTVRSSVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSEMI
AALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLTPDQVVAIVSHNGGKQALETVQRLLPVLCQPPYNLTPEQVVAIASHDGGKQALETVQRLLLVLCQEC
GLTPNQVVAIASHDGAKPALETVHRLLPVLCQPPYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQDHGL
TPGQVVAIADNIGGKQALETVQRLLPVLCKPPYGLTPEQVVTIANNIGGKPALETVQRLLPVLCQPPYGLT
PEQVVTIANNIGAKPALETVHRLLPVLRKPPYGLTPEQVVAIASHNGAKPALETVQRLLPVLCQPPYGLTP
NQVVAIASHDGAKQALETVQRLLPVLCKPPHPLTPNQVVAIASHDGAKQALETVQRLLPVLCKPPYGLTPD
QVVVIASNNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASNNGGKPALETVQRLLPVLCKPPYDLTPDQ
VVAIANNIGAKPALETVQRLLPVLCQPPYGLTPNQVVAIASNNGGKQALETVQRLLPVLCQPPYGLTPYQV
VAIASHDGGTQALESIFAQLSSPDPALATLTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPEG
TAHLVADHAQVVRVLGFFQCHSQRGQVFHEAMKRFEMSREGLLQLFRRVGVTELEAISGTLPPASQRWDRM
LQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAE
VFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGMPTDGELAASSAAFCEQDADPFAGAAEDFPAFDQEEI
AWLRELLAH
P3-Tal8
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCKPPYGLTPEQVVAIASNGGGKPALETVQRLLPVLCQ
EYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPHPLTPNQVVAIASHDGGKPALETVQRLLPVLCKPP
YGLTPEQVVAIANHDGAKQALETVQRLLPVLCQPPYGLTPEQVVAIASKGGGKQALETVQRLLPVLCQPPY
GLTPDQVVTIASNNGGKPALETVRRLLPVLCKPPYGLTPKQVVAIASYGGKQSLETVQRLLPVLCKPPYGL
TPEQVVAIASNGGGKPALETVQRLLPVLCQEYGLTPNQVVAIASHDGAKPALETVQRLLPVLCQEYGLTPN
QVVAIASHDGGKPALETVQRLLPVLCKPPYGLTPEQVVAIASQDGGKQSLETVQRLLPVLCKPPYGLTPEQ
VVAIASHNGGTQALESIFAQLSSPDPALAALTNGRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPE
GTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDR
MLQASGRKGAKPSSASAQTPSQESVDAFADSLERELDAHSPMHQAGQTLASSRKRSRSESSVNRSSAQQAA
EVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQDE
LAWLKELLAH
LW16-Tal1
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASGPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEAQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQ
TLDALPAADVDLSTFGYSQQQQEKIKPKVRSTVAQHHAALVGHGFTHAHIVELSKHPPALGTIAARYSEMI
AALPEATHEDIVGVGKHCAGARTLEVLLMVVQELRAPPLQLVTSQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLLPDQVVAIVSHDGGKQSLETVQRLLPVLRQPPYGLTPNQVVAIASYDGGKQSLETVERLLPVLCQPP
YGLTPNQVVAIASNIGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASNGGGKQALETVQRLLPVLCQEYG
LIPEQVVAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPPYGMT
PKQVVAIASYKGANQALGTVQRLLPVLCKPPYGLTPDQVVAIASNGGAKQALETVERLLPVLCKPPYGLTP
DQVVAIASHDGGRQALETVQRLLPVLCQPPYGLTPNQVVAIASNGGGKQALETVQRLLPVLCQEYGLTRQQ
VVAIASNGGGKQSLETVQRLLPVLCKPPYNLTPDQVVAIASNDGGKQALETVQRLLPVLCKPPYNLTPEQV
VAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASQDGGKQSLETVQRLLPVLCQLPYGLTPNQVVA
IASNHGGKQSLETVQRLLPVLCQPPYGLTPNQVVAIASQDGGKQALESIFAQLSSPDPALAALTNDRLVAL
ACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMS
RHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAP
SPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTH
GDLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
LW16-Tal2
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASGPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEAQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQ
TLDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSEMI
25
AALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLTPDQVVAIVSNNGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASHDGAKPALETVQRLLPVLCQPP
YGLTPEQVVAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPPYG
LTPKQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASKGGGKQALETVQRLLPVLCQPPYGL
TPDQVVTIANNIGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQEYGLTP
GQVVAIANNIGGKPALETVQRLLPVLCQPPYNLTPNQVVAIASHNGGKQALETVQRLLPVLCKPPHPLTPN
QVVAIASHDGAKQALETVQRLLPVLCKSPYGLTPDQVVVIASHNGGKQALETVQRLLPVLCKPPYGLTPNQ
VVAIASHDGGKPALETVQRLLPVLCQPPYGLTPDQVVAIASHDGGKQALETVQRLLPVLCQDHGLTPGQVV
AIANNIGGKPALETVQRLLPVLCKPPYGLTPDQVVAIASHNGGKQALETVQRLLPVLCQPSYGLTRNQVVA
IASHNGGKQALETVQRLLPVLCKEYGLTPEQVVAIASHDGGTQALESIFAQLSSPDPALAALTNDRLVALA
CIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADHAQVVRVLGFFQCHSQRGQVFHEAMKRFEMSR
EGLLQLFRRVGVTELEAISGTLPPASQRWHRILQALGVKGAKPSSASAQTQGQESLDAFADSLERELDAPS
PMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHG
DLAASSAAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
LW16-Tal3
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPAAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEAAHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCQPPYNLTPEQVVAIASHDGAKPALETVQRLLPVLCQ
PPYGLTPEQVVAIASKGGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASHDGAKQALETVQRLLLVLCQE
YGLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHNGGKQALETVQRLLPVLCQPPY
GLTPEQVVTIANNFGAKPALETVQRLLPVLCQPPYGLTPNQVVAIANNIGAKPALETVQRLLPVLCQPPYG
LTPEQVVAIAGNNGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHDGAKQALETVQRLLPVLCKPPHPL
TPNQVVAIASHDGAKQALETVQRLLPVLCKPPYGLTPDQVVAIASHDGAKQALETVQRLLPVLCKPPYGLT
PDQVVAIASHNGGKQALETVQRLLPVLCQPSYGLTRNQVVAIASHNGGKQALETVQRLLPVLCKEYGLTPE
QVVAIASHDGGTQALESIFAQLSSPDPALAALTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVP
EGTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWD
RMLQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQA
AEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQE
EIAWLKELLAH
LW16-Tal4
MEPIRSRTPSTARELQAGSQPDAVQPIADRLVSTPASSPLDGLPARRMVSRTSPPPSPARSPAFSAGSLSG
LLRQQIDPSLFAGSPFDSLPSFGAARAESAPGEGDEVQSGLRAVDDPQPSASAAITAAPPRTKAAARRRSA
QTSDALPAAHVDLGTFGYSQQQQEKIKPKVRSTLAQHHEALVGHGFTHAHIVELSKHPPALGTIAARYSEM
IAALPEATHEDIVGVGKQKSGARALEALLTVAEELRAPPLQLVTGQLLKIAKRGGVNAVEAVHASRNALTG
APLHLLPDQVVAIVSNHGSKLALGTVQRLLPVLCKPPYGLTRNQVVAIVNNNGGKQALETVHRLLPVLCQP
PYGLTPEQVVAIASHDGGRQALETVHRLLPVLRQPPYGLTLEQVVAIASNNGGKQALETVQRLLPVLCQPP
YGLTPDQVVTIASHDGGRQALETVQRLLPVLCQPPYGLTPNQVVAIASNHGGKQALETVQRLLPVLCQPLH
GLTPDQVVTIASHDGGRQALETVQRLLPVLCQPPYGLTPKQVVAIANYKGAKQALETVQRLLPLLCKPPYG
LTPDQVIAIVSNGGGKPALETVRRLLPVLCKHPYGLTPKQVVAIVSNHGGKPALETVRRLLPVLCKHPYGL
TPKQVVAIASYGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASHDGGRQALETVQRLLPVLRQPPYGLTP
DQVVAIASNNGGKQALETVQRLLPVLCQPPYSLIPDQVVAIASNIGAKQALETVQRLLPVLCQPPYGLTTD
QVIAIASNGGSKQALETVERLLPVLCQPPYGLTPDQVVAIASQDGGKQALESIFAQLSSPDPALAALTNDR
LVALACLGGRPALDAVKKGLPHAPALVTRTHNRVPEGTAHLVADHAQVVRVLGFFQCHSHPAQAFHEAMTR
FEMSREGLLQLFRRVGVTELEAISGTLPPASQRWHRILQALGVKGAKPSSASAQTPSQESVDAFADSLERE
LDAPSPIHEADRARASNRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLRLSSWGVKRQRTRIGGLPDPG
MPTDGELAASSAAFCEQDADPFAGAAEDFPVFDQEEIAWLMTLLPH
LW16-Tal5
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPAAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPSASAAITAAPPRTKAAARRRSAQ
TLDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSEMI
AALPEATHEDIVGVGKQWSGARALETLLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLTPDQVVAIVSNNGGKQALETVERLLPVLCQPPYGLTPNQVVAIASHDGAKPALETVQRLLPVLCQPP
26
YGLTPEQVVAIASNGGGKPALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLSLETVERLLPVLCQPPYG
LTPNQVVAIASHNGAKQALETVQRLLPVLCQPPYGLTPEQVVAIASHNGGKQALETVQRLLPVLCQPPYGL
TPEQVVAIVSNNGGKPALETVERLLPVLCQPPYGLTPNQVVAIASNIGAKPALETVQRLLPVLCQPPYGLT
PEQVVTIANNIGGKQALETVQRLLPVLRKPPYGLTPEQVVAIASNHGGKQALETVERLLPVLRKPPYGLTP
NQVVAIASNNGAKQALETVQRLLPVLCKPPHPLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTPE
QVVVIASNNGGKQALETVQRLLPVLCQPSYGLTPEQVVAIASNHGGKQALETVQRLLPVLCQPPYGLTPNQ
VVAIASHDGGKPALETVQRLLPVLCQPPYGLTPNQVVAIASHDGGTQALESIFAQLSSPDPALAALTNDRL
VALACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQF
GMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQASGRKGAKPSSASAQTPSQESVDAFADSLEREL
DAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGT
PTHGDLAASSGAFLEQDADPFAGAAEDFPAFDQEEIAWLKELLAH
LW16-Tal6
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVSQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALETLLMVAEELRAPPLQLVTGQLLKIAKGGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSHDGGKQSLETVQRLLPVLCQPPYGLTPNQVVAIASHNGGKQALETVQRLLPVLCQ
EYGLTPEQVVAIASHNGGKQALETVQRLLPVLCQEYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPY
GLTPEQVVAIASNHGGKLALETVERLLPVLCQPPYGLTPNQVVAIASNHGGKQALETVQPLLPVLCQPPYG
LTPEQVVAIASHGGAKQALKTVQRLLPVLCQDHGLTPEQVVAIANHDGAKQALETVQRLLPVLCQPPYGLT
PEQVVAIASKGGGKQALETVQRLLPVLCQPPYGLTPDQVVTIASNNGGKPALETVRRLLPVLCKPPYGLTP
KQVVAIASYGGKQALETVQRLLPVLCKPPYGLTPEQVVAIASNGGGKPALETVQRLLPVLCQEYGLTPNQV
VAIASHDGAKPALETVQRLLPVLCQEYGLTPNQVVAIASHDGGKPALETVQRLLPVLCQPPYGLTPEQVVA
IASHNGGTQALESIFAQLSSPDPALAALTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPEGTA
HLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFRRVGVTELEAISGTLPPASQRWDRMLQ
ASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAEVF
VPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSGAFLEQDADPFAGAAEDFPAFDQEEIAW
LKELLAH
LW16-Tal7
MDPIRSRTPSPARELLAGSQPDGVQPTADPRVSPPAGSPLDGLPARRTMSRTQLPPPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAEAATGELDEAQSALRAVDDPQPSASAAITAAPPRTKAAARRRSAQ
TLDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSEMI
AALPEATHEDIVGVGKQWSGARALEALLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALTGA
PLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCQPPYNLTPEQVVAIASHDGGKQALETVQRLLLVLCQEC
GLTPNQVVAIASHDGAKPALETVHRLLPVLCQPPYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQDHGL
TPGQVVAIADNIGGKQALETVQRLLPVLCKPPYGLTPEQVVTIANNIGGKPALETVQQLLPVLCQPPYGLT
PEQVVTIANNIGAKPALETVHRLLPVLRKPPYGLTPEQVVAIASHNGAKPALETVQRLLPVLCQPPYGLTP
NQVVAIASHDGAKQALETVQRLLPVLCKPPHPLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTPD
QVVVIASNNGGKQALETVQRLLPVLCKPPYGLTPEQVVAIASNNGGKPALETVQRLLPVLCQPPYDLTPDQ
VVAIANNIGAKPALETVQRLLPVLCQPPYGLTPEQVVAIASNNGGKQALETVQRLLPVLCQPPYGLTPYQV
VAIASHDGGTQALESIFAQLSSPDPALATLTNDRLVALACIGGRPALDAVKKGLPHAPELITRVHNRVPEG
TAHLVADHAQVVRVLGFFQCHSQRGQVFHEAMKRFEMSREGLLQLFRRVGVTELEAISGTLPPASQRWDRM
LQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAAE
VFVPEQRDAPLLLPLSSWGVKRRRTRIGGLPDTGMPTDGELAASSAAFLEQDADPFAGAAEDFPAFDQEEI
AWLRELLAH
LW16-Tal8
MDPIRSRTPSPARELQAGSQPDAVQPIADRLVSPPAGSPLDGLPARRTMSRTQLPSPPASVPAFSAGSFSD
LLRQVDSSLFDASFFDSMPAFGAHHAQAATGELDEVQSALRAADDPQPPVRVAVTAARPPRAKPAQRPRRA
AQTSDASPAADVDLSTFGYSQQQQEKIKPTVRSTVAQHHAALVGHGFTHAHIVELSKHPAALGTIAARYSE
MIAALPEATHEDIVGVGKQWSGARALETLLMVAEELRAPPLQLVTGQLLKIAKRGGVTAVEAVHASRNALT
GAPLHLTPDQVVAIVSNNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASHDGAKPALETVQRLLPVLCQ
PPYGLTPEQVVAIASNGGGKQALETVQRLLPVLCKEYGLTPEQVVAIASNNGGKLALETVERLLPVLCQPP
YGLTPKQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIACNGGGKQALETVQRLLPVLCQPPY
GLTPDQVVTIANNIGAKQALETVQRLLPVLCQPPYGLTPNQVVAIASHDGAKQALETVQRLLPVLCQEYGL
27
TPGQVVAIANNIGGKPALETVQRLLPVLCQPPYNLTPNQVVAIASNNGAKPALETVQRLLPVLCQPPYGLT
PNQVVAIASHDGAKQALETVQRLLPVLCKPPHPLTPNQVVAIASHDGAKQALETVQRLLPVLCQPPYGLTP
DQVVVIASNNGGKQALETVQRLLPVLCQPPYGLTPEQVVAIASNNGGKPALETVQRLLPVLCKPPYDLTPD
QVVAIANNIGAKPALETVQRLLPVLCQPPYGLTPEQVVAIASHNGGKQALETVQRLLPVLCQPPYGLTPYQ
VVAIASHDGGTQALESIFAQLSSPDPALATLTNDRLVALACIGGRPALDAVKKGLPHAPALITRVHNRVPE
GTAHLVADLAQVVRVLSFFQCHSHPAQAFDEAMRQFGMSRHGLLQLFHRVGVTELEARSGTLPPASQRWDR
MLQASGRKGAKPPSASAQTQGQESLDAFADSLERELDAPSPMHQAGQTLASSRKRSRSESSVNRSSAQQAA
EVFVPEQRDAPPLLPLSSWGVKRRRTRIGGLPDPGTPTHGDLAASSAAFLEQDADPFAGAAEDFPAFDQEE
IAWLKELLAH
Table S4
Table S4. NCED proteins of selected species and wheat.
>O49505.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED2,
chloroplastic; Short=AtNCED2; Flags: Precursor
MVSLLTMPMSGGIKTWPQAQIDLGFRPIKRQPKVIKCTVQIDVTELTKKRQLFTPRTTATPPQHNPLRLNI
FQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIPDCIDGVYIRNGAN
PMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFY
ARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLD
PVTKELHALSYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISG
KSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDE
SLRSVLSEIRINLRTRKTTRRSLLVNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSGFAKVDLCTGE
MKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYG
FHGTFVDSNELVDQL
>Q9LRR7.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED3,
chloroplastic; Short=AtNCED3; AltName: Full=Protein SALT TOLERANT 1;
Flags: Precursor
MASFTATAAVSGRWLGGNHTQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQSSNSPA
IVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVV
GKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKA
IGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRF
DFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFV
VVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVV
IGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLAL
AEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNAVSL
EVEATVKLPSRVPYGFHGTFIGADDLAKQVV
>Q9C6Z1.1 RecName: Full=Probable 9-cis-epoxycarotenoid dioxygenase
NCED5, chloroplastic; Short=AtNCED5; Flags: Precursor
MACSYILTPNPTKLNLSFAPSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEK
DTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGV
YLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGI
ARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAM
IAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFK
LSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADS
IFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFA
KVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVKLP
SRVPYGFHGTFVNSADMLNQA
>Q9LRM7.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED6,
chloroplastic; Short=AtNCED6; Flags: Precursor
MQHSLRSDLLPTKTSPRSHLLPQPKNANISRRILINPFKIPTLPDLTSPVPSPVKLKPTYPNLNLLQKLAA
TMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPP
28
LAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAG
IGLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTG
DLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPV
IYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSESGEP
TRVELSEIRLNMRTKESNRKVIVTGVNLEAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSE
FNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVS
ENQLKEQVF
>Q9M9F5.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED9,
chloroplastic; Short=AtNCED9; Flags: Precursor
MTIITIISGMYIYSLLSQDAHHSQYGQNTNLVLKKPIPKPQTAAFNQESTMASTTLLPSTSTQFLDRTFST
SSSSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQN
PKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVY
VRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIA
KLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMI
AHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRL
PEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMTPPDSI
FNEHDETLQSVLSEIRLNLKTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVD
LSTGEIRKYIYGEGKYGGEPLFLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPY
GFHGTFISKEDLSKQALC
>O65572.2 RecName: Full=Carotenoid 9,10(9',10')-cleavage dioxygenase 1;
AltName: Full=AtCCD1; AltName: Full=Neoxanthin cleavage enzyme NC1;
Short=AtNCED1
MAEKLSDGSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFL
PECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQEEFFGAAKFMKIGDL
KGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLLALQEADKPYVIKVLEDGDLQTLGIIDYDK
RLTHSFTAHPKVDPVTGEMFTFGYSHTPPYLTYRVISKDGIMHDPVPITISEPIMMHDFAITETYAIFMDL
PMHFRPKEMVKEKKMIYSFDPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLE
NPDLDMVSGKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDSIAK
VTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRETAEEDDGYLIFFVHDENTGKSCVT
VIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQLQEQTLI
>O49675.1 RecName: Full=Probable carotenoid cleavage dioxygenase 4,
chloroplastic; Short=AtCCD4; AltName: Full=AtNCED4; Flags: Precursor
MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTLHNRTNHTLVSS
PPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGAY
IRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASV
ARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSM
TAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMR
MNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMS
IEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKL
DVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEI
VAAVRLPRRVPYGFHGLFVKESDLNKL
>Q6YVJ0.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED1,
chloroplastic; Short=OsNCED1; Flags: Precursor
MQRICPAHCSVTHSLTMKSMRLSYIPPAASAAPQSPSYGRKKNASAAPPSAAASTTVLTSPLVTTTRTPKQ
TEQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAPAAAASAASLPMTFCNALEEVINTFIDPPALRP
AVDPRNVLTSNFVPVDELPPTPCPVVRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSP
ASSGDDPVLCSRYVQTYKYLVERDAGAPVLPNVFSGFHGVAGMARGAVVAARVLTGQMNPLEGVGLANTSL
AYFAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFRYGPVPPFVTY
FRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVVGGGSPVGSDPGKVPRLGVIPR
YATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPNVLSIEHALEHMELVHSCVEKVRINLRTGVVTR
TPLAAGNFDFPVINPAFLGRRNRYGYFGVGDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFV
ADDVEGNGNEDDGYLVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAELQSQHQ
29
>Q5MBR6.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED2,
chloroplastic; Short=OsNCED2; Flags: Precursor
MEVPIAAMTFAHPANVMTLASRQPKSKRSHISPATTAHRNLQTRLAHHHHATPASLPMAICNTVDKVINRF
IDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLAGGVYIRNGPNPQHRLPQRTHHLFDGDGM
LHSLLIPSASSTLLSEPVLCSRYVHTYKYLLERETGGPVLPNFFAGFHGVAGLARAVVMIARVLAGQINLN
KGFGLANTSITLFADCLYALCESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFR
YNVFQPFITYFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLDRT
MVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLGNMELMRARVDMVR
INLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIKGIRKLDFDLVGSGDCTVGRRDFGL
GCFAGEPFFVPDNIDGYGNEDSGYVVCYTHEEDTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVK
QAELLAQQ
>Q5MBR5.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED3,
chloroplastic; Short=OsNCED3; Flags: Precursor
MATITTPGYAHIQRQHGRCSTTAGRRGASNSVRFSARAVSSVPHAAAASSAPAFLPVPFVPGADAPSPSGK
SAIGVPKAPRKGEEGKRLNFFQRAAAMALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGETPPAR
ALPVSGRIPPFINGVYARNGANPHFDPVAGHHLFDGDGMVHAVRIRNGAAESYACRFTETARLRQERAMGR
PMFPKAIGELHGHSGIARLALFYARAACGLLDPSHGTGVANAGLIYFNGRLLAMSEDDLPYQVRVTADGDL
ETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKKPYLKYFYFAPDGTKSADVEIPLDQPTMIHDFA
ITENYVVVPDHQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMVWVDVPDCFCFHLWNAWEEAD
TDEVVVIGSCMTPADSIFNESDDRLESVLTEIRLNTRTGESTRRAILPPSSQVNLEVGMVNRNLLGRKTRY
AYLAVAEPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDAAAATPRGEDDGYILSFVHDERAGTS
ELLVVNAADMRLEATVQLPSRVPYGFHGTFITGDELTTQA
>Q69NX5.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED4,
chloroplastic; Short=OsNCED4; Flags: Precursor
MASSAPSAPGLAPVAKPPPPPSKVKVATATVPTNGKIKQGARPMRVSAPPVEPRRRMNPLQRLAAAAIDAV
EEGLVAGLLERGHALPRTADPAVQIAGNYAPVGERPPVRGLPVSGRLPACLDGVYVRNGANPLHAPRAGHH
LFDGDGMLHAVRLAGGRAESYACRFTETARLRQEREMGRPVFPKAIGELHGHSGVARLLLFGSRALCGVLD
ASRGIGVANAGLVYHDGRLLAMSEDDLPYHVRVTHDGDLETVGRYDFHGQLDADGTMIAHPKLDPVTGELF
ALSYNVVSKPYLKYFYFTADGRKSRDVDIPVGAPTMIHDFAVTENYAVVPDQQIVFKLQEMVRGGSPVVYD
REKASRFGVLPKRAADASELRWVEVPGCFCFHLWNAWEDDATGEIVVIGSCMTPPDAVFNEPSQSPEEESF
RSVLSEIRLDPRTGVSRRRDVLRDAAEQVNLEAGMVNRQLLGRKTRYAYLAIAEPWPRVSGFAKVDLESGT
AEKFIYGEGRYGGEPCFVPRAGAAAEDDGHVLCFVHDEERGTSELVVVDAGSEAMEEVAAVKLPGRVPYGL
HGTFIGANELQRQA
>Q5MBR3.1 RecName: Full=9-cis-epoxycarotenoid dioxygenase NCED5,
chloroplastic; Short=OsNCED5; Flags: Precursor
MPTTFTPNSPASSCSIHHRASPSRGARNSVRFTRPRAAAAATNSVLSAPSSVPPAYVPPPPPPPTKMFPEA
GDAAAAKAAARRCGKKKDGLNFFQRAAAVALDAFEEGFITNVLERPHALPRTADPAVQIAGNFAPVGEQPP
VRSLPVSGRIPPFINGVYARNGANPHFEPTAGHHLFDGDGMVHAVRIRNGAAESYACRFTETARLGQERAL
GRAVFPKAIGELHGHSGIARLALFYARGLCGLVDPSHGTGVANAGLVYFNGRLLAMSEDDLPYQVRVTADG
DLETVGRYDFDGQLGCAMIAHPKLDPVSGELFALSYDVIKKPYLKYFYFDADGTKSPDVEIELEQPTMIHD
FAITENFVVVPDHQVVFKLGEMFRGGSPVVLDREKTSRFGVLPKHATSSLEMVWVDVPDCFCFHLWNAWEE
AESGEVVVVGSCMTPADSIFNESDEHLESVLTEIRLNTRTGESTRRAVLPPAAQVNLEVGMVNRAMLGRKT
RYAYLAVAEPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMGGAGAAASPARGEDDGYILSFVRDE
AAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFINAGELATQA
>O24592.2 RecName: Full=9-cis-epoxycarotenoid dioxygenase 1,
chloroplastic; AltName: Full=Protein VIVIPAROUS14; Short=VP-14;
Short=VP14; Flags: Precursor
MQGLAPPTSVSIHRHLPARSRARASNSVRFSPRAVSSVPPAECLQAPFHKPVADLPAPSRKPAAIAVPGHA
AAPRKAEGGKKQLNLFQRAAAAALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGERPPVHELPVS
GRIPPFIDGVYARNGANPCFDPVAGHHLFDGDGMVHALRIRNGAAESYACRFTETARLRQERAIGRPVFPK
AIGELHGHSGIARLALFYARAACGLVDPSAGTGVANAGLVYFNGRLLAMSEDDLPYHVRVADDGDLETVGR
YDFDGQLGCAMIAHPKLDPATGELHALSYDVIKRPYLKYFYFRPDGTKSDDVEIPLEQPTMIHDFAITENL
VVVPDHQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMAWVDVPDCFCFHLWNAWEDEATGEVV
30
VIGSCMTPADSIFNESDERLESVLTEIRLDARTGRSTRRAVLPPSQQVNLEVGMVNRNLLGRETRYAYLAV
AEPWPKVSGFAKVDLSTGELTKFEYGEGRFGGEPCFVPMDPAAAHPRGEDDGYVLTFVHDERAGTSELLVV
NAADMRLEATVQLPSRVPFGFHGTFITGQELEAQAA
>TaNCED 5DL (BAS30382)
MQTLTASTSVSSIQRHRPRPAGRSSSVSFTARAVSSVPRAPSGAARAPAPSQFVRGADAKPLIAVPKAPAV
ERQEKKLNFFQRAAATALDAFEEGFVANVLERPHGLSRTVDPAVQIAGNFAPVGETPPVHALPVTGRIPPF
INGVYARNGANPRFDPVAGHHLFDGDGMVHALRIRNGVAETYASRFTETERLQQERALGRPMFPKAIGELH
GHSGIARLALFYARAACGLIDPSRGTGVANAGLVYFNGHLLAMSEDDIPYHVRVTDDGDLQTVGRYDFDGQ
LECPMIAHPKLDPATGELHALSYDVIKKPYLKYFYFAADGTKSADVEIPLDQPTMIHDFAITENYVVVPDH
QVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKCAADASEMVWVDVPDCFCFHLWNAWEEEETEEVVVIGSCM
TPADSIFNESDECLESVLTEIRLNTRTGESTRRPILALSEQVNLEVGMVNSNLLGRKTRYAYLAVAEPWPK
VSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDPAASRGEDGYILTFVHDEAAGTSELLVVNAADMRLEA
TIQLPSRVPYGFHGTFVTGKELESQA
>TaNCED 5AL (AOE46767)
MQTLTASTSVSSIQRHTPRRTGRSGSLSFSARAVSSPPRAPAPSRFVRGADAAPAKPLIAVPKAPAVERQE
KKLNFFQRAAATALDAFEEGFVANVLERPHGLSRTVDPAVQIAGNFAPVGETPPVQALPVTGRIPPFINGV
YARNGANPHFDPVAGHHLFDGDGMVHALRIRNGVAETYASRFTETERLQQERALGRPMFPKAIGELHGHSG
IARLALFYARAACGLIDPSRGTGVANAGLVYFNGHLLAMSEDDIPYHVRVTDDGDLQTVGRYDFDGQLECP
MIAHPKLDPATGELHALSYDVIKKPYLKYFYFKADGTKSADVEIPLDQPTMIHDFAITENYVVVPDHQVVF
KLQEMLRGGSPVVLDKEKTSRFGVLPKCAADASEMVWVDVPDCFCFHLWNAWEEEESDEVVVIGSCMTPAD
SIFNESDECLESVLTEIRLNTRTGESTRRPILALSEQVNLEVGMVNSNLLGRKTRYAYLAVAEPWPKVSGF
AKVDLATGELTKFEYGEGRFGGEPCFVPMDPAASRGEDDGYILTFVHDEAAGTSELLVVNAADMRLEATIQ
LPSRVPYGFHGTFVTGKELESQA
>TaNCED 5BL (referred from TGAVv1 genome)
MQTLTASTSVSSIQRHRPRPAGRSSFSARAVSSAPRAPSGAARVPAPSRFVRGADAAPAKPLIAVPKAPAV
ERQEKKLNFFQRAAATALDAFEEGFVANVLERPHGLSRTVDPAVQIAGNFAPVGETPPVHALPVTGRIPPF
INGVYARNGANPHFDPVAGHHLFDGDGMVHALRIRNGVAETYASRFTETERLQQERALGRPMFPKAIGELH
GHSGIARLALFYARAACGLIDPSRGTGVANAGLVYFNGHLLAMSEDDIPYHVRVTDDGDLQTVGRYDFDGQ
LECPMIAHPKLDPATGELHALSYDVIKKPYLKYFYFKADGTKSADVEIPLDQPTMIHDFAITENYVVVPDH
QVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKCAADSSEMVWVDVPDCFCFHLWNAWEEEETDEVVVIGSCM
TPADSIFNESDECLESVLTEIRLNTRTGESTRRPILALSEQVNLEVGMVNSNLLGRKTRYAYLAVAEPWPK
VSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMDPATSRGEDDGYILTFVHDEAAGTSELLVVNAADMRLE
ATIQLPSRVPYGFHGTFVTGKELESQA
>TaNCED 5DS (referred from TGAVv1 genome)
MQTLSAQPLASSSASSSIQHHHGRRRGASSVRFAPCAAAAAADSVLIAASTSTARTPAYVSSPSTRKVPGY
EQSGPPAIASPHKQGSSSGKDGQSLNFFQRAAAAALDAFEEGFIHNVLERPHALPRTADPAVQIAGNFAPV
GEQAPVRALPVSGRIPPFINGVYARNGANPCFEPTAGHHLFDGDGMVHAIRIRNGAAESYTCRFTETARLS
QERAVGKPVFPKTIGELHGHSGIARLALFYARGACGLVDPSHGTGVANAGLVYFNGRLLAMSEDDLPYQVR
VTADGDLETVGRYDFDGQLDCAMIAHPKLDPVSGELFALSYDVIKKPYLKYFYFHADGTKSADVEIELDQP
TMIHDFAITENFVVVPDHQMVFKLAEMFRGGSPVMLDKEKTSRFGVLPKYAKHSSEMMWVDVPDCFCFHLW
NSWEEPETDEVVVIGSCMTPADSIFNDTDDHLESVLTEIRLNTRTGESTRRAILPLESQVNLEVGMVNRNM
LGRKTRYAYLAVAEPWPKVSGFAKVDLVTGELTKFEYGEGRFGGEPCFVPMDGEHARPGAEDDGYVLSFVR
DEAAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFIGAADLDAQH
>TaNCED 5AS (referred from TGAVv1 genome)
MQTLSAQPLASSSSSIQRQNGRRRGTSSVRFAPRAAAAAADSVLIAASTSTARPPAYLPSPSTRKVPGYEQ
SAPPAIASPQKQGSSKDGQSKGGLNFFQRAAAAALDAFEEGFIHNVLERPHALPRTADPAVQIAGNFAPVG
EQAPVRALPVSGRIPPFINGVYARNGANPCFEPTAGHHLFDGDGMVHAIRIRNGAAESYACRYTETARLSQ
ERAVGRPIFPKTIGELHGHSGIARLALFYARGACGLVDPSHGTGVANAGLVYFNGRLLAMSEDDLPYQVRV
TAGGDLETVGRYDFDGQLDCAMIAHPKLDPVSGELFALSYDVIKKPYLKYFYFHADGTKSADVEIELDQPT
MIHDFAITENFVVVPDHQMVFKLAEMFRGGSPVMLDKEKTSRFGVLPKYAKDSSEMMWVDVPDCFCFHLWN
SWEEPETDEVVVIGSCMTPADSIFNDTDDHLESVLTEIRLNTRTGESTRRTILPLESQVNLEVGMVNRNML
31
GRKTRYAYLAVAEPWPKVSGFAKVDLVTGELTKFEYGEGRFGGEPCFVPMDGEHARPGAEDDGYVLSFVRD
EAAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFIGAADLDAQH
>TaNCED 5BS (referred from TGAVv1 genome)
MQTLSAQPLASSSASSSIQHHHGRRRGASSVRFSPRAAAAAADSVLIAASTSTARTPAYVSSPSTRKVPGY
EQSALPAIASPQKQSGSGKDGQSLNFFQRAAAAALDAFEEGFTHNVLERPHALPRTADPAVQIAGNFAPVG
EQAPVRALPVSGRIPPFINGVYARNGANPCFEPTAGHHLFDGDGMVHAIRIRNGAAESYACRFTETARLSQ
ERAVGRPVFPKTIGELHGHSGIARLALFYARGACGLVDPSHGTGVANAGLVYFNGRLLAMSEDDLPYQVRV
TAAGDLETVGRYDFDGQLDCAMIAHPKLDPVSGELFALSYDVIKKPYLKYFYFHADGTKSADVEIELDQPT
MIHDFAITENFVVVPDHQMVFKLAEMFRGGSPVMLDKEKTSRFGVLPKYAKESSEMMWVDVPDCFCFHLWN
SWEEPETDEVVVIGSCMTPADSIFNDTDDHLESVLTEIRLNTRTGESTRRAILPLESQVNLEVGMVNRNML
GRKTRYAYLAVAEPWPKVSGFAKVDLVTGELTKFEYGEGRFGGEPCFVPMDGEHARPGAEDDGYVLSFVRD
EAAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFIGAADLDAQH
>TaNCED 2BL (referred from TGAVv1 genome)
MASSITAPAAAPGSVVRPPAKVRPRPPPSRFNPNDPRKNADGSAVVTWKGPMRAAPARKKWPNPFQRMVAA
ALNAVEDRLVAGILERKHPLPRTADPAVQIAGNYAPVGELPPAGPEDLPVVSGRVPACLQGVYVRNGANPL
HAPRAGHHLFDGDGMVHAVRLGGGRAESYACRFTKTARLRQEREIGRPIFPKAIGELHGHSGVARLALFGA
RSLCGVVDASQGIGVANAGLVYHDGRLLAMSEDDLPYHVRVTPDGDLETVGRYDFNGQLDGAMIAHPKLDP
ATGELFALSYNVVSKPYLKYFYFTADGHKSHDVDIPVDEPTMIHDFAVTENYAVVPDQQIVFKLREMVLGG
SPVVYDKSKTARFGVVPKRAANASELRWVEVPDCFCFHLWNAWEDEATGEIVVIGSCMTPPDAVFNESPDQ
DQSFRSVLSEIRLDPRTGTSRRRAVLREADQVNLEAGMVNRQLVGRKTRYAYLSIAEPWPRVSGFAKVDLE
SGTVEKFTC
>TaNCED 2DL (SPT17866)
MASSITAPAAAPGSVVRPPAKVAPNDPRKNADGSAVVTWKGPMRAAPARKKWPNPFQRMVAAALDAVEDRL
VAGILERKHPLPRTVDPAVQIAGNYAPVGELPPAGPEDLPVVSGRVPACLEGVYVRNGANPLHAPRAGHHL
FDGDGMLHAVRLGGGRAESYACRFTETARLRQEREIGRPIFPKAIGELHGHSGVARLALFGARSLCGVVDA
SQGIGVANAGLVYHDGRLLAMSEDDLPYHVRVTPDGDLETVGRYDFNGQLDGAMIAHPKLDPATGELFALS
YNVVSKPYLKYLYFTADGHKSHDVDIPVDEPPMIHDFAVTENYAVVPDQQIVFKLQEMVLGGSPVVYDKNK
TARFGVLPKRAANALELRWVEVPDCFCFHLWNAWGGEATGEIVVIGSCMTPPDAVFNESPDQEQSFRSVLS
EIRLDPRTGTSRRRAVLREADQVNLEAGMVNRQLLGRKTRYAYLAIAEPWPRVSGFAKVDLESGTAEKFTY
GEGRYGGEPCFVPHADGSGAEDDGYVLCFVHDESRGSADGTSSELLVVNARDLRSEATVELPGRVPYGFHG
TFITATELQRQAQL
>TaNCED 2AL (referred from TGAVv1 genome)
MASSITAPAAAPGSVVRPPAKVRPRPPSSRFNPNDPRRNADGSAVVTWKGPMRAAPARKKWPNPFQRMVAA
ALDAVEDRLVVGILERKHPLPRTADPAVQISGNYAPVGELPPAGPEDLPVVSGRVPACLEGVYVRNGANPL
HAPRAGHHLFDGDGMLHAVRLGGGRAESYACRFTETARLRQEREIGRPIFPKAIGELHGHSGVARLALFGA
RSICGVVDASQGIGVANAGLVYHDGRLLAMSEDDLPYHVRVTPDGDLETVGRYDFNGQLDGAMIAHPKLDP
ATGELFALSYNVVSKPYLKYFYFTADGHKSHDVDIPVDEPTMIHDFAVSENYAIVPDQQIVFKLQEMVLGG
SPVVYDKNKTARFGVLPKRAANASDLRWVEVPGCFCFHLWNAWEDEATGEIVVIGSCMTPPDAVFNESPDQ
EQSFRSVLSEIRLDPRTGTSRRRAVLREADQVNLEAGMVNRQLLGRKTRYAYLAISEPWPRVSGFAKVDLE
SGTVEKFTYGEGRYGGEPCFVPHADGSGAEDDGYVLCFVHDESRGNADGTSSELLVVNARDLRSEATVKLP
GRVPRISRHVHHRHGAAATSPAIAS
32
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