The Effect of Allium Sativum, Camellia
Sinensis and Penicillin on the
Growth of Escherichia Coli Bacteria
Stephanie Close – Sterling Heights High School
Claire St. Louis – Warren Mott High School
Why
?• Antibiotic resistance• Household remedies
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Bac
kgro
und
• Escherichia Coli– What is E.Coli bacteria?– Reproduction process
http://fineartamerica.com/art/photographs/petri+dish+culture+e.coli/all
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Bac
kgro
und
• Penicillin– What is penicillin?– How it kills bacteria
http://en.wikipedia.org/wiki/Penicillin
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und
• Allium Sativum– What is Allium Sativum?– Antibacterial Agents
http://carolcassara.com/2013/11/how-to-roast-garlic.html
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Bac
kgro
und
• Camellia Sinensis– What is Camellia Sinensis?– Antibacterial Agents
http://www.wisegeek.com/what-is-camellia.htm#
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Bac
kgro
und
• Zone of Inhibition
http://www.antimicrobialtestlaboratories.com/Zone_of_Inhibition_Test_for_Antimicrobial_Activity.htm
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Prob
lem
• To determine the effect of garlic powder, green tea, and penicillin on the growth of E.coli bacteria
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Hyp
othe
sis
• Penicillin (g) will create the largest zone of inhibition (mm) while green tea (g) will create the smallest zone of inhibition (mm)
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Mat
eria
ls E.Coli
Garlic PowderGreen Tea
Petri Dishes Coffee FiltersPenicillin
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Proc
edur
es
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Dat
a
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Treatment Overall Average Standard Deviation Penicillin 1.1 0.3
Garlic Powder 1.1 0.2Green Tea 1.6 0.2
Obs
erva
tions
• Large bacteria colonies• Green tea stain• Undissolved garlic powder
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Anal
ysis
Zone of Inhibition (mm)
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2 S
ampl
e t-T
est
• Assumptions- 2 SRS from two distinct populations- Populations are independent - Normal Distribution- Population means and population
standard deviations are not known
2
22
1
21
21
ns
ns
xx
t =
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2
22
1
21
21
ns
ns
xx
t =
Con
clus
ion
• Ho: μ1 = μ2
• Ha: μ1 ≠ μ2
• Garlic Powder vs. Penicillin- Failed to reject Ho
• Green Tea vs. Penicillin- Reject Ho
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Err
ors
• Treatment Solutions• Placement of treatment
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Con
clus
ion
• Hypothesis: Rejected• Green tea created the largest zone of
inhibition
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Furt
her
Res
earc
h• Various factors• DOE test
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Rec
ap• Problem• Hypothesis• Conclusion
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Res
ourc
es• http://www.elmhurst.edu/~chm/vchembook/652penicillin.html• http://allicincenter.com/pdf/Allicin.pdf• http://www.webmd.com/vitamins-and-supplements/black-tea-uses-
and-risks.com• http://www.westminster.edu/acad/sim/pdf/
santibioticinhibitionofbacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com• http://www.nlm.nih.gov/medlineplus/druginfo/meds/a682098.html• http://www.microbelibrary.org/component/resource/laboratory-test/
3085-preparing-spread-plates-protocols.com• http://www.studymode.com/essays/The-Effects-Of-Black-Tea-On-
1164976.html• http://www.all-sciencefairprojects.com/science_fair_pr ojects.html• http://www.ncbi.nlm.nih.gov/pubmed/23847105.com• http://www.ncbi.nlm.nih.gov/pubmed/15260343.com• http://www.drlwilson.com/articles/antibiotics.htm• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://community.middlebury.edu/~sontum/chemistry/students/
atteridge/penicillin/penicillin.html• http://www.nutrimart.com/bulk/description/greentea.htm• http://examine.com/supplements/Green+Tea+Catechins/• http://www.ncbi.nlm.nih.gov/pubmed/21827739• http://healthyeating.sfgate.com/egcg-green-tea-8453.html• http://www.drhoffman.com/page.cfm/118• http://www.amazon.com/Foods-Green-Extract-400mg-Vcaps/dp/
B001DNV5CA• http://royalmajestea.com/basics.htm• http://community.middlebury.edu/~sontum/chemistry/students/
atteridge/penicillin/penicillin.html• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://www.teavivre.com/info/green-tea-history/• http://www.westminster.edu/acad/sim/pdf/santibioticinhibition/
bacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com
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Ackn
owle
dgm
ents
• Mr. Estapa • Mrs. Gravel• Bradley Rabquer
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