Chemical and Biological Studies on Allium sativum L. (1952 ...
The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the
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Transcript of The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the
The Effect of Allium Sativum, Camellia
Sinensis and Penicillin on the
Growth of Escherichia Coli Bacteria
Stephanie Close – Sterling Heights High School
Claire St. Louis – Warren Mott High School
Why
?• Antibiotic resistance• Household remedies
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Bac
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• Escherichia Coli– What is E.Coli bacteria?– Reproduction process
http://fineartamerica.com/art/photographs/petri+dish+culture+e.coli/all
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• Penicillin– What is penicillin?– How it kills bacteria
http://en.wikipedia.org/wiki/Penicillin
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• Allium Sativum– What is Allium Sativum?– Antibacterial Agents
http://carolcassara.com/2013/11/how-to-roast-garlic.html
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• Camellia Sinensis– What is Camellia Sinensis?– Antibacterial Agents
http://www.wisegeek.com/what-is-camellia.htm#
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• Zone of Inhibition
http://www.antimicrobialtestlaboratories.com/Zone_of_Inhibition_Test_for_Antimicrobial_Activity.htm
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Prob
lem
• To determine the effect of garlic powder, green tea, and penicillin on the growth of E.coli bacteria
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Hyp
othe
sis
• Penicillin (g) will create the largest zone of inhibition (mm) while green tea (g) will create the smallest zone of inhibition (mm)
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Mat
eria
ls E.Coli
Garlic PowderGreen Tea
Petri Dishes Coffee FiltersPenicillin
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Proc
edur
es
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Dat
a
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Treatment Overall Average Standard Deviation Penicillin 1.1 0.3
Garlic Powder 1.1 0.2Green Tea 1.6 0.2
Obs
erva
tions
• Large bacteria colonies• Green tea stain• Undissolved garlic powder
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Anal
ysis
Zone of Inhibition (mm)
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2 S
ampl
e t-T
est
• Assumptions- 2 SRS from two distinct populations- Populations are independent - Normal Distribution- Population means and population
standard deviations are not known
2
22
1
21
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ns
ns
xx
t =
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2
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ns
ns
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t =
Con
clus
ion
• Ho: μ1 = μ2
• Ha: μ1 ≠ μ2
• Garlic Powder vs. Penicillin- Failed to reject Ho
• Green Tea vs. Penicillin- Reject Ho
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Err
ors
• Treatment Solutions• Placement of treatment
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Con
clus
ion
• Hypothesis: Rejected• Green tea created the largest zone of
inhibition
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Furt
her
Res
earc
h• Various factors• DOE test
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Rec
ap• Problem• Hypothesis• Conclusion
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Res
ourc
es• http://www.elmhurst.edu/~chm/vchembook/652penicillin.html• http://allicincenter.com/pdf/Allicin.pdf• http://www.webmd.com/vitamins-and-supplements/black-tea-uses-
and-risks.com• http://www.westminster.edu/acad/sim/pdf/
santibioticinhibitionofbacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com• http://www.nlm.nih.gov/medlineplus/druginfo/meds/a682098.html• http://www.microbelibrary.org/component/resource/laboratory-test/
3085-preparing-spread-plates-protocols.com• http://www.studymode.com/essays/The-Effects-Of-Black-Tea-On-
1164976.html• http://www.all-sciencefairprojects.com/science_fair_pr ojects.html• http://www.ncbi.nlm.nih.gov/pubmed/23847105.com• http://www.ncbi.nlm.nih.gov/pubmed/15260343.com• http://www.drlwilson.com/articles/antibiotics.htm• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://community.middlebury.edu/~sontum/chemistry/students/
atteridge/penicillin/penicillin.html• http://www.nutrimart.com/bulk/description/greentea.htm• http://examine.com/supplements/Green+Tea+Catechins/• http://www.ncbi.nlm.nih.gov/pubmed/21827739• http://healthyeating.sfgate.com/egcg-green-tea-8453.html• http://www.drhoffman.com/page.cfm/118• http://www.amazon.com/Foods-Green-Extract-400mg-Vcaps/dp/
B001DNV5CA• http://royalmajestea.com/basics.htm• http://community.middlebury.edu/~sontum/chemistry/students/
atteridge/penicillin/penicillin.html• http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html• http://www.teavivre.com/info/green-tea-history/• http://www.westminster.edu/acad/sim/pdf/santibioticinhibition/
bacteria.pdf• http://mmbr.asm.org/content/65/2/232.full• http://pathmicro.med.sc.edu/fox/antibiotics1.htm• http://www.antibiotics-info.org/antibiotic-drugs.html• http://onlinelibrary.wiley.com/doi/10.1038.com
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Ackn
owle
dgm
ents
• Mr. Estapa • Mrs. Gravel• Bradley Rabquer
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