Tg(hsp70:mcherry; CMV:naos3’UTR:GFP)24

hrs

8

hrs

16 h

rs

Express EGFP in germ cell

Tg(dkk1b-2Ki1)

Express EGFP in pharyngeal arch and otic vesicle.

TgIBStrap1D

ay 2

D

ay 1

Expressed in lateral line

Tg(gnrh3-GFP-LacZ)

Expression and migration of GFP- and LacZ-expressing

cells. A, B, F, G, ventral view; C-E, dorsal view. A, C, F, G,

GFP cells; D, E, LacZ-expressing cells. A, GFP expression at

the olfactory placode (OP). B-D, GFP or LacZ cells begin to

migrate into telencephalon (Tel) at 3-8 d (days). E, lacZ

cells and axons were detected in brain at 16dpf. F-G, At 1

month (m), fluorescent axonal extensions are detected at

OB, Tel and POA. Red arrows indicate cell bodies, yellow

arrows indicate axons. POA, preoptic area.

2k1k

1kE1

2k:GFP; 1d 2k:GFP; 2d 2k:GFP; 3d

1k:GFP; 1d 1k:GFP; 2d 1k:GFP; 3d

1kE1:GFP; 1d 1kE1:GFP; 2d 1kE1:GFP; 3d

C D E

F G H

I J K

4k

4k:GFP; 1d 4k:GFP; 2d 4k:GFP; 3d

L M N

Tg-dkk1b-1k ,1kE1, 2k, 4k

Expressed in pharyngeal arches, otic vesicle, whole body(4k)

Tg(zpopdc1:GFP)

Tg(J11:EGFP)