International Symposium on Synthetic
Biology in Photosynthesis Research
Symposium Handbook
Aug 08-10, 2018
Shanghai, China
Supported by
Center of Excellence for Molecular Plant Science, CAS
Shanghai Institute of Plant Physiology and Ecology, SIBS, CAS
The ARC Centre of Excellence for Translational Photosynthesis, ANU
State Key Laboratory of Hybrid Rice
Chinese Society for Plant Biology
Sponsored by
The ARC Centre of Excellence for Translational Photosynthesis, ANU
European Union Europe Aid SEW‐REAP project
State Key Laboratory of Hybrid Rice
Chinese Society for Plant Biology
Organized by
Center of Excellence for Molecular Plant Science, CAS
Shanghai Institute of Plant Physiology and Ecology, SIBS, CAS
Chinese Society for Plant Biology
Exhibitions
Beijing Ecotek Technology Co., Ltd.
Biogle Gene Technology (Jiangsu) Co., Ltd.
PhenoTrait (Beijing) Scientific Technology Co. Ltd.
Zealquest Scientific Technology Co., Ltd.
International Symposium on Synthetic
Biology in Photosynthesis Research
Symposium Handbook
Aug 08-10, 2018
Shanghai, China
Sponsored by
The ARC Centre of Excellence for Translational Photosynthesis, ANU
European Union Europe Aid SEW‐REAP project
State Key Laboratory of Hybrid Rice
Chinese Society for Plant Biology
Contents
Welcome message 1
Symposium Notice 2
Warm Reminder 4
Symposium Schedule 8
Scientific Program 9
Abstracts of Invited Talks 13
Session 1 Photosynthesis systems
Improving Photosynthetic Efficiency for Improved Crop Yield (Donald R. Ort) 14
Respiratory Metabolism during Photosynthesis (Kevin L. Griffin) 15
Mesophyll CO2 Conductance in C3 and C4 plants (Asaph B. Cousins) 16
Variations of C4 Photosynthetic Pathways and Their Physiological Significance
(Yu Wang) 17
Re-engineering the Carbon Shuttle Pathway in Setaria viridis (Thomas P.
Brutnell) 18
Session 2 Engineering photosynthetic systems
Engineering Photorespiration: A Synthetic Biology Approach to Improving Crop
Productivity (Paul F. South, Amanda P. Cavanagh, Helen W. Liu, Donald R.
Ort)
19
Modeling C4 Photosynthesis and Moonlighting in CAM (Andrea Bräutigam) 20
How to Build a Carboxysome: Progress and Future Challenges to Constructing
Functional Carboxysomes in the Chloroplast (Ben Long) 21
Electron Transport in C4 Plants (Maria Ermakova, Furbank Robert, Susanne von
Caemmerer) 22
Lessons from Synthetic Engineering of Carbon Fixation (Ron Milo) 23
Reconstitution of the Cyanobacterial Carbon Concentrating Mechanism in Rice
Chloroplasts to Improve Yield (Page M T, Qu M, Perveen S, Hanson M R, Zhu
X, Lin M T, Orr D J, Carmo-Silva E, Martin Parry)
24
Novel Insights into the Regulation of C4 Photosynthesis (Susanne von
Caemmerer, Jasper Pengelly, Florence Danila, Maria Ermakova, Hannah L
Osborn, Hugo Alonso-Cantabrana1, Rosemary White, Robert T Furbank)
25
Session 3 Tools sets for synthetic biology in photosynthesis
Probing Cellular Redox Metabolism Using Genetically Encoded Fluorescent
Sensors (Yi Yang) 26
Flux Analysis of Arabidopsis Primary Metabolism (Fangfang Ma, Doug K.
Allen) 27
Three-dimensional Modeling of Rice Photosynthesis (Yi Xiao) 28
Understanding and Manipulating Metabolic Fluxes in Cyanobacteria
(Chen Yang) 29
Metabolite Flux Analysis in Maize (Stephanie Arrivault) 30
Abstracts of Posters 31
Session 1 Photosynthesis systems
No.01: Effect of different metals (Lead and Zinc) on chlorophyll fluorescence
in black gram and its partial recovery by brassinosteroid (Alok Srivastav, V.P.
Singh)
32
No.02: Differential responses of mesophyll conductance to temperature at three
different O2 concentrations in rice plants (Guanjun Huang, Yong Li) 33
No.03: Cyclic electron flow can protect PSII against photoinhibition in rice
following heat stress (Jemaa Essemine, Mingnan Qu, Genyun Chen, Xinguang
Zhu)
34
No.04: Seasonal variations of sun-induced chlorophyll fluorescence from leaf
to canopy level and its relations with plant traits for paddy-rice (Ji Li, Yongguang
Zhang, Qian Zhang, Zhaohui Li, Jing Li, Jingming Chen)
35
No.05: Sensitive response of chloroplast size to leaf nitrogen content at the
tillering stage resulted in the decreased photosynthetic nitrogen use efficiency
(PNUE) in rice (Oryza sativa L.) plant (Limin Gao)
36
No.06: Synthesis of structural carboxysomes in tobacco chloroplasts (WeiYih
Hee) 37
No.07: Response of photosynthetic efficiency and NPQ based photoprotection
of rice plants grown under different LED light wavelength (red, blue and white)
(Saber Hamdani, Naveed Khan, Shahnaz Perveen, Mingnan Qu, Jianjun Jiang,
Govindjee, Xinguang Zhu)
38
No.08: The photosynthetic responses of Panicum antidotale under salinity,
drought and combination of both stresses (Tabassum Hussain, Xiaojing Liu) 39
No.09: Cryo-EM structure of maize PSI-LHCI-LHCII supercomplex (Xiaowei
Pan, Jun Ma, Xiaodong Su, Wenrui Chang, Zhenfeng Liu, Xinzheng Zhang, Mei
Li)
40
No.10: Low level of HCO3- content involved in drought response in transgenic
rice with overexpression C4-PEPC (Jinfei Zhang, Xia Li, Yinfeng Xie) 41
No.11: Concerted decreases in leaf photosynthesis and hydraulic conductance
under K deficiency: prominent roles of mesophyll conductance to CO2 and
outside-xylem hydraulic conductance (Zhifeng Lu, Shiwei Guo)
42
Session 2 Engineering photosynthetic systems
No.12: Engineering photosynthesis by altering cell division patterns in the
leaf (Andrew Fleming) 43
No.13: A dynamic model of primary metabolism in C3 leaf (Honglong Zhao,
Xinguang Zhu) 44
No.14: The evolution of PPT1 and its bidirectional role in C4 species (Ming-
Ju Amy Lyu, Jianjun Jiang, Yaling Wang, Xinyu Liu, Xinguang Zhu) 45
No.15: CO2 control system based on an optimized regulation model (Pingping
Xin, Jin Hu, Haihui Zhang) 46
No.16: Systematic optimization of whole plant carbon nitrogen interaction
(WACNI) to support crop design for greater yield (Tiangen Chang, Xinguang
Zhu)
47
No.17: Identification and expression of the key genes involved in C4
photosynthetic pathway in bread wheat (Yingang Hu, Daoura Gaoh Goudia
Bachir, Yang Yang, Liang Chen)
48
No.18: Plasmodesmatal flux in C3 and C4 monocots: the metabolite pathway
between mesophyll and bundle sheath cells (Florence Danila, Susanne von
Caemmerer)
49
Poster Location and Hanging 50
The Floor Plan for Exhibitions 50
A Brief Introduction to Shanghai Institute of Plant Physiology
and Ecology (SIPPE), SIBS, CAS 51
A Brief Introduction to Laboratory of Photosynthesis and
Environmental Biology, SIPPE, SIBS, CAS 53
Participants' Information 55
Notes of Symposium 64
1
Welcome message
Photosynthesis provides the basis for food, energy, fiber and healthy
environment for humanity; it is also a critical component of the global
ecosystem. Understanding how photosystem works and how to improve its
efficiency are a major focus of the contemporary photosynthesis biology
research. The International Symposium on Synthetic Biology in
Photosynthesis Research aims to bring together scientists working in the
various fields closely related to synthetic biology and photosynthesis research
areas to discuss the recent progress and brainstorm future opportunities. The
meeting dates shall hold from August 8 through August 10.
The symposium will be organized around three topic areas, i.e.
understanding photosynthesis systems, engineering photosynthetic systems,
and new technologies/tools for synthetic biology. Plenty of time will be
allocated for the discussion sessions to ensure close interaction between the
speakers and audiences. At the end, we will organize a discussion session to
brainstorm unexplored opportunities on photosynthesis synthetic biology,
methods and tools needed to realize these opportunities, and maximize the
impact of photosynthetic synthetic biology on agriculture.
We look forward to having your participation at the meeting in the Aug of
2018!
Best wishes,
Xinguang Zhu
Susanne von Caemmerer
Chair of the Symposium
March 28, 2018
2
International Symposium on Synthetic
Biology in Photosynthesis Research (Symposium Notice)
Photosynthesis provides the basis for food, energy, fiber and healthy
environment for humanity; it is also a critical component of the global
ecosystem. Understanding how photosystem works and how to improve its
efficiency are a major focus of the contemporary photosynthesis biology
research. The International Symposium on Synthetic Biology in
Photosynthesis Research aims to bring together scientists working in the
various fields closely related to synthetic biology and photosynthesis research
areas to discuss the recent progress and brainstorm future opportunities. The
meeting dates shall hold from August 8 through August 10 in the Lake Meilan
International Convention Center.
The symposium will be organized around three topic areas, i.e.
understanding photosynthesis systems, engineering photosynthetic systems,
and new technologies/tools for synthetic biology. Plenty of time will be
allocated for the discussion sessions to ensure close interaction between the
speakers and audiences. At the end, we will organize a discussion session to
brainstorm unexplored opportunities on photosynthesis synthetic biology,
methods and tools needed to realize these opportunities, and maximize the
impact of photosynthetic synthetic biology on agriculture.
We look forward to having your participation at the meeting in the Aug of
2018!
1 Organizers
1.1 Supported by
Center of Excellence for Molecular Plant Science, CAS
Shanghai Institute of Plant Physiology and Ecology, SIBS, CAS
The ARC Centre of Excellence for Translational Photosynthesis, ANU
State Key Laboratory of Hybrid Rice
Chinese Society for Plant Biology
3
1.2 Sponsored by
The ARC Centre of Excellence for Translational Photosynthesis, ANU
European Union Europe Aid SEW‐REAP project
State Key Laboratory of Hybrid Rice
Chinese Society for Plant Biology
1.3 Organized by
Center of Excellence for Molecular Plant Science, CAS
Shanghai Institute of Plant Physiology and Ecology, SIBS, CAS
Chinese Society for Plant Biology
2 Registration Information
Students and postdocs: 2000 RMB; Faculty and staff: 3000 RMB.
Fees will support the Symposium material, tea break, meeting venue, and
invited speakers etc. The cost for accommodation and transportation need to be
covered by participants.
3 Registration
Please register for the meeting at http://meeting.cspb.org.cn/sbpr/ before
July 1st.
4 Registration fee payment options Bank transfer, Alipay, Weichat, or onsite payment by card for public service
(公务卡).
5 Bank transfers Beneficiary’s Name: The Chinese Society for Plant Biology
Bank Information: Agricultural Bank of China, Shanghai Municipal Branch,
Xuhui District, Fenglin Road Branch
Account No: 03392400801023728
SWIFT BIC: ABOCCNBJ090
Attention: When you pay for the meeting, please label clearly:
Photosynthesis Symposium + YOUR NAME
Contact for fee payments: Yajie Zheng, [email protected],021-54922857
Chinese Society for Plant Biology
August 1, 2018
4
Warm Reminder
Dear Sir or Madam:
Thank you so much for coming to Shanghai (International center for
economic, financial, trade, shipping, scientific and technological innovations)
in this summer to attend the International Symposium on Synthetic Biology
in Photosynthesis Research, which is co-sponsored by the ARC Centre of
Excellence for Translational Photosynthesis of ANU, State Key Laboratory of
Hybrid Rice and Chinese Society for Plant Biology.
For the success of symposium, please carefully read the following:
1 Hotel and Transportation
The hotel you are going to stay is Lake Meilan International Convention
Center of Shanghai, which is situated at No. No. 6655 Hutai Road, Baoshan
district, Shanghai (No. 888 Luofen Road, Luodian new town). It takes about 45
minutes taxi ride to reach Shanghai Hongqiao International Airport and
Shanghai Hongqiao Railway Station, 50 minutes to Shanghai Railway Station
and 75 minutes to Shanghai Pudong International Airport, 70 minutes to
Shanghai Institute of Plant Physiology and Ecology, SIBS, CAS.
The symposium will be held in Lake Meilan International Convention Center,
which locate only 600 meters away from “Meilan Lake Station” of Metro Line
7, which is about 10 minutes’ walk. The subway system can be reached from
Hongqiao airport, Pudong Airport, Shanghai Hongqiao Railway station,
Shanghai Railway station and Shanghai South Railway Station.
2 Procedure for Symposium Registration
2.1 Sign in (Please check your basic contact information so that the Symposium
5
Organizing Committee can keep contact with you and make the symposium
address list)
2.2 Receive your information pack and souvenir
2.3 Check in at Hotel Reception
3 Meals
The meal tickets grant you to have your meals during the symposium.
3.1 Breakfast (At 7:00-9:00; Western Restaurant, The first floor in hotel)
3.2 Lunch (At 12:00-13:30; Meilan Restaurant, The 3rd floor in hotel)
3.3 Dinner (At 18:00-20:00; Meilan Restaurant, The 3rd floor in hotel)
3.4 Banquet (At 18:30-21:00; All participants for the symposium will be invited
to have a banquet by the sponsors on Meilan Restaurant, The 3rd floor in
hotel)
4 Hotel Telephone Services
4.1 Interior Phone: Please dial 6+ room number.
4.2 Local Call: Please dial 9+ phone number.
4.3 Long Distance Call: Please contact Hotel information desk.
5 Weather Forecast
Shanghai (August 7 - 10)
Date Weather Temperature (℃)
August 7 Cloudy 26~33
August 8 Sunny 25~33
August 9 Cloudy 26~33
August 10 Sunny 26~33
6 Symposium Organizing Committee
(1) Team leaders
Prof. Xinzhuang Zhu (Center of Excellence for Molecular Plant Science,
CAS; Tele: 86-139 1705 8786)
6
Ms. Yajie Zheng (Chinese Society for Plant Biology; Tele: 86-188 1739
5069)
Prof. Jianfeng Cheng (Shanghai Institute of Plant Physiology and Ecology,
SIBS, CAS; Tele: 86-159 0096 7950)
(2) Rooms for organizing committee and interior phone number
Room Number Interior Phone Number Contact Person
Xinzhuang Zhu
Yajie Zheng; Li Zhou
Jianfeng Cheng
Qingfeng Song; Mingnan Qu
(3) Contact Person and Phone Number
①. Receipt for registration fee and company exhibition
Ms. Yajie Zheng; Tele: 86-188 1739 5069;
Ms. Li Zhou; Tele: 86-188 1739 5069;
②. Symposium program, venue and posters
Dr. Qingfeng Song; Tele: 86-135 6408 2434;
Dr. Mingnan Qu; Tele: 86-130 2210 8559;
Dr. Tiangen Chang; Tele: 86-139 1716 6684;
Dr. Honglong Zhao; Tele: 86-189 3991 0730.
7. Attentions
7.1 Please feel free to ask for any help from our staff wearing employee’s T-
shirts and cards.
7.2 Please the participants should be present at the symposium and attend
relevant activities on time and in an organized way according to the
schedule in symposium handbook. If there are any changes, please refer to
the on-site notices.
7.3 Please keep your cell phones opened. If you go out alone during the
7
symposium, please inform the symposium organizing committee. If there
is a need, please contact with the symposium organizing committee.
7.4 Please wear your participation card when you are present at the symposium,
attend relevant activities or have meals.
7.5 Please turn off your mobile phones or other communication tools or keep
them in silent mode and do not walk around at the symposium venue.
7.6 Please keep your own property and relevant documents properly and take
the room key/card with you when leaving the room; if something is lost,
please contact with the symposium organizing committee in time.
7.7 please contact with the symposium organizing committee about the matters
of printing, using cars, consulting or emergency during the symposium.
7.8 Please comply with the relevant regulations of the hotel, take good care of
the hotel facilities, keep the environmental health; if there is any damage,
and need to pay for the liability; and the all expenses of consumption shall
be paid by yourself. Please check out when you leave the hotel.
7.9 During the symposium, please do a good safe workings such as fire
prevention, electricity usage, food safety and good health; Smoking is
strictly prohibited in the venue and indoor areas.
7.10 The schematic diagrams of hotel location and transportation are shown on
the back cover.
8
Symposium Schedule
The Symposium lasts about four days (from August 7th to August 10th,
2018). All content arrangements are detailed below.
Date Time Content
August 7 01:00-09:00 PM Arrivals
August 8
08:30-09:00 AM Opening remarks
09:00-12:00 AM Session 1: Photosynthesis systems
01:30-05:00 PM Session 2: Engineering photosynthetic
systems
06:30-08:00 PM Symposium banquet
08:00-09:00 PM After banquet presentation
August 9
08:30-09:00 AM Poster viewing and discussions
09:00-12:00 AM Session 3: Tools sets for synthetic
biology in photosynthesis
01:30-04:40 PM Session 4: Discussion sessions
04:40-05:10 PM Symposium close
August 10 07:00-12:00 AM Symposium adjourn
9
Scientific Program
Symposium location: Symposium Room Number 2, The Third Floor,
Lake Meilan International Convention Center.
Tuesday, August 7, 2018: Arrivals
Afternoon
01:00-09:00 Registration open in the lobby of hotel
01:00-05:00 Social interaction, poster hanging and exhibition layout
06:00-08:00 Dinner (Meilan Restaurant, The third floor in hotel)
Wednesday, August 8, 2018: Full-day Sessions
Breakfast: 7:00-9:00 (Western Restaurant, The first floor in hotel)
Morning sessions (Chair: Xinguang Zhu)
08:30-08:50 Opening remarks by meeting organizers
08:50-09:00 Opening remarks by institute representative
Session 1 Photosynthesis systems (Chair: Susanne von Caemmerer)
09:00-09:30
Donald R. Ort (University of Illinois at Urbana-Champaign, USA)
Improving Photosynthetic Efficiency for Improved Crop
Yield
09:30-10:00 Kevin Griffin (Columbia University, USA)
Respiratory Metabolism during Photosynthesis
10:00-10:30 Asaph Cousins (Washington State University, USA)
Mesophyll CO2 Conductance in C3 and C4 plants
10:30-11:00 Coffee break
10
11:00-11:30
Yu Wang (University of Illinois at Urbana-Champaign, USA)
Variations of C4 Photosynthetic Pathways and Their
Physiological Significance
11:30-12:00
Tomas Brutnell (Danforth Plant Science Center, USA; Shandong
Agricultural University)
Re-engineering the Carbon Shuttle Pathway in Setaria viridis
12:00-13:30 Lunch (Meilan Restaurant, The 3rd floor in hotel)
Afternoon
Session 2 Engineering photosynthetic systems (Chair: Donald R. Ort)
01:30-02:00
Paul South (University of Illinois at Urbana-Champaign, USA)
Engineering Photorespiration: A Synthetic Biology
Approach to Improving Crop Productivity
02:00-02:30 Andrea Bräutigam (Heinrich-Heine-Universität Düsseldorf, Germany)
Modeling C4 Photosynthesis and Moonlighting in CAM
02:30-03:00
Ben Long (Australian National University, Australia)
How to Build a Carboxysome: Progress and Future
Challenges to Constructing Functional Carboxysomes in the
Chloroplast
03:00-03:30 Photograph and coffee break
03:30-04:00 Maria Ermakova (Australian National University, Australia)
Electron Transport in C4 Plants
04:00-04:30 Ron Milo (Weizmann Institute of Science, Israel)
Lessons from Synthetic Engineering of Carbon Fixation
04:30-05:00
Martin Parry (Lancaster University, UK)
Reconstitution of the Cyanobacterial Carbon Concentrating
Mechanism in Rice Chloroplasts to Improve Yield
05:00-06:00 Poster presentation from participants
06:00-06:30 Relaxation, happy hour
11
06:30-08:00 Symposium Banquet (Meilan Restaurant, The 3rd floor in hotel)
08:00-09:00
After dinner presentation (Meilan Restaurant, The 3rd floor in hotel)
Susanne von Caemmerer (Australian National University, Australia)
Novel Insights into the Regulation of C4 Photosynthesis
Thursday, August 9, 2018: Full-day Sessions
Breakfast: 7:00-9:00 (Western Restaurant, The first floor in hotel)
Morning
08:30-09:00 Poster viewing and discussions with coffee and beer provided
Session 3 Tools sets for synthetic biology in photosynthesis (Chair:
Martin Parry)
09:00-09:30
Yi Yang (East China University of Science and Technology, China)
Probing Cellular Redox Metabolism Using Genetically
Encoded Fluorescent Sensors
09:30-10:00 Fangfang Ma (Shandong Agricultural University, China)
Flux Analysis of Arabidopsis Primary Metabolism
10:00-10:30
Yi Xiao (Shanghai Institute of Plant Physiology & Ecology, Chinese
Academy of Sciences, China)
Three-dimensional Modeling of Rice Photosynthesis
10:30-11:00 Coffee break
11:00-11:30
Chen Yang (Shanghai Institute of Plant Physiology & Ecology,
Chinese Academy of Sciences, China)
Understanding and Manipulating Metabolic Fluxes in
Cyanobacteria
11:30-12:00
Stephanie Arrivault (Max Planck Institute of Molecular Plant
Physiology, Germany)
Metabolite Flux Analysis in Maize
12:00-13:30 Lunch (Meilan Restaurant, The 3rd floor in hotel)
12
Afternoon
Session 4 Discussion sessions (Chair: Xinguang Zhu)
Topic 1
01:30-02:00
New Opportunities for synthetic biology in photosynthesis
research. What are the major targets? How to identify new
targets?
02:00-02:10 Synthesis of the discussion and action plan
Topic 2
02:10-02:40
What is the ideal wish list to phenotyping a cell with an
engineered increased photosynthetic efficiency? Flux
estimate? Metabolite imagining? Cell imagining? CO2
pluming in a cell and leaf?
02:40-02:50 Synthesis of the discussion and action plan
02:50-03:20 Coffee break
Topic 3
03:20-03:50 How to expedite the photosynthesis synthetic biology to
maximize its impact?
03:50-04:10 Synthesis of the discussion and action plan
Topic 4
04:10-04:40 Panel discussion with leading experts answering any
questions from participants
04:40-05:10 Symposium close
06:00-08:00 Dinner (Meilan Restaurant, The 3rd floor in hotel)
Friday, August 10, 2018: Symposium Adjourn
Morning
07:00-09:00 Breakfast (Western Restaurant, The first floor in hotel)
09:00-12:00 Checkout and Return
13
Abstracts of
Invited Talks
14
Improving Photosynthetic Efficiency for Improved Crop Yield
Donald R. Ort
Departments of Plant Biology and Crop Sciences & Carl R. Woese Institute for Genomic Biology,
University of Illinois, Urbana-Champaign, IL, 61801, USA
Abstract: Feeding the world’s current population already requires 15% of the total net
primary productivity of the globe’s land area and that will need to increase to 25% in order
to meet the projected increase in agricultural demand this century. This near doubling of food
production will have to be accomplished on globally declining acreage and during a time in
which there will be ever increasing demand on cultivated lands for the production of
bioenergy crops, while in the face of a changing global environment that has already resulted
in decreasing global yield of some of the world’s most important food crops. The yield
potential of crops is determined by their efficiency of capturing available light energy (i),
the efficiency of converting intercepted light into biomass (c), and the proportion of biomass
partitioned into grain (η). The remarkable yield gains of the Green Revolution in the middle
of the 20th century resulted from plant breeders bringing η and i for major crops close to
their theoretical maxima, leaving improved photosynthetic efficiency as the only yield
determinant with sufficient capacity to double crop productivity. Opportunities to improve
photosynthetic efficiency exist in readapting photosynthesis to the rapid changes in
atmospheric composition and temperature, in redesigning photosynthesis for agricultural
production and in applying synthetic biology to bypass evolutionary limitations and
inefficiencies in photosynthesis.
Key words: Yield potential, net primary productivity, evolutionary limitations
15
Respiratory Metabolism during Photosynthesis
Kevin L. Griffin
Departments of Earth and Environmental Sciences and Ecology, Evolution and Environmental Biology,
Columbia University, Palisades, NY, 10964, USA
Abstract: Total plant CO2 fixation by photosynthesis is not a sufficient basis to predict
growth or ecosystem carbon cycling since respiration and other metabolic processes must
also be considered. Recent estimates conclude that neglecting these may underestimate the
yield from a given amount of CO2 assimilated by up to 30%. Furthermore, respiration plays
a supports nutrient assimilation which often occurs in illuminated leaves. Unfortunately,
modelling and predicting the carbon flux from day respiration remains a difficult exercise.
Moreover, independently determining the rates of photosynthesis and respiration in the light
is a fundamental challenge in plant physiology. We have developed a new method to
accurately measure gross and net oxygen production as a routine gas exchange measurement
with isotope ratio mass spectrometry. The results will be discussed in the context of
evaluating the technique presented as a unique tool to study and understand leaf
physiological traits. I will then briefly consider the ecological implications of the inhibition
of respiration in the light on carbon exchange from arctic ecosystems as measured by eddy
co-variance.
Key words: Respiration, oxygen isotopes, pyruvate dehydrogenase, DCMU
16
Mesophyll CO2 Conductance in C3 and C4 plants
Asaph B. Cousins
School of Biological Sciences, Washington State University, Pullman, WA, 99164, USA
Abstract: Diffusional limitations to carbon dioxide (CO2) movement into and within a leaf
results in reduced CO2 availability at the site of carboxylation and can therefore limit rates
of photosynthesis. The initial resistance of CO2 movement by stomata from the leaf surface
to the intercellular air spaces is well characterized and is known to strongly influence rates
of photosynthesis. However, within the leaf CO2 must further diffuse to the site of the initial
site of carboxylation, often referred to as mesophyll CO2 conductance (gm). In C3 plants the
initial carboxylation occurs within the mesophyll chloroplast via Rubisco and in C4 plants
this occurs in the mesophyll cytoplasm by PEP carboxylase. Measurements of gm show that
it varies between species and responds to short-term changes in measurement temperatures.
Data will be presented on how changes in cell wall properties influence gm and comparisons
in the temperature response of gm in C3 and C4 plants.
Key words: Mesophyll CO2 conductance, C3 and C4 photosynthesis, Cell walls
17
Variations of C4 Photosynthetic Pathways and Their
Physiological Significance
Yu Wang
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign. 1206 West
Gregory Drive, MC-195 Urbana, IL 61801 USA
Abstract: With a CO2 concentrating mechanism, C4 photosynthesis has higher energy use
efficiency than C3 photosynthesis. Historically C4 photosynthesis is classified into three
subtypes based on the main decarboxylation enzymes. However, recent experimental and
theoretical studies suggest that C4 pathways could be mainly categorized into two subtypes,
i.e. NAD-ME or NADP-ME subtypes with PEPCK as supplemental decarboxylases. The
CO2 concentrating mechanism of C4 photosynthesis requires a coordination of metabolic and
anatomical features. Besides differences in metabolism, NADP-ME and NAD-ME subtypes
have specialized leaf anatomies. However, the major physiological significance of
coordination between metabolic and anatomical features has not been systematically
evaluated. To quantify the impact of each features, we developed a generic dynamic systems
model of C4 photosynthesis where three different decarboxylases are represented
simultaneously together with variations of leaf anatomical and photosystem features. This
model can be degenerated to form different modes of C4 photosynthesis with different
combinations of decarboxylation pathways and anatomies. With this model, we show that
centrifugal chloroplast location and existence of cell wall suberin in bundle sheath cell are
required to gain high photosynthetic rate in monocot NADP-ME type C4 photosynthesis. In
contrast, neither chloroplast location nor existence of suberin is obligated to gain high
photosynthetic rate in NAD-ME type C4 photosynthesis. That is, natural combinations of
anatomical and metabolic features in monocot NADP-ME and NAD-ME plants confer
advantage in terms of photosynthetic CO2 uptake rates. It suggests that the typical
combinations reflect a certain degree of optimization for CO2 uptake, and different
combinations of anatomical and biochemical features can achieve to similarly high
photosynthetic rates.
Keyword: C4 Photosynthesis, energy use efficiency, three decarboxylation pathways, bundle
sheath chloroplast location, suberin
18
Re-engineering the Carbon Shuttle Pathway in Setaria viridis
Thomas P. Brutnell
College of Agronomic Sciences, State Key Laboratory of Crop Biology, Shandong Agricultural
University, 61 Daizong Street, Tai’an, Shandong 271018, China
Abstract: Setaria viridis is rapidly emerging as the premier model system for studies of C4
photosynthesis in the grasses. With a rapid life cycle of just 6 to 8 weeks, a short stature that
is similar to A. thaliana and relatively simple growth requirements, S. viridis is an attractive
system for conducting forward and reverse genetic screens to probe the genetic networks
underlying the function and regulation of C4 photosynthesis. To exploit Setaria as a model
system, we have been developing a number of genetic and genomics tools, methods and
resources for the community. Here I will present on methods we have developed to rapidly
identify candidate genes underlying phenotypes of interest and to identify putative rate
limiting steps in C4 photosynthesis through cross-species selection scans. I will also discuss
new methods and approaches to engineering synthetic circuits in plants and discuss how
these methods could be applied to altering metabolic flux in a C4 system as well as
engineering C4 traits into C3 systems.
Key words: C4 photosynthesis, genetics, synthetic biology, genomics, trait development
19
Engineering Photorespiration: A Synthetic Biology Approach to
Improving Crop Productivity
Paul F. South1,2 Amanda P. Cavanagh2 Helen W. Liu2, Donald R. Ort1,2
1 USDA-ARS Photosynthesis Research Unit, 2 Institute for Genomic Biology, University of Illinois,
Urbana-Champaign, IL, 61801, USA
Abstract: Worldwide nearly 1 billion people are affected by hunger every day. As climate
changes globally and human population increases, traditional methods of crop improvement
have become less effective in adapting and improving agricultural production. In C3 crops
such as wheat and rice approximately 25% of the fixed carbon dioxide is lost to
photorespiration. Photorespiration is an energy expensive metabolic pathway that recycles
toxic compounds produced by RubisCO oxygenation reactions. Reducing photorespiratory
yield losses by 5% (i.e., to 31% for soybean and 15% for wheat) would be worth millions
annually. Although photorespiration is tied to other important metabolic functions, the
benefit of improving its efficiency appears to outweigh any potential secondary
disadvantages. Synthetic biology has provided new opportunities in altering photorespiratory
metabolism to improve photosynthetic efficiency. Indeed, metabolic bypasses to
photorespiration have been generated and have demonstrated improvements in growth.
Using a synthetic biology approach, we have assembled a series of multigene constructs that
contain alternate metabolic pathways to photorespiration. In addition, we designed a screen-
based approach to test a range of standardized parts (promoters, terminators) in the model
plant Nicotiana tabacum. We have successfully transformed in large multigene constructs
and have demonstrated metabolic alternatives to photorespiration with significant
improvements in biomass in replicated greenhouse and field trails. Determining robust
alternative photorespiratory pathways can provide insight into next generation crops and our
utilization of standard parts provide a new tool kit for plant synthetic biology to engineer
improvements in photosynthetic efficiency.
Key words: Photorespiration, glycolate, multi-gene construct design, golden gate cloning
20
Modeling C4 Photosynthesis and Moonlighting in CAM
Andrea Bräutigam
Computational Biology, Faculty of Biology, Bielefeld University, Bielefeld, 33615, Germany
Abstract: Construction of models for metabolic pathways allow studying the
interconnections between phenotypes and metabolic types and the evolution of metabolic
pathways. I will present three modeling approaches which represent different concepts to
modeling carbon concentration pathways and their application to theoretically test
hypotheses. Kinetic modeling of the C4 cycle is used to elucidate the metabolic root of
chloroplast dimorphism. Stoichiometric modeling probes the origin of the C4 cycle and the
evolutionary choice of decarboxylation enzyme. Schematic models trace CAM back to the
synthesis of carbon backbones for amino acid synthesis in C3 species. I will present the
construction rational for the models, detail the hypotheses that we tested, and present the
conclusions for each of the models.
Key words: Modeling, chloroplast dimorphism, evolution of CCMs
21
How to Build a Carboxysome: Progress and Future Challenges
to Constructing Functional Carboxysomes in the Chloroplast
Ben Long
Plant Science Division, Research School of Biology, College of Science,
The Australian National University, Canberra ACT 2601, Australia
Abstract: The inclusion of a cyanobacterial CO2 concentrating mechanism (CCM) in C3
plant chloroplasts is a long-term goal predicted to significantly enhance photosynthetic
efficiency and yield. The system is bipartite, having an obligatory requirement for stromal
bicarbonate accumulation by protein pumps, and a specialized compartment for Rubisco
known as the carboxysome. The carboxysomes is a proteinaceous ‘organelle’ with a
selectively porous protein shell encapsulating high catalytic turnover Rubisco enzymes.
Carboxysome biogenesis is complicated, requiring the coordinated expression of around a
dozen proteins in some cases, and their correct organization into a large, functional mega-
complex. In addition, there are two distinct types of carboxysomes encapsulating either
Form1A or Form1B Rubisco, each with their advantages and disadvantages. Noting that a
chloroplastic CCM is reliant primarily on raising the stromal bicarbonate concentration,
achieving the construction of a functional carboxysome in a C3 plant chloroplast to make use
of the bicarbonate pool is a complex engineering challenge. Progress toward the assembly
of carboxysomes in tobacco chloroplasts will be presented, and future directions discussed.
Keywords: Carboxysomes, Rubisco, CO2 concentrating mechanism, chloroplast
22
Electron Transport in C4 Plants
Maria Ermakova1, Furbank Robert1, 2, Susanne von Caemmerer1
1 ARC Centre of Excellence for Translational Photosynthesis, Australian National University, Canberra,
Australia. 2 CSIRO Agriculture, Canberra, Australia
Abstract: Recent activities to improve photosynthetic performance in crop plants have
focused primarily on C3 photosynthesis where there are clear identified targets such as
improving Rubisco kinetics, installation of a CO2 concentrating mechanism and alleviating
limitations in chloroplast electron transport. However, C4 plants that utilize the C4
photosynthetic pathway also play a key role in world agriculture and strategies to manipulate
and enhance C4 photosynthesis thus have potential for major agricultural impacts. The C4
photosynthetic pathway is a biochemical CO2 concentrating mechanism that requires the
coordinated functioning of mesophyll (M) and bundle sheath cells (BS) of leaves and species
have evolved a complex blend of anatomy and biochemistry to achieve this. Chloroplast
electron transport in C4 plants is shared between these two cell types and the diversity of
thylakoid protein complexes of each cell type is defined by the requirements of the metabolic
sub-type of C4 photosynthesis. Our recent work with the model monocot C4 species Setaria
viridis (green foxtail millet) and transgenic S.viridis plants with altered amount of
cytochrome (Cyt) b6f complex demonstrates the link between electron transport capacity of
the leaves and CO2 assimilation. Overexpression of the Cyt b6f in both M and BS allows
higher rates of assimilation in transgenic plants without affecting Rubisco content. However,
increasing the amount of the Cyt b6f only in M, surprisingly, leads to a reduced rate of CO2
assimilation at low CO2. We link this observation to measurements of electron transport
components and light harvesting capacity of BS.
Keywords: Electron transport, C4 plants, photosynthesis
23
Lessons from Synthetic Engineering of Carbon Fixation
Ron Milo
Dept. of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Abstract: Can a heterotrophic organism be evolved to synthesize biomass directly from CO2?
In this talk, I demonstrate how a combination of rational metabolic rewiring, recombinant
expression and laboratory evolution has led to the biosynthesis of sugars and other major
biomass constituents, by a fully functional Calvin-Benson-Bassham cycle in E. coli. I will
describe the genetic basis for the adaptation of E. coli to sugar synthesis from CO2. We find
that only five mutations are sufficient to enable robust growth. All mutations are found either
in enzymes that affect the efflux of intermediates from the autocatalytic CO2 fixation cycle
towards biomass (prs, serA, pgi), or in key regulators of carbon metabolism (crp, ppsR).
Using suppressor analysis, we show that a decrease in catalytic capacity is a common feature
of all mutations found in enzymes. These findings highlight the enzymatic constraints that
are essential to the metabolic stability of autocatalytic cycles and can be relevant to future
efforts in constructing non-native carbon-fixation pathways.
Key words: Carbon fixation, auto-catalytic cycles, synthetic biology, Rubisco,
24
Reconstitution of the Cyanobacterial Carbon Concentrating
Mechanism in Rice Chloroplasts to Improve Yield
Page M T1, Qu M2, Perveen S2, Hanson M R3, Zhu X2, Lin M T3, Orr D J1,
Carmo-Silva E1 and Parry M A J1
1 Lancaster Environment Centre, Lancaster University, UK
2 Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, China
3 Department of Molecular Biology and Genetics, Cornell University, USA
Abstract: The challenge of feeding the world in the face of climate change requires
innovative ways of improving crop production. We are using a synthetic biology approach
to improve the assimilation of carbon from atmospheric CO2 by Rubisco. This enzyme
evolved around 3 billion years ago under very different atmospheric conditions. Its catalytic
properties, particularly the competing oxygenation of the substrate RuBP, limit
photosynthesis in higher plants in today’s atmospheric concentrations of CO2 and O2.
Cyanobacteria have evolved a carbon concentrating mechanism (CCM) which serves to
concentrate CO2 around Rubisco, mimicking the primitive atmosphere and allowing
cyanobacteria to utilize a Rubisco that performs carboxylation at much faster rates and
requires less investment in Rubisco. Modelling suggests that introducing the cyanobacterial
‘carboxysome’ micro-compartment into higher plants alongside other CCM components
may improve carbon assimilation by as much as 60%. We have previously introduced a faster
cyanobacterial Rubisco and components of the carboxysome shell into the model plant
tobacco. We now aim to increase the photosynthetic efficiency of rice by engineering the
essential components of a functional cyanobacterial CCM into rice chloroplasts. We have
employed the Golden Gate modular cloning system (‘MoClo’) to build a large library of
parts including promoters, transit peptides, terminators, fluorescent tags, and the
cyanobacterial carboxysome genes. We have coupled this cloning system, which enables the
rapid assembly of multigene expression cassettes, to a high-throughput transient expression
assay in rice protoplasts. We have then exploited this complete design-build-test cycle to
confirm chloroplast targeting of carboxysome components through confocal microscopy, and
to examine the effect of altering the ratio of carboxysome components on protein aggregation.
In addition, we have assembled and tested a plasmid with ten expression cassettes to express
all essential carboxysome genes. Promising plasmids from the design-build-test cycle have
been used to generate stably transformed rice, and the resulting plants are expected to have
substantially higher yields and show improvements in both water and nitrogen use
efficiencies.
Key words: Carboxysome, Cyanobacteria, rice, tobacco, MoClo
25
Novel Insights into the Regulation of C4 Photosynthesis
Susanne von Caemmerer1, Jasper Pengelly1, Florence Danila1, Maria
Ermakova1, Hannah L Osborn1, Hugo Alonso-Cantabrana1, Rosemary
White1,2, Robert T Furbank1
1 ARC Centre of Excellence for Translational Photosynthesis, Australian National University, Canberra,
Australia; 2 CSIRO Agriculture and Food, Canberra, Australia
Abstract: High photosynthetic rates of C4 plants are due to a biochemical CO2 concentrating
mechanism that requires coordination of leaf mesophyll (M) and bundle sheath (BS) cells.
The complexity of this cellular specialisation has hindered our understanding and prevented
the development of strategies to improve photosynthetic rates of C4 species. We have used
molecular techniques to study this regulation and generated a number of transgenic plants in
Flaveria bidentis (a C4 dicot) and Setaria viridis (a C4 monocot) where the photosynthetic
metabolism has been altered with RNAi, antisense or overexpression constructs to silence
or enhance expression of various photosynthesis-related genes. We have used tuneable diode
laser absorption spectroscopy to make concurrent measurements of carbon isotope
discrimination and gas exchange to evaluate the photosynthetic efficiency and mesophyll
and bundle sheath interaction. High C4 photosynthetic rates require high metabolic fluxes
between mesophyll and bundle sheath cells, through interconnecting plasmodesmata (PD),
to support the C4 biochemical CO2 pump. We have developed a new quantitative technique,
which combines scanning electron microscopy (SEM), and three-dimensional (3D)
immunolocalisation in intact leaf tissues to quantify plasmodesmatal (PD) density on
mesophyll bundle sheath cell interfaces. Our quantitative data are essential for modelling
studies and guide the development of synthetic biology strategies to manipulate and enhance
leaf photosynthesis in C4 species. They also underpin our understanding of the key
components needed to build C4 rice.
Key words: Flaveria bidentis, Setaria viridis, carbon isotope discrimination,
Plasmodesmata, scanning electron microscopy, 3D immunolocalisation.
26
Probing Cellular Redox Metabolism Using Genetically Encoded
Fluorescent Sensors
Yi Yang1,2
1 State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for
Biomanufacturing Technology, East China University of Science and Technology, Shanghai 200237,
China. 2 CAS Center for Excellence in Brain Science, Institute of Neuroscience, Chinese Academy of
Sciences, Shanghai 200031, China
Abstract: Oxidation-reduction reactions are not only central for cell metabolism, but also
integral components of cellular signaling and cell fate decision. Cellular redox states are
mainly governed by pyridine nucleotides (NADPH/NADP+ and NADH/NAD+), thiols and
reactive oxygen species (ROS), which form a complex network of interactions. It remained
challenging for many years to study cell redox metabolic states in situ and in real time. To
visualize their homeostasis and dynamics spatiotemporally, we and other groups developed
genetically encoded fluorescent sensors for NADH, the NADH/NAD+ ratio, NAD+, NADPH
and NADP+. Among them, the Frex, SoNar and iNap sensors are intensely fluorescent,
rapidly responsive genetically encoded sensors of wide dynamic range, which respond to
subtle perturbations of various pathways of energy metabolism in real-time. The Frex sensors
can be targeted to subcellular organelles and can be used to quantitate NADH concentrations
inside living cells, while SoNar and iNap sensors can be used or to track NAD+/NADH redox
states or NADPH level in living cells and in vivo. These genetically encoded sensor-based
metabolic screening could serve as a valuable approach for metabolism studies and drug
discovery.
Key words: Genetically encoded sensor, redox, live cell imaging, NADH, NADPH
27
Flux Analysis of Arabidopsis Primary Metabolism
Fangfang Ma1,2, Doug K. Allen3,4
1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, 271018,
China; 2 College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’An,
Shandong, 271018, China; 3 Donald Danforth Plant Science Center, St. Louis, MO, USA; 4 United
States Department of Agriculture, Agricultural Research Service, St. Louis, MO, USA
Abstract: A quantitative description of intracellular carbon fluxes via stable isotope labeling
and metabolic flux analysis (MFA) holds unique advantages in identifying pathway
bottlenecks and unfolding network regulation in biological systems, especially those that
have been engineered to alter their native metabolism. Isotopically nonstationary 13C
metabolic flux analysis (13C-INST-MFA), which is based on transient 13C labeling studies at
metabolic steady state, provides a comprehensive platform to quantify plant cellular
phenotypes. Being able to describe the full mass isotopomer distributions (MIDs) of
measured metabolites, this approach does not require direct measurements of pool sizes and
offers better flux resolution. Especially for autotropic tissues like leaves which cannot
maintain a metabolic steady-state for more than 12 hr, 13C-INST-MFA is a necessity for flux
estimation whereas uniform steady-state 13C-labeling is rather uninformative. Here I will
present the application of 13C-INST-MFA in quantifying Arabidopsis primary metabolism
responding to environmental (light and CO2) and/or genotypical perturbations. This strategy
allows us to comprehensively estimate a total of 136 fluxes including Calvin cycle,
photorespiration, sucrose and starch synthesis, tricarboxylic acid (TCA) cycle, and amino
acid biosynthetic fluxes. Our working hypothesis based upon our past studies in other plant
tissues is that integrative systems approaches are required to quantify the global impact of
specific perturbations on metabolic pathway fluxes and to guide further rounds of metabolic
engineering. Details such as the transient 13CO2 labeling of leaf tissue, sample handling,
mass spectrometry (MS) analysis of isotopic labeling data, and the computational flux
estimation using INST-MFA will be covered.
Key words: Metabolic flux analysis, 13C-labeling, primary metabolism, isotopomer
modeling
28
Three-Dimensional Modeling of Rice Photosynthesis
Yi Xiao
Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy
of Sciences, Shanghai, China
Abstract: Quantitative understanding to the impacts of biochemical and anatomical factors
is a key step of engineering a leaf photosynthesis system. The eleaf model, which is a highly
automatic three-dimensional (3D) mechanistic model of leaf photosynthesis, was developed
including a module of 3D reconstruction of leaf anatomy based on experimental data, a
module of light propagation simulating the non-uniform light environment inside the leaf, a
module of CO2 reaction-diffusion simulating the non-uniform CO2 environment inside the
leaf, and a module of photosynthetic metabolism which can either be a Farquhar-von
Caemmerer-Berry (FvCB) type of empirical model or a complex kinetic model such as Zhu
et al., 2007. Moreover, an extended FvCB framework of leaf photosynthesis was derived to
facilitate a mechanistic understanding of simulations or comparison studies from eleaf. This
new framework introduced six additional variables to the original FvCB model during the
calculation of leaf photosynthesis rate. Those variables represent the influence from the non-
uniform photosynthetic status of different cells and the coordination between profiles of light
absorptance, CO2, Vmax and Jmax. Oryza sativa IR64 grown under ambient CO2 (AC) and
elevated CO2 (EC) was modeled and compared with the new model and framework.
Photosynthesis metabolism plays a major role to the altered leaf physiology of higher net
photosynthesis rate. In addition, various anatomical factors also play significant impacts to
the altered leaf physiology, such as leaf thickness, vein density, mesophyll cell size,
chlorophyll amount. Although it seems not all of them contribute a positive effect to higher
photosynthesis rate, but their contributions to leaf photosynthesis are possibly mainly
achieved by regulating two physiological processes, which are 1) the coordination between
profile of Jmax; 2) the photosynthetic status of cells inside the leaf.
Key words: Photosynthesis, rice, 3D model, elevated CO2
29
Understanding and Manipulating Metabolic Fluxes in
Cyanobacteria
Chen Yang
CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences,
Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032,
China.
Abstract: Cyanobacteria are excellent models for studies of photosynthetic metabolism as
they lack the extensive compartmentalization of eukaryotes but retain much of the conserved
core of central metabolism. Understanding of how cyanobacteria adjust their metabolic
fluxes to enable adaptation to changes in nitrogen supply may help to improve nitrogen use
efficiency in plants. Recently, by combining dynamic 15N and 13C tracers, metabolomics, and
mathematical modeling approaches, we unraveled the mechanisms used by cyanobacteria to
cope with sudden nitrogen availability, leading to identification of an active ornithine-
ammonia cycle (OAC) in cyanobacteria. The pathway starts with carbamoyl phosphate
synthesis by the bacterial and plant type, glutamine-dependent enzyme and ends with
conversion of arginine to ornithine and ammonia by a novel arginine dihydrolase. We
demonstrated that the OAC allows rapid remobilization of nitrogen reserves upon starvation
and high rate of nitrogen assimilation and storage once the nutrient is available. Thus, the
OAC serves as a conduit in the nitrogen storage and remobilization machinery in
cyanobacteria and enables cellular adaptation to nitrogen fluctuations. Based on quantitative
knowledge of in vivo intracellular fluxes, we engineered the cyanobacterium Synechococcus
elongates to produce isoprene that is a key building block of synthetic rubber and currently
produced entirely from petrochemical sources. The engineered strain directed about 40% of
photosynthetically fixed carbon toward the isoprene biosynthetic pathway, resulting in the
production of 1.26 g L-1 of isoprene from CO2, which is a significant increase for terpenoid
production by photoautotrophic organisms. The constructed strains can be used to construct
a photoautotrophic cell factory for the production of diverse terpenoids from CO2.
Key words: Metabolic flux, cyanobacteria, terpenoid
30
Metabolite Flux Analysis in Maize
Stéphanie Arrivault
Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam-Golm,
Germany
Abstract: In plants performing C4 photosynthesis CO2 is incorporated into 4-carbon
metabolites in the mesophyll cells (MC) which move to the bundle sheath cells (BSC) where
they are decarboxylated to concentrate CO2 around RuBisCO. Historically C4 plants have
been classified into three biochemical subtypes based on the main enzyme involved in 4-
carbon metabolite decarboxylation: NADP-malic enzyme (NADP-ME), NAD-malic enzyme
or phosphoenolpyruvate carboxykinase (PEPCK). However, this is now recognized to be an
over-simplification as more than one decarboxylation pathway can operate in parallel within
the same leaf. Leaves of maize, which is classically considered to be an NADP-ME subtype
species, were supplied with 13CO2, quenched at various time intervals and mass
spectrometric methods used to determine the incorporation of 13C into 35 metabolites. These
included proposed intercellular transport metabolites (e.g. malate, aspartate, pyruvate,
alanine, phosphoenolpyruvate), as well as intermediates and products of the Calvin-Benson
cycle (CBC), tricarboxylic acid cycle, sucrose and starch synthesis, photorespiration and
amino acid metabolism. In addition, we obtained fractions enriched in MC and BSC from
13CO2-labelled material to determine intercellular distributions and concentration gradients
of metabolites. These analyses confirmed there is a concentration gradient of malate that can
drive diffusion from the MC to the BSC for decarboxylation by NADP-ME. They also
revealed intercellular concentration gradients of aspartate, alanine and phosphenolpyruvate
that could drive the metabolite transport associated with a PEPCK subtype shuttle, and that
it carries 10-14% of the carbon into the BSC in maize. There is also rapid carbon exchange
between the CBC and the CO2 concentrating shuttles, equivalent to about 10% of carbon
gain. We postulate that the presence of multiple shuttles, alongside carbon transfer between
them and the CBC, confers great flexibility in C4 photosynthesis, allowing maize to adjust
its photosynthetic metabolism in response to changes in light, temperature, nitrogen status
or other environmental factors.
Key words: Photosynthesis, maize, 13CO2 labelling kinetic
31
Abstracts of
Posters
32
No.01
Effect of Different Metals (Lead and Zinc) on Chlorophyll
Fluorescence in Black Gram and its Partial Recovery by
Brassinosteroid
Alok Srivastava, V.P. Singh
Department of Plant Science, M. J. P. Rohilkhand University, Bareilly U.P.
Abstract: The matle stress is found to induce negative influence on chlorophyll and
photosynthesis and it is recently found that plant growth regulators can mitigate these
negative impacts. A comparative study of effects of exposure to high lead (200 mg/kg-1) and
Zinc (400mg/kg) treatments have significantly retarded the growth of black gram plants
grown hydroponicaly, even at seven days both the metals inhibited the growth of plants by
about 40%. The CO2 assimilation was also significantly retarded. The chlorophyll a
fluorescence analysis showed that electron transport process was disturbed by this metal.
The inactivation of some parts of PS II was the main cause of these retardations. The
spescificity of mode of action of these matels showed that lead is more sensitive as compared
to Zinc. Although the mode of action of both the matels was found same. The use of
brassinosteroid (1ppm) significantly recovered the inibitions. The target molecule of this
regulator was BZR1 which was found to directly bind to the promoter reasons.
Key words: Metals (Lead and Zinc), chlorophyll fluorescence, black gram, brassinosteroid
33
No.02
Differential Responses of Mesophyll Conductance to
Temperature at Three Different O2 Concentrations in Rice
Plants
Guanjun Huang, Yong Li
Ministry of Agriculture Key Laboratory of Crop Ecophysiology and Farming System in the Middle
Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural
University, Wuhan, China
Abstract: Understanding the temperature response of mesophyll conductance (gm) is of
great importance for both photosynthesis models and crop production, especially with the
current global-warming climate. The temperature response of gm has been studied in many
plant species, but the underlying mechanisms are yet well known. Proteins, such as
aquaporins, are suggested to involve in this process (Bernacchi et al., 2002; Flexas et al.,
2008). A two-component model, which divides the mesophyll resistance into liquid and
membrane phases, are proposed to intercept the species-specific temperature responses
(Evans and von Caemmerer, 2013; von Caemmerer and Evans, 2015). Apparent gm is
recently hypothesized to be related to (photo)respiration (Tholen et al., 2012; Xiao and Zhu,
2017; Yin and Struik, 2017), which is temperature dependent. In the present study,
temperature response of gm was measured at three different O2 concentrations (10%, 21%
and 40%) in rice plants, to verify the hypothesis of the function of (photo)respiration on gm
and to investigate whether the effects of (photo)respiration on gm is temperature dependent.
The results showed that: (1) there was no significant difference for gm among three different
O2 conditions when leaf temperature (Tleaf) is no more than 25℃, when Tleaf ≥ 30℃, however,
gm at 40% O2 condition was significant lower than those at both 10% and 21% O2 treatments;
(2) in comparison with 10% O2, gm at 21% and 40% O2 was 14.3% and 42.9%, respectively,
less sensitive to temperature. This suggested that (photo)respiration has a great impact on gm,
especially at high temperature, and its response to temperature. Further researches should be
conducted to investigate whether leaf anatomy, such as chloroplast surface area, can impact
the function of (photo)respiration on gm.
Keywords: Mesophyll conductance, temperature, (photo)respiration
34
No.03
Cyclic Flectron Flow Can Protect PSII Against Photoinhibition
in Rice Following Heat Stress
Jemaa Essemine, Mingnan Qu, Genyun Chen, Xinguang Zhu
Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Feng Lin Road,
Shanghai 200032, China
Abstract: In a screening study performed in our lab on a global rice minicore panel, we have
identified two rice accessions characterized by their differential natural capacities in driving
cyclic electron flow (CEF) around PSI; i.e., low CEF (lcef) and high CEF (hcef) for C4023
and Q4149, respectively. A quick down-regulation in the PSI activity compared to that of
PSII has been reported following short-term heat stress in these two rice lines. Our results
demonstrated likewise that although the quick down-regulation of PSI; these two rice lines
have different protection mechanisms to photosystem II from photodamage under heat stress.
We observed a stepwise alteration in the shape of Chl a fluorescence induction (OJIP) with
increasing temperature treatment. The effect of 44°C treatment on the damping in Chl a
fluorescence was more pronounced in C4023 than in Q4149. We recorded as well a
disruption in the I-step, a decline in the Fv due to a strong damping in the Fm and a slight
increase in the F0. Normalized data demonstrate that I-step seems more susceptible to 44°C
in C4023 than in Q4149. We measured also the redox states of plastocyanin (PC) and P700
by monitoring the transmission changes at 820 nm (I820) and observed a disruption in the
oxidation/reduction kinetics of PC and P700. The decline in the amplitude of their oxidation
is shown to be about 29% and 13% for C4023 and Q4149, respectively. The electropotential
component (Δφ) of ms-DLE appears more sensitive to temperature stress than the chemical
component (ΔpH) and the impact of heat was more obvious and drastic in C4023 than in
Q4149. Under heat stress, we noticed a concomitant decline in the primary photochemistry
of PSII and in both the membrane energization process and the lumen protonation for both
accessions and it’s evident that heat affects more these parameters in C4023 than in Q4149.
All these data suggest that higher CEF may provide higher photoprotection to PSII in rice
leaves, which can be a desirable trait during rice breeding especially in the context of a
“warming” world.
Key words: Cyclic electron flow, fluorescence induction, heat stress, photoinhibition,
photosynthesis, rice
35
No.04
Seasonal Variations of Sun-induced Chlorophyll Fluorescence
from Leaf to Canopy Level and its Relations with Plant Traits
for Paddy Rice
Ji Li1, Yongguang Zhang1,2*, Qian Zhang1, Zhaohui Li1,
Jing Li1, Jingming Chen1
1 Jiangsu Provincial Key Laboratory of Geographic Information Science and Technology, International
Institute for Earth System Sciences, Nanjing University, 210023 Nanjing, China
2 Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and
Application, 210023 Nanjing, China
* Corresponding author, phone: +86-25-89681569, E-mail: [email protected]
Abstract: Sun-induced chlorophyll fluorescence (SIF) was the most potential probe of the
ecosystem photosynthesis. The consistency of SIF at leaf and canopy scales is an important
prerequisite of indicating photosynthesis with SIF at multiple scales. The correlations among
SIF, steady state fluorescence (Fs), maximum carboxylation rate of photosynthesis (Vcmax)
and chlorophyll content (Chla+b) are of great significance for understanding the mechanism
of relationships between SIF and photosynthesis. In this study, we investigated the
consistency of chlorophyll fluorescence parameters at leaf and canopy scales, and the
relationships between SIF and leaf traits (mainly Vcmax, Chla+b) throughout the growing
season based on the field measurements in a rice paddy. The rice growing season was divided
into two stages (Stage-I and Stage-II) with the flowering period as the boundary. The results
showed that, (i) SIF and Fs had significant consistency on the seasonal scale, especially in
Stage-II; SIF at leaf and canopy scales showed significant consistency in Stage-II; (ii) the
correlations between SIF and Chla+b (Vcmax) differed during Stage-I and Stage-II; (iii)
canopy structure dominated the difference of the relationships between SIF and leaf traits at
different scales, and the relationships between SIF and leaf traits affects the seasonal
estimation of photosynthesis based on SIF. These results made it possible for accurate
estimation of photosynthesis using SIF at multiple scales.
Key words: Sun-induced chlorophyll fluorescence, consistency, Vcmax, leaf chlorophyll
content, Anthesis stage, different scales
36
No.05
Sensitive Response of Chloroplast Size to Leaf Nitrogen Content
at the Tillering Stage Resulted in the Decreased Photosynthetic
Nitrogen Use Efficiency (PNUE) in Rice (Oryza sativa L.) Plant
Limin Gao
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center
for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste
Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China
Abstract: Previous studies demonstrated the decreased Photosynthetic Nitrogen Use
Efficiency (PNUE) under high nitrogen (N) supply was resulted from enlarged chloroplast
in rice (Oryza sativa L.) seedlings. To investigate the response of chloroplast size to nitrogen
supply at different rice growth stage and its role in regulating PNUE, pot experiments were
conducted with different nitrogen supply amounts. Our results showed the PNUE increased
with rice growth process and decreased with rising leaf N content, the most significant
differences among different growth stages in leaf N partitioning was observed in restored N,
which was positively correlated with Rubisco content. Both the gt/Rubisco and Cc/Rubisco
were significantly lower at the tillering stage than any other later stages. Besides, the
chloroplast surface area per leaf area (Sch) was significantly higher at the tillering stage,
during which the variation in Sch was much more sensitive to leaf N content. Therefore, we
concluded that the sensitive response of Sch to leaf N content at the tillering stage than other
stages resulted in the decreased gt/Rubisco and Cc/Rubisco, which was unable to satisfied
the carboxylation demand of Rubisco and induced the decreased PNUE ultimately.
Key words: Rice (Oryza sativa L.), chloroplast, leaf nitrogen content, photosynthetic
nitrogen use efficiency (PNUE)
37
No.06
Synthesis of Structural Carboxysomes in Tobacco Chloroplasts
WeiYih Hee
RIPE Lab, Plant Science Division, Research School of Biology, Linnaeus Building 134, Linnaeus Way,
Australian National University,Canberra, ACT 2601, Australia
Abstract: One promising approach to increasing photosynthetic efficiency and crop yield is
to incorporate the cyanobacterial CO2-concentrating mechanism (CCM) into crop plant
chloroplasts. Our studies focus on the structural formation of carboxysomes, one component
of the bipartite CCM, within tobacco chloroplasts. Formation of structural and functional
carboxysomes requires coordinated expression of around a dozen proteins, highlighting that
their transgenic construction is a complex engineering task. In this study, we successfully
synthesised simplified carboxysomes, derived from the source organism Cyanobium, within
tobacco chloroplasts. We replaced the endogenous Rubisco large subunit gene with a gene
cassette expressing cyanobacterial Form-1A Rubisco large and small subunits, and two key
α-carboxysome structural proteins, CsoS1A and CsoS2. Our results demonstrate the first
evidence of plant growth dependent on Form-1A Rubisco, as well as the successful
encapsulation of this Rubisco in chloroplastic carboxysomes. Technical detail and analysis
of transgenic carboxysomes is presented.
Key words: Carboxysomes, tobacco, chloroplasts, synthesis
38
No.07
Response of Photosynthetic Efficiency and NPQ based
Photoprotection of Rice Plants Grown under Different LED
Light Wavelength (Red, Blue and White)
Saber Hamdani, Naveed Khan, Shahnaz Perveen, Mingnan Qu, Jianjun
Jiang, Xinguang Zhu
State Key Laboratory for Plant Molecular Genetics, Center of Excellence for Molecular Plant Science,
Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China
Abstract: Non-photochemical quenching (NPQ) plays major role in regulating
photosynthesis and photoprotection in plants. Effect of light intensity on NPQ related
photoprotection has already been investigated comprehensively. However, the impact of
light quality on NPQ is scarce. Here, we studied how different wavelength of light (red, blue
and white) influences NPQ in rice using chlorophyll a fluorescence measurement together
with transcription analysis. Our results show that both blue and red light induced a
significantly higher NPQ accompanied by a cost of significant decrease in PSII quantum
efficiency as compared to white light. Furthermore, we found significant decrease in both
catalase (CAT) and ascorbate peroxidase (APX) transcript under blue and red light,
accompanied by an impairment of H2O2 detoxification. This suggest, plants grow under
monochromatic light may compromise its antioxidant system. Therefore, higher NPQ
capacity may also reflect the deficiency of the antioxidant system to cope with high light
stress. Our study also put a light on an additional role of white light that it may play for
effective photosynthesis in nature, however, this needs to be further investigated.
Key words: Antioxidant system, effective quantum yield of PSII, light quality, non-
photochemical quenching (NPQ), rice (Oryza sativa L.)
39
No.08
The Photosynthetic Responses of Panicum antidotale under
Salinity, Drought and Combination of both Stresses
Tabassum Hussain1, 2, Xiaojing Liu1
1 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese
Academy of Sciences, Shijiazhuang 050021, China
2 Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi-75270, Pakistan
Abstract: The combination of salt and drought is one of the common co-occurring stress
factors in arid and semi-arid areas. The present study was designed to mimic such conditions.
Panicum antidotale was grown in a green-house under treatments; control (without salt and
well-irrigated), salinity (100 and 300 mM NaCl), drought (30% irrigation) and combination
of salt and drought (100+D and 300+D: drought was achieved by 100 and 300 mM NaCl).
In general, all treatments caused a reduction in plant growth but 100 mM salinity remained
similar to control while 100+D stimulated biomass when compare to drought only. Drought
combination with high salinity couldn’t show positive relation as in low salinity but a slight
improvement in water relations. Gas exchange and chlorophyll a fluorescence were
expressed differentially either under salt and drought alone or combination of both. The net
photosynthesis (Pn) and other related parameters [stomatal conductance (gs), intercellular
CO2 (Ci), transpiration (E), Rubisco carboxylase activity (Vcmax), maximum electron
transport (Jmax), and triose-phosphate utilization (TPU)] were declined, the most, at 300+D
as compare to control while 100+D performed better as compare to drought only. The
quantitative analyses of photosynthetic limitation factors revealed that the most limitation
was contributed by biochemical limitation (Lb) as compare to stomatal (Ls) and mesophyll
limitations (Lm). The Lm was also supported by thylakoid reactions (chlorophyll a
fluorescence parameters). It can be concluded that the combination of low salinity with
drought was minimized deleterious effects of drought alone but 300+D treatment caused a
synergetic stress effect. This study also illustrated the quantitative disentangling Lb of
photosynthesis over Ls although intrinsic water use efficiency (WUEi) was enhanced due to
Ls that demonstrate water conservation ability of Panicum under water-deficit conditions
either due to salinity, drought and/or combination of these.
Key words: Water deficit, salt resistance, combine stress, photosynthesis, halophyte
40
No.09
Cryo-EM Structure of Maize PSI-LHCI-LHCII Supercomplex
Xiaowei Pan, Jun Ma, Xiaodong Su, Wenrui Chang, Zhenfeng Liu,
Xinzheng Zhang, Mei Li
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules,
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Abstract: Photosynthesis is one of the most amazing chemical reactions in the planet. The
light-driven electron transport of photosynthesis is accomplished by photosystems I and II
(PSI and PSII). In the natural environment, the fluctuating illumination can cause unequal
excitation of the two photosystems due to the different light absorption properties of their
antenna systems. Balanced light harvesting is crucial for efficient photosynthesis, and plants
have evolved sophisticated regulatory mechanisms in order to optimize the photosynthetic
efficiency and to avoid photo-damage. State transitions are one of the short-term adaptation
mechanisms. During state transitions, the trimeric LHCII is reversibly phosphorylated and
de-phosphorylated, and migrates between the two photosystems. Under light conditions
favoring PSII excitation, over-excitation of PSII leads to the activation of LHCII kinase and
subsequent phosphorylation of the N-terminal region of LHCII. A portion of the
phosphorylated LHCIIs move laterally within the thylakoid membrane from PSII to PSI,
forming the PSI-LHCI-LHCII supercomplex, increasing energy transfer towards PSI core.
We solved the cryo-electron microscopy (cryo-EM) structure of maize PSI-LHCI-LHCII
supercomplex at 3.3 Å resolution. Total of 21 protein subunits, 202 chlorophylls, 47
carotenoids and numerous other cofactors were identified in the final structure. Two PSI core
subunits (PsaN and PsaO) absent in the previously reported crystal structures were identified.
In addition, the phosphorylation site in LHCII was solved and the detailed interactions
between LHCII and PSI were revealed. The structure showed that PsaN and PsaO are at the
PSI-LHCI interface and the PSI-LHCII interface, respectively. Each subunit relays
excitation to PSI core through a pair of chlorophyll molecules, thus revealing previously-
unseen paths for energy transfer between the antennas and the PSI core.
Key words: Photosystem I, state transitions, structure
41
No.10
Low Level of HCO3- Content Involved in Drought Response in
Transgenic Rice with overexpression C4-PEPC
Zhang Jinfei1, 2, Li Xia1, *, Xie Yinfeng2
1 Jiangsu Rice Engineering Research Center, National Center for Rice Improvement (Nanjing), Jiangsu
Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China
2 College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China
*Corresponding author E-mail addresses: [email protected]
Abstract: Frequent occurrence of drought stress all over the world can lead to the stomata
closure in leaves of many plants, especially C3 plants such as rice and wheat, which affects
the flow of CO2 into plants and restricts growth and grain yield. C4-PEPC is a key enzyme
gene acting as “CO2 pump” to concentrate CO2 in C4 plant leaves and alleviate adverse
effects of stomatal closure on plant growth by HCO3-. Scientists have succeeded in
introducing the key photosynthetic gene of C4 plants into rice or other C3 plants with the
development of genetic engineering. The results showed that exogenous NaHCO3 can
enhance the drought response in PC rice lines with higher relative water content (RWC),
PEPC enzyme activity, PEPC gene expression and OsA1/2 (H-ATPase, OSA) gene
expression. On the contrary, drought treatment decreased the activities and gene expression
of the endogenous carbonic anhydrases (CA) such as bCA1/2 of two rice lines, but still
maintaining the higher level of PC lines as compared with WT. After the treatments of 100
μM exogenous carbonic anhydrase inhibitor (5-acetamido-1,3,4-thiadiazole-2-sulfonamide,
AZ) combined with 12% PEG, the difference in CA activities and the expression levels of
Os10g10470/Os12g03260 was eliminated between PC and WT plants. It is noteworthy that
the RWC of PC was still significantly higher than that of WT with higher levels of C4-PEPC
transcript and their protein content, and lower levels of SAPK9 as well after the same
treatment with AZ. The yeast two-hybrid test further confirmed that PEPC and SPAK9 had
no direct interaction. Moreover, treatments with AZ and 12% PEG6000 also eliminate the
difference of KAT1 (Arabidopsis K+ transporter 1 and KAT1) and SLAC genes expression
between PC and WT rice lines response to drought stress, indicated the drought response of
PC rice lines is independent of on the regulation of stomatal genes involved by the
endogenous carbon anhydrase. This study shown that PC was more sensitive to low level of
HCO3- content with the negative regulation of C4-PEPC gene and enzyme activity in rice
leaves, regulated stomatal movement, and conferred the drought tolerance of PC rice.
Key words: Rice, phosphate phosphoenolpyruvate carboxylase, carbonic anhydrases,
SnRKs, drought response
42
No.11
Concerted Decreases in Leaf Photosynthesis and Hydraulic
Conductance under K Deficiency: Prominent Roles of
Mesophyll Conductance to CO2 and Outside-xylem Hydraulic
Conductance
Zhifeng Lu, Shiwei Guo
College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing, China
Abstract: Potassium (K) starvation due to routinely apply of unbalanced fertilizers generally
causes retardation in plant growth and yield losses. Typical symptoms of K deficiency,
characterized as chlorosis at leaf tips which gradually evolving into withered necrosis and
sprawling to the center, is concomitantly occurred with downregulated leaf photosynthesis
(A) and broken leaf water homeostasis. However, to date, little is known about the prominent
limiting factors and their underlying mechanisms with respect to the concerted decreases in
A and leaf hydraulic capacity under conditions of K depletion. In this study, dicotyledonous
(cucumber, rapeseed) and monocotylous crops (rice, wheat) were investigated by providing
an overview of the responses of leaf A and hydraulic conductance (Kleaf), as well as their
limitations and corelated anatomical determinants to three different K regimes under their
own growth conditions. Leaf total (VLA) and minor vein density (VLAminor) of monocot
species were improved under K deficiency, while opposite effects were observed in dicot
species. Potassium starvation concertedly limited leaf A and Kleaf, of which the former was
determined mainly by the mesophyll CO2 diffusion resistance (contributing to 50.9% of total
limitations), and the latter by outside-xylem hydraulic resistance (accounting for 60.2% of
enhanced resistance). Leaf A and Kleaf were closely related to anatomical traits (e.g. VLAminor
and the surface area of chloroplasts that exposing to intercellular airspaces), particularly
those in dicotyledonous crops. The two main components (i.e. gm and Kox) were tightly
coupled, ascribing to the shared pathways of CO2 and H2O transport. These results
emphasize the important role of K on the regulation of concerted changes of A and Kleaf by
modifications in leaf anatomy.
Key words: Leaf photosynthesis, leaf hydraulic conductance, mesophyll conductance, leaf
vein density
43
No.12
Engineering Photosynthesis by Altering Cell Division Patterns
in the Leaf
Andrew Fleming
Department of Animal and Plant Sciences, University of Sheffield, United Kingdom
Abstract: The pattern of cell growth, division and separation during leaf development
determines the pattern and amount of airspace in a leaf. The resulting balance of cellular
material and airspace is expected to significantly influence the primary function of the leaf,
photosynthesis, yet the functional rules relating cell division pattern and separation, the
resultant airspace networks and photosynthetic performance remain largely unexplored at a
quantitative level. We have investigated the relationship of cell size and patterning, airspace
and photosynthesis by promoting and repressing the expression of cell cycle genes in the
leaf mesophyll. Using microCT imaging to quantify leaf cellular architecture and
fluorescence/gas exchange analysis to measure leaf function, we show that increased cell
density in the mesophyll of Arabidopsis can be used to increase leaf photosynthetic capacity.
Our analysis suggests this occurs both by increasing tissue density (decreasing the relative
amount of airspace) and by altering the pattern of airspace distribution within the leaf. A
deeper analysis of airspace networks sheds light on the trade-off between CO2 fixation and
water flux within the mesophyll and combining these approaches with computational
modelling of photosynthesis has allowed us to begin to identify structural parameters as
targets for improving leaf photosynthesis under future climate conditions. Overall, our
results indicate that cell division patterns influence the photosynthetic performance of a leaf
and that it is possible to engineer improved photosynthesis via this approach.
Key words: Photosynthesis, engineering, cell division, leaf
44
No.13
A Dynamic Model of Primary Metabolism in C3 Leaf
Honglong Zhao, Xinguang Zhu
Center of Excellence for Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences, 300 Feng Lin Road, Shanghai 200032, China
Abstract: The interactions among photosynthesis, photorespiration, dark respiration and
nitrogen assimilation in illuminated C3 leaf has been discussed for near 100 years. With the
global changing climate and increasing population size in recent years, it becomes increasing
popular as these interactions are especially important for metabolism design and engineering
during crops improvement. To systematically investigate the interplay of carbon and nitrogen
metabolism in C3 photosynthetic cells, we are developing a kinetic model which contains
the Calvin-Benson cycle (CBC), photorespiration (PR), gluconeogenesis-glycolysis (GL),
tricarboxylic acid cycle (TCA) and the nitrogen assimilation. Here, we are sharing the latest
progress of model construction. The primary metabolism model predicted the photosynthetic
rate, photorespiration flux, dark respiration and nitrogen assimilation rate in illuminated C3
leaf. The impacts of enzymatic shifting on metabolite concentration and metabolic fluxes in
silica were in consist with previous published data. These implied the potential application
of this model during photosynthesis and crop improvement by using synthetic biology in
future.
Key words: C3 photosynthesis, dynamic model, primary metabolism, metabolism
engineering, crop improvement
45
No.14
The Evolution of PPT1 and its Bidirectional Role in C4 Species
Ming-Ju Amy Lyu, Jianjun Jiang, Yaling Wang, Xinyu Liu, Xinguang Zhu
Center of Excellence for Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences, 300 Feng Lin Road, Shanghai 200032, China
Abstract: PPT translocates PEP between cytosol and plastid and provides PEP as the
precursor for shikimate pathway in plant species. Moreover, PPT is an integral part in CO2
concentration mechanism in C4 species. Two paralogs of PPT are reported in Arabidopsis,
namely, PPT1 and PPT2, which show different tissue preferential expression. In C3 species,
both PPT1 and PPT2 contribute to importing PEP into plastid for shikimate pathway in leaf
with PPT2 dominant in mesophyll cells and PPT1 only in bundle sheath cells. However, in
C4 species, PPT1 dominantly expressed in leaf especially in M cells, and PEP is required to
be exported to cytosol from chloroplast to capture CO2, which in contrast to the demand in
transporting direction in C3 species. Nowadays, it's not clear how PPT1 was recruited to C4
photosynthesis during the evolution and whether PPT1 in C4 remains the function of
importing PEP to chloroplast. This study combines evolutionary comparison and transgenic
experiment and illustrated that: (1) PPT1 was the ancestral paralog and PPT2 was derived
copy in the viridsplantae, (2) PPT1 was recruited to C4 photosynthesis at initial stage of the
evolution of C4 photosynthesis, besides PPT1 showed higher variance in both transcript
abundance and protein along the evolution of C4 photosynthesis than PPT2. (3) Transgenic
experiment suggested that the C4 PPT1 is a bidirectional transporter.
Key words: PPT1, evolution, C4 photosynthesis, bidirectional
46
No.15
CO2 Control System Based on an Optimized Regulation Model
Pingping Xin, Jin Hu, Haihui Zhang
Key Laboratory of Agricultural Internet of Things, Ministry of Agriculture; College of Mechanical and
Electronic Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China
Abstract: Existing carbon dioxide concentration control systems commonly use a
quantitative replenishment of CO2, without considering the effects of multiple environmental
factors on a plant’s photosynthetic rate and its characteristic impact on CO2 demand,
resulting in improper control of CO2 concentration. Accordingly, in this paper, a nested
combination experiment for the photosynthetic rate of cucumbers is presented. In order to
obtain a continuous carbon dioxide response curve, the photosynthetic rate prediction model
is established using the cucumber experimental data based on the support vector machine
algorithm. The network of the support vector machine photosynthetic rate prediction model
is used as an optimal objective function and the improved artificial fish swarm algorithm is
employed to search for the saturation of CO2 in a multidimensional nesting condition.
Further, the optimal CO2 regulation model based on multi-factor coupling is established
using the results of the above-mentioned experiments. Moreover, XOR verification of the
proposed model showed that the maximal relative error of the proposed optimal CO2
regulation model is 3.898%. Consequently, using a wireless sensor network platform, a
multi-sensor fusion-based CO2 control system is realized and verified. The verification
showed that the average relative error between the target CO2 value and the actual CO2 value
is 2.88%. At the same time, the average photosynthetic rateof the crop increased by 26.94%
compared to the contrasting region, which proves that the proposed system can achieve a
stable and reliable operation, greatly improving the efficiency of the environment of the
facility
Key words: Carbon dioxide control system, optimal CO2 regulation model, photosynthetic
rate, verification
47
No.16
Systematic Optimization of Whole Plant Carbon Nitrogen
Interaction (WACNI) to Support Crop Design for Greater Yield
Tiangen Chang, Xinguang Zhu
Center of Excellence for Molecular Plant Science, Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences, 300 Feng Lin Road, Shanghai 200032, China
Abstract: On the face of the rapid advances in genome editing technology and greatly
expanded knowledge on plant genome and genes, there is a strong demand to develop an
effective tool to guide designing crops for higher yields. Here we developed a highly
mechanistic model of Whole plAnt Carbon Nitrogen Interaction (WACNI), which predicts
crop yield based on major metabolic and biophysical processes in source, sink and transport
tissues. WACNI accurately predicted the yield responses of so far reported source, sink and
transport related genetic manipulations on rice grain yields. Systematic sensitivity analysis
with WACNI was used to classify the source, sink and transport related molecular processes
into four categories, i.e. universal yield enhancers, universal yield inhibitors, conditional
yield enhancers and weak yield regulators. Simulations using WACNI further show that even
without a major change in leaf photosynthetic properties, 54.6% to 73% grain yield increase
can be potentially achieved by optimizing these molecular processes during the rice grain
filling period while simply combining all the ‘superior’ molecular modules together cannot
achieve the optimal yield level. A common macroscopic feature in all these designed high-
yield lines is that they all show ‘a sustained and steady growth of grain sink’, which might
be used as a genetic selection criterion in high-yield rice breeding. Overall, WACNI can
serve as a tool to facilitate plant source sink interaction research and guide future crops
breeding by design.
Key words: Crop yields, grain filling, molecular breeding, source sink interaction
48
No.17
Identification and Expression of the Key Genes Involved in C4
Photosynthetic Pathway in Bread Wheat
Yingang Hu*, Daoura Gaoh Goudia Bachir, Yang Yang, Liang Chen
College of Agronomy, State Key Lab of Crop Stress Biology for Arid Areas, Northwest A&F
University, Yangling, Shaanxi, 712100, China; * Corresponding email: [email protected]
Abstract: Wheat is a C3 plant with relatively low photosynthetic efficiency, and with the
key genes involved in C4 photosynthetic pathway. Therefore, to understand the endogenous
expression patterns of C4 pathway genes in wheat, we identified the homologues of genes
encoding the key C4 pathway enzymes in bread wheat, then assessed their expression
patterns and enzymatic activities at three growth stages in flag leaves of 59 bread wheat
genotypes grown in 2014-2015 and 2015-2016 winter wheat growing seasons. Further, their
correlations with the photosynthetic rate, biomass and grain yield were investigated. The C4-
like genes homologous to PEPC, NADP-ME, MDH, and PPDK in maize were identified in
the A, B, and D sub-genomes of bread wheat, and located on the long arms of chromosomes
3 and 5 (TaPEPC), short arms of chromosomes 1 and 3 (TaNADP-ME), long arms of
chromosomes 1 and 7 (TaMDH), and long arms of chromosome 1 (TaPPDK), respectively.
All the four C4-like genes were expressed in the flag leaves at the three growth stages with
considerable variations among the 59 bread wheat genotypes. Significant differences were
observed on photosynthetic rates (A) of wheat genotypes with higher expressions of
TaPEPC_5, TaNADP-ME_1, and TaMDH_7 at heading and middle grain-filling stages and
those with intermediate and low expressions. Our results also indicated that the four C4
enzymes showed activities in the flag leaves and were obviously different among the 59
wheat genotypes. The activities of PEPcase and PPDK decreased at anthesis and slightly
increased at grain-filling stage, while NADP-ME and MDH exhibited a decreasing trend at
the three stages. The expressions of TaPEPC_5 and TaMDH_7 showed positive and
significant correlations with photosynthetic rate (A) and GYPP (grain yield plant-1) at
heading, anthesis and middle grain-filling stages. The activities of TaPEPC_5 (0.361) and
TaPPDK-1α (0.300) were positive and significantly correlated with GYPP only at middle
grain-filling, whereas the activities of TaMDH_7 displayed positive and significant
correlations with GYPP at heading (0.291) and middle grain-filling (0.300) stages. No
significant correlations were observed between the expressions and activities of the C4-like
enzymes with BMPP (biomass plant-1). Regression analysis revealed a weak linear
relationship (P<0.05) between above mentioned correlations.
Key words: Bread wheat, C4 photosynthetic pathway, gene expression, enzyme activity
49
No.18
Plasmodesmatal Flux in C3 and C4 monocots: The Metabolite
Pathway between Mesophyll and Bundle Sheath Cells
Florence Danila, Susanne von Caemmerer
ARC Centre of Excellence for Translational Photosynthesis, Australian National University, Canberra,
Australia
Abstract: Majority of the human population depend on rice for survival. Rice production
needs to increase by 50% to support food demand over the next 35 years. Traditional
breeding can only increase rice yield by 1% per annum. Switching the less efficient C3
photosynthetic system of rice (Oryza sativa) to use the more efficient C4 photosynthesis
would theoretically increase productivity by 50%. The aim of the C4 Rice Consortium is to
add features of C4 photosynthesis to the C3 plant, rice. Therefore, it is essential to know
whether rice can support the expected increase in metabolite flux between the leaf mesophyll
(M) and bundle sheath (BS) cells after all the C4 biochemistry has been installed. The main
pathway for metabolite flux is symplastic, i.e. via the plasmodesmata (PD) connecting M
and BS cells. Quantification of PD per cell interface area was done by combining electron
microscopy and 3D immunolocalisation. PD flux was calculated using photosynthetic
measurement, where CO2 assimilation rate was used as a surrogate for C4 acid fluxes. Results
revealed that C4 plants, setaria (Setaria viridis) and corn (Zea mays), had up to nine times
more PD per cell interface area than C3 plants, rice and wheat (Triticum aestivum). Estimates
of PD flux between M and BS cells using CO2 assimilation rates revealed flux rates of 2-3×
10-18 mol C4 acids s-1 per PD in C4 plants. These data on symplastic connections especially
between M and BS cells are essential for modelling studies and gene discovery strategies
needed to introduce aspects of C4 photosynthesis to C3 crops.
Key words: Plasmodesmatal flux, monocots, metabolite pathway, mesophyll cells, bundle
sheath cells
50
Poster Location and Hanging
The posters will be hung inside the Symposium room. The scaffold and pins
for the poster hanging will be provided. The poster number will be assigned for
each poster. Please hang your poster on your serial number (No.) location in
the day of your registration.
The Floor Plan for the Exhibitions
Each company exhibition platform has a dimension of 3.6 m × 2.0 m, with each provided
with two exhibition tables. The dimension of the table is 1.8 m × 0.6m. We will provide two
exhibition tables, two chairs and also one power outlet.
51
A Brief Introduction to Shanghai Institute of Plant
Physiology and Ecology (SIPPE), SIBS, CAS
Shanghai Institute of Plant Physiology and Ecology (SIPPE) is one of the institutions of
the Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Science (CAS).
SIPPE was established by the integration of the former Shanghai Institute of Plant
Physiology (SIPP) and Shanghai Institute of Entomology (SIE) on May 19th, 1999. The
former SIPP was initially evolved from the laboratory of Plant Physiology, Institute of
Botany of the Academia Sinica, which was founded at the town of Beipei, Chongqing on
May 1st, 1944. On January 23rd, 1953, Institute of Experimental Biology, CAS, was
separated from the Academia Sinica and became the predecessor of SIPP. It was the cradle
of plant physiology and biochemistry in China, and one of the pioneer institutions that
carried out molecular genetic researches in plants and microbes. Tremendous achievements
have been made in the fields of photosynthesis and nitrogen fixation in the early years.
Former SIE, one of the main research institutions on entomology in China, was founded in
1959 and once made great progresses in insect taxonomy, physiology, toxicology, co-
evolution, pesticide resistance and sex pheromones.
The mission of SIPPE is to generate knowledge of plants, microbes and insects through
creative research, to train scientists for the future, and to benefit the sustainable agriculture,
ecological environments, bio-energy and bio-manufacturing requirements in China. Our
research topics include, if not all, functional genomics and physiology, synthetic biology,
developmental and evolutionary biology, and biotechnologies by using plants, microbes and
insects as model organisms.
The research capacity of SIPPE has been continuously strengthened by the supports from
the funding agencies of the National Natural Science Foundation of China (NSFC) and the
Ministry of Science and Technology (MOST), Ministry of Agriculture and CAS. In
particular, three innovative research teams including “The system and synthetic biology
research of microbial metabolism” have been funded by NSFC, and three projects have won
52
the MOST's National Basic Research Program (“973”) and National Science and
Technology Infrastructure Program supports.
In 2017, a number of remarkable achievements were achieved, and more than 150 SCI
research papers were published, including Nature, Science, Nat Genetics, Energy &
Environment Science, Nature Communications, Molecular Cell, Cell Research, PNAS,
Developmental Cell, Plant Cell, PLoS Genetics and other important international academic
journals. In 2017, 38 Chinese invention patents, 6 PCT patents and 1 copyright in computer
software were applied; 24 Chinese invention patents and 4 PCT patents were authorized.
By the end of 2017, there are nearly 500 research scientists working at SIPPE including
more 96 professors, about 95 associate professors and senior technicians. SIPPE now has 9
academicians of Chinese Academy of Sciences, 1 academician of the American Academy
of Sciences, 4 academicians of The Academy of Sciences for the Developing World, 18
winners for the NSFC “National Outstanding Young Investigator Award”, 20 awardees for
“the One-Thousand-Talents” schemes, and over 35 awardees for the CAS “One-Hundred-
Talents” program. There are 573 graduate students (213 master students and 360 doctoral
students) and 104 postdoctoral researchers.
Up to now, it has established cooperative relations with many universities and research
institutions at home and abroad, such as the joint research center of plant and microbiological
sciences between Chinese academy of sciences and John Innes Centre, Huzhou industrial
biotechnology center of Shanghai institutes of biological sciences, Shanghai industrial
biotechnology center, Huzhou agricultural center, the Sibs-ETH research center of cassava
biotechnology, Sibs-Keygene joint laboratory of plant molecular breeding.
In the next five to ten years, SIPPE will continue to strengthen its research team by
recruiting outstanding young scientists, to improve its institutional managements, and to
improve its national and international competitiveness in plant, microbe and insect sciences.
The Institute will expand its collaborations with local and international institutions and
enterprises for related basic and translational research. In general, SIPPE will devote its
efforts to establish itself as a world-recognized institution by reinforcing its both basic and
applied research capacities.
53
A Brief Introduction to Laboratory of Photosynthesis
and Environmental Biology, SIPPE, SIBS, CAS
The Laboratory of Photosynthesis and Environmental Biology was formed in 2008 from
merging two laboratories, one for photosynthesis research and another for environmental
biology research. The former environmental biology originated in the afforestation in saline
soil in 1952 and cold resistance of rubber trees in 1955 guided by Dr. Zongluo Luo (Tsung-
lo Lo). The former photosynthesis laboratory was founded by Dr. Hongzhang Yin (Hung-
chang Yin) in 1956 as the first laboratory dedicated for photosynthesis research in China.
The former photosynthesis lab had made tremendous contribution to photosynthesis
research including mechanistic study of mechanisms underlying ATP synthesis, cyclic
electron transfer, factors controlling canopy photosynthesis, source sink interaction etc. The
formal laboratory of Environment has worked extensively on stress biology and space
biology. Currently, the Laboratory of Photosynthesis and Environmental Biology focuses
on understanding the molecular machineries of natural photosynthetic systems, with
particular emphasis on understanding processes or factors limiting photosynthetic energy
conversion efficiency in natural photosynthetic systems, and hence identifying novel
approaches to boost photosynthetic efficiency in crops for greater yields. The lab also
continues our long-held tradition of space biology and stress biology research. Working
together, we aim to be recognized as a lab which continuously delivers new theories and
approaches, including genes (either metabolic or regulatory genes) or pathways (both
natural and synthetic pathways) or new practices, to improve photosynthetic energy
conversion efficiency to benefit humanity.
Now, the Lab has 77 researchers including 2 academicians, 9 professors and 2 associate
professors, 6 Assistant professors, 50 graduate students, and 10 postdoctoral researchers.
The current research scientists (professors and associate professors) and their research areas
are listed below. Right now, we have positions at different levels open for scientists who
are dedicated to photosynthesis research for greater efficiency and greater yields.
54
The list of current research scientists and their research areas
Yun-Gang Shen Professor,
Academician
Photophosphorylation and regulation of
photosynthesis
Jiao-Nai Shi Professor,
Academician
Regulation of key enzymes in C4
photosynthetic carbon metabolism
Wei-Ming Cai Professor Stress biology, space biology
Gen-Yun Chen Professor photosynthetic energy conversion, regulation
of Rubisco
Hua-Ling Mi Professor
Regulation of photosynthetic electron
transports and the operation of photosynthetic
apparatus
Sheng Teng Professor Plant sugar signaling pathways, regulation of
photosynthesis carbon metabolism
Peng Wang Professor Genetic basis of Kranz anatomy, genetic
regulation of chloroplast ultrastructure
Hui-Qiong Zheng Professor Biology and biotechnology in microgravity,
hormone signaling and photosynthesis
Xin-Guang Zhu Professor,
Director
C4 rice engineering, photosynthesis systems
biology, ePlant
Ming-Nan Qu Associate
Professor
Natural variation of photosynthetic energy
conversion efficiency, Rubisco regulation,
stomata dynamics
Qing-Feng Song Associate
Professor
Canopy photosynthesis, photosystems antenna
size regulation
55
Participants' Information
Name Gender Institution Title Phone number E-mail
Andrea Bräutigam Female
Computational Biology, Faculty of Biology,
Bielefeld University, Bielefeld, 33615, Germany;
Institut für Biochemie der Pflanzen, Heinrich-Heine-
Universität Düsseldorf
Prof. andrea.braeutigam@uni-
duesseldorf.de
Asaph Cousins Male Washington State University, USA Prof. [email protected]
Ben Long Male
Plant Science Division, Research School of Biology,
College of Science, The Australian National
University, Canberra ACT 2601
Prof. +61 2 6125 2322 [email protected]
Chen Yang Female
CAS-Key Laboratory of Synthetic Biology, CAS
Center for Excellence in Molecular Plant Sciences,
Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences, Shanghai 200032,
China
Prof. [email protected]
Donald R. Ort Male
Departments of Plant Biology and Crop Sciences &
Carl R. Woese Institute for Genomic Biology,
University of Illinois, Urbana-Champaign, IL,
61801, USA
Prof. [email protected]
Fangfang Ma Female Shandong Agricultural University Prof. 86-18263803003 [email protected]
Kevin Griffin Male Columbia University, USA Prof. [email protected]
Maria Ermakova Famale
ARC Centre of Excellence for Translational
Photosynthesis, Australian National University,
Canberra, Australia
56
Name Gender Institution Title Phone number E-mail
Martin Parry Male Lancaster University Prof. +44 (0)1524 595084 [email protected]
Paul South Male USDA-ARS Photosynthesis Research Unit, 2Institute
for Genomic Biology, University of Illinois, USA Prof. [email protected]
Ron Milo Male Deptartment of Plant and Environmental Sciences,
Weizmann Institute of Science, Rehovot 76100, Israel Prof. 972-8-934-4466 [email protected]
Stephanie
Arrivault
Max Planck Institute of Molecular Plant Physiology,
Am Muehlenberg 1, 14476 Potsdam-Golm, Germany Prof. +493315678114 [email protected]
Susanne von
Caemmerer Female
ARC Centre of Excellence for Translational
Photosynthesis, Australian National University,
Canberra AUSTRALIA
Prof. +61-2-6125-5075 [email protected]
Tom Brutnell Male Danforth Plant Science Center Prof. [email protected]
Yi Xiao Male
Institute of Plant Physiology & Ecology, Shanghai
Institutes for Biological Sciences, Chinese Academy
of Sciences, Shanghai, China
Yi Yang Male
State Key Laboratory of Bioreactor Engineering, East
China University of Science and Technology,
Shanghai 200237, China
Prof. 86-21-64251311
(64251287) [email protected]
Yu Wang Female
Carl R. Woese Institute for Genomic Biology,
University of Illinois at Urbana-Champaign. 1206
West Gregory Drive, MC-195 Urbana, IL 61801 USA
57
Name Gender Institution Title Phone number E-mail
Florence Danila Female Australian National University Ms. +61 261254193 [email protected]
WeiYih Hee Male Australian National University Dr. +61420223289 [email protected]
Tiegang Lu Male Biotechnology Research Institute, Chinese Academy
of Agricultural Sciences Prof. 86-13671311011 [email protected]
Xiao Han Male Biotechnology Research Institute, Chinese Academy
of Agricultural Sciences Prof. 86-18210705601 [email protected]
Xiaofeng Gu Male Biotechnology Research Institute, Chinese Academy
of Agricultural Sciences Prof. 86-13717862966 [email protected]
Xuean Cui Male Biotechnology Research Institute, Chinese Academy
of Agricultural Sciences Mr. 86-18810442581 [email protected]
Muhammad Umair Male Center for Agricultural Resources Research, Institute
of Genetics and Developmental Biology of CAS Mr. 86-13126532700 [email protected]
Tabassum Hussain Male Center for Agricultural Resources Research, Institute
of Genetics and Developmental Biology of CAS Dr. 86-13068773020 [email protected]
Qiman Yunus Female College of Forestry and Horticulture, Xinjiang
Agricultural University Prof. 86-15199085223 [email protected]
Miao Ye Female College of Plant Science and Technology, Huazhong
Agricultural University Ms. 86-15623599396 [email protected]
Dongying Zhong Female College of Agronomy, Jiangxi Agricultural
University Ms. 86-15579771341 [email protected]
Jiahao Lu Male College of Agronomy, Jiangxi Agricultural
University Mr. 86-13585513816 [email protected]
Jiajia Han Female College of Agronomy, Jiangxi Agricultural
University Ms. 86-15797691593 [email protected]
58
Name Gender Institution Title Phone number E-mail
Jianfeng Cheng Male College of Agronomy, Jiangxi Agricultural
University Prof. 86-15900967950 [email protected]
Xiaoqiang Li Male College of Agronomy, Jiangxi Agricultural
University Mr. 86-18170879646 [email protected]
Yuliu Wang Male College of Agronomy, Jiangxi Agricultural
University Mr. 86-13918829945 [email protected]
Zejun Xiao Male College of Agronomy, Jiangxi Agricultural
University Mr. 86-18720772378 [email protected]
Aygul Abduwayit Male College of Forestry and Horticulture, Xinjiang
Agricultural University Prof. 86-13565876916 [email protected]
Xiong Zhuang Male Crop Physiology and Production Center, Huazhong
agricultural university Dr. 86-18354222331 [email protected]
Chuangjian Qian Male Heilongjiang Academy of Agricultural Sciences Dr. 86-13030048091 [email protected]
Tingting Du Female Huazhong Agricultural University Ms. 86-13545885635 [email protected]
Fei Zhou Female Huazhong Agricultural University Ms. 86-15207187316 [email protected]
Guanjun Huang Male Huazhong Agricultural University Mr. 86-17612726056 [email protected]
Huanying Li Female Huazhong Agricultural University Ms. 86-13163258269 [email protected]
Sicheng Liu Male Huazhong Agricultural University Mr. 86-13597444607 [email protected]
Taiyu Chen Male Huazhong Agricultural University Mr. 86-15972223893 [email protected]
Xiaoxiao Wang Female Huazhong Agricultural University Ms. 86-15271856514 [email protected]
Yuhan Yang Female Huazhong Agricultural University Ms. 86-17612763656 [email protected]
59
Name Gender Institution Title Phone number E-mail
Zhengcan Zhang Male Huazhong Agricultural University Mr. 86-15271820836 [email protected]
Jiang Zhang Male Hubei university Prof. 86-15827188121 [email protected]
Wenbo Xu Male Hubei university Mr. 86-18202725574 [email protected]
Bingran Zhao Male Hunan Hybrid Rice Research Center Prof. 86-13974804687 [email protected]
Pan Li Male Hunan Hybrid Rice Research Center Ms. 86-15974139909 [email protected]
Shuoqi Chang Male Hunan Hybrid Rice Research Center Dr. 86-13874878294 [email protected]
Xiabing Sheng Female Hunan Hybrid Rice Research Center Mr. 86-13107411851 [email protected]
Xiqin Fu Male Hunan Hybrid Rice Research Center Prof. 86-13507470768 [email protected]
Yaokui Li Male Hunan Hybrid Rice Research Center Mr. 86-13787310175 [email protected]
Youfa Liu Male Hunan Hybrid Rice Research Center Ms. 86-18670063763 [email protected]
Yuanyi Hu Female Hunan Hybrid Rice Research Center Dr. 86-18508467815 [email protected]
Huawei Li Female Iinstitute of Crop Research, Shandong Academy of
Agricultural Sciences Dr. 86-18006361505 [email protected]
Mei Li Female Institute of Biophysics, Chinese Academy of
Sciences Prof. 86-13671273472 [email protected]
Xue Ke Male
Institute of Biotechnology and Germplasm
Resources, Yunnan Academy of Agricultural
Sciences
Dr. 86-13529119587 [email protected]
Wenbin Zhou Male Institute of Crop Sciences, Chinese Academy of
Agricultural Sciences, Dr. 86-13264097381 [email protected]
Lei Zhang Male Institute of Genetics and Developmental Biology,
Chinese Academy of Sciences Dr. 86-18510238204 [email protected]
60
Name Gender Institution Title Phone number E-mail
Lijiao Zhang Female Institute of Genetics and Developmental Biology,
Chinese Academy of Sciences Dr. 86-15201130581 [email protected]
Yansheng Wu Male Institute of Geochemistry, Chinese Academy of
Sciences Dr. 86-17625901936 [email protected]
Xia Li Female Jiangsu Academy of Agricultural Sciences Prof. 86-13062503186 [email protected]
Li Liu Female Kuming Institue of Botany, Chinese Academy of
Sciences Prof. 86-15398712027 [email protected]
Lixia Zhang Female Liaoning Academy of Agricultural Sciences Ms. 86-15804090353 [email protected]
Miao Ye Female College of Plant Science and Technology, Huazhong
Agricultural University Ms. 86-15623599396 [email protected]
Feng Xiao Male Nanjing Agricultural University Mr. 86-15295595319 [email protected]
Hongfa Xu Male Nanjing Agricultural University Ms. 86-15295570236 [email protected]
Shiwei Guo Male Nanjing Agricultural University Prof. 86-13770827567 [email protected]
Wei Lu Male Nanjing Agricultural University Dr. 86-13951765719 [email protected]
Yonghui Pan Male Nanjing Agricultural University Mr. 86-13611502578 [email protected]
Zhifeng Lu Male Nanjing Agricultural University Dr. 86-15872373191 [email protected]
Zongfeng Yang Male Nanjing Agricultural University Ms. 86-18852052199 [email protected]
Kailiu Xie Female Nanjing Agricultural University Ms. 86-15105195661 [email protected]
Limin Gao Female Nanjing Agricultural University Dr. 86-15651703212 [email protected]
Ji Li Female Nanjing University Ms. 86-15189828680 [email protected]
Yue Wang Female Northeast Forestry University Dr. 86-15546447570 [email protected]
61
Name Gender Institution Title Phone number E-mail
Chunmei Gong Female Northwest Agriculture & Forestry University Prof. 86-13669209673 [email protected]
Latai Zhu Female Northwest Agriculture & Forestry University Ms. 86-18215100250 [email protected]
Pingping Xin Male Northwest Agriculture & Forestry University Ms. 86-18729860087 [email protected]
Wenfei Zhou Female Northwest Agriculture & Forestry University Ms. 86-18792896644 [email protected]
Yang Yang Male Northwest Agriculture & Forestry University Mr. 86-15291818271 [email protected]
Yingang Hu Male Northwest Agriculture & Forestry University Prof. 86-13572570219 [email protected]
Xiaonan Zang Female Ocean University of China Prof. 86-13969816228 [email protected]
Xuecheng Zhang Male Ocean University of China Prof. 86-13953266901 [email protected]
Chen Kuang Male Oil Crops Research Institute of the Chinese
Academy of Agricultural Sciences Mr. 86-13260677689 [email protected]
Jun Liu Male Oil Crops Research Institute of the Chinese
Academy of Agricultural Sciences Dr. 86-15607115298 [email protected]
Wei Hua Female Oil Crops Research Institute of the Chinese
Academy of Agricultural Sciences Prof. 86-13886138046 [email protected]
Xiaoyi Zhu Male Oil Crops Research Institute of the Chinese
Academy of Agricultural Sciences Dr. 86-13554623134 [email protected]
Li Wei Male Qingdao Institute of Bioenergy and Bioprocess
Technology, Chinese Academy of Sciences Dr. 86-13730908512 [email protected]
Yangen Fan Male Shandong Agricultural University Dr. 86-17662356880 [email protected]
Ying Liang Female Shandong Agricultural University Ms. 86-18854857253 [email protected]
Yuenan Li Female Shandong Agricultural University Ms. 86-18206385908 [email protected]
Yuting Li Male Shandong Agricultural University Dr. 86-18254887836 [email protected]
Zishan Zhang Male Shandong Agricultural University Dr. 86-13581191679 [email protected]
62
Name Gender Institution Title Phone number E-mail
Honglong Zhao Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-18939910730 [email protected]
Jemaa Essemine Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Dr. 86-18516130902 [email protected]
Duanfeng Xin Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-15026549652 [email protected]
Faming Chen Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-18922748793 [email protected]
Fenfen Miao Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-17621862830 [email protected]
Genyun Chen Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Prof. 86-18016495953 [email protected]
Huixian Zhu Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-17750219847 [email protected]
Mengyao Wang Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-15800973163 [email protected]
Ming-Ju Amy Lyu Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-15221358785 [email protected]
Mingnan Qu Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Dr. 86-13022108559 [email protected]
Naveed Khan Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-18521737491 [email protected]
Qiming Tang Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-18521727793 [email protected]
63
Name Gender Institution Title Phone number E-mail
Qingfeng Song Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Dr. 86-13564082434 [email protected]
Shahnaz Perveen Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-18512101092 [email protected]
Tiangen Chang Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Dr. 86-13917166684 [email protected]
Xinguang Zhu Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Prof. 86-13917058786 [email protected]
Xinyu Liu Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-18616852011 [email protected]
Yanjie Wang Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-15601753715 [email protected]
Yongyao Zhao Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-18717873105 [email protected]
Yuhui Huang Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-18780058907 [email protected]
Zai Shi Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-15105419285 [email protected]
Zhan Shu Female Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Ms. 86-13761227208 [email protected]
Zhiwei Zhou Male Shanghai Institute of Plant Physiology and Ecology,
Chinese Academy of Sciences Mr. 86-17621779660 [email protected]
64
Name Gender Institution Title Phone number E-mail
Bender Leslie
Michael Male Shanghai Jiaotong University Prof. +1 6092582936 [email protected]
Yali Zhang Male Shihezi University Dr. 86-18999530985 [email protected]
Zhibo Li Male Shihezi University Dr. 86-189099363335 [email protected]
Chanjuan Ye Female South China Agricultural University Ms. 86-13480295738 [email protected]
Erdong Ni Male South China Agricultural University Mr. 86-15818154046 [email protected]
Ruiqi Li Female South China Agricultural University Ms. 86-13610133583 [email protected]
Ying He Female South China Agricultural University Ms. 86-15876596509 [email protected]
Hesheng Yao Male Southwest University Dr. 86-15001636930 [email protected]
Andrew Fleming Male University of Sheffield Prof. 0114 2224830 [email protected]
Feifei Zhang Female Xishuangbanna Tropical Botanical Garden, Chinese
Academy of Sciences Ms. 86-13108802883 [email protected]
Dongsheng An Male Zhanjiang Experimental Station of Chinese
Academy of Tropical Agricultural Science Mr. 86-15219586106 [email protected]
Chunfang Zheng Female Zhejiang Mariculture Research Institute Prof. 86-15058926073 [email protected]
65
Notes of Symposium
66
Notes of Symposium
67
Notes of Symposium
68
Notes of Symposium
69
Notes of Symposium
70
Notes of Symposium
71
Notes of Symposium
72
Notes of Symposium
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Accommodation & Transportation
The symposium will be held in Lake Malaren International Convention Center, which
locate only 600 meters away from “Meilan Lake Station” of Metro Line 7, about 10 minutes’
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