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Bismillahirrohmaanirrohiim
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Introduction
A. Background
The increasing of Diabetes Mellitus incidence
Diabetes Mellitus can cause dislipidemia
(hypertriglycerides)
Aloe vera as a herbal therapy
B. Problem Formulation
DoesAloe verajuice can reduce blood triglycerideslevel inRattus norvegicus induced by alloxan?
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C. Originality of The Study
Perdana. D (1995) research about Allium sativum L. Oil and
Aloe vera L. Gel Imunomodulator Activity Test
Mamata Chandrakar, et al (2008) research about
Hypocholesterolemic Effect ofAloe vera L. Extract on High
Cholesterol Fed Calotes versicolorDaudin
D. Objectives of The Study
To decrease the incidence of Diabetes Mellitus
To know the effect ofAloe verajuice for reducing blood Tglevel ofRattus norvegicus induced by alloxan
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E. Significance of The Study
This research can give the information about herbal
therapy to other people and also be a good source for otheranalyst to make the other research especially about Aloe vera,
Diabetes Mellitus, and blood triglycerides.
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Literature Review
A. Literature Aloe vera and Its subtances
Diabetes Mellitus
a. IDDM
b. NIDDM
Triglyceride & Cholesterol metabolism
Diabetes Mellitus increase blood triglyceride level
Alloxan for inducing Diabetes Mellitus
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B. Conceptional Framework
Herbal Therapy
Aloe veraJuice
Therapy for DM :
Education
Diet
Exercise
Pharmacology Therapy
Diabetes Mellitus
Insulin Resistance
Increasing Blood
Triglyceride
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C. Hypothesis
Aloe verajuice can reduce the blood triglycerides level in
Rattus norvegicus induced by alloxan.
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Research Methode
A. Research DesignIt was an experimental research with pre and post test control
design.
B. Research SettingResearch was conducted in laboratory of Medical Faculty in
Yogyakarta Muhammadiyah University within two months.
c. Research SubjectThis research used twelve Rattus norvegicus from Sprague
dawley with three months age and 150-250 gr weight.
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D. Inclusion and Exclusion Criteria
1. Inclusion :
a. TwentyRattus norvegicus fromsprague dawley
b. Female
c. Three months age
d. 150-250 gr for weight
2. Exclusion :
Rattus norvegicus gets sick before research or control.
E. Research Variables Independent variable :Aloe vera
Dependent variable : Blood triglycerides level in eachsubject
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F. Operational Definition
Diabetes Mellitus
Dislipidemia (hypertriglycerides)
Aloe vera
Alloxan
G. Research Instruments and Materials
Materials :
a. A lo e vera juice
b . A llo xan
c. Blood ofRattus norvegicu sd. Aquadest
Instruments :
a. Rat cage
b. Weight scale
c. Oral sonded. Blender
e. Pan
f. Pipette
g. Filter
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H. Research Implementation Phases
Subject
Checking blood glucose & triglycerides
Give the al loxan150 mg/kgbb
subkutan
Checking blood glucose & Tg after 48 hours
Negative control
(A)Group B Group C Group D
Give nothing5mg/kgbb/day
Aloe vera
juice
5.5mg/kgbb/day
Aloe vera
juice
6 mg/kgbb/day
Aloe verajuice
Checking blood triglycerides after giving the Aloe
verajuice for 14 days
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I. Data Analysis
This research usedPaired-t-testin each groups before and
after treatment (gave Aloe vera juice). Blood triglycerides
from group A, B, C and D were analysed using ANOVA test.
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Mean of Blood Tg Level Before and After Induced by alloxan
Result of Analysis
No Groups
Mean of TrigliseridS.D
Before After
1 Group A (negative control) 103,194,44 110,182,75
2 Group B (Aloe verajuice 5 mg/kgBB) 101,412,71 109,374,11
3 Group C (Aloe vera juice 5,5 mg/kgBB) 102,154,41 111,074,05
4 Group D (Aloe vera juice 6 mg/kgBB) 101,414,02 113,603,76
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Mean of Blood Tg Level Before and After Treatment in Each Groups
No Groups
Mean of TrigliseridS.D
Before After
1 Group A (negative control) 110,182,75 115,883,31
2 Group B (Aloe verajuice 5 mg/kgBB) 109,374,11 104,412,84
3 Group C (Aloe vera juice 5,5 mg/kgBB) 111,074,05 92,352,11
4 Group D (Aloe vera juice 6 mg/kgBB) 113,603,76 78,122,19
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The result ofpaired t testshowed there was a
significant decrease in statistical (p
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Conclusion
This research showed that giving an Aloe vera juicewith 5, 5,5 and 6 mg/kgBB/day in 14 days can decrease the
blood triglycerides level in Rattus norvegicus induced by
alloxan.
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Suggestion
For upcoming research, it is necessary to do thisexperimental research with more samples and more time
periods.
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References
WHO. (2009). Diabetes ; Country and Regional Data
ADA. (2009). All About Diabetes
HIMAPID, (2008) ,Diabetes Militus, Universitas
Hasanudin, Sulawesi selatan
Beckman et al., (2002). Patient with Diabetik. DiabetikJournal,1-8.
Jakus, (2000), Diabetes Militus, Jakarta.
Sudoyo W.Aru, Setiyohadi B, dkk. Ilmu Penyakit
Dalam jilid III.Jakarta : Departemen Ilmu PenyakitDalam FKUI
Wijaya, Hembing. 2000. Potensi Tumbuhan Obat Asli
Indonesia Sebagai Produk Kesehatan, Risalah
Pertemuan Ilmiah Penelilian dan Pengembangan
Teknologi Isolop dan Radiasi, Jakarta.
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Santoso, Budi. 2008. Ragam dan Khasiat Tanaman
Obat. Jakarta : Agromedia Pustaka.
Chandrakar Mamata, Palekar Sachin, Chirade Sudhir,
& Hafiz M.A. (2008). Hypocholesterolemic Effect of
Aloe vera (L.) Extract on High Cholesterol Fed
Calotes versicolorDaudin.Asian Journal
Experimental Sciences, 22, 295-298.
Yagi Akira, Hegazy Sahar, Kabbash Amal, & WahabEngy Abd-El. (2009). Possible Hypoglycemic Effect of
Aloe vera L. High Molecular Weight Fractions On
Type 2 Diabetic Patients. Saudi Pharmaceutical
Journal, 17, 210-217. Eriko Misawa, Miyuki Tanaka, Kouji Nomaguchi, &
Muneo Yamada. (2008). Administration of
phytosterols isolated from Aloe vera gel reduce
visceral fat mass and improve hyperglycemia inZucker diabetic fatt ZDF rats.Asian Oceanian
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Thank You
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