0

10

20

30

40

50

60

0 5 10 15Dex [nM]

% S

ub-G

1 P

D1.

6 C

ells

0µM GSNO

50µM GSNO

75µM GSNO

100µM GSNO

Suppl. Fig. 1

y = 1.01x + 3.34

y = 2.33x + 7.12

y = 3

.20x

+ 1

3.57y

= 5.

03x

+ 17

.51

Figure Legend. NO· synergizes with GC in inducing apoptosis of PD1.6 cells. PD1.6 were incubated o.n. with various concentrations of Dexamethasone (Dex) and/or S-Nitrosoglutathione (GSNO). The extent of apoptosis was measured by cell cycle analysis. A representative of eight separate experiments is shown. Statistical bars shown in the graphs correspond to mean +/- SD. The equation of the linear curve between 1-5nM Dex was calculated. The differences in the slopes indicate a synergistic effect.