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Super-Resolution Imaging System
NanoBioImaging Ltd.
Three-Dimensional Simultaneous Multi-Channel Super Resolution Imaging
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Powerful Tool of ScientistsSuper-resolution microscopy has become a powerful imaging tool for biology and material science. To obtain reliable and high-resolution imaging results, it requires not only the knowledge of biology, but also the knowledge of physics, chemistry, computer science, and engineering. Yet, it has been challenging for most scientists.
In collaboration with HKUST Super-Resolution Imaging Center, NanoBioImaging (NBI) has built an inter-disciplined expert team covering optics, computer science, biology and chemistry. NBI offers customized products to our clients and comprehensive support including hardware maintenance, software update, protocol design, data analysis, and on-site and on-line troubleshooting services in all aspects.
Stable and efficient hardware Professional analysis software
User-friendly software
Comprehensive technical support
Easy and seamless workflow Running in general lab conditions, even at anexhibition
Advantages
Your Best Choice
20 nm resolution 10 times greater than conventional optical microscopes
Simultaneous multi-channel imaging Time-efficient, minimizing photobleaching
Active locking system with nanometer precision Highly stable and efficient
Optimized imaging buffer
No warm-up time required
Balanced photochemical properties for different channels
Time-saving
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Stable and Efficient Hardware
NBI developed active locking system to correct the sample drifting in real-time during data acquisition. The samples can be “locked” with nanometer precision and no post-acquisition correction is required. This not only guarantees reliable results without artifacts caused by software correction, but also saves users' time waiting for system stabilization and thermal equilibrium after each sample loading.
NBI featured simultaneous multi-channel imaging delivers super resolution images of multiple targets at the same time, shortening the data acquisition time to 1/3 of other commercial products. What's remarkable, it minimizes photobleaching which is severe in sequential super-resolution imaging of multiple channels.
Most stable and reliable
Highly efficient
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Rohdea is a intelligent and user-friendly super-resolution imaging software developed by NBI. It produces reliable data and provides users with the best operation experience.
Intelligent and User-friendly Software
1、Simplified imaging process
"What You See Is What You Get", simplifies operation process, and is user-friendly.We summarize the imaging process into four steps of the super-resolution imaging: "choosing region"-"widefield capture"-"parameter adjustment"-"super-resolution capture".
2、Real-time and transparent parameter adjustment
The imaging results are subject to the parameters used in the data acquisition process. Rohdea provides a real-time parameter adjustment module that allows users to visually compare the effects of parameter changes on imaging results.
3、Up-to-date algorithms and real-time data reconstruction
Rohdea integrates multiple up-to-date algorithms and functions for data acquisition and data processing. In particular, the results and parameters of SR image reconstruction are presented in real time, which is convenient for users to adjust in time.
4、Convenient user data management
Rohdea allows the generation, modification, and automatic saving of parameter settings and imaging results. Besides, it provides parameter template for similar experiments, which is convenient for users to select and optimize in subsequent experiments.
5、Strong data analysis features
Rohdea implements algorithms including clustering analysis, co-localization analysis, distance measurement, image processing, etc., delivering results that can be directly used for publications.
Simplified imaging process
Real-time and transparent parameter adjustment
Convenient user data
management
Up-to-date algorithms and real-time data reconstruction
Strong data analysis features
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Full Service
Professional training-more than operating the instrument
Experts from NBI team cover physics computer science, biology and chemistry. NBI is ready to share our knowledge of principles, mechanisms and practices in all aspects of super-resolution microscopy with our customers.
Comprehensive support
Super resolution imaging solution
With our varied technical background and extensive imaging experiences, NBI experts help users’ imaging kick off right away by providing project consultation, feasibility analysis, protocol design etc..
Sharing our knowledge base of sample preparation and imaging solutions
We share our optimized sample preparation protocols and imaging configuration templates with our users, with the aim of helping they obtain the best imaging results within the shortest time.
Online and on-site support beyond maintenance
Our technical support not only includes hardware maintenance, but also on-line and on-site consultation on rational of super-resolution fluorescent imaging, experimental protocols, data analysis and modeling, manuscript drafting, etc..
Long-term collaboration-growing up together with users
NBI is willing to collaborate with customers in a long-term manner. We will provide customers our latest techniques. We are also willing to meet your individualized technical demand.
Imaging service
Results delivered within one week
Clients NBI
Consultation and sample delivery
Best images
and reports
For free trails, please contact [email protected]
mitochondrial inner and outer membranes
synapse
1 μm200 nm
200 nm
(Images acquired with NBI SRiS 2.0, in cooperation with Herrup Group in HKUST)
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Examples of Application
Fields of application
Cell biology
Subcellular structures
Neuroscience
Protein localization
Microbiology Immunology
Protein-protein interaction
Nanomaterials …
Chromatin organization …
Figure 3. Three-channel super-resolution images of COS7 cel l organel les. (Fig. a) Mitochondrial outer membrane protein TOM20 (magenta), mitochondrial matrix protein COX IV (blue), and Golgi protein TGN46 (green). (Fig. b) Mitochondrial outer membrane TOM20 (red), nuclear membrane protein lamin (blue), and Golgi protein TGN46 (green).
(Images acquired with NBI SRiS 3.0 by NBI)
Organelles
Figure 1. Super-resolution imaging of neuron synapse in mouse brain slices. (Fig. a, c & e) Widefield images of the synapse structures. (Fig. b, d & f) Super-resolution images showing the synapse and well-separated pre-synapse & post-synapse structures.
( Images acquired wi th NBI SRiS 2.0, in cooperation with Herrup group in Hong Kong University of Science and Technology)
Neuron Synapse
Widefield Super-resolution
1 μm 1 μm200 nm
200 nm 200 nm
200 nm
Figure 2. Three-dimentional super-resolution images of telomere DNA. (Fig. a) Widefield image of single focus formed by telomere DNA. (Fig. b & c) Super-resolution images of two telomere foci, corresponding to the foci within the white boxes in the widefield image. Super resolution images show detailed structures, of which the changes within each focus could be easily identified.
( Images acqu i red w i th NBI SRiS 2 .0 , in cooperation with Yong Zhao group from Sun Yat-sen University)
Nucleic acid-FISH
Widefield Super-resolution
(a) (b)
(c)
1 μm
1 μm
(a) (b)
1 μm 1 μm
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Examples Of Application
Golgi Apparatus
Figure 4. Super-resolution images of cis-Golgi network
protein GM 130 and trans-Golgi network protein Golgin
97. (a) Widefield image shows the overlapping of these two
proteins. (b) Super-resolution image clearly shows that the
two proteins locate at two layers of golgi apparatus.
(Images acquired with NBI SRiS 2.0, in cooperation with Guo
Lab in Hong Kong University of Science and Technology)
Yeast Organelle
Figure 5. Super-resolution images of tubulin proteins
Tub1 and Tub4 in yeast spindle-pole body. GFP and RFP
tagged tubulin proteins were transfected into the yeast
cell, and further stained with GFP nanobody and RFP
nanobody, respectively. (Fig. a & b) widefield images. (Fig.
c & d) Corresponding super-resolution images, two-layer
structure of γ-tubulin complex was resolved.
(Images acquired with NBI SRiS 2.0, in cooperation with
Dr Yuanliang Zhai in Hong Kong University of Science and
Technology)
Mitochondria
Figure 6. 3D Three-dimensional super-resolut ion
images of mitochondria in COS7 cell. The mitochondrial
outer membrane TOM20 was labelled indirectly. The
mitochondria showed hollow tube structure.
(Images acquired with NBI SRiS 2.0 by NBI)
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Selected Publications by NBI SRiS Users
A. Cheng, T. Zhao, KH. Tse, HM. Chow, Y. Cui, L. Jiang, S. Du, MMT. Loy, K. Herrup, "ATM and ATR play
complementary roles in the behavior of excitatory and inhibitory vesicle populations." Proc Natl Acad Sci U S A.
2018 Jan 9;115(2):E292-E301.
H. Huang, X. Lin, Z. Liang, T. Zhao, S. Du, M.M.T. Loy, K.-O. Lai, A.K.Y. Fu, & N. Ip, “Cdk5-mediated
phosphorylation of liprina1 is critical for neuronal activity-dependent synapse development,”Proceedings of the
National Academy of Sciences. 2017 Aug 15;114(33):E6992-E7001.
Au Franco K.C., Jia Y., Jiang K. , Grigoriev I., Hau Bill K.T. , Shen Y. H. , Du S.W. , Akhmanova A., Qi Robert Z..”
GAS2L1 Is a Centriole-Associated Protein Required for Centrosome Dynamics and Disjunction.” Developmental
cell. 2017 Jan 9;40(1):81-94.
X. Gu, E. Zhao, T. Zhao, J.W.Y. Lam, S. Du, M.M.T. Loy, & B.Z. Tang, “A mitochondrion-specific photoactivatable
turn-on bioprobe for super-resolution imaging under physiological conditions without additives, “ Advanced
Materials, 2016, 28 (25), 5064-5071.
H. Wang, X. Zhuang, X. Wang, A.H.Y. Law, T. Zhao, S. Du, M.M.T. Loy, & L. Jiang, “A Distinct Pathway for Polar
Exocytosis in Plant Cell Wall Formation.” Plant Physiol. 2016 Oct;172(2):1003-1018.
G. Vail, A. Cheng, Y.R. Han, T. Zhao, S. Du, M.M.T. Loy, K. Herrup, & M. R. Plummer, “ATM protein is
located on presynaptic vesicles and its deficit leads to failures in synaptic plasticity," J Neurophysiology, 2016,
116 (1), 201-209.
Y. Du, H. Zhang, M. Zhao, D. Zou, and C. J. Xue, “Faster super-resolution imaging of high density molecules via
a cascading algorithm based on compressed sensing,” Opt. Express 23, 18563 (2015).
T. Zhao, Y. Wang, Y. Zhai, X. Qu, A. Cheng, S. Du, & M.M.T. Loy, “A user-friendly two-color super-resolution
localization microscope,” Opt. Express 23, 1879 (2015).
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System Configuration
SRiS 2.0 Series
SRiS 3.0 Series
Two-Channel Standard Two-Channel Advanced Two-Channel Professional
sCMOS detector sCMOS detector sCMOS detector
Microscope Microscope Microscope
Nano XYZ stage (Manual) Nano XYZ stage (Auto) Nano XYZ stage (Auto)
Opto-split ii Opto-split ii Opto-split ii
Optical element Optical element Optical element
656 and 750 nm lasers 656 and 750 nm lasers 656 and 750 nm lasers (Advanced)
Channel filter Channel filter Channel filter
Workstation Workstation Workstation
Optical table Optical table Optical table
NBI Active Locking System element NBI Active Locking System element NBI Active Locking System element
NBI outlook NBI outlook NBI outlook
NBI automatic control system NBI automatic control system NBI automatic control system
NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Advanced)
Three-Channel Standard Three-Channel Advanced Three-Channel Professional
sCMOS detector sCMOS detector sCMOS detector
Microscope Microscope Microscope
Nano XYZ stage (Manual) Nano XYZ stage (Auto) Nano XYZ stage (Auto)
Opto-split iii Opto-split iii Opto-split iii
Optical element Optical element Optical element
561, 656, and 750 nm lasers 561, 656, and 750 nm lasers 561, 656, and 750 nm lasers
Channel filter Channel filter Channel filter
Workstation Workstation Workstation
Optical table Optical table Optical table
NBI Active Locking System element NBI Active Locking System element NBI Active Locking System element
NBI outlook NBI outlook NBI outlook
NBI automatic control system NBI automatic control system NBI automatic control system
NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Advanced)
SEEINGIS
BELIEVING
Web
www.nbi.hk
Address
Rm 505, Biotech Centre 1, No.9 Science Park West Avenue, Hong Kong Science Park, Shatin, N.T., Hong Kong
Tel
(852) 2688 0511
(86) 574 6302 8365
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