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Super-Resolution Imaging System

NanoBioImaging Ltd.

Three-Dimensional Simultaneous Multi-Channel Super Resolution Imaging

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Powerful Tool of ScientistsSuper-resolution microscopy has become a powerful imaging tool for biology and material science. To obtain reliable and high-resolution imaging results, it requires not only the knowledge of biology, but also the knowledge of physics, chemistry, computer science, and engineering. Yet, it has been challenging for most scientists.

In collaboration with HKUST Super-Resolution Imaging Center, NanoBioImaging (NBI) has built an inter-disciplined expert team covering optics, computer science, biology and chemistry. NBI offers customized products to our clients and comprehensive support including hardware maintenance, software update, protocol design, data analysis, and on-site and on-line troubleshooting services in all aspects.

Stable and efficient hardware Professional analysis software

User-friendly software

Comprehensive technical support

Easy and seamless workflow Running in general lab conditions, even at anexhibition

Advantages

Your Best Choice

20 nm resolution 10 times greater than conventional optical microscopes

Simultaneous multi-channel imaging Time-efficient, minimizing photobleaching

Active locking system with nanometer precision Highly stable and efficient

Optimized imaging buffer

No warm-up time required

Balanced photochemical properties for different channels

Time-saving

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Stable and Efficient Hardware

NBI developed active locking system to correct the sample drifting in real-time during data acquisition. The samples can be “locked” with nanometer precision and no post-acquisition correction is required. This not only guarantees reliable results without artifacts caused by software correction, but also saves users' time waiting for system stabilization and thermal equilibrium after each sample loading.

NBI featured simultaneous multi-channel imaging delivers super resolution images of multiple targets at the same time, shortening the data acquisition time to 1/3 of other commercial products. What's remarkable, it minimizes photobleaching which is severe in sequential super-resolution imaging of multiple channels.

Most stable and reliable

Highly efficient

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Rohdea is a intelligent and user-friendly super-resolution imaging software developed by NBI. It produces reliable data and provides users with the best operation experience.

Intelligent and User-friendly Software

1、Simplified imaging process

"What You See Is What You Get", simplifies operation process, and is user-friendly.We summarize the imaging process into four steps of the super-resolution imaging: "choosing region"-"widefield capture"-"parameter adjustment"-"super-resolution capture".

2、Real-time and transparent parameter adjustment

The imaging results are subject to the parameters used in the data acquisition process. Rohdea provides a real-time parameter adjustment module that allows users to visually compare the effects of parameter changes on imaging results.

3、Up-to-date algorithms and real-time data reconstruction

Rohdea integrates multiple up-to-date algorithms and functions for data acquisition and data processing. In particular, the results and parameters of SR image reconstruction are presented in real time, which is convenient for users to adjust in time.

4、Convenient user data management

Rohdea allows the generation, modification, and automatic saving of parameter settings and imaging results. Besides, it provides parameter template for similar experiments, which is convenient for users to select and optimize in subsequent experiments.

5、Strong data analysis features

Rohdea implements algorithms including clustering analysis, co-localization analysis, distance measurement, image processing, etc., delivering results that can be directly used for publications.

Simplified imaging process

Real-time and transparent parameter adjustment

Convenient user data

management

Up-to-date algorithms and real-time data reconstruction

Strong data analysis features

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Full Service

Professional training-more than operating the instrument

Experts from NBI team cover physics computer science, biology and chemistry. NBI is ready to share our knowledge of principles, mechanisms and practices in all aspects of super-resolution microscopy with our customers.

Comprehensive support

　　Super resolution imaging solution

With our varied technical background and extensive imaging experiences, NBI experts help users’ imaging kick off right away by providing project consultation, feasibility analysis, protocol design etc..

　　Sharing our knowledge base of sample preparation and imaging solutions

We share our optimized sample preparation protocols and imaging configuration templates with our users, with the aim of helping they obtain the best imaging results within the shortest time.

　　 Online and on-site support beyond maintenance

Our technical support not only includes hardware maintenance, but also on-line and on-site consultation on rational of super-resolution fluorescent imaging, experimental protocols, data analysis and modeling, manuscript drafting, etc..

　　 Long-term collaboration-growing up together with users

NBI is willing to collaborate with customers in a long-term manner. We will provide customers our latest techniques. We are also willing to meet your individualized technical demand.

　　 Imaging service

Results delivered within one week

Clients NBI

Consultation and sample delivery

Best images

and reports

For free trails, please contact imagingservice@nbi.hk

mitochondrial inner and outer membranes

synapse

1 μm200 nm

200 nm

(Images acquired with NBI SRiS 2.0, in cooperation with Herrup Group in HKUST)

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Examples of Application

Fields of application

Cell biology

Subcellular structures

Neuroscience

Protein localization

Microbiology Immunology

Protein-protein interaction

Nanomaterials …

Chromatin organization …

Figure 3. Three-channel super-resolution images of COS7 cel l organel les. (Fig. a) Mitochondrial outer membrane protein TOM20 (magenta), mitochondrial matrix protein COX IV (blue), and Golgi protein TGN46 (green). (Fig. b) Mitochondrial outer membrane TOM20 (red), nuclear membrane protein lamin (blue), and Golgi protein TGN46 (green).

(Images acquired with NBI SRiS 3.0 by NBI)

Organelles

Figure 1. Super-resolution imaging of neuron synapse in mouse brain slices. (Fig. a, c & e) Widefield images of the synapse structures. (Fig. b, d & f) Super-resolution images showing the synapse and well-separated pre-synapse & post-synapse structures.

( Images acquired wi th NBI SRiS 2.0, in cooperation with Herrup group in Hong Kong University of Science and Technology)

Neuron Synapse

Widefield Super-resolution

1 μm 1 μm200 nm

200 nm 200 nm

200 nm

Figure 2. Three-dimentional super-resolution images of telomere DNA. (Fig. a) Widefield image of single focus formed by telomere DNA. (Fig. b & c) Super-resolution images of two telomere foci, corresponding to the foci within the white boxes in the widefield image. Super resolution images show detailed structures, of which the changes within each focus could be easily identified.

( Images acqu i red w i th NBI SRiS 2 .0 , in cooperation with Yong Zhao group from Sun Yat-sen University)

Nucleic acid-FISH

Widefield Super-resolution

(a) (b)

(c)

1 μm

1 μm

(a) (b)

1 μm 1 μm

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Examples Of Application

Golgi Apparatus

Figure 4. Super-resolution images of cis-Golgi network

protein GM 130 and trans-Golgi network protein Golgin

97. (a) Widefield image shows the overlapping of these two

proteins. (b) Super-resolution image clearly shows that the

two proteins locate at two layers of golgi apparatus.

(Images acquired with NBI SRiS 2.0, in cooperation with Guo

Lab in Hong Kong University of Science and Technology)

Yeast Organelle

Figure 5. Super-resolution images of tubulin proteins

Tub1 and Tub4 in yeast spindle-pole body. GFP and RFP

tagged tubulin proteins were transfected into the yeast

cell, and further stained with GFP nanobody and RFP

nanobody, respectively. (Fig. a & b) widefield images. (Fig.

c & d) Corresponding super-resolution images, two-layer

structure of γ-tubulin complex was resolved.

(Images acquired with NBI SRiS 2.0, in cooperation with

Dr Yuanliang Zhai in Hong Kong University of Science and

Technology)

Mitochondria

Figure 6. 3D Three-dimensional super-resolut ion

images of mitochondria in COS7 cell. The mitochondrial

outer membrane TOM20 was labelled indirectly. The

mitochondria showed hollow tube structure.

(Images acquired with NBI SRiS 2.0 by NBI)

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Selected Publications by NBI SRiS Users

A. Cheng, T. Zhao, KH. Tse, HM. Chow, Y. Cui, L. Jiang, S. Du, MMT. Loy, K. Herrup, "ATM and ATR play

complementary roles in the behavior of excitatory and inhibitory vesicle populations." Proc Natl Acad Sci U S A.

2018 Jan 9;115(2):E292-E301.

H. Huang, X. Lin, Z. Liang, T. Zhao, S. Du, M.M.T. Loy, K.-O. Lai, A.K.Y. Fu, & N. Ip, “Cdk5-mediated

phosphorylation of liprina1 is critical for neuronal activity-dependent synapse development,”Proceedings of the

National Academy of Sciences. 2017 Aug 15;114(33):E6992-E7001.

Au Franco K.C., Jia Y., Jiang K. , Grigoriev I., Hau Bill K.T. , Shen Y. H. , Du S.W. , Akhmanova A., Qi Robert Z..”

GAS2L1 Is a Centriole-Associated Protein Required for Centrosome Dynamics and Disjunction.” Developmental

cell. 2017 Jan 9;40(1):81-94.

X. Gu, E. Zhao, T. Zhao, J.W.Y. Lam, S. Du, M.M.T. Loy, & B.Z. Tang, “A mitochondrion-specific photoactivatable

turn-on bioprobe for super-resolution imaging under physiological conditions without additives, “ Advanced

Materials, 2016, 28 (25), 5064-5071.

H. Wang, X. Zhuang, X. Wang, A.H.Y. Law, T. Zhao, S. Du, M.M.T. Loy, & L. Jiang, “A Distinct Pathway for Polar

Exocytosis in Plant Cell Wall Formation.” Plant Physiol. 2016 Oct;172(2):1003-1018.

G. Vail, A. Cheng, Y.R. Han, T. Zhao, S. Du, M.M.T. Loy, K. Herrup, & M. R. Plummer, “ATM protein is

located on presynaptic vesicles and its deficit leads to failures in synaptic plasticity," J Neurophysiology, 2016,

116 (1), 201-209.

Y. Du, H. Zhang, M. Zhao, D. Zou, and C. J. Xue, “Faster super-resolution imaging of high density molecules via

a cascading algorithm based on compressed sensing,” Opt. Express 23, 18563 (2015).

T. Zhao, Y. Wang, Y. Zhai, X. Qu, A. Cheng, S. Du, & M.M.T. Loy, “A user-friendly two-color super-resolution

localization microscope,” Opt. Express 23, 1879 (2015).

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System Configuration

SRiS 2.0 Series

SRiS 3.0 Series

Two-Channel Standard Two-Channel Advanced Two-Channel Professional

sCMOS detector sCMOS detector sCMOS detector

Microscope Microscope Microscope

Nano XYZ stage (Manual) Nano XYZ stage (Auto) Nano XYZ stage (Auto)

Opto-split ii Opto-split ii Opto-split ii

Optical element Optical element Optical element

656 and 750 nm lasers 656 and 750 nm lasers 656 and 750 nm lasers (Advanced)

Channel filter Channel filter Channel filter

Workstation Workstation Workstation

Optical table Optical table Optical table

NBI Active Locking System element NBI Active Locking System element NBI Active Locking System element

NBI outlook NBI outlook NBI outlook

NBI automatic control system NBI automatic control system NBI automatic control system

NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Advanced)

Three-Channel Standard Three-Channel Advanced Three-Channel Professional

sCMOS detector sCMOS detector sCMOS detector

Microscope Microscope Microscope

Nano XYZ stage (Manual) Nano XYZ stage (Auto) Nano XYZ stage (Auto)

Opto-split iii Opto-split iii Opto-split iii

Optical element Optical element Optical element

561, 656, and 750 nm lasers 561, 656, and 750 nm lasers 561, 656, and 750 nm lasers

Channel filter Channel filter Channel filter

Workstation Workstation Workstation

Optical table Optical table Optical table

NBI Active Locking System element NBI Active Locking System element NBI Active Locking System element

NBI outlook NBI outlook NBI outlook

NBI automatic control system NBI automatic control system NBI automatic control system

NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Standard) NBI Rohdea 3.0 (Advanced)

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BELIEVING

Email

imagingservice@nbi.hk

Web

www.nbi.hk

Address

Rm 505, Biotech Centre 1, No.9 Science Park West Avenue, Hong Kong Science Park, Shatin, N.T., Hong Kong

Tel

(852) 2688 0511

(86) 574 6302 8365