PR_7_ИФА
PR_8_Проточная цитометрия
Антитело
Варианты ИФА
Схема «Сэндвич» метода ИФА1. To coat the ELISA platewith diluted capture antibody and incubate overnight at 4°C.2. Wash the plate wells with ddH2O, wash with PBS-Triton twice.3. Block non-specific binding using 1% BSA/PBS and incubate for 30-60 minutes at RT.4. Wash plate. Add standards and 100ul of diluted samples to appropriate wells. 5. Incubate for 1 hour at RT. Wash.6. Add 100ul appropriate dilution of the secondary antibody conjugated with Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP) and incubate for 1 hour. Wash.7. Add 100ul of substrate to well and incubate at RT for 1 hour. (Add stopping solution)8. Read plates on an ELISA microplate reader.
Проточная цитометрияFlow cytometry
http://www.youtube.com/watch?v=TDRhCWaYRsg
Fluorescence Activated Cell Sorting (FACS)
Холостая проба
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