PR_7_ИФА

PR_8_Проточная цитометрия

Антитело

Варианты ИФА

Схема «Сэндвич» метода ИФА1. To coat the ELISA platewith diluted capture antibody and incubate overnight at 4°C.2. Wash the plate wells with ddH2O, wash with PBS-Triton twice.3. Block non-specific binding using 1% BSA/PBS and incubate for 30-60 minutes at RT.4. Wash plate. Add standards and 100ul of diluted samples to appropriate wells. 5. Incubate for 1 hour at RT. Wash.6. Add 100ul appropriate dilution of the secondary antibody conjugated with Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP) and incubate for 1 hour. Wash.7. Add 100ul of substrate to well and incubate at RT for 1 hour. (Add stopping solution)8. Read plates on an ELISA microplate reader.

Проточная цитометрияFlow cytometry

http://www.youtube.com/watch?v=TDRhCWaYRsg

Fluorescence Activated Cell Sorting (FACS)

Холостая проба