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Page 1: No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs

No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs

UT Tg

15’

Tg30

’Tg

60’

As15

’As

30’

As60

’U

T Tg15

’Tg

30’

Tg60

’As

15’

As30

’As

60’

HRI-/- #3 WT #7

eIF2a(P)

eIF2a(T)

UT Tg15

’Tg

30’

Tg60

’As

15’

As30

’As

60’

UT Tg15

’Tg

30’

Tg60

’As

15’

As30

’As

60’

HRI-/- #5 WT #11

Isabel Novoa & Yuhong Zhang

As: Sodium arsenite, 100M,

Tg: Thapsigargin, 100nM

Page 2: No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs

Genotyping of HRI locus

WT allele(P2/P6 PCR)

MUT alleleP2/P4 PCR

MUT (1.1Kb)

WT (0.6 Kb)

1.3Kb1.1Kb0.9Kb

0.6Kb

X174HaeIII

HR

I-/- #3H

RI-/- #5

WT

HR

I-/- #3H

RI-/- #5

WT


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