No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs
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No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs UT Tg15’ Tg30’ Tg60’ A s15 ’ As30 ’ A s60’ UT Tg15’ Tg30’ Tg60’ A s15’ A s30’ A s60’ HRI-/- #3 WT #7 eIF2a(P) eIF2a(T) UT Tg15’ Tg30’ Tg60’ As15’ As30’ As60’ UT Tg15’ Tg30’ Tg60’ A s15’ A s30’ As60’ HRI-/- #5 WT #11 Isabel Novoa & Yuhong Zhang As: Sodium arsenite, 100M, Tg: Thapsigargin, 100nM
description
No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs. HRI-/- #3. WT #7. HRI-/- #5. WT #11. Tg60’. Tg15’. Tg15’. Tg30’. Tg60’. Tg30’. Tg60’. Tg30’. Tg15’. Tg30’. Tg60’. Tg15’. As30’. As15’. As30’. As60’. As60’. As15’. As30’. As60’. As15’. As30’. As60’. As15’. UT. UT. - PowerPoint PPT Presentation
Transcript of No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs
No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs
UT Tg
15’
Tg30
’Tg
60’
As15
’As
30’
As60
’U
T Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
HRI-/- #3 WT #7
eIF2a(P)
eIF2a(T)
UT Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
UT Tg15
’Tg
30’
Tg60
’As
15’
As30
’As
60’
HRI-/- #5 WT #11
Isabel Novoa & Yuhong Zhang
As: Sodium arsenite, 100M,
Tg: Thapsigargin, 100nM
Genotyping of HRI locus
WT allele(P2/P6 PCR)
MUT alleleP2/P4 PCR
MUT (1.1Kb)
WT (0.6 Kb)
1.3Kb1.1Kb0.9Kb
0.6Kb
X174HaeIII
HR
I-/- #3H
RI-/- #5
WT
HR
I-/- #3H
RI-/- #5
WT