Fig. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs.

Chromatin (10 µg) from cortical neurons was subjected to IP with indicated antibodies (2 µg) and the presence of B-myb promoter sequence in the IPs (pellet) was detected by PCR (30 cycles). 0.5 µg chromatin before each IP was used as a positive contros (input). Antibodies used were KH95 (E2F1), L-20 (E2F2), C-18 (E2F3), C-20 (E2F4), and C-20 (E2F5).

E2F1

input

pellet

E2F2

E2F3

E2F4

E2F5