Fig. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs.
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g. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs. romatin (10 µg) from cortical neurons was subjected to IP with indicated antibodies (2 µg) and the pr myb promoter sequence in the IPs (pellet) was detected by PCR (30 cycles). 0.5 µg chromatin before e s used as a positive contros (input). Antibodies used were KH95 (E2F1), L-20 (E2F2), C-18 (E2F3), C- d C-20 (E2F5). E 2 F 1 input pellet E 2 F 2 E 2 F 3 E 2 F 4 E 2 F 5
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E2F3. E2F2. E2F1. E2F4. E2F5. pellet. input. Fig. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs. Chromatin (10 µg) from cortical neurons was subjected to IP with indicated antibodies (2 µg) and the presence of - PowerPoint PPT Presentation
Transcript of Fig. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs.
Fig. S1 Analysis of endogenous B-myb promoter occupancy by various E2Fs.
Chromatin (10 µg) from cortical neurons was subjected to IP with indicated antibodies (2 µg) and the presence of B-myb promoter sequence in the IPs (pellet) was detected by PCR (30 cycles). 0.5 µg chromatin before each IP was used as a positive contros (input). Antibodies used were KH95 (E2F1), L-20 (E2F2), C-18 (E2F3), C-20 (E2F4), and C-20 (E2F5).
E2F1
input
pellet
E2F2
E2F3
E2F4
E2F5
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