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Experimental Design for the
Effect of Inorganic Compound inthe Aerobic Granular Sludge
Development
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How to produce
aerobic granular with
high nutrient removal
efficiency
How to improve
biogranulationenhancement
What other things can
be used for
enhancement
What is the best
conditions to
optimize aerobic
granulation system
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In Reactor Granulation enhancement
1. Addition of inert nuclei, adsorbents, synthetic and
natural polymers
powder activated carbon (PAC), Zeolite, limestone, poly-
acryl-amide (synthetic polymer), chitosan (naturalpolymer)
2. Addition of divalent ions
calcium Ca2+,magnesium Mg2+ ,iron Fe2+ (Vlyssides 2009)
3. Influence of pulsation influentto promote hydrodynamic stress
4. Enhancement of substrate utilizing population
glucose, sucrose and lactate (Britz 2004)
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METHODOLGYIsolation of microbial from waste water sludge
The selection of type enhancement especially in inorganic matter
Identification of significant effecting variables to optimize performanceusing experimental design (response surface method)
Characteristics of the Extracellular Polymeric Substance and protein presentson the granulation
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Objectives:
To develop aerobic granular sludge by addition of nuclei inert anddivalent ions as enhancement in sequencing batch reactor systemwith the use of dye synthetic wastewater.
To characterize the physical, chemical and biological properties ofthe developed aerobic granular sludge from the enhancementprocesses
To investigate the properties of alginate-like exopolysaccharidesisolated from aerobic granular sludge.
To select the best conditions to optimize the enhancement processfor the granulation development using experimental design
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PROJECT ACTIVITIES2011 2012 2013
J A S O N D J F M A M J J A S O N D J F M A M J J
1. Literature studies
2. Methodology and Framework Design x
3. Identification variables that are expected toaffect granulation development
y Experimental design using Factorial
Design
y Inorganic variables (activated carbon,
zeolite, palm oil clinker etc)y Responses (coaggregation, surface
adsorbility, settleability, permeability,
porosity, productionof soluble and solubleEPS, Alginate- like-Exopolysaccharide
(ALE), Protein content etc.
x
4. Development of mathematical
modeling
y Response Surface Methodology (RSM)y Inorganic variables (activated carbon,
zeolite, palm oil clinker etc)y Responses (coaggregation, surface
adsorbility, settleability, permeability,
porosity, productionof soluble and solubleEPS, Alginate- like-Exopolysaccharide
(ALE), Protein content etc.
x
5.Optimization study x
6. Data Analysis
7. Report Writing x
Technology Transfer Activities
Attending workshop/seminar/conferences O O
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