8/6/2019 Development of a Method

1/6

Experimental Design for the

Effect of Inorganic Compound inthe Aerobic Granular Sludge

Development

8/6/2019 Development of a Method

2/6

How to produce

aerobic granular with

high nutrient removal

efficiency

How to improve

biogranulationenhancement

What other things can

be used for

enhancement

What is the best

conditions to

optimize aerobic

granulation system

8/6/2019 Development of a Method

3/6

In Reactor Granulation enhancement

1. Addition of inert nuclei, adsorbents, synthetic and

natural polymers

powder activated carbon (PAC), Zeolite, limestone, poly-

acryl-amide (synthetic polymer), chitosan (naturalpolymer)

2. Addition of divalent ions

calcium Ca2+,magnesium Mg2+ ,iron Fe2+ (Vlyssides 2009)

3. Influence of pulsation influentto promote hydrodynamic stress

4. Enhancement of substrate utilizing population

glucose, sucrose and lactate (Britz 2004)

8/6/2019 Development of a Method

4/6

METHODOLGYIsolation of microbial from waste water sludge

The selection of type enhancement especially in inorganic matter

Identification of significant effecting variables to optimize performanceusing experimental design (response surface method)

Characteristics of the Extracellular Polymeric Substance and protein presentson the granulation

8/6/2019 Development of a Method

5/6

Objectives:

To develop aerobic granular sludge by addition of nuclei inert anddivalent ions as enhancement in sequencing batch reactor systemwith the use of dye synthetic wastewater.

To characterize the physical, chemical and biological properties ofthe developed aerobic granular sludge from the enhancementprocesses

To investigate the properties of alginate-like exopolysaccharidesisolated from aerobic granular sludge.

To select the best conditions to optimize the enhancement processfor the granulation development using experimental design

8/6/2019 Development of a Method

6/6

PROJECT ACTIVITIES2011 2012 2013

J A S O N D J F M A M J J A S O N D J F M A M J J

1. Literature studies

2. Methodology and Framework Design x

3. Identification variables that are expected toaffect granulation development

y Experimental design using Factorial

Design

y Inorganic variables (activated carbon,

zeolite, palm oil clinker etc)y Responses (coaggregation, surface

adsorbility, settleability, permeability,

porosity, productionof soluble and solubleEPS, Alginate- like-Exopolysaccharide

(ALE), Protein content etc.

x

4. Development of mathematical

modeling

y Response Surface Methodology (RSM)y Inorganic variables (activated carbon,

zeolite, palm oil clinker etc)y Responses (coaggregation, surface

adsorbility, settleability, permeability,

porosity, productionof soluble and solubleEPS, Alginate- like-Exopolysaccharide

(ALE), Protein content etc.

x

5.Optimization study x

6. Data Analysis

7. Report Writing x

Technology Transfer Activities

Attending workshop/seminar/conferences O O