# CELL LINE TYPE GROWTH TYPE MEDIUM1 MCF-7 Breast cancer Adherent EMEM2 HeLa Cervical cancer Adherent MEM3 SiHa Cervical cancer Adherent EMEM4 T47D Breast cancer Adherent RPMI 16405 HCC2935 Lung Cancer Adherent RPMI 16406 DMS 79 Lung Cancer suspension RPMI 16407 MCF-12A Normal breast tissue Adherent DMEM8 CCD 18Lu Lung fibroblast Adherent EMEM

Reagents required

1. Trypsin2. EDTA3. Sodium Bi carbonate4. PBS5. T25 FLASK(vented cap)6. FBS7. Penstrep8. Syringe filter (0.2 µm size)9. Mutli wel plate(96)-flat10. Falcon tube (15ml)11. Hand clouse pack12. Tips (1ml,100micro l,0.5ml,0.005ml)13. Cryogenic vial14. Sodium hypo chloride 15. DMSO16. Ethanol17. Tryphan blue18. Heamocytometer19. MTT