CELL LINE Chemicals
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# CELL LINE TYPE GROWTH TYPE MEDIUM 1 MCF-7 Breast cancer Adherent EMEM 2 HeLa Cervical cancer Adherent MEM 3 SiHa Cervical cancer Adherent EMEM 4 T47D Breast cancer Adherent RPMI 1640 5 HCC2935 Lung Cancer Adherent RPMI 1640 6 DMS 79 Lung Cancer suspension RPMI 1640 7 MCF-12A Normal breast tissue Adherent DMEM 8 CCD 18Lu Lung fibroblast Adherent EMEM Reagents required 1. Trypsin 2. EDTA 3. Sodium Bi carbonate 4. PBS 5. T25 FLASK(vented cap) 6. FBS 7. Penstrep 8. Syringe filter (0.2 µm size) 9. Mutli wel plate(96)-flat 10. Falcon tube (15ml) 11. Hand clouse pack 12. Tips (1ml,100micro l,0.5ml,0.005ml) 13. Cryogenic vial 14. Sodium hypo chloride 15. DMSO 16. Ethanol 17. Tryphan blue 18. Heamocytometer 19. MTT
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Transcript of CELL LINE Chemicals
# CELL LINE TYPE GROWTH TYPE MEDIUM1 MCF-7 Breast cancer Adherent EMEM2 HeLa Cervical cancer Adherent MEM3 SiHa Cervical cancer Adherent EMEM4 T47D Breast cancer Adherent RPMI 16405 HCC2935 Lung Cancer Adherent RPMI 16406 DMS 79 Lung Cancer suspension RPMI 16407 MCF-12A Normal breast tissue Adherent DMEM8 CCD 18Lu Lung fibroblast Adherent EMEM
Reagents required
1. Trypsin2. EDTA3. Sodium Bi carbonate4. PBS5. T25 FLASK(vented cap)6. FBS7. Penstrep8. Syringe filter (0.2 µm size)9. Mutli wel plate(96)-flat10. Falcon tube (15ml)11. Hand clouse pack12. Tips (1ml,100micro l,0.5ml,0.005ml)13. Cryogenic vial14. Sodium hypo chloride 15. DMSO16. Ethanol17. Tryphan blue18. Heamocytometer19. MTT
