In cases of serious bacterial infections the timely administration of an effective antibiotic therapy is associated with an increase in disease resolution and subsequent patient survival. For this reason, the microbiology laboratory has to provide "clinically useful results" in order to guide the clinician to choose the most appropriate antibiotic therapy as soon as possible. Rapid Antimicrobial Susceptibility Test (AST) results facilitate effective treatment, reduce the number of laboratory tests ordered, days of hospitalization and Public Health costs.In addition, they are useful to monitor the evolution of resistant bacteria.
Alifax clinical susceptibility testing is the unique test that gives results in only 3 hours
ANTIMICROBIALSUSCEPTIBILITY TESTING
The Alifax systems detect if a bacteria is sensitive or resistant to selected antibiotics by comparing growth curves of the same bacterial suspension inoculated in vials with and without (reference vial) drugs. REFERENCE RESISTANT SENSITIVE
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LIGHT SCATTERING TECHNOLOGY APPLIED TO SUSCEPTIBILITY TESTING
Sidecar, Alfred 60AST and HB&L are conceived in order to properly monitor the turbidity levels of the samples simultaneously to the culture test. Positive samples are flagged when the turbidity reaches the 0.5 McFarland by McFarland Monitor. Monomicrobial samples can then be tested with a customized antibiotic panel providing results within few hours.
The results are expressed in percentage of resistance to the antibiotic and classified as Resistant, Intermediate or Sensitive.
Alifax AST is applicable on positive cultures obtained using the Uro-Quick Screening Kit (Code SI 390.900), Automation kit (Code SI 1201.900), HB&L™ Culture Kit (Code SI 405.901), bacterial suspensions from isolated colonies and now also on positive hemoculture.
Comparative studies vs the Kirby Bauer reference system(1,2)
and other automated systems
(3,4,5) show high correlations.
Results are available in 3 hours.
EACH ANTIBIOTIC IS INDIVIDUALLY MARKED
1 AMIKACIN ENTEROBACTERIACEAE SI 956-AMK
2 AMIKACIN PSEUDOMONAS NN) SI 978-AMK
3 AMIKACIN STAPHYLOCOCCI SI 981-AMK
4 AMPICILLIN ENTEROBACTERIACEAE SI 954-AMP
5 AMPICILLIN ENTEROCOCCI SI 955-AMP
6 AMPICILLIN-SULBACTAM ENTEROBACTERIACEAE SI 997-AMS
7 AZTREONAM ENTEROBACTERIACEAE SI 957-ATM
8 CEFOTAXIME SI 959-CTX
9 CEFOXITIN CNS SI 962-FOX
10 CEFOXITIN STAPH. AUREUS SI 961-FOX
11 CEFTAZIDIME ENTEROBACTERIACEAE SI 949-CAZ
12 CEFTAZIDIME PSEUDOMONAS SI 950-CAZ
13 CEFTRIAXONE SI 951-CRO
14 CEFUROXIME SI 960-FUR
15 CIPROFLOXACIN SI 963-CIP
16 CLINDAMYCIN STAPHYLOCOCCI SI 964-CLI
17 COLISTIN PSEUDOMONAS SI 983-CST
18 COLISTIN ENTEROBACTERIACEAE SI 984-CST
19 COTRIMOXAZOLE ENTEROBACTERIACEAE SI 965-SXT
20 COTRIMOXAZOLE STAPHYLOCOCCI SI 982 - SXT
21 DAPTOMYCIN STAPHYLOCOCCI SI 986-DAP
22 GENTAMICIN SI 967-GEN
23 GENTAMICIN HLAR SI 999-GEN
24 GENTAMICIN STAPHYLOCOCCI SI 968-GEN
25 LEVOFLOXACIN SI 969-LEV
26 LINEZOLID SI 970-LZD
27 MEROPENEM ENTEROBACTERIACEAE SI 971-MEM
28 MEROPENEM PSEUDOMONAS SI 979-MEM
29 PIPERACILLIN-TAZOBACTAM ENTEROBACTERIACEAE SI 953-TZP
30 PIPERACILLIN-TAZOBACTAM PSEUDOMONAS SI 952-TZP
31 RIFAMPICIN SI 996-RIF
32 TEICOPLANIN CNS SI 976-TEI
33 TEICOPLANIN S. AUREUS AND ENTEROCOCCI SI 975-TEI
34 VANCOMYCIN CNS AND ENTEROCOCCI SI 974-VAN
35 VANCOMYCIN S. AUREUS SI 973-VAN
EUCAST LYOPHILISED ANTIBIOTICS Code
1 AMIKACIN SI 801-AMK
2 AMOXICILLIN-CLAVULANATE STAPHYLOCOCCI SI 802-AMC
3 AMPICILLIN ENTEROBACTERIACEAE SI 803-AMP
4 AMPICILLIN ENTEROCOCCI SI 804-AMP
5 AZTREONAM ENTEROBACTERIACEAE SI 805-ATM
6 AZTREONAM PSEUDOMONAS SI 806-ATM
7 CEFOTAXIME ENTEROBACTERIACEAE SI 807-CTX
8 CEFOXITIN STAPH. AUREUS SI 809-FOX
9 CEFOXITIN CNS SI 810-FOX
10 CEFTAZIDIME ENTEROBACTERIACEAE SI 811-CAZ
11 CEFTAZIDIME PSEUDOMONAS SI 812-CAZ
12 CEFTRIAXONE ENTEROBACTERIACEAE SI 813-CRO
13 CEFUROXIME ENTEROBACTERIACEAE SI 815-FUR
14 CIPROFLOXACIN SI 817-CIP
15 CLINDAMYCIN STAPHYLOCOCCI SI 818-CLI
16 COTRIMOXAZOLE SI 820-SXT
17 DAPTOMYCIN STAPHYLOCOCCI SI 837-DAP
18 FOSFOMYCIN SI 821-FOS
19 GENTAMICIN SI 822-GEN
20 LEVOFLOXACIN SI 823-LEV
21 LINEZOLID SI 824-LZD
22 MEROPENEM ENTEROBACTERIACEAE SI 825-MEM
23 MEROPENEM PSEUDOMONAS SI 826-MEM
24 NORFLOXACIN SI 828-NOR
25 OXACILLIN STAPH. AUREUS SI 829-OXA
26 PIPERACILLIN-TAZOBACTAM ENTEROBACTERIAEAE SI 830-TZP
27 PIPERACILLIN-TAZOBACTAM PSEUDOMONAS SI 831-TZP
28 TEICOPLANIN STAPHYLOCOCCI SI 832-TEI
29 TEICOPLANIN ENTEROCOCCI SI 833-TEI
30 VANCOMYCIN STAPH. AUREUS SI 834-VAN
31 VANCOMYCIN CNS AND ENTEROCOCCI SI 835-VAN
CLSI LYOPHILISED ANTIBIOTICS Code
Thanks to the unique application positive blood cultures can be tested with Alifax AST kits dramatically reducing the analytical turnaround time and giving results in few hours(4,5,6).
Alifax AST gives results about antibiotics effectiveness 24-48 hours sooner than traditional methods.
ALIFAX SUSCEPTIBILITY TESTING ON POSITIVE HEMOCULTURE SAMPLES
Each Alifax AST antibiotic kit contains: • 5 vials of lyophilized antibiotic• 2 Regenerating Solution• 1 Mic CARD
30 TEST KIT
RELATED KITS
FOR AUTOMATION ON ALFRED 60AST AND SIDECAR1. AUTOMATION KIT Code SI 1201.9002. G-NF AST BROTH KIT Code SI 1201.9053. EMPTY VIALS Code SI AST-EV
FOR MANUAL EXECUTION ON HB&L1. AUTOMATION KIT Code SI 1201.9102. G-NF AST BROTH KIT Code SI 1201.905
SHELF LIFEFrom production: 8-12 monthsFrom reconstitution: 4-7 days
STORAGE CONDITIONSRefrigerated temperature (+4÷8 °C)
1. POSITIVE SAMPLE SELECTION AND MICROSCOPE EVALUATION
Ref. 6
2. 10 µl BACTERIAL SUSPENSION IN A HB<M ENRICHMENT VIAL + DEB SUPPLEMENT
UNTIL THE 0.5 MCFARLAND
3. ALIFAX AST WITH A CUSTOMIZED ANTIBIOTIC PANEL FOLLOWING:
- GRAM INDICATION - ADMINISTERED DRUG INFORMATION - THERAPEUTIC PROTOCOL DATA AND GUIDE LINE
BACTERIAL YEALD FROM ENRICHMENT VIAL CAN BE USED FOR MASS SPECTROMETRY IDENTIFICATION
4. RESULTS IN 3 HOURSR
efer
ence
0.5
Mc
Farl
and
Anti
bio
tic
1
Anti
bio
tic
2
Anti
bio
tic
3
Anti
bio
tic
4
Anti
bio
tic
5
NEW
Bibliography1- S. Roveta et al. (Microbiology Institute, Genoa University, Italy) "Antibiotic susceptibility tests directly on urine samples by Uro-Quick, a rapid automated system", J Chemother. 2006 Feb;18(1): 12-92- E. Pezzati et al. (Microbiology Institute, Genoa University, Italy) " Evaluation of the Uro-Quick system for antibiotic susceptibility tests of strains collected from intensive care units" Annals of Microbiology 2006, 56(2):179-1833- A. Tessari et al. (Mic. Dep., Padova University Hospital ,Padova, Italy) Evaluation of a new rapid method for microbial growth analysis and antimicrobial susceptibility test in human biological fluids” Abstract, 17th ECCMID, München, 31 March-3 April 2007.4- V. Kroumova et al. (Microbiology and Virology laboratory from university mayor hospital of charity of Novara, Italy) “Preliminary indications for antibiotic susceptibility tests in less than six hour in positive blood cultures” Microbiologia Medica, Anno 2010, Vol. 25, N° 1, 24-265- S. Barocci et al. (Microbiology Unit, Clinical Pathology Laboratories ASUR 4 Senigallia (AN), Italy) “HB&L System: rapid determination of antibiotic sensitivity of bacteria isolated from blood cultures” Microbiologia Medica, Anno 2010, Vol. 25, N° 1, 60-636- V. Rondinelli et al.“ New method for rapid Susceptibility Testing on blood culture with HB&L system: preliminary data” (Mic. e Vir. Lab Azienda Ospedaliera Pugliese-Ciaccio, Catanzaro) Microbiologia Medica, 2010, Vol. 25, N°4, 238-243
Mc FARLANDMONITOR
BACTERIALCULTURE
MASSSPECTROMETRYIDENTIFICATION
SUSCEPTIBILITYTESTING
FULL INTEGRATED TECHNOLOGIES
1DAYRESULT
The Alifax systems determine if a bacteria is sensitive or resistant to selected antibiotics by comparing growth curves of the same bacterial suspension inoculated in vials with and without (reference vial) drugs.
Thanks to the new revolutionary application on HB&L and Alfred 60, positive blood cultures can be tested with Alifax AST kits dramatically reducing the analytical turnaround time and giving results in few hours(4,5,6).
Alifax AST gives results about antibiotics effectiveness 24-48 hours sooner than traditionalmethods.
ALIFAX ANTIMICROBIAL SUSCEPTIBILITY TESTING
ALIFAX SUSCEPTIBILITY TESTING ON POSITIVE HEMOCULTURE SAMPLES
Each Alifax AST antibiotic kit contains: • 10 vials of lyophilized antibiotic• Regenerative Solution• 60 blue cap disposable vials with coloured glass
SHELF LIFEFrom production: 8 months at +2÷8 °C.From reconstitution: 7 days at +4 °CSTORAGE CONDITIONSRefrigerated temperature (+2÷8 °C)RELATED KITAST Mc Farland Kit Code SI 912 SVR
1. POSITIVE SAMPLE SELECTION AND MICROSCOPE EVALUATION
Ref. 6
REFERENCE RESISTANT SENSITIVE
2. DILUTION 1:10 OF POSITIVE BLOOD CULTURE BROTH IN SALINE SOLUTION
3. 500 µl SUSPENSION DILUTION IN A HB<M CULTURE VIAL + DEB SUPPLEMENT
UNTIL THE 0.5 MCFARLAND
4. ALIFAX AST with a customized antibiotic panel following:
- Gram indication - Administered drug information - Therapeutic protocols data and guide line
5. RESULTS IN 3 HOURSR
efer
ence
1:10
0.5
Mc
Far
land
Ant
ibio
tic 1
Ant
ibio
tic 2
Ant
ibio
tic 3
Ant
ibio
tic 4
Ant
ibio
tic 5
NEW
Bibliography1- S. Roveta et al. (Microbiology Institute, Genoa University, Italy) "Antibiotic susceptibility tests directly on urine samples by Uro-Quick, a rapid automated system", J Chemother. 2006 Feb;18(1): 12-92- E. Pezzati et al. (Microbiology Institute, Genoa University, Italy) " Evaluation of the Uro-Quick system for antibiotic susceptibility tests of strains collected from intensive care units" Annals of Microbiology 2006, 56(2):179-1833- A. Tessari et al. (Mic. Dep., Padova University Hospital ,Padova, Italy) Evaluation of a new rapid method for microbial growth analysis and antimicrobial susceptibility test in human biological fl uids” Abstract, 17th ECCMID, München, 31 March-3 April 2007.4- V. Kroumova et al. (Microbiology and Virology laboratory from university mayor hospital of charity of Novara, Italy) “Preliminary indications for antibiotic susceptibility tests in less than six hour in positive blood cultures” Microbiologia Medica, Anno 2010, Vol. 25, N° 1, 24-265- S. Barocci et al. (Microbiology Unit, Clinical Pathology Laboratories ASUR 4 Senigallia (AN), Italy) “HB&L System: rapid determination of antibiotic sensitivity of bacteria isolated from blood cultures” Microbiologia Medica, Anno 2010, Vol. 25, N° 1, 60-636- V. Rondinelli et al.“ New method for rapid Susceptibility Testing on blood culture with HB&L system: preliminary data” (Mic. e Vir. Lab Azienda Ospedaliera Pugliese-Ciaccio, Catanzaro) Microbiologia Medica, 2010, Vol. 25, N°4, 238-243
Rev
. 07.
10.1
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ALIFAX S.p.A. Via F. Petrarca, 2/1 35020 Polverara - Padova, ITALY Tel. +39 049 0992000 Fax +39 049 5855434 [email protected] www.alifax.comEntered in Padova companies register at the n. 04337640280. Company stock € 6.000.000 entirely deposited Company with single partner
Rev
. 27.
10.1
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ALIFAX S.r.l. Via F. Petrarca, 2/1 - 35020 Polverara - Padova, ITALY - Tel. +39 049 0992000 - Fax +39 049 5855434 - [email protected] - www.alifax.comEntered in Padova companies register at the n. 04337640280. Company stock E 10.000.000 entirely deposited Company with single partner
SI 1201.900 SI 1201.905 SI 1201.910
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