160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coatedglass slide, then the surface is “blocked” with BSA

106 PBMCs are added to the slide surface and allowed to settle

Antibodies ‘capture’ only PBMCs that express corresponding CD antigens. Washing removesthe rest

PBMCs bind at different CD antibody spots in proportional to the number of cells bearing that epitope, and the number of surface CD epitopes per cell

STEPS in Preparing a PBMC Array11

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