160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the
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160 anti-CD antibodies are robotically arrayed in a nitrocelllulose- coated glass slide, then the surface is “blocked” with BSA 10 6 PBMCs are added to the slide surface and allowed to settle Antibodies ‘capture’ only PBMCs that express corresponding CD antigens. Washing removes the rest PBMCs bind at different CD antibody spots in proportional to the number of cells bearing that epitope, and the number of surface CD epitopes per STEPS in Preparing a PBMC Array 1 2 3 4
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STEPS in Preparing a PBMC Array. 160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the surface is “blocked” with BSA. 1. 10 6 PBMCs are added to the slide surface and allowed to settle. 2. - PowerPoint PPT Presentation
Transcript of 160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coated glass slide, then the
160 anti-CD antibodies are robotically arrayed in a nitrocelllulose-coatedglass slide, then the surface is “blocked” with BSA
106 PBMCs are added to the slide surface and allowed to settle
Antibodies ‘capture’ only PBMCs that express corresponding CD antigens. Washing removesthe rest
PBMCs bind at different CD antibody spots in proportional to the number of cells bearing that epitope, and the number of surface CD epitopes per cell
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