Winning Westerns—great results · Mini Blotting Package with Semi-Dry Unit 03-500-805 Ordering...
Transcript of Winning Westerns—great results · Mini Blotting Package with Semi-Dry Unit 03-500-805 Ordering...
Winning Westerns—great resultsPremier products for Western blotting
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Your Optimized Western Blotting Products Portfolio
One of the greatest challenges in advancing research is obtaining consistent, quality results. In Western blotting, several factors contribute to the success of experiments, including the quality of the protein samples, transfer efficiency of the proteins to the membrane and the reagents used throughout the protocol. Millipore® and Hoefer® offer an extensive array of Electrophoresis and Western blotting products that are optimized to work synergistically, providing strong specific signals and low background to help you quickly produce reproducible publication-quality results.
Description Size* Qty. Cat. No.
Immobilon P: PVDF 0.45μm 26.5cm x 3.75m 1 Roll IPVH00010
Immobilon PSQ: PVDF 0.2μm 26.5cm x 3.75m 1 Roll ISEQ00010
Immobilon FL: PVDF 0.45μm 26.5cm x 3.75m 1 Roll IPFL00010* Other sizes available
Hoefer® Nitrocellulose Membranes Ideal for Western blotting and other immunoblotting techniques
• Superior strength—withstand aggressive handling or can be used with automated equipment without breaking or tearing • Membrane binds a wide range of fragment sizes and can still be easily blocked for low background • Uniform pore structure provides consistent flow and diffusion rates
Description Qty. Cat. No.
0.22μm pore size 30cm x 3m 1 Roll 03-500-600
0.45μm pore size 30cm x 3m 1 Roll 03-500-601
0.45μm pore size 8cm x 9.5cm 10 Pk. 03-500-604
0.45μm pore size 16cm x 16cm 10 Pk. 03-500-602
0.45μm pore size 23.5cm x 20cm 10 Pk. 03-500-603
Millipore Immobilon™ Western Blotting Transfer Membranes Millipore offers three varieties of PVDF transfer membranes, each optimized for different protein blotting applications. All have exceptional handling characteristics and are compatible with a variety of detection chemistries.
• Excellent protein retention, physical strength and broad chemical compatibility• Compatibility with a variety of staining applications and reprobing techniques
Description Detection Method Qty. Cat. No.
Bløk-CH Reagent Chemiluminescence 500mL/bottle WBAVDCH01
Bløk-FL Reagent Fluorescence 500mL/bottle WBAVDFL01
Bløk-PO Reagent Phosphoprotein 500mL/bottle WBAVDP001
Millipore Bløk™ Noise-Cancelling ReagentsBløk reagents are a family of uniquely formulated inorganic blocking solutions that provide numerous benefits including:
• Reduced background for chemiluminescent or fluorescent detection• Pre-diluted synthetic reagents that are ready to use and stable at room temperature• Unique formulation for detection of phosphorylated proteins
Ordering Information:
Description Cat. No.
Mini Blotting Package with Semi-Dry Unit 03-500-805
Ordering Information:
Description Cat. No.
Mini Transfer Tank Combination Package 03-500-804
Package Includes:
Description Cat. No.
Hoefer Mighty Small™ Mini Deluxe Vertical Unit
03-500-111
Semi-Dry Transfer Unit 03-500-612
300V, 500mA, 90W Power Supply PS300BNA
Package Includes:
Description Cat. No.
Hoefer Mighty Small™ Mini Deluxe Vertical Unit
03-500-111
TE 22 Transfer Tank 03-500-216
300V, 500mA, 90W Power Supply PS300BNA
Your Optimized Western Blotting Products Portfolio
Save Time and Money with Complete Mini Western Blotting Packages*
Millipore Luminata™ HRP Chemiluminescence Detection Reagents The new family of Luminata HRP chemiluminescent substrates is premixed for convenience. Just pour directly onto the Western membrane without worrying about a pipetting error.
• Premixed for convenience to avoid cross-contamination of the bottles and stable at room temperature• High signal intensity and low background to enable detection of low protein concentrations• Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents
Description Qty. Cat. No.
Luminata Classico HRP Chemiluminescence Detection Reagent
100mL WBLUC0100
500mL WBLUC0500
Luminata Crescendo HRP Chemiluminescence Detection Reagent
100mL WBLUR0100
500mL WBLUR0500
Luminata Forte HRP Chemiluminescence Detection Reagent
100mL WBLUF0100
500mL WBLUF0500
Description Qty. Cat. No.
SNAP i.d. Protein Detection System WBAVDBASE
SNAP i.d. Consumables and Accessories
Single Blot Holder 30/Pk. WBAVDBH01
Double Blot Holder 30/Pk. WBAVDBH02
Triple Blot Holder 20/Pk. WBAVDBH03
Antibody Collection Tray 20/Pk. WBAVDABTR
SNAP i.d. Blot Roller 1/Pk. WBAVDROLL
Millipore SNAP i.d.™ Protein Detection System Rapid immunodetection in just 22 minutes!The SNAP i.d. Protein Detection system incorporates a vacuum that actively drives reagents through the blotting membrane.
• Fast immunodetection enabling more experiments in less time• Superior washes for a lower background• Equivalent or better signal to noise ratios than traditional methods
Hoefer TE70XP Semi-Dry Transfer UnitPerform uniform transfers of proteins from polyacrylamide gels in less than one hour • Built-in intelligent power supply prevents blots from dehydrating• Can run up to four mini gels at once for higher throughput• Easy operation and cleanup
Hoefer TE22 Mighty Small Transfer TankSafe, economical unit requires minimal current and minimal buffer • Built-in alumina heat exchanger permits efficient transfers in less than one hour • Platinum electrode ensures uniform electrical field for complete and even transfers • Hinged, color-coded cassettes enable simple loading and easy orientation of gel sandwich
Hoefer 300V, 500mA, 90W Power SupplySmall footprint • 90 watts, 500mA and 300 volts enables it to run four mini tanks at once• Low volt and amperage detection limits (can transfer a low amperage or voltage)• Large LED screen for easy viewing
Hoefer SE260 Mighty Small Mini Deluxe Vertical Unit Combine the advantages of a small format and efficient cooling for rapid screening of protein.Ideal for protein separation in 1-D and second dimension 2-D gels• Fits all 10x10cm precast gels• Built-in cooling for faster runs and sharper bands• Leakproof casting with the SE245 casting system
*Larger formats available