We wrote the book. You published the...
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Transcript of We wrote the book. You published the...
Since it was first printed in 1975, the Molecular Probes® Handbook has served
as a reference guide for fluorescent probes and labeling technologies.
Thanks to your publications, it now describes the most peer-referenced
fluorescent reagents used in life science research.
Find out why Molecular Probes® reagents are preferred byresearchers worldwide at lifetechnologies.com/probes
We wrote the book. You published the papers.
This. Is. Molecular Probes.
For Research Use Only. Not for use in diagnostic procedures. Life Technologies is a Thermo Fisher Scientific brand. © 2014 Thermo FisherScientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. CO16164 0214
Clone withConfidence.Whether you are performing your first cloning
experiment, or constructing multi-fragment
gene assemblies, NEB® has the solution for you.
Our high quality reagents are available for every
workflow, and include specialized enzymes,
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Gibson Assembly®. When you are looking to
clone with confidence, think of NEB.
Explore the wise choice atCloneWithNEB.com.
Visit CloneWithNEB.com to view
online tutorials describing various
cloning workflows.
NEW ENGLAND BIOLABS® and NEB® are registered trademarks of New England Biolabs, Inc.GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
The BD Accuri C6 is making it even easier toapply the power of flow cytometry to yourresearch with free software templates and ready-to-go reagent kits that simplify the assessmentof cell cycle and DNA status studies involvingpropidium iodide, 7-amino actinomycin D, andbromodeoxyuridine. You get more insight andeasier workflow, whether you’re a flow expert orhave never used flow cytometry before.
These templates feature predefined workspacethat speeds setup and analysis. Applying thepower of flow cytometry, the BD Accuri C6uses less samples and delivers greater accuracy.
Several kits are available including BD Cycletest™Plus DNA Kit that uses PI and other activeagents to obtain precise ploidy and cell cyclemeasurements using isolated cell nuclei andBD Pharmingen™ BrdU Kits that use 7-AADand BrdU to provide high resolution cell cyclemeasurements.
Find out more about how the BD Accuri C6 putsthe power of 4-color cell analysis within reach bybeing easy to use, simple to set up and maintain,transportable, and affordable.
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Accurate, Fast, Cell Cycle StudiesMade easy using the BD Accuri™ C6 Personal Flow Cytometer.
Arguably, as easy asCell Cycle is going to get.
Class 1 Laser Product.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2013 BD
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2013 Winner
Dr. Michael Yartsev
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Princeton Neuroscience
Institute
Eppendorf & Science Prize for Neurobiology
The annual Eppendorf & Science Prize for Neurobiology,
an international award, honors young scientists for their
outstanding contributions to neurobiological research
based on methods of molecular and cell biology.
The winner and finalists are selected by a committee
of independent scientists, chaired by Science’s Senior
Editor, Dr. Peter Stern. To be eligible, you must be
35 years of age or younger.
You could be next to win this prize and to receive
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Call for EntriesApplication Deadline
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BRAIN AND BEHAVIOR
Winner: Kirollos Bechay, University
of California, Irvine
Histone-deactylation is a Critical
Mechanism of Auditory Learning and
Cortical Plasticity
Honorable Mention: Eric W. Hendricks
and Austin J. Tofte, Eastern Illinois
University
Estrogen Drastically Increases
Mitochondrial Respiration in Alzheimer’s
Mouse Model
CELLULAR AND MOLECULAR
BIOLOGY
Winner: Zoey Adele Prokopiak, Slippery
Rock University, and Kevin Houlihan,
University of North Carolina, Chapel Hill
Cooperative Effects of Hydrogen Bonds in
Protein Structure Prediction
Honorable Mention: Akihito Fukudome,
Emre Aksoy, and Krishna Kumar,
Texas A&M University
A Plant RNA Polymerase II CTD
Phosphatase Regulates Xenobiotic Stress
Response
DEVELOPMENTAL BIOLOGY,
PHYSIOLOGY, AND IMMUNOLOGY
Winner: Neto Sosa and Yoon Kyung Kim,
University of California, Irvine
Production of Immunomodulatory
Protein CD200 from E. coli
for Coating Biomedical Implants
Honorable Mention: Shannon K. Boi,
Joel R. Dennison, and Bailey R. Rhoads,
Northern Illinois University
Transcription Factor GLI3 is a Novel
Candidate Effector of Toll-like Receptor
4 Signaling
ENVIRONMENT AND ECOLOGY
Winner: Rebeca Flores, University of
California, Irvine
Pattern of Resistance and Variability
of Tetracycline Resistance Genes in
Staphylococcus
Honorable Mention: Daylen T. Isaac,
Heritage University
Nutrient Sorption of Biochar
MATH, TECHNOLOGY, AND
ENGINEERING
Winner:Muneeb Mohideen,
Yale University
Polyplexes Formed from NovelPoly(lactone-ω-hydroxyaminoesters)as Gene Delivery Vehicles
Honorable Mention: Ashwin Chander
Ramesh, Rochester Institute of Technology
Power Generation Using SimplifiedModels of Tethered Airfoils
MEDICINE AND PUBLIC HEALTH
Winner: Stephan Timothy Kudlacek,
University of California, Irvine
Asenapine Reverses Efflux Mediated
Ciprofloxacin Resistance in
Staphylococcus aureus
Honorable Mention: Disa Patel,
Northern Illinois University
Assessing Individual Interventions and
Need for Policy Interventions to Reduce
PM Exposure
PHYSICAL SCIENCES
Winner: Lauren R. Grabstanowicz and
Robert M. Rickard, Northern Illinois
University
Oxidative Method and Analysis of
Self-Doped Black TiO2 for Visible Light
Photoactivity
Honorable Mention: Di Liu, Usman Akhter,
and Timothy M. Cronin, University of
Chicago
Creating Complex Molecular Topologies
by Configuring DNA Four-Way Junctions
SCIENCE AND SOCIETY
Winner: Anika Larson, Arizona State
University
Choosing Animal Models for Translational
Neuroscience
Honorable Mention:Michael A. Bilek,
Sunil Thapa, and Erin R. Tuegel,
Eastern Illinois University
White-rot Fungi as Biological
Pretreatment Agents for
Biomass-to-Bioenergy Strategies
SOCIAL SCIENCES
Winner: Christina S. Melki,
Indiana University
Evaluation of Clicker Use and Student
Success in College Science Classrooms
Honorable Mention: Rebecca Harkness
and Will G. Russell, Arizona State
University
The Diverse Social Landscape of
Prehispanic Southwest New Mexico
AAAS GENERAL POSTER SESSION
ATTENDEES’ CHOICE
Winner: Gang Chen, Di Liu, and Timothy
Cronin, University of Chicago
Programmable Assembly of Colloidal
Nanoparticles with Specific Bonding
Directionality
Congratulations to the 2014 AAAS Student Poster Competition Winners
13 - 17 F E B R U A R Y • C H I C A G O
ANNUALMEETING2014AAAS
DISCOVERY AND INNOVATION
M E E T I N G G L O B A L C H A L L E N G E S :
The 2014 Student Poster Competition took place at the AAAS Annual Meeting in Chicago, 13–17 February.
The student winners’ work displayed originality and understanding that set them apart from their peers.
The 2015 poster entry site will open in mid-May 2014.
Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.
Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organiza-
tions are featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of
any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.
CANCER PANEL
The xGen Acute Myeloid Leukemia Cancer Panel v1.0 consists of 11,743 xGen Lockdown Probes, targeting over 260 clinically relevant genes that were found to be mutated in a study of 200 patients with acute myeloid leukemia (AML), and published by The Cancer Genome Atlas consortium. The AML Cancer Panel is used for enriching the genome for regions of interest before performing next generation sequencing (NGS). It can, therefore, be used to study disease occurrence and progression, and help with the development of better targeted therapies. Target enrichment enables users to focus their sequencing efforts on specific regions of the genome, providing a cost-effective and reliable alternative to whole-genome sequencing, making NGS more accessible to researchers. By including only relevant xGen Lockdown Probes, the AML Cancer Panel enables high coverage of targeted regions, with minimal GC bias. Integrated DNA Technologies For info: 800-328-2661 www.idtdna.com
REACTION VESSEL
The new ReactoMate Pilot jacketed reaction vessel offers precise control of reaction variables, a powerful direct drive overhead stirrer and excel-lent reaction visibility even with the vacuum jacket option. Optimized for process-scale reactions from 1L to 30 L, the ReactoMate Pilot is available packaged with a Julabo A40 Presto Circulator to ensure superb tempera-ture management and unrivalled performance, a ReactoMate-Pilot Super Support system to support your jacketed reactor, and IKA overhead stirrer to ensure efficient reaction mixing. One of the problematic issues associ-ated with reaction vessel set up is the cumbersome and, often dangerous, scaffolding supporting the unit. Support frames often have bars at the front and sides this can lead to problematic access to reactor and potential frac-turing of glassware if exchanging vessels. The new ReactoMate-Pilot Super Support system provides a simple, straightforward and easy way to support your jacketed reactor up to 30 L in size. Asynt
For info: +44-(0)-1638-781709 www.asynt.com
STEM CELL FACTORS
A new range of animal-free and endotoxin-free growth factors and cytokines, tailored to stem cell research, is now available. Both expansion and differentiation of stem cells is highly reliant on growth factor and cytokine supplements, with the activity and purity of these recombinants being critical to successful research and clinical applications. Despite recognized limitations, E. coli and mammalian cells remain the dominant expression systems for commercial recombinant protein production. Although significant improvements in manufacture have been made, both systems present a risk of unwanted contamination from pathogens and endotoxins. The new range of animal-free and endotoxin-free growth factors and cytokines have been launched to address the growing body of evidence suggesting accepted levels of endotoxin contamination may significantly compromise stem cell maintenance and differentiation. These new recombinant growth factors and cytokines are produced in a plant-based expression system that utilizes barley seed to eliminate the risks and limitations associated with these traditional expression systems. AMS BiotechnologyFor info: +44-(0)-1235-828200 www.amsbio.com
REFRIGERATOR-READY DESICCATOR
The Scienceware Secador Refrigerator-Ready Desiccator maintains a low-humidity environment inside a refrigerator or -20∞C freezer. This new ad-dition to the best-selling Secador desiccator family has extralong depth to maximize refrigerator/freezer space, a slide out shelf for easy access to contents, and a fold-down door with a one-handed locking latch. Desic-cant is kept highly visible on a specially designed support at the top of the cabinet, while rugged, clear polycarbonate provides a chemical resistant, see-through body for full visualization of contents. Extrawide rubber feet prevent the desiccator from sliding and easily span any wire shelf spacing in refrigerators/freezers.Bel-Art Products
For info: 973-694-0500 www.belart.com
105www.sciencemag.org/products SCIENCE VOL 344 4 APRIL 2014
Produced by the Science��������������������� ����� New Products
Life Science Technologies
DIGITAL REFRIGERATED CIRCULATOR Compact and easy to use, the Advanced Digital Refrigerating Circulator combines intuitive touchpad operation with automatic or user-adjustable fluid optimization and ±0.01∞C temperature stability to maintain buffer solutions at optimum temperatures even under varying electrophoresis voltages. The Model AD07R-20 features a -20∞ to +200∞C working temperature range, large 3.75" LCD display, and variable speed pressure-suction pump. This versatile circulator also incorporates a wide variety of lab- and operator-friendly features, including a rotating control head that provides a clear view of the temperature display anywhere within a 180∞ viewing radius, self-storing reservoir cover, and a mold-ed composite top plate that dampens noise and remains cooler at high temperatures. Other standard features include remote temperature sensing capability as well as serial and USB connectivity. The AD07R-20 has a 7 L reservoir; other models, with reservoir sizes as large as 45 L, are also available.Polyscience
For info: 800-229-7569 www.polyscience.com
September 29 - October 1, 2014HudsonAlpha Biotechnology Campus
Huntsville, Alabama, USA
Bringing together preeminent leaders and thinkers
at the intersection of genomics and immunology
Register today at
presented by
Our Keynote Speakers:pChristophe Benoiste
Professor, Department of Microbiology and Immunobiology,HarvardMedical School
Mary Ellen ConleyFederal Express Chair of Excellence and Professor, Departmentof Pediatrics, University of Tennessee, College of Medicine, Memphis
Mark DavisInvestigator, Howard Hughes Medical Institute; Professor, Departmentof Microbiology and Immunology; Director, Institute for Immunity,Transplantation, and Infections, Stanford University School of Medicine
haig.aaas.org
Sponsored by
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Platinum Sponsors
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Interested in sponsoring Immunogenomics 2014?
Visit our website for more information.
Run one ELISA instead of multiple Western blots.
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0
0.5
1.0
1.5
2.0
2.5
PhosphorylationofERK1/2
(OD
450)
No Drug PD 0325901 PD 98059 SL 327 U0126
Colon (COLO 205)
Breast (SK-BR-3)
Cervical (HeLa)
Breast (MDA-MB-468)
Melanoma (SK-Mel-28)
Epidermal (A431)
Lung (A549)
