· Web view1747.8383 1747.8377 0.34 0 145 1 Y.TQAIDAGPATADLYADR.A 2112 27 – 43 583.6201...

Supplemental Data. Redkar et al. (2015). Plant Cell 10.1105/tpc.114.131086

ERROR TOLERANT search for Zm-SGT1 in presence of MEK2DD, SIPK and See1

MASCOT Search ResultsProtein View: gi|670432243PREDICTED: protein SGT1 homolog [Zea mays]

Database: NCBInrScore: 9381Nominal mass (Mr): 40288Calculated pI: 4.93Taxonomy: Zea mays

This protein sequence matches the following other entries:

gi|413950678 from Zea mays

Sequence similarity is available as an NCBI BLAST search of gi|670432243 against nr.

Search parameters

MS data file: \\212.87.29.88\Orbita\DO\1401-styczen\40123128krzy_AR1.raw

Enzyme: Trypsin: cuts C-term side of KR unless next residue is P.Fixed modifications: Carbamidomethyl (C)Variable modifications: Carboxymethyl (K), Oxidation (M), Phospho (ST), Phospho (Y)

Protein sequence coverage: 63%Matched peptides shown in bold red.

1 MAASDLESKA KEAFVDDDFE LAAELYTQAI DAGPATADLY ADRAQAHIKL51 GNYTEAVADA NKAIGLDPTM HKAYYRKGAA CIKLEEYQTA KAALELGSSY101 APGDSRFTRL LKECDECIAE ESSQAPAKNV EAPVAATVED KEDVANMDNT

151 PPVVEPPSKP KYRHDYYNSA TEVVLTIYAK GVPADSVVID FGDQMLSVSI201 EVPGEEPYHF QPRLFSKIIP EKCKYQVLST KVEIRLAKAE QVTWTTLDYS251 GRPKAIPQKI STPAETAPRP SYPSSKSKKD WDKLEAEVKK EEKEEKLEGD301 AALNKFFRDI YKDADEDMRR AMDKSFRESN GTVLSTNWKD VGSKTVEASP351 PDGMELKKWE I

Unformatted sequence string: 361 residues (for pasting into other applications).

Residue Number Increasing Mass Decreasing Mass

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 261 2 – 9 431.7116 861.4087 861.4192 -12.2 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 262 2 – 9 431.7117 861.4088 861.4192 -12.0 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 263 2 – 9 431.7118 861.4091 861.4192 -11.7 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 264 2 – 9 431.7119 861.4092 861.4192 -11.6 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 265 2 – 9 431.7119 861.4092 861.4192 -11.5 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 266 2 – 9 431.7120 861.4095 861.4192 -11.2 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 267 2 – 9 431.7121 861.4096 861.4192 -11.1 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 268 2 – 9 431.7122 861.4099 861.4192 -10.7 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 2938 23 – 43 742.3623 2224.0651 2224.0648 0.15 0 143 1 A.AELYTQAIDAGPATADLYADR.A 2939 23 – 43 1113.0459 2224.0772 2224.0648 5.62 0 79 1 A.AELYTQAIDAGPATADLYADR.A 2766 25 – 43 1013.0003 2023.9860 2023.9851 0.49 0 117 1 E.LYTQAIDAGPATADLYADR.A 2111 27 – 43 874.9264 1747.8383 1747.8377 0.34 0 145 1 Y.TQAIDAGPATADLYADR.A 2112 27 – 43 583.6201 1747.8386 1747.8377 0.51 0 100 1 Y.TQAIDAGPATADLYADR.A 2113 27 – 43 583.6207 1747.8402 1747.8377 1.45 0 91 1 Y.TQAIDAGPATADLYADR.A 2114 27 – 43 583.6207 1747.8402 1747.8377 1.45 0 84 1 Y.TQAIDAGPATADLYADR.A 2115 27 – 43 583.6207 1747.8403 1747.8377 1.50 0 88 1 Y.TQAIDAGPATADLYADR.A 2116 27 – 43 583.6208 1747.8407 1747.8377 1.72 0 88 1 Y.TQAIDAGPATADLYADR.A 2117 27 – 43 583.6209 1747.8409 1747.8377 1.85 0 90 1 Y.TQAIDAGPATADLYADR.A 2118 27 – 43 874.9282 1747.8418 1747.8377 2.35 0 145 1 Y.TQAIDAGPATADLYADR.A 2119 27 – 43 874.9283 1747.8421 1747.8377 2.52 0 141 1 Y.TQAIDAGPATADLYADR.A 2120 27 – 43 874.9284 1747.8421 1747.8377 2.56 0 149 1 Y.TQAIDAGPATADLYADR.A 2121 27 – 43 874.9285 1747.8425 1747.8377 2.78 0 145 1 Y.TQAIDAGPATADLYADR.A 1681 29 – 43 760.3728 1518.7310 1518.7314 -0.29 0 131 1 Q.AIDAGPATADLYADR.A 1682 29 – 43 760.3732 1518.7319 1518.7314 0.32 0 113 1 Q.AIDAGPATADLYADR.A 1683 29 – 43 507.2513 1518.7321 1518.7314 0.47 0 91 1 Q.AIDAGPATADLYADR.A 1684 29 – 43 507.2515 1518.7326 1518.7314 0.77 0 108 1 Q.AIDAGPATADLYADR.A 1685 29 – 43 507.2515 1518.7327 1518.7314 0.85 0 92 1 Q.AIDAGPATADLYADR.A 1686 29 – 43 507.2516 1518.7329 1518.7314 0.95 0 108 1 Q.AIDAGPATADLYADR.A

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Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1687 29 – 43 760.3737 1518.7329 1518.7314 0.96 0 130 1 Q.AIDAGPATADLYADR.A 1688 29 – 43 507.2516 1518.7330 1518.7314 1.03 0 96 1 Q.AIDAGPATADLYADR.A 1689 29 – 43 760.3738 1518.7331 1518.7314 1.13 0 117 1 Q.AIDAGPATADLYADR.A 1690 29 – 43 760.3739 1518.7332 1518.7314 1.15 0 130 1 Q.AIDAGPATADLYADR.A 1691 29 – 43 760.3740 1518.7334 1518.7314 1.33 0 117 1 Q.AIDAGPATADLYADR.A 1692 29 – 43 760.3740 1518.7334 1518.7314 1.33 0 121 1 Q.AIDAGPATADLYADR.A 1693 29 – 43 760.3740 1518.7334 1518.7314 1.34 0 115 1 Q.AIDAGPATADLYADR.A 1694 29 – 43 760.3740 1518.7335 1518.7314 1.36 0 130 1 Q.AIDAGPATADLYADR.A 1695 29 – 43 760.3741 1518.7335 1518.7314 1.40 0 119 1 Q.AIDAGPATADLYADR.A 1696 29 – 43 507.2519 1518.7339 1518.7314 1.62 0 92 1 Q.AIDAGPATADLYADR.A 1697 29 – 43 760.3745 1518.7345 1518.7314 2.04 0 117 1 Q.AIDAGPATADLYADR.A 1698 29 – 43 760.3746 1518.7346 1518.7314 2.13 0 124 1 Q.AIDAGPATADLYADR.A 1699 29 – 43 760.3747 1518.7349 1518.7314 2.33 0 125 1 Q.AIDAGPATADLYADR.A 1700 29 – 43 760.3750 1518.7354 1518.7314 2.62 0 125 1 Q.AIDAGPATADLYADR.A 1577 30 – 43 724.8556 1447.6966 1447.6943 1.58 0 129 1 A.IDAGPATADLYADR.A 1578 30 – 43 724.8560 1447.6975 1447.6943 2.18 0 134 1 A.IDAGPATADLYADR.A 1418 50 – 62 683.3371 1364.6596 1364.6572 1.79 0 86 2.1e-05 1 K.LGNYTEAVADANK.A 1419 50 – 62 683.3372 1364.6599 1364.6572 2.01 0 96 1.9e-06 1 K.LGNYTEAVADANK.A 1420 50 – 62 683.8288 1365.6429 1365.6605 -12.9 0 106 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1421 50 – 62 683.8289 1365.6432 1365.6605 -12.7 0 106 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1422 50 – 62 683.8291 1365.6436 1365.6605 -12.4 0 104 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1423 50 – 62 683.8291 1365.6437 1365.6605 -12.4 0 108 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1424 50 – 62 683.8295 1365.6445 1365.6605 -11.8 0 98 1 K.LGNYTEAVADANK.A + [+1.0034 at C-term K] 685 63 – 72 541.7871 1081.5596 1081.5590 0.59 0 66 0.0013 1 U K.AIGLDPTMHK.A 686 63 – 72 541.7874 1081.5603 1081.5590 1.22 0 65 0.0019 1 U K.AIGLDPTMHK.A 687 63 – 72 361.5276 1081.5609 1081.5590 1.79 0 66 0.0015 1 U K.AIGLDPTMHK.A 688 63 – 72 361.5278 1081.5615 1081.5590 2.34 0 61 0.0048 1 U K.AIGLDPTMHK.A 470 84 – 91 491.2487 980.4829 980.4814 1.53 0 56 0.02 1 K.LEEYQTAK.A 1632 92 – 106 747.3650 1492.7155 1492.7158 -0.19 0 97 1.9e-06 1 U K.AALELGSSYAPGDSR.F 1633 92 – 106 747.3657 1492.7168 1492.7158 0.68 0 90 7.8e-06 1 U K.AALELGSSYAPGDSR.F 1634 92 – 106 747.3659 1492.7173 1492.7158 1.01 0 108 1.2e-07 1 U K.AALELGSSYAPGDSR.F 1635 92 – 106 747.3681 1492.7215 1492.7158 3.88 0 90 8.8e-06 1 U K.AALELGSSYAPGDSR.F 1840 92 – 106 776.3692 1550.7239 1550.7212 1.71 0 63 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 1841 92 – 106 776.3712 1550.7279 1550.7212 4.28 0 72 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 1348 94 – 106 676.3306 1350.6467 1350.6415 3.80 0 70 1 U A.LELGSSYAPGDSR.F 1325 129 – 141 671.8473 1341.6800 1341.6776 1.77 0 93 3.1e-06 1 U K.NVEAPVAATVEDK.E 1326 129 – 141 671.8476 1341.6806 1341.6776 2.23 0 87 1.1e-05 1 U K.NVEAPVAATVEDK.E 3714 129 – 159 816.4010 3261.5749 3261.5711 1.18 1 85 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3716 129 – 159 1088.1993 3261.5761 3261.5711 1.53 1 96 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3717 129 – 159 1088.1995 3261.5767 3261.5711 1.71 1 142 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3718 129 – 159 816.4016 3261.5771 3261.5711 1.85 1 70 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3719 129 – 159 816.4016 3261.5771 3261.5711 1.85 1 78 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3732 129 – 159 1093.5333 3277.5781 3277.5660 3.68 1 90 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3798 129 – 161 1163.2436 3486.7090 3486.7188 -2.82 1 183 3.6e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3799 129 – 161 1163.2448 3486.7126 3486.7188 -1.79 1 173 3.2e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3800 129 – 161 698.3500 3486.7136 3486.7188 -1.50 1 146 1.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3802 129 – 161 698.3503 3486.7153 3486.7188 -1.02 1 140 5.8e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3803 129 – 161 698.3503 3486.7153 3486.7188 -1.02 1 156 1.7e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3805 129 – 161 698.3504 3486.7157 3486.7188 -0.90 1 163 3.5e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3807 129 – 161 872.6863 3486.7163 3486.7188 -0.72 1 131 4.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3808 129 – 161 872.6865 3486.7168 3486.7188 -0.57 1 123 3.2e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3809 129 – 161 872.6865 3486.7170 3486.7188 -0.53 1 101 4.7e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3810 129 – 161 872.6866 3486.7172 3486.7188 -0.47 1 137 1.4e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3811 129 – 161 872.6866 3486.7172 3486.7188 -0.47 1 133 3.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3812 129 – 161 872.6867 3486.7178 3486.7188 -0.30 1 159 7.9e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3813 129 – 161 872.6868 3486.7182 3486.7188 -0.17 1 182 4.1e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3817 129 – 161 872.6872 3486.7195 3486.7188 0.21 1 157 1.3e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3818 129 – 161 872.6872 3486.7196 3486.7188 0.23 1 126 1.4e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3820 129 – 161 698.3513 3486.7199 3486.7188 0.30 1 156 1.5e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3821 129 – 161 872.6873 3486.7200 3486.7188 0.33 1 134 2.4e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3822 129 – 161 698.3513 3486.7201 3486.7188 0.38 1 125 1.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3823 129 – 161 872.6873 3486.7202 3486.7188 0.40 1 138 9.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3824 129 – 161 1163.2475 3486.7207 3486.7188 0.53 1 194 2.5e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3827 129 – 161 872.6880 3486.7227 3486.7188 1.11 1 62 0.0034 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3830 129 – 161 872.6881 3486.7231 3486.7188 1.24 1 159 8e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3833 129 – 161 872.6882 3486.7238 3486.7188 1.42 1 154 2.5e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3834 129 – 161 872.6883 3486.7243 3486.7188 1.56 1 179 6.8e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3835 129 – 161 872.6884 3486.7246 3486.7188 1.66 1 166 1.4e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3836 129 – 161 698.3522 3486.7248 3486.7188 1.71 1 83 2.8e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3838 129 – 161 872.6886 3486.7255 3486.7188 1.91 1 172 3.6e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3840 129 – 161 1163.2493 3486.7261 3486.7188 2.08 1 186 1.5e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3841 129 – 161 1163.2494 3486.7264 3486.7188 2.17 1 173 2.7e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3842 129 – 161 1163.2495 3486.7267 3486.7188 2.25 1 169 7.2e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3845 129 – 161 872.6891 3486.7274 3486.7188 2.46 1 60 0.0057 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3846 129 – 161 872.6892 3486.7277 3486.7188 2.56 1 125 1.9e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3847 129 – 161 872.6893 3486.7280 3486.7188 2.64 1 139 7.3e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3848 129 – 161 872.6893 3486.7281 3486.7188 2.67 1 52 0.04 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3849 129 – 161 1163.2501 3486.7285 3486.7188 2.77 1 131 4.8e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3853 129 – 161 1163.2527 3486.7363 3486.7188 5.01 1 86 1.2e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3854 129 – 161 1163.2540 3486.7402 3486.7188 6.13 1 194 2.3e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3865 129 – 161 1168.5759 3502.7059 3502.7137 -2.24 1 180 7.1e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3867 129 – 161 1168.5767 3502.7083 3502.7137 -1.56 1 125 2.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3869 129 – 161 876.6853 3502.7119 3502.7137 -0.51 1 53 0.036 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3871 129 – 161 701.5502 3502.7144 3502.7137 0.18 1 152 4.1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3873 129 – 161 876.6862 3502.7157 3502.7137 0.55 1 126 1.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3875 129 – 161 876.6864 3502.7163 3502.7137 0.74 1 122 4.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3876 129 – 161 876.6864 3502.7164 3502.7137 0.76 1 155 2.1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3877 129 – 161 701.5506 3502.7166 3502.7137 0.81 1 156 1.7e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3878 129 – 161 701.5506 3502.7167 3502.7137 0.85 1 187 1.2e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3880 129 – 161 1168.5796 3502.7170 3502.7137 0.93 1 54 0.027 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3881 129 – 161 876.6867 3502.7177 3502.7137 1.13 1 135 2.1e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3882 129 – 161 701.5508 3502.7177 3502.7137 1.14 1 144 2.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3884 129 – 161 701.5509 3502.7180 3502.7137 1.21 1 112 3.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3886 129 – 161 1168.5803 3502.7191 3502.7137 1.53 1 146 1.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3887 129 – 161 876.6871 3502.7193 3502.7137 1.60 1 145 2.2e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3889 129 – 161 876.6872 3502.7196 3502.7137 1.68 1 113 3e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3890 129 – 161 1168.5806 3502.7200 3502.7137 1.78 1 122 3.7e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3891 129 – 161 701.5514 3502.7206 3502.7137 1.97 1 106 1.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3893 129 – 161 876.6878 3502.7220 3502.7137 2.37 1 115 2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3895 129 – 161 876.6880 3502.7229 3502.7137 2.61 1 130 5.8e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3896 129 – 161 1168.5830 3502.7272 3502.7137 3.84 1 130 6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3898 129 – 161 1168.5850 3502.7332 3502.7137 5.55 1 144 2.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3540 137 – 161 684.8397 2735.3297 2735.3324 -0.99 1 58 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3541 137 – 161 684.8401 2735.3312 2735.3324 -0.42 1 69 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3542 137 – 161 912.7849 2735.3327 2735.3324 0.13 1 132 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3543 137 – 161 684.8405 2735.3329 2735.3324 0.21 1 113 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3544 137 – 161 684.8406 2735.3333 2735.3324 0.33 1 104 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3545 137 – 161 684.8408 2735.3341 2735.3324 0.63 1 106 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3546 137 – 161 912.7855 2735.3348 2735.3324 0.90 1 78 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3547 137 – 161 912.7857 2735.3353 2735.3324 1.06 1 120 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3549 137 – 161 684.8413 2735.3361 2735.3324 1.36 1 80 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3567 137 – 161 688.8397 2751.3295 2751.3273 0.82 1 62 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3568 137 – 161 688.8399 2751.3307 2751.3273 1.22 1 69 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3442 138 – 161 659.5792 2634.2877 2634.2847 1.16 1 115 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3443 138 – 161 659.5795 2634.2888 2634.2847 1.55 1 90 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3444 138 – 161 659.5797 2634.2897 2634.2847 1.92 1 74 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3445 138 – 161 879.1045 2634.2916 2634.2847 2.61 1 103 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3446 138 – 161 659.5804 2634.2924 2634.2847 2.94 1 121 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3447 138 – 161 879.1048 2634.2925 2634.2847 2.95 1 120 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3464 138 – 161 663.5781 2650.2832 2650.2796 1.36 1 61 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 2902 142 – 161 722.0256 2163.0549 2163.0518 1.42 0 129 1.1e-09 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2597 197 – 213 657.6596 1969.9569 1969.9534 1.78 0 59 1 L.SVSIEVPGEEPYHFQPR.L 2032 200 – 213 566.6151 1696.8235 1696.8209 1.55 0 104 1 S.IEVPGEEPYHFQPR.L 2033 200 – 213 566.6155 1696.8246 1696.8209 2.17 0 98 1 S.IEVPGEEPYHFQPR.L 2034 200 – 213 566.6173 1696.8300 1696.8209 5.33 0 98 1 S.IEVPGEEPYHFQPR.L 201 225 – 231 419.7379 837.4612 837.4596 1.96 0 53 0.015 1 K.YQVLSTK.V 1974 239 – 252 542.9303 1625.7690 1625.7686 0.24 0 71 1 K.AEQVTWTTLDYSGR.P 1975 239 – 252 813.8918 1625.7691 1625.7686 0.34 0 103 1 K.AEQVTWTTLDYSGR.P 1976 239 – 252 813.8918 1625.7691 1625.7686 0.34 0 97 1 K.AEQVTWTTLDYSGR.P 1977 239 – 252 542.9303 1625.7692 1625.7686 0.37 0 65 1 K.AEQVTWTTLDYSGR.P 1978 239 – 252 542.9304 1625.7695 1625.7686 0.58 0 68 1 K.AEQVTWTTLDYSGR.P 2279 239 – 254 926.4657 1850.9168 1850.9163 0.29 0 92 5.2e-06 1 K.AEQVTWTTLDYSGRPK.A 2280 239 – 254 926.4666 1850.9186 1850.9163 1.25 0 100 7.7e-07 1 K.AEQVTWTTLDYSGRPK.A 2281 239 – 254 926.4668 1850.9190 1850.9163 1.45 0 96 2e-06 1 K.AEQVTWTTLDYSGRPK.A 2212 260 – 276 606.9800 1817.9180 1817.9159 1.16 0 60 1 K.ISTPAETAPRPSYPSSK.S + [+30.0106 at A5] 2213 260 – 276 606.9802 1817.9188 1817.9159 1.59 0 58 1 K.ISTPAETAPRPSYPSSK.S + [+30.0106 at A5] 2214 260 – 276 606.9807 1817.9203 1817.9159 2.42 0 60 1 K.ISTPAETAPRPSYPSSK.S + [+30.0106 at A5] 2321 260 – 276 633.6356 1897.8849 1897.8822 1.41 0 97 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2322 260 – 276 949.9498 1897.8851 1897.8822 1.49 0 128 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2326 260 – 276 633.6358 1897.8856 1897.8822 1.79 0 101 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2327 260 – 276 633.6359 1897.8857 1897.8822 1.84 0 87 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2329 260 – 276 949.9504 1897.8862 1897.8822 2.10 0 93 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2332 260 – 276 949.9514 1897.8882 1897.8822 3.13 0 117 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 67 270 – 276 383.1933 764.3720 764.3705 2.06 0 52 1 R.PSYPSSK.S 69 270 – 276 383.1934 764.3723 764.3705 2.40 0 52 1 R.PSYPSSK.S 70 270 – 276 383.1938 764.3730 764.3705 3.29 0 52 1 R.PSYPSSK.S 1380 279 – 289 340.9329 1359.7025 1359.6882 10.5 2 51 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0207 at W3] 1386 279 – 289 454.2418 1359.7036 1359.7034 0.12 2 58 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1415 279 – 289 454.5774 1360.7104 1360.7068 2.70 2 75 1 K.KDWDKLEAEVK.K + [+1.0034 at N-term K] 995 280 – 289 616.8109 1231.6072 1231.6084 -0.97 1 69 0.001 5 K.DWDKLEAEVK.K 997 280 – 289 616.8111 1231.6077 1231.6084 -0.58 1 64 0.0034 5 K.DWDKLEAEVK.K 998 280 – 289 411.5433 1231.6082 1231.6084 -0.23 1 68 0.0014 4 K.DWDKLEAEVK.K 999 280 – 289 616.8114 1231.6082 1231.6084 -0.22 1 69 0.00096 5 K.DWDKLEAEVK.K 1002 280 – 289 616.8116 1231.6087 1231.6084 0.18 1 71 0.00059 5 K.DWDKLEAEVK.K 1003 280 – 289 411.5435 1231.6088 1231.6084 0.26 1 68 0.0012 4 K.DWDKLEAEVK.K 1006 280 – 289 616.8118 1231.6091 1231.6084 0.56 1 58 0.014 5 K.DWDKLEAEVK.K 1007 280 – 289 411.5437 1231.6092 1231.6084 0.62 1 53 0.044 6 K.DWDKLEAEVK.K 1008 280 – 289 616.8119 1231.6093 1231.6084 0.72 1 55 0.027 5 K.DWDKLEAEVK.K 1011 280 – 289 411.5438 1231.6095 1231.6084 0.82 1 68 0.0013 4 K.DWDKLEAEVK.K 1012 280 – 289 616.8120 1231.6095 1231.6084 0.83 1 65 0.0028 4 K.DWDKLEAEVK.K 1013 280 – 289 411.5438 1231.6095 1231.6084 0.84 1 51 0.061 4 K.DWDKLEAEVK.K 1014 280 – 289 411.5438 1231.6095 1231.6084 0.89 1 67 0.0017 4 K.DWDKLEAEVK.K 1015 280 – 289 616.8120 1231.6095 1231.6084 0.90 1 60 0.0087 5 K.DWDKLEAEVK.K 1017 280 – 289 616.8121 1231.6096 1231.6084 0.93 1 54 0.031 4 K.DWDKLEAEVK.K 1019 280 – 289 411.5438 1231.6096 1231.6084 0.94 1 57 0.017 4 K.DWDKLEAEVK.K 1020 280 – 289 616.8121 1231.6096 1231.6084 0.96 1 56 0.021 5 K.DWDKLEAEVK.K 1022 280 – 289 411.5438 1231.6096 1231.6084 0.96 1 52 0.052 1 K.DWDKLEAEVK.K 1023 280 – 289 411.5438 1231.6097 1231.6084 1.01 1 56 0.021 5 K.DWDKLEAEVK.K 1024 280 – 289 411.5438 1231.6097 1231.6084 1.01 1 62 0.0054 4 K.DWDKLEAEVK.K 1025 280 – 289 411.5439 1231.6097 1231.6084 1.04 1 55 0.026 1 K.DWDKLEAEVK.K 1027 280 – 289 411.5439 1231.6100 1231.6084 1.23 1 59 0.011 1 K.DWDKLEAEVK.K 1028 280 – 289 411.5439 1231.6100 1231.6084 1.23 1 59 0.0094 1 K.DWDKLEAEVK.K 1029 280 – 289 616.8123 1231.6100 1231.6084 1.30 1 55 0.025 4 K.DWDKLEAEVK.K 1030 280 – 289 411.5440 1231.6102 1231.6084 1.40 1 72 0.00056 1 K.DWDKLEAEVK.K 1036 280 – 289 616.8124 1231.6103 1231.6084 1.48 1 62 0.0053 5 K.DWDKLEAEVK.K 1037 280 – 289 616.8124 1231.6103 1231.6084 1.53 1 57 0.016 1 K.DWDKLEAEVK.K 1038 280 – 289 616.8125 1231.6104 1231.6084 1.56 1 67 0.0017 5 K.DWDKLEAEVK.K 1041 280 – 289 411.5441 1231.6104 1231.6084 1.62 1 55 0.028 1 K.DWDKLEAEVK.K 1042 280 – 289 411.5441 1231.6104 1231.6084 1.62 1 52 0.049 1 K.DWDKLEAEVK.K 1044 280 – 289 411.5442 1231.6106 1231.6084 1.77 1 55 0.028 4 K.DWDKLEAEVK.K 1045 280 – 289 616.8126 1231.6106 1231.6084 1.77 1 61 0.0069 4 K.DWDKLEAEVK.K 1047 280 – 289 616.8126 1231.6106 1231.6084 1.79 1 67 0.0018 4 K.DWDKLEAEVK.K 1049 280 – 289 411.5442 1231.6107 1231.6084 1.82 1 67 0.0016 4 K.DWDKLEAEVK.K 1052 280 – 289 411.5442 1231.6108 1231.6084 1.94 1 67 0.0015 4 K.DWDKLEAEVK.K 1058 280 – 289 411.5444 1231.6113 1231.6084 2.35 1 69 0.00098 4 K.DWDKLEAEVK.K 1083 280 – 289 416.8753 1247.6040 1247.6034 0.47 1 66 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1084 280 – 289 624.8094 1247.6042 1247.6034 0.65 1 65 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1085 280 – 289 624.8095 1247.6045 1247.6034 0.90 1 72 1 K.DWDKLEAEVK.K + [+15.9949 at W2]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1086 280 – 289 624.8095 1247.6045 1247.6034 0.95 1 53 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1088 280 – 289 416.8756 1247.6049 1247.6034 1.22 1 59 2 K.DWDKLEAEVK.K + [+15.9949 at W2] 1089 280 – 289 416.8757 1247.6054 1247.6034 1.63 1 55 4 K.DWDKLEAEVK.K + [+15.9949 at W2] 1091 280 – 289 624.8101 1247.6056 1247.6034 1.82 1 51 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1128 280 – 289 422.2075 1263.6006 1263.5983 1.88 1 55 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1404 280 – 289 454.2430 1359.7071 1359.7034 2.69 1 67 1 K.DWDKLEAEVK.K + [+128.0950 at C-term K] 2042 291 – 305 568.2882 1701.8428 1701.8421 0.44 2 89 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2047 291 – 305 568.2886 1701.8441 1701.8421 1.21 2 80 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2202 291 – 305 604.6400 1810.8983 1810.8886 5.36 2 73 2 K.EEKEEKLEGDAALNK.F + [+109.0466 at N-term] 2199 291 – 305 604.6392 1810.8959 1810.8948 0.62 2 51 1 K.EEKEEKLEGDAALNK.F + [+109.0528 at K6] 2201 291 – 305 604.6397 1810.8974 1810.8948 1.41 2 66 1 K.EEKEEKLEGDAALNK.F + [+109.0528 at K6] 2200 291 – 305 604.6397 1810.8973 1810.9510 -29.7 2 82 2 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); 3 [+17.0345 at E1,E2,E4] 2203 291 – 305 604.6403 1810.8990 1810.9510 -28.7 2 59 2 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); 3 [+17.0345 at E1,E2,E4] 2232 291 – 305 610.9861 1829.9364 1829.9370 -0.30 2 78 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2236 291 – 305 610.9867 1829.9381 1829.9370 0.62 2 118 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2238 291 – 305 458.4919 1829.9386 1829.9370 0.86 2 87 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2239 291 – 305 610.9869 1829.9388 1829.9370 1.01 2 89 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2243 291 – 305 610.9870 1829.9393 1829.9370 1.27 2 99 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2244 291 – 305 610.9872 1829.9398 1829.9370 1.53 2 104 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 1235 294 – 305 439.5612 1315.6617 1315.6619 -0.20 1 56 0.017 1 K.EEKLEGDAALNK.F 1237 294 – 305 439.5615 1315.6627 1315.6619 0.60 1 57 0.012 1 K.EEKLEGDAALNK.F 1238 294 – 305 439.5615 1315.6628 1315.6619 0.65 1 80 7e-05 1 K.EEKLEGDAALNK.F 1239 294 – 305 658.8388 1315.6631 1315.6619 0.89 1 76 0.00017 1 K.EEKLEGDAALNK.F 1242 294 – 305 439.5617 1315.6633 1315.6619 1.06 1 90 6.3e-06 1 K.EEKLEGDAALNK.F 1243 294 – 305 439.5618 1315.6637 1315.6619 1.33 1 79 8e-05 1 K.EEKLEGDAALNK.F 1246 294 – 305 658.8395 1315.6644 1315.6619 1.90 1 83 3.2e-05 1 K.EEKLEGDAALNK.F 1248 294 – 305 658.8397 1315.6649 1315.6619 2.28 1 74 0.00028 1 K.EEKLEGDAALNK.F 1249 294 – 305 658.8398 1315.6651 1315.6619 2.45 1 75 0.00019 1 K.EEKLEGDAALNK.F 1250 294 – 305 658.8401 1315.6655 1315.6619 2.76 1 84 2.8e-05 1 K.EEKLEGDAALNK.F 401 297 – 305 465.7483 929.4820 929.4818 0.27 0 72 0.00047 1 K.LEGDAALNK.F 402 297 – 305 465.7489 929.4831 929.4818 1.47 0 77 0.00013 1 K.LEGDAALNK.F 403 297 – 305 465.7491 929.4835 929.4818 1.90 0 75 0.00023 1 K.LEGDAALNK.F 1410 297 – 308 681.3469 1360.6793 1360.7099 -22.5 1 74 1 K.LEGDAALNKFFR.D + [-19.0098 at F10] 1430 309 – 319 457.5347 1369.5824 1369.5820 0.29 1 55 0.029 1 R.DIYKDADEDMR.R 1431 309 – 319 685.7986 1369.5826 1369.5820 0.45 1 78 0.00014 1 R.DIYKDADEDMR.R 1432 309 – 319 457.5349 1369.5828 1369.5820 0.61 1 63 0.0048 1 R.DIYKDADEDMR.R 1434 309 – 319 457.5351 1369.5835 1369.5820 1.10 1 88 1.5e-05 1 R.DIYKDADEDMR.R 1435 309 – 319 457.5351 1369.5835 1369.5820 1.12 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1436 309 – 319 685.7991 1369.5836 1369.5820 1.18 1 93 4.3e-06 1 R.DIYKDADEDMR.R 1437 309 – 319 685.7991 1369.5837 1369.5820 1.24 1 93 4.2e-06 1 R.DIYKDADEDMR.R 1438 309 – 319 685.7992 1369.5838 1369.5820 1.33 1 67 0.0019 1 R.DIYKDADEDMR.R 1439 309 – 319 457.5352 1369.5838 1369.5820 1.34 1 55 0.027 1 R.DIYKDADEDMR.R 1440 309 – 319 685.7992 1369.5839 1369.5820 1.39 1 103 4.3e-07 1 R.DIYKDADEDMR.R 1441 309 – 319 685.7993 1369.5839 1369.5820 1.44 1 103 4.3e-07 1 R.DIYKDADEDMR.R 1442 309 – 319 685.7993 1369.5840 1369.5820 1.47 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1443 309 – 319 457.5353 1369.5841 1369.5820 1.58 1 86 2.1e-05 1 R.DIYKDADEDMR.R 1444 309 – 319 457.5355 1369.5847 1369.5820 2.02 1 88 1.3e-05 1 R.DIYKDADEDMR.R

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1445 309 – 319 457.5356 1369.5850 1369.5820 2.21 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1446 309 – 319 457.5356 1369.5851 1369.5820 2.26 1 82 5.1e-05 1 R.DIYKDADEDMR.R 1447 309 – 319 457.5356 1369.5851 1369.5820 2.28 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1448 309 – 319 457.5356 1369.5851 1369.5820 2.30 1 82 5.3e-05 1 R.DIYKDADEDMR.R 1476 309 – 319 462.8665 1385.5776 1385.5769 0.50 1 78 0.00011 1 R.DIYKDADEDMR.R + Oxidation (M) 1477 309 – 319 693.7962 1385.5779 1385.5769 0.72 1 69 0.00088 1 R.DIYKDADEDMR.R + Oxidation (M) 1478 309 – 319 693.7967 1385.5788 1385.5769 1.37 1 54 0.029 1 R.DIYKDADEDMR.R + Oxidation (M) 1479 309 – 319 462.8669 1385.5788 1385.5769 1.39 1 82 4.3e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1481 309 – 319 693.7969 1385.5792 1385.5769 1.66 1 64 0.003 1 R.DIYKDADEDMR.R + Oxidation (M) 1526 309 – 319 471.2108 1410.6106 1410.6085 1.50 1 63 1 R.DIYKDADEDMR.R + [+41.0265 at K4] 1553 309 – 319 479.5017 1435.4832 1435.4963 -9.08 1 55 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1554 309 – 319 718.7492 1435.4838 1435.4963 -8.66 1 57 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1555 309 – 319 718.7494 1435.4843 1435.4963 -8.32 1 62 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 233 313 – 319 426.1641 850.3137 850.3127 1.18 0 59 0.0046 1 K.DADEDMR.R 234 313 – 319 426.1648 850.3150 850.3127 2.76 0 62 0.0023 1 K.DADEDMR.R 236 313 – 319 426.1652 850.3158 850.3127 3.68 0 62 0.0023 1 K.DADEDMR.R 1298 328 – 339 668.3316 1334.6486 1334.6466 1.46 0 62 0.0044 1 U R.ESNGTVLSTNWK.D 1299 328 – 339 668.3317 1334.6488 1334.6466 1.63 0 73 0.00038 1 U R.ESNGTVLSTNWK.D 1300 328 – 339 668.3317 1334.6488 1334.6466 1.63 0 62 0.0043 1 U R.ESNGTVLSTNWK.D 1301 328 – 339 668.3319 1334.6493 1334.6466 2.02 0 73 0.00037 1 U R.ESNGTVLSTNWK.D 1302 328 – 339 668.8208 1335.6271 1335.6500 -17.1 0 72 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1303 328 – 339 668.8216 1335.6285 1335.6500 -16.1 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1304 328 – 339 668.8221 1335.6296 1335.6500 -15.2 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1305 328 – 339 668.8228 1335.6310 1335.6500 -14.2 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1451 345 – 357 687.3349 1372.6553 1372.6544 0.63 0 88 1.2e-05 1 U K.TVEASPPDGMELK.K 1452 345 – 357 687.3351 1372.6556 1372.6544 0.87 0 100 7.2e-07 1 U K.TVEASPPDGMELK.K 1453 345 – 357 687.3351 1372.6556 1372.6544 0.90 0 113 4.5e-08 1 U K.TVEASPPDGMELK.K 1454 345 – 357 687.3352 1372.6559 1372.6544 1.10 0 91 6.2e-06 1 U K.TVEASPPDGMELK.K 1455 345 – 357 687.3356 1372.6566 1372.6544 1.59 0 91 6.7e-06 1 U K.TVEASPPDGMELK.K 1456 345 – 357 687.3356 1372.6566 1372.6544 1.62 0 99 1.1e-06 1 U K.TVEASPPDGMELK.K 1457 345 – 357 687.3357 1372.6569 1372.6544 1.81 0 110 8.6e-08 1 U K.TVEASPPDGMELK.K 1458 345 – 357 687.3358 1372.6570 1372.6544 1.86 0 105 2.3e-07 1 U K.TVEASPPDGMELK.K 1460 345 – 357 687.3359 1372.6572 1372.6544 2.00 0 105 2.7e-07 1 U K.TVEASPPDGMELK.K 1461 345 – 357 687.3359 1372.6573 1372.6544 2.08 0 92 5.1e-06 1 U K.TVEASPPDGMELK.K 1462 345 – 357 687.3365 1372.6584 1372.6544 2.89 0 93 4.1e-06 1 U K.TVEASPPDGMELK.K 1463 345 – 357 687.3369 1372.6593 1372.6544 3.56 0 71 0.00058 1 U K.TVEASPPDGMELK.K 1486 345 – 357 695.3320 1388.6495 1388.6493 0.15 0 83 3.9e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1488 345 – 357 695.3328 1388.6511 1388.6493 1.27 0 80 7.7e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1489 345 – 357 695.3329 1388.6512 1388.6493 1.32 0 92 5.7e-06 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1491 345 – 357 695.3329 1388.6513 1388.6493 1.40 0 71 0.00072 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1492 345 – 357 695.3330 1388.6514 1388.6493 1.46 0 77 0.00016 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1493 345 – 357 695.3330 1388.6514 1388.6493 1.52 0 70 0.00086 1 U K.TVEASPPDGMELK.K + Oxidation (M)

1) ERROR TOLERANT search for ZmSGT1 in presence of MEK2DD and SIPK and in absence of See1:

MASCOT Search ResultsProtein View: gi|670432243PREDICTED: protein SGT1 homolog [Zea mays]





Search parameters




1 MAASDLESKA KEAFVDDDFE LAAELYTQAI DAGPATADLY ADRAQAHIKL51 GNYTEAVADA NKAIGLDPTM HKAYYRKGAA CIKLEEYQTA KAALELGSSY101 APGDSRFTRL LKECDECIAE ESSQAPAKNV EAPVAATVED KEDVANMDNT151 PPVVEPPSKP KYRHDYYNSA TEVVLTIYAK GVPADSVVID FGDQMLSVSI201 EVPGEEPYHF QPRLFSKIIP EKCKYQVLST KVEIRLAKAE QVTWTTLDYS251 GRPKAIPQKI STPAETAPRP SYPSSKSKKD WDKLEAEVKK EEKEEKLEGD301 AALNKFFRDI YKDADEDMRR AMDKSFRESN GTVLSTNWKD VGSKTVEASP351 PDGMELKKWE I



Sort peptides by

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 270 2 – 9 431.7120 861.4094 861.4192 -11.3 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 271 2 – 9 431.7120 861.4094 861.4192 -11.3 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 272 2 – 9 431.7120 861.4095 861.4192 -11.2 0 71 1 M.AASDLESK.A + [+42.0218 at N-term] 274 2 – 9 431.7122 861.4099 861.4192 -10.7 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 275 2 – 9 431.7123 861.4100 861.4192 -10.7 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 276 2 – 9 431.7124 861.4102 861.4192 -10.4 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 277 2 – 9 431.7125 861.4105 861.4192 -10.1 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 278 2 – 9 431.7125 861.4105 861.4192 -10.0 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 44 3 – 9 375.1876 748.3606 748.3603 0.42 0 62 1 A.ASDLESK.A 45 3 – 9 375.1878 748.3610 748.3603 1.03 0 52 1 A.ASDLESK.A 46 3 – 9 375.1878 748.3610 748.3603 1.03 0 54 1 A.ASDLESK.A 47 3 – 9 375.1879 748.3613 748.3603 1.33 0 52 1 A.ASDLESK.A 2507 12 – 26 873.8899 1745.7652 1745.7672 -1.15 0 57 1 K.EAFVDDDFELAAELY.T 2853 12 – 28 988.4438 1974.8731 1974.8735 -0.18 0 55 1 K.EAFVDDDFELAAELYTQ.A 3021 23 – 43 1113.0403 2224.0660 2224.0648 0.58 0 56 1 A.AELYTQAIDAGPATADLYADR.A 3022 23 – 43 742.3641 2224.0706 2224.0648 2.61 0 121 1 A.AELYTQAIDAGPATADLYADR.A 3023 23 – 43 742.3644 2224.0714 2224.0648 2.99 0 101 1 A.AELYTQAIDAGPATADLYADR.A 3024 23 – 43 742.3650 2224.0731 2224.0648 3.73 0 97 1 A.AELYTQAIDAGPATADLYADR.A 3026 23 – 43 1113.0440 2224.0734 2224.0648 3.91 0 66 1 A.AELYTQAIDAGPATADLYADR.A 2901 25 – 43 1012.9989 2023.9832 2023.9851 -0.90 0 60 1 E.LYTQAIDAGPATADLYADR.A 2902 25 – 43 675.6691 2023.9856 2023.9851 0.25 0 62 1 E.LYTQAIDAGPATADLYADR.A 2523 27 – 43 583.6205 1747.8398 1747.8377 1.19 0 85 1 Y.TQAIDAGPATADLYADR.A 2524 27 – 43 583.6206 1747.8399 1747.8377 1.30 0 83 1 Y.TQAIDAGPATADLYADR.A 2525 27 – 43 583.6206 1747.8400 1747.8377 1.35 0 96 1 Y.TQAIDAGPATADLYADR.A 2526 27 – 43 583.6207 1747.8404 1747.8377 1.54 0 88 1 Y.TQAIDAGPATADLYADR.A 2527 27 – 43 583.6208 1747.8405 1747.8377 1.59 0 84 1 Y.TQAIDAGPATADLYADR.A 2528 27 – 43 874.9282 1747.8419 1747.8377 2.41 0 155 1 Y.TQAIDAGPATADLYADR.A 2529 27 – 43 583.6213 1747.8422 1747.8377 2.58 0 99 1 Y.TQAIDAGPATADLYADR.A 2530 27 – 43 874.9284 1747.8423 1747.8377 2.67 0 146 1 Y.TQAIDAGPATADLYADR.A 2531 27 – 43 874.9287 1747.8428 1747.8377 2.92 0 145 1 Y.TQAIDAGPATADLYADR.A 2532 27 – 43 874.9289 1747.8432 1747.8377 3.15 0 150 1 Y.TQAIDAGPATADLYADR.A 2533 27 – 43 874.9290 1747.8434 1747.8377 3.30 0 141 1 Y.TQAIDAGPATADLYADR.A 2534 27 – 43 874.9292 1747.8437 1747.8377 3.47 0 145 1 Y.TQAIDAGPATADLYADR.A 2387 28 – 43 824.4054 1646.7963 1646.7900 3.85 0 111 1 T.QAIDAGPATADLYADR.A 2122 29 – 43 760.3725 1518.7304 1518.7314 -0.64 0 116 1 Q.AIDAGPATADLYADR.A 2123 29 – 43 760.3727 1518.7309 1518.7314 -0.32 0 121 1 Q.AIDAGPATADLYADR.A 2124 29 – 43 760.3735 1518.7325 1518.7314 0.73 0 117 1 Q.AIDAGPATADLYADR.A 2125 29 – 43 507.2515 1518.7327 1518.7314 0.87 0 80 1 Q.AIDAGPATADLYADR.A 2126 29 – 43 760.3737 1518.7328 1518.7314 0.90 0 116 1 Q.AIDAGPATADLYADR.A 2127 29 – 43 760.3740 1518.7334 1518.7314 1.34 0 100 1 Q.AIDAGPATADLYADR.A 2128 29 – 43 507.2518 1518.7335 1518.7314 1.34 0 91 1 Q.AIDAGPATADLYADR.A 2129 29 – 43 760.3741 1518.7336 1518.7314 1.42 0 130 1 Q.AIDAGPATADLYADR.A

Show predicted peptides also

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2130 29 – 43 760.3741 1518.7337 1518.7314 1.48 0 108 1 Q.AIDAGPATADLYADR.A 2131 29 – 43 507.2518 1518.7337 1518.7314 1.50 0 89 1 Q.AIDAGPATADLYADR.A 2132 29 – 43 760.3744 1518.7343 1518.7314 1.90 0 130 1 Q.AIDAGPATADLYADR.A 2133 29 – 43 507.2521 1518.7345 1518.7314 2.05 0 84 1 Q.AIDAGPATADLYADR.A 2134 29 – 43 760.3746 1518.7346 1518.7314 2.09 0 130 1 Q.AIDAGPATADLYADR.A 2135 29 – 43 760.3750 1518.7353 1518.7314 2.59 0 121 1 Q.AIDAGPATADLYADR.A 2136 29 – 43 760.3751 1518.7356 1518.7314 2.73 0 122 1 Q.AIDAGPATADLYADR.A 2013 30 – 43 724.8549 1447.6953 1447.6943 0.67 0 129 1 A.IDAGPATADLYADR.A 1764 50 – 62 683.3366 1364.6586 1364.6572 1.03 0 100 7.7e-07 1 K.LGNYTEAVADANK.A 1765 50 – 62 683.3366 1364.6586 1364.6572 1.03 0 90 8.5e-06 1 K.LGNYTEAVADANK.A 1766 50 – 62 683.3370 1364.6595 1364.6572 1.73 0 72 0.00055 1 K.LGNYTEAVADANK.A 1767 50 – 62 683.3378 1364.6610 1364.6572 2.80 0 81 5.9e-05 1 K.LGNYTEAVADANK.A 1768 50 – 62 683.8279 1365.6413 1365.6605 -14.1 0 113 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1769 50 – 62 683.8288 1365.6430 1365.6605 -12.9 0 113 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1770 50 – 62 683.8289 1365.6433 1365.6605 -12.6 0 90 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1771 50 – 62 683.8293 1365.6441 1365.6605 -12.0 0 110 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1772 50 – 62 683.8298 1365.6451 1365.6605 -11.3 0 101 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1875 50 – 62 696.3453 1390.6761 1390.6728 2.36 0 61 1 K.LGNYTEAVADANK.A + [+26.0156 at N-term] 1938 50 – 62 712.8409 1423.6673 1423.6935 -18.3 0 61 10 K.LGNYTEAVADANK.A + [+59.0363 at N-term] 718 63 – 72 541.7869 1081.5593 1081.5590 0.24 0 68 0.00095 1 U K.AIGLDPTMHK.A 719 63 – 72 361.5276 1081.5609 1081.5590 1.79 0 66 0.0016 1 U K.AIGLDPTMHK.A 481 84 – 91 491.2492 980.4838 980.4814 2.43 0 53 0.034 1 K.LEEYQTAK.A 482 84 – 91 491.2493 980.4840 980.4814 2.65 0 52 0.045 1 K.LEEYQTAK.A 523 84 – 91 499.2460 996.4775 996.4764 1.13 0 52 1 K.LEEYQTAK.A + [+15.9949 at Y4] 2078 92 – 106 747.3642 1492.7138 1492.7158 -1.32 0 95 2.7e-06 1 U K.AALELGSSYAPGDSR.F 2079 92 – 106 747.3644 1492.7142 1492.7158 -1.04 0 104 3.2e-07 1 U K.AALELGSSYAPGDSR.F 2080 92 – 106 498.5788 1492.7146 1492.7158 -0.78 0 111 7.1e-08 1 U K.AALELGSSYAPGDSR.F 2081 92 – 106 498.5789 1492.7148 1492.7158 -0.61 0 99 1.1e-06 1 U K.AALELGSSYAPGDSR.F 2082 92 – 106 498.5791 1492.7156 1492.7158 -0.11 0 106 2.4e-07 1 U K.AALELGSSYAPGDSR.F 2083 92 – 106 747.3652 1492.7159 1492.7158 0.13 0 91 7e-06 1 U K.AALELGSSYAPGDSR.F 2085 92 – 106 747.3661 1492.7176 1492.7158 1.21 0 75 0.00024 1 U K.AALELGSSYAPGDSR.F 2086 92 – 106 747.3677 1492.7209 1492.7158 3.42 0 80 8.2e-05 1 U K.AALELGSSYAPGDSR.F 2087 92 – 106 747.3684 1492.7222 1492.7158 4.31 0 65 0.0026 1 U K.AALELGSSYAPGDSR.F 2088 92 – 106 747.8673 1493.7201 1493.7128 4.87 0 64 1 U K.AALELGSSYAPGDSR.F + [+0.9970 at N-term A] 2110 92 – 106 505.9028 1514.6865 1514.6977 -7.37 0 73 3 U K.AALELGSSYAPGDSR.F + [+21.9819 at E4] 2111 92 – 106 505.9031 1514.6876 1514.6977 -6.66 0 68 7 U K.AALELGSSYAPGDSR.F + [+21.9819 at E4] 2255 92 – 106 776.3680 1550.7214 1550.7212 0.14 0 108 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 2256 92 – 106 776.3683 1550.7221 1550.7212 0.56 0 115 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 2257 92 – 106 776.3691 1550.7236 1550.7212 1.50 0 91 2 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 2258 92 – 106 776.3691 1550.7236 1550.7212 1.53 0 105 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 1584 129 – 141 671.8459 1341.6773 1341.6776 -0.25 0 93 3.3e-06 1 U K.NVEAPVAATVEDK.E 1585 129 – 141 671.8469 1341.6793 1341.6776 1.25 0 91 4.4e-06 1 U K.NVEAPVAATVEDK.E 1586 129 – 141 671.8470 1341.6795 1341.6776 1.41 0 90 6e-06 1 U K.NVEAPVAATVEDK.E 1587 129 – 141 671.8472 1341.6798 1341.6776 1.65 0 91 5.1e-06 1 U K.NVEAPVAATVEDK.E 1588 129 – 141 671.8474 1341.6803 1341.6776 2.04 0 93 3.3e-06 1 U K.NVEAPVAATVEDK.E 1593 129 – 141 672.3399 1342.6652 1342.6746 -7.05 0 92 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2] 1594 129 – 141 672.3399 1342.6653 1342.6746 -6.96 0 99 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1595 129 – 141 672.3403 1342.6661 1342.6746 -6.32 0 86 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2] 1592 129 – 141 672.3394 1342.6642 1342.6810 -12.5 0 94 1 U K.NVEAPVAATVEDK.E + [+1.0034 at N-term N] 2323 129 – 143 793.8779 1585.7412 1585.7471 -3.74 1 54 1 U K.NVEAPVAATVEDKED.V 3476 129 – 159 653.3212 3261.5697 3261.5711 -0.44 1 65 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3477 129 – 159 1088.1972 3261.5698 3261.5711 -0.40 1 56 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3478 129 – 159 1088.1976 3261.5710 3261.5711 -0.036 1 164 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3479 129 – 159 1088.1985 3261.5737 3261.5711 0.79 1 136 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3480 129 – 159 816.4010 3261.5751 3261.5711 1.22 1 59 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3481 129 – 159 816.4011 3261.5751 3261.5711 1.24 1 76 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3482 129 – 159 816.4011 3261.5751 3261.5711 1.24 1 90 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3483 129 – 159 816.4012 3261.5759 3261.5711 1.46 1 95 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3486 129 – 159 816.4019 3261.5784 3261.5711 2.23 1 54 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3487 129 – 159 1088.2002 3261.5788 3261.5711 2.36 1 84 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3488 129 – 159 1088.2002 3261.5788 3261.5711 2.36 1 164 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3489 129 – 159 1088.2009 3261.5809 3261.5711 3.00 1 142 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3501 129 – 159 1093.5324 3277.5754 3277.5660 2.86 1 142 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3502 129 – 159 820.4012 3277.5759 3277.5660 3.00 1 87 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3504 129 – 159 1093.5338 3277.5796 3277.5660 4.14 1 188 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3576 129 – 161 698.3496 3486.7116 3486.7188 -2.08 1 162 4.3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3578 129 – 161 698.3501 3486.7143 3486.7188 -1.29 1 149 7.3e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3579 129 – 161 698.3502 3486.7144 3486.7188 -1.26 1 58 0.011 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3580 129 – 161 698.3503 3486.7150 3486.7188 -1.09 1 153 3.2e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3583 129 – 161 872.6863 3486.7159 3486.7188 -0.82 1 138 9.9e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3587 129 – 161 872.6865 3486.7169 3486.7188 -0.54 1 140 5.8e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3589 129 – 161 1163.2463 3486.7171 3486.7188 -0.50 1 108 1e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3590 129 – 161 872.6866 3486.7171 3486.7188 -0.49 1 134 2.3e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3591 129 – 161 872.6866 3486.7173 3486.7188 -0.43 1 105 1.9e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3592 129 – 161 872.6866 3486.7173 3486.7188 -0.43 1 132 3.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3593 129 – 161 698.3507 3486.7173 3486.7188 -0.43 1 146 1.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3594 129 – 161 1163.2464 3486.7174 3486.7188 -0.41 1 72 0.00041 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3595 129 – 161 872.6867 3486.7175 3486.7188 -0.37 1 118 1.1e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3597 129 – 161 872.6867 3486.7177 3486.7188 -0.32 1 147 1.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3598 129 – 161 698.3508 3486.7178 3486.7188 -0.30 1 137 1.2e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3601 129 – 161 872.6868 3486.7182 3486.7188 -0.17 1 130 6.2e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3602 129 – 161 872.6868 3486.7183 3486.7188 -0.16 1 175 2.1e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3604 129 – 161 698.3511 3486.7189 3486.7188 0.031 1 69 0.00083 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3605 129 – 161 872.6871 3486.7192 3486.7188 0.10 1 159 7.8e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3607 129 – 161 872.6872 3486.7198 3486.7188 0.28 1 57 0.012 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3608 129 – 161 872.6872 3486.7199 3486.7188 0.30 1 69 0.0007 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3609 129 – 161 698.3513 3486.7199 3486.7188 0.30 1 92 4.4e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3610 129 – 161 698.3513 3486.7199 3486.7188 0.30 1 105 1.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3611 129 – 161 872.6872 3486.7199 3486.7188 0.31 1 150 5.9e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3616 129 – 161 1163.2473 3486.7201 3486.7188 0.36 1 193 3.1e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3619 129 – 161 872.6875 3486.7210 3486.7188 0.62 1 141 4.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3623 129 – 161 872.6876 3486.7214 3486.7188 0.75 1 64 0.0022 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3624 129 – 161 872.6877 3486.7217 3486.7188 0.83 1 52 0.035 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3625 129 – 161 872.6877 3486.7217 3486.7188 0.84 1 175 1.8e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3630 129 – 161 1163.2480 3486.7222 3486.7188 0.96 1 177 1.3e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3636 129 – 161 872.6879 3486.7225 3486.7188 1.07 1 72 0.00039 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3640 129 – 161 872.6880 3486.7229 3486.7188 1.18 1 173 3.3e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3643 129 – 161 698.3520 3486.7236 3486.7188 1.36 1 80 5.7e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3644 129 – 161 872.6882 3486.7237 3486.7188 1.39 1 139 7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3648 129 – 161 872.6884 3486.7245 3486.7188 1.63 1 92 3.8e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3649 129 – 161 1163.2488 3486.7246 3486.7188 1.65 1 175 2.1e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3653 129 – 161 872.6885 3486.7249 3486.7188 1.76 1 159 7.8e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3654 129 – 161 872.6885 3486.7249 3486.7188 1.76 1 189 7.8e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3656 129 – 161 872.6885 3486.7251 3486.7188 1.80 1 146 1.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3658 129 – 161 1163.2491 3486.7255 3486.7188 1.91 1 225 1.7e-19 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3660 129 – 161 872.6887 3486.7256 3486.7188 1.95 1 131 4.7e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3673 129 – 161 872.6893 3486.7283 3486.7188 2.71 1 70 0.00052 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3674 129 – 161 1163.2501 3486.7285 3486.7188 2.77 1 186 1.6e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3676 129 – 161 1163.2505 3486.7297 3486.7188 3.11 1 164 2.1e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3677 129 – 161 1163.2510 3486.7312 3486.7188 3.55 1 207 1e-17 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3678 129 – 161 1163.2515 3486.7327 3486.7188 3.98 1 72 0.00034 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3679 129 – 161 1163.2515 3486.7327 3486.7188 3.98 1 171 4.5e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3682 129 – 161 1163.2518 3486.7336 3486.7188 4.23 1 121 4.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3684 129 – 161 1163.2520 3486.7342 3486.7188 4.41 1 76 0.00013 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3685 129 – 161 1163.2543 3486.7411 3486.7188 6.38 1 188 8.5e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3686 129 – 161 1163.2544 3486.7414 3486.7188 6.47 1 185 1.8e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3721 129 – 161 701.5493 3502.7102 3502.7137 -1.00 1 181 6e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3723 129 – 161 876.6849 3502.7103 3502.7137 -0.97 1 131 5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3725 129 – 161 876.6850 3502.7108 3502.7137 -0.84 1 113 3.6e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3728 129 – 161 584.7926 3502.7118 3502.7137 -0.56 1 71 0.00057 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3729 129 – 161 701.5496 3502.7118 3502.7137 -0.55 1 151 5.4e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3730 129 – 161 701.5497 3502.7121 3502.7137 -0.47 1 146 1.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3731 129 – 161 1168.5780 3502.7122 3502.7137 -0.44 1 140 6.6e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3732 129 – 161 876.6854 3502.7126 3502.7137 -0.32 1 113 3.5e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3734 129 – 161 701.5500 3502.7135 3502.7137 -0.060 1 136 1.8e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3737 129 – 161 876.6858 3502.7140 3502.7137 0.070 1 89 7.7e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3738 129 – 161 876.6858 3502.7141 3502.7137 0.093 1 124 2.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3741 129 – 161 876.6858 3502.7141 3502.7137 0.12 1 85 2.1e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3744 129 – 161 876.6860 3502.7147 3502.7137 0.29 1 108 1.1e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3745 129 – 161 876.6860 3502.7147 3502.7137 0.29 1 108 1.1e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3746 129 – 161 701.5503 3502.7152 3502.7137 0.43 1 108 1.1e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3756 129 – 161 876.6865 3502.7169 3502.7137 0.90 1 111 4.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3760 129 – 161 876.6866 3502.7175 3502.7137 1.08 1 121 4.7e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3763 129 – 161 876.6868 3502.7181 3502.7137 1.26 1 164 2.7e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3764 129 – 161 701.5509 3502.7182 3502.7137 1.27 1 113 3.2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3765 129 – 161 1168.5800 3502.7182 3502.7137 1.27 1 168 1e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3767 129 – 161 876.6869 3502.7184 3502.7137 1.34 1 136 1.8e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3768 129 – 161 701.5510 3502.7185 3502.7137 1.37 1 69 0.00086 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3769 129 – 161 876.6869 3502.7187 3502.7137 1.41 1 113 3.1e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3770 129 – 161 1168.5802 3502.7188 3502.7137 1.44 1 131 5.6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3771 129 – 161 701.5512 3502.7194 3502.7137 1.61 1 156 1.8e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3773 129 – 161 1168.5805 3502.7197 3502.7137 1.70 1 146 1.8e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3775 129 – 161 876.6878 3502.7222 3502.7137 2.41 1 104 2.3e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3776 129 – 161 1168.5817 3502.7233 3502.7137 2.72 1 123 3.2e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3782 129 – 161 1168.5825 3502.7257 3502.7137 3.41 1 117 1.3e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3783 129 – 161 1168.5825 3502.7257 3502.7137 3.41 1 161 5e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3785 129 – 161 1168.5827 3502.7263 3502.7137 3.58 1 79 7.9e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3786 129 – 161 876.6889 3502.7263 3502.7137 3.59 1 138 9.1e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3866 129 – 161 892.6787 3566.6857 3566.6851 0.15 1 112 6.2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Phospho (ST) 3867 129 – 161 892.6787 3566.6858 3566.6851 0.18 1 119 1.2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Phospho (ST) 3868 129 – 161 892.6789 3566.6867 3566.6851 0.43 1 97 2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Phospho (ST) 3869 129 – 161 892.6792 3566.6876 3566.6851 0.68 1 62 0.0051 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Phospho (ST) 3366 137 – 161 684.8401 2735.3314 2735.3324 -0.35 1 92 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3367 137 – 161 684.8406 2735.3333 2735.3324 0.35 1 112 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3369 137 – 161 912.7860 2735.3361 2735.3324 1.36 1 96 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3370 137 – 161 912.7862 2735.3369 2735.3324 1.67 1 119 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3383 137 – 161 688.8396 2751.3291 2751.3273 0.67 1 84 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3384 137 – 161 688.8403 2751.3322 2751.3273 1.79 1 75 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3385 137 – 161 918.1186 2751.3339 2751.3273 2.42 1 68 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3326 138 – 161 659.5791 2634.2872 2634.2847 0.95 1 92 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3327 138 – 161 659.5792 2634.2879 2634.2847 1.20 1 94 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3328 138 – 161 659.5795 2634.2889 2634.2847 1.58 1 93 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3329 138 – 161 879.1037 2634.2892 2634.2847 1.71 1 62 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3330 138 – 161 659.5796 2634.2893 2634.2847 1.74 1 66 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3331 138 – 161 879.1041 2634.2904 2634.2847 2.16 1 75 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 3332 138 – 161 879.1044 2634.2913 2634.2847 2.52 1 57 1 U T.VEDKEDVANMDNTPPVVEPPSKPK.Y 2778 142 – 159 646.9755 1937.9045 1937.9041 0.24 0 105 1 U K.EDVANMDNTPPVVEPPSK.P 2779 142 – 159 969.9615 1937.9084 1937.9041 2.23 0 80 1 U K.EDVANMDNTPPVVEPPSK.P 2780 142 – 159 969.9628 1937.9110 1937.9041 3.59 0 64 1 U K.EDVANMDNTPPVVEPPSK.P 2966 142 – 161 722.0241 2163.0506 2163.0518 -0.53 0 116 1.9e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2967 142 – 161 722.0243 2163.0512 2163.0518 -0.27 0 119 1.2e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2968 142 – 161 722.0250 2163.0532 2163.0518 0.65 0 118 1.3e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2969 142 – 161 722.0250 2163.0532 2163.0518 0.66 0 95 2.9e-06 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2970 142 – 161 722.0251 2163.0536 2163.0518 0.84 0 117 1.8e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2971 142 – 161 722.0252 2163.0537 2163.0518 0.90 0 114 3.3e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2972 142 – 161 722.0253 2163.0540 2163.0518 1.04 0 115 2.6e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2973 142 – 161 722.0254 2163.0543 2163.0518 1.15 0 59 0.011 8 U K.EDVANMDNTPPVVEPPSKPK.Y 2974 142 – 161 722.0254 2163.0543 2163.0518 1.17 0 118 1.2e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2975 142 – 161 722.0255 2163.0547 2163.0518 1.37 0 62 0.0047 4 U K.EDVANMDNTPPVVEPPSKPK.Y 2976 142 – 161 1082.5358 2163.0570 2163.0518 2.44 0 81 6.5e-05 1 U K.EDVANMDNTPPVVEPPSKPK.Y 2977 142 – 161 1082.5363 2163.0580 2163.0518 2.90 0 84 2.9e-05 7 U K.EDVANMDNTPPVVEPPSKPK.Y 2989 142 – 161 727.3567 2179.0482 2179.0467 0.71 0 105 2.6e-07 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 2991 142 – 161 727.3573 2179.0500 2179.0467 1.51 0 96 2.2e-06 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 2992 142 – 161 727.3574 2179.0503 2179.0467 1.63 0 59 0.011 5 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 2993 142 – 161 727.3574 2179.0505 2179.0467 1.76 0 72 0.00055 5 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3034 142 – 161 743.9912 2228.9517 2228.9976 -20.6 0 66 1 U K.EDVANMDNTPPVVEPPSKPK.Y + 3 [+21.9819 at E1,D2,D7] 3035 142 – 161 743.9922 2228.9547 2229.0025 -21.4 0 82 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Phospho (ST); [-14.0157 at N-term E] 3056 142 – 161 748.6815 2243.0228 2243.0545 -14.1 0 130 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M); Phospho (ST); [-15.9585 at D7]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3058 142 – 161 749.6581 2245.9525 2246.0064 -24.0 0 66 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Phospho (ST); [+2.9883 at N8] 2844 197 – 213 657.6592 1969.9557 1969.9534 1.15 0 60 1 L.SVSIEVPGEEPYHFQPR.L 2845 197 – 213 657.6594 1969.9564 1969.9534 1.55 0 73 1 L.SVSIEVPGEEPYHFQPR.L 2846 197 – 213 657.6600 1969.9583 1969.9534 2.49 0 64 1 L.SVSIEVPGEEPYHFQPR.L 2434 200 – 213 566.6152 1696.8237 1696.8209 1.62 0 90 1 S.IEVPGEEPYHFQPR.L 2435 200 – 213 566.6155 1696.8245 1696.8209 2.13 0 89 1 S.IEVPGEEPYHFQPR.L 2436 200 – 213 566.6165 1696.8277 1696.8209 4.00 0 59 1 S.IEVPGEEPYHFQPR.L 204 225 – 231 419.7379 837.4612 837.4596 1.96 0 55 0.0095 1 K.YQVLSTK.V 205 225 – 231 419.7379 837.4613 837.4596 1.98 0 53 0.016 1 K.YQVLSTK.V 207 225 – 231 419.7380 837.4614 837.4596 2.19 0 55 0.0088 1 K.YQVLSTK.V 2363 239 – 252 813.8911 1625.7677 1625.7686 -0.53 0 100 1 K.AEQVTWTTLDYSGR.P 2364 239 – 252 813.8918 1625.7691 1625.7686 0.36 0 84 1 K.AEQVTWTTLDYSGR.P 2367 239 – 252 813.8925 1625.7705 1625.7686 1.21 0 104 1 K.AEQVTWTTLDYSGR.P 2368 239 – 252 542.9309 1625.7708 1625.7686 1.35 0 70 1 K.AEQVTWTTLDYSGR.P 2369 239 – 252 542.9311 1625.7714 1625.7686 1.72 0 72 1 K.AEQVTWTTLDYSGR.P 2370 239 – 252 813.8930 1625.7715 1625.7686 1.83 0 97 1 K.AEQVTWTTLDYSGR.P 2372 239 – 252 813.8940 1625.7735 1625.7686 3.06 0 56 1 K.AEQVTWTTLDYSGR.P 2695 239 – 254 926.4655 1850.9165 1850.9163 0.14 0 72 0.0005 1 K.AEQVTWTTLDYSGRPK.A 2696 239 – 254 926.4665 1850.9185 1850.9163 1.18 0 96 1.9e-06 1 K.AEQVTWTTLDYSGRPK.A 2697 239 – 254 926.4667 1850.9188 1850.9163 1.39 0 95 2.1e-06 1 K.AEQVTWTTLDYSGRPK.A 2742 260 – 276 633.6344 1897.8812 1897.8822 -0.53 0 105 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2743 260 – 276 949.9487 1897.8829 1897.8822 0.33 0 125 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2744 260 – 276 949.9493 1897.8840 1897.8822 0.92 0 121 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2745 260 – 276 633.6353 1897.8840 1897.8822 0.95 0 95 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2746 260 – 276 633.6359 1897.8857 1897.8822 1.84 0 53 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2748 260 – 276 949.9502 1897.8859 1897.8822 1.95 0 118 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2749 260 – 276 949.9504 1897.8862 1897.8822 2.08 0 125 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2750 260 – 276 633.6360 1897.8862 1897.8822 2.11 0 97 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2751 260 – 276 633.6360 1897.8863 1897.8822 2.14 0 97 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2752 260 – 276 949.9505 1897.8863 1897.8822 2.17 0 79 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2753 260 – 276 633.6361 1897.8864 1897.8822 2.20 0 98 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2834 260 – 276 655.6018 1963.7837 1963.8152 -16.0 0 54 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+95.9435 at T3] 2837 260 – 276 491.9538 1963.7861 1963.8152 -14.8 0 56 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+95.9435 at T3] 76 270 – 276 383.1936 764.3726 764.3705 2.87 0 58 1 R.PSYPSSK.S 1645 279 – 289 454.2416 1359.7031 1359.7034 -0.22 2 56 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1646 279 – 289 340.9331 1359.7031 1359.7034 -0.21 2 60 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1650 279 – 289 454.2417 1359.7033 1359.7034 -0.11 2 63 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1653 279 – 289 454.2417 1359.7033 1359.7034 -0.061 2 55 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1654 279 – 289 454.2418 1359.7035 1359.7034 0.093 2 55 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1666 279 – 289 454.2419 1359.7040 1359.7034 0.40 2 54 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1676 279 – 289 340.9334 1359.7043 1359.7034 0.65 2 52 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1677 279 – 289 454.2421 1359.7043 1359.7034 0.67 2 71 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1707 279 – 289 454.2422 1359.7048 1359.7034 1.04 2 75 9 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1709 279 – 289 454.2422 1359.7049 1359.7034 1.06 2 53 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1724 279 – 289 454.2424 1359.7053 1359.7034 1.42 2 64 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1732 279 – 289 454.2425 1359.7056 1359.7034 1.59 2 61 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1735 279 – 289 454.2425 1359.7058 1359.7034 1.73 2 70 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1738 279 – 289 454.2426 1359.7059 1359.7034 1.84 2 59 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1056 280 – 289 411.5426 1231.6059 1231.6084 -2.10 1 68 0.0012 4 K.DWDKLEAEVK.K 1057 280 – 289 616.8104 1231.6063 1231.6084 -1.72 1 59 0.0098 1 K.DWDKLEAEVK.K 1058 280 – 289 411.5428 1231.6065 1231.6084 -1.57 1 70 0.00086 4 K.DWDKLEAEVK.K 1059 280 – 289 616.8108 1231.6070 1231.6084 -1.17 1 62 0.0053 6 K.DWDKLEAEVK.K 1060 280 – 289 616.8108 1231.6071 1231.6084 -1.08 1 54 0.033 5 K.DWDKLEAEVK.K 1062 280 – 289 616.8109 1231.6072 1231.6084 -1.02 1 64 0.0034 4 K.DWDKLEAEVK.K 1063 280 – 289 616.8109 1231.6073 1231.6084 -0.92 1 63 0.0039 5 K.DWDKLEAEVK.K 1064 280 – 289 411.5432 1231.6077 1231.6084 -0.64 1 68 0.0012 4 K.DWDKLEAEVK.K 1066 280 – 289 616.8111 1231.6077 1231.6084 -0.60 1 65 0.0026 4 K.DWDKLEAEVK.K 1067 280 – 289 616.8111 1231.6077 1231.6084 -0.58 1 62 0.0057 7 K.DWDKLEAEVK.K 1068 280 – 289 616.8112 1231.6078 1231.6084 -0.52 1 52 0.051 5 K.DWDKLEAEVK.K 1069 280 – 289 616.8112 1231.6078 1231.6084 -0.48 1 72 0.00058 5 K.DWDKLEAEVK.K 1070 280 – 289 411.5432 1231.6079 1231.6084 -0.47 1 64 0.0034 4 K.DWDKLEAEVK.K 1071 280 – 289 616.8112 1231.6079 1231.6084 -0.44 1 69 0.001 5 K.DWDKLEAEVK.K 1072 280 – 289 616.8113 1231.6080 1231.6084 -0.39 1 56 0.021 5 K.DWDKLEAEVK.K 1073 280 – 289 616.8113 1231.6080 1231.6084 -0.39 1 65 0.0026 4 K.DWDKLEAEVK.K 1074 280 – 289 616.8113 1231.6081 1231.6084 -0.31 1 69 0.001 5 K.DWDKLEAEVK.K 1075 280 – 289 616.8113 1231.6081 1231.6084 -0.29 1 52 0.052 5 K.DWDKLEAEVK.K 1076 280 – 289 411.5433 1231.6081 1231.6084 -0.28 1 67 0.0018 4 K.DWDKLEAEVK.K 1077 280 – 289 411.5433 1231.6081 1231.6084 -0.25 1 69 0.00099 1 K.DWDKLEAEVK.K 1078 280 – 289 616.8114 1231.6082 1231.6084 -0.22 1 65 0.0029 4 K.DWDKLEAEVK.K 1079 280 – 289 411.5434 1231.6083 1231.6084 -0.11 1 70 0.00089 4 K.DWDKLEAEVK.K 1080 280 – 289 616.8115 1231.6084 1231.6084 -0.062 1 58 0.013 5 K.DWDKLEAEVK.K 1081 280 – 289 616.8115 1231.6084 1231.6084 -0.062 1 52 0.053 5 K.DWDKLEAEVK.K 1082 280 – 289 616.8115 1231.6084 1231.6084 -0.062 1 55 0.028 5 K.DWDKLEAEVK.K 1083 280 – 289 411.5434 1231.6084 1231.6084 -0.058 1 62 0.0057 4 K.DWDKLEAEVK.K 1084 280 – 289 616.8115 1231.6084 1231.6084 -0.029 1 63 0.0039 5 K.DWDKLEAEVK.K 1085 280 – 289 616.8115 1231.6084 1231.6084 -0.013 1 64 0.0032 4 K.DWDKLEAEVK.K 1086 280 – 289 411.5434 1231.6085 1231.6084 0.015 1 68 0.0012 4 K.DWDKLEAEVK.K 1087 280 – 289 411.5434 1231.6085 1231.6084 0.039 1 68 0.0012 4 K.DWDKLEAEVK.K 1088 280 – 289 616.8115 1231.6085 1231.6084 0.052 1 64 0.0031 4 K.DWDKLEAEVK.K 1089 280 – 289 616.8115 1231.6085 1231.6084 0.068 1 52 0.05 5 K.DWDKLEAEVK.K 1090 280 – 289 411.5435 1231.6086 1231.6084 0.088 1 55 0.027 5 K.DWDKLEAEVK.K 1091 280 – 289 616.8116 1231.6086 1231.6084 0.10 1 56 0.022 5 K.DWDKLEAEVK.K 1092 280 – 289 616.8116 1231.6086 1231.6084 0.12 1 67 0.0017 5 K.DWDKLEAEVK.K 1093 280 – 289 411.5435 1231.6086 1231.6084 0.14 1 66 0.002 4 K.DWDKLEAEVK.K 1094 280 – 289 411.5435 1231.6086 1231.6084 0.14 1 65 0.0023 4 K.DWDKLEAEVK.K 1095 280 – 289 616.8116 1231.6086 1231.6084 0.15 1 58 0.014 5 K.DWDKLEAEVK.K 1096 280 – 289 411.5435 1231.6086 1231.6084 0.16 1 65 0.0026 4 K.DWDKLEAEVK.K 1097 280 – 289 616.8116 1231.6087 1231.6084 0.23 1 63 0.0046 5 K.DWDKLEAEVK.K 1098 280 – 289 616.8116 1231.6087 1231.6084 0.25 1 55 0.029 5 K.DWDKLEAEVK.K 1099 280 – 289 616.8117 1231.6088 1231.6084 0.26 1 52 0.048 5 K.DWDKLEAEVK.K 1100 280 – 289 616.8117 1231.6088 1231.6084 0.28 1 57 0.015 5 K.DWDKLEAEVK.K 1101 280 – 289 411.5435 1231.6088 1231.6084 0.31 1 66 0.0022 4 K.DWDKLEAEVK.K 1102 280 – 289 616.8117 1231.6088 1231.6084 0.31 1 64 0.0033 5 K.DWDKLEAEVK.K 1103 280 – 289 616.8117 1231.6089 1231.6084 0.38 1 61 0.0069 3 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1104 280 – 289 616.8117 1231.6089 1231.6084 0.39 1 62 0.0054 4 K.DWDKLEAEVK.K 1105 280 – 289 411.5436 1231.6089 1231.6084 0.40 1 65 0.0025 6 K.DWDKLEAEVK.K 1106 280 – 289 616.8118 1231.6090 1231.6084 0.43 1 61 0.0073 5 K.DWDKLEAEVK.K 1107 280 – 289 411.5436 1231.6090 1231.6084 0.43 1 66 0.0022 4 K.DWDKLEAEVK.K 1108 280 – 289 616.8118 1231.6090 1231.6084 0.44 1 65 0.0026 4 K.DWDKLEAEVK.K 1109 280 – 289 411.5436 1231.6090 1231.6084 0.45 1 66 0.002 4 K.DWDKLEAEVK.K 1110 280 – 289 411.5436 1231.6090 1231.6084 0.48 1 66 0.002 4 K.DWDKLEAEVK.K 1112 280 – 289 411.5436 1231.6091 1231.6084 0.50 1 74 0.00033 4 K.DWDKLEAEVK.K 1113 280 – 289 411.5436 1231.6091 1231.6084 0.50 1 65 0.0026 4 K.DWDKLEAEVK.K 1114 280 – 289 616.8118 1231.6091 1231.6084 0.51 1 53 0.037 5 K.DWDKLEAEVK.K 1115 280 – 289 411.5436 1231.6091 1231.6084 0.53 1 67 0.0018 4 K.DWDKLEAEVK.K 1116 280 – 289 411.5436 1231.6091 1231.6084 0.53 1 65 0.0028 4 K.DWDKLEAEVK.K 1117 280 – 289 411.5437 1231.6091 1231.6084 0.55 1 61 0.0072 1 K.DWDKLEAEVK.K 1118 280 – 289 616.8118 1231.6091 1231.6084 0.56 1 62 0.0051 4 K.DWDKLEAEVK.K 1119 280 – 289 616.8119 1231.6091 1231.6084 0.57 1 56 0.021 1 K.DWDKLEAEVK.K 1120 280 – 289 616.8119 1231.6092 1231.6084 0.59 1 56 0.022 5 K.DWDKLEAEVK.K 1121 280 – 289 411.5437 1231.6092 1231.6084 0.60 1 75 0.00027 4 K.DWDKLEAEVK.K 1123 280 – 289 411.5437 1231.6092 1231.6084 0.60 1 67 0.0018 4 K.DWDKLEAEVK.K 1124 280 – 289 616.8119 1231.6092 1231.6084 0.60 1 63 0.0038 5 K.DWDKLEAEVK.K 1125 280 – 289 411.5437 1231.6092 1231.6084 0.62 1 68 0.0014 4 K.DWDKLEAEVK.K 1126 280 – 289 616.8119 1231.6092 1231.6084 0.64 1 56 0.022 5 K.DWDKLEAEVK.K 1127 280 – 289 616.8119 1231.6092 1231.6084 0.65 1 56 0.022 5 K.DWDKLEAEVK.K 1128 280 – 289 616.8119 1231.6093 1231.6084 0.67 1 63 0.0039 4 K.DWDKLEAEVK.K 1129 280 – 289 616.8119 1231.6093 1231.6084 0.67 1 54 0.031 5 K.DWDKLEAEVK.K 1130 280 – 289 411.5437 1231.6093 1231.6084 0.67 1 54 0.03 4 K.DWDKLEAEVK.K 1131 280 – 289 616.8119 1231.6093 1231.6084 0.70 1 60 0.0076 5 K.DWDKLEAEVK.K 1132 280 – 289 411.5437 1231.6093 1231.6084 0.72 1 65 0.0024 4 K.DWDKLEAEVK.K 1133 280 – 289 411.5437 1231.6093 1231.6084 0.72 1 59 0.011 1 K.DWDKLEAEVK.K 1134 280 – 289 616.8120 1231.6093 1231.6084 0.73 1 61 0.0069 5 K.DWDKLEAEVK.K 1135 280 – 289 411.5437 1231.6094 1231.6084 0.75 1 71 0.00061 4 K.DWDKLEAEVK.K 1136 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 59 0.011 4 K.DWDKLEAEVK.K 1137 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 59 0.011 5 K.DWDKLEAEVK.K 1138 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 65 0.0028 5 K.DWDKLEAEVK.K 1139 280 – 289 616.8120 1231.6094 1231.6084 0.77 1 56 0.02 7 K.DWDKLEAEVK.K 1140 280 – 289 616.8120 1231.6094 1231.6084 0.77 1 67 0.0018 4 K.DWDKLEAEVK.K 1141 280 – 289 411.5437 1231.6094 1231.6084 0.77 1 64 0.0032 4 K.DWDKLEAEVK.K 1142 280 – 289 411.5437 1231.6094 1231.6084 0.77 1 65 0.0023 4 K.DWDKLEAEVK.K 1143 280 – 289 411.5437 1231.6094 1231.6084 0.79 1 66 0.0021 1 K.DWDKLEAEVK.K 1144 280 – 289 616.8120 1231.6094 1231.6084 0.80 1 62 0.0053 6 K.DWDKLEAEVK.K 1145 280 – 289 616.8120 1231.6094 1231.6084 0.82 1 52 0.048 5 K.DWDKLEAEVK.K 1146 280 – 289 616.8120 1231.6095 1231.6084 0.83 1 58 0.012 7 K.DWDKLEAEVK.K 1147 280 – 289 616.8120 1231.6095 1231.6084 0.85 1 62 0.0055 4 K.DWDKLEAEVK.K 1148 280 – 289 411.5438 1231.6095 1231.6084 0.87 1 69 0.00093 4 K.DWDKLEAEVK.K 1149 280 – 289 616.8120 1231.6095 1231.6084 0.88 1 58 0.012 4 K.DWDKLEAEVK.K 1150 280 – 289 411.5438 1231.6095 1231.6084 0.89 1 71 0.00068 4 K.DWDKLEAEVK.K 1151 280 – 289 411.5438 1231.6095 1231.6084 0.89 1 69 0.0011 4 K.DWDKLEAEVK.K 1152 280 – 289 411.5438 1231.6096 1231.6084 0.92 1 71 0.00064 4 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1153 280 – 289 411.5438 1231.6096 1231.6084 0.92 1 60 0.0082 5 K.DWDKLEAEVK.K 1154 280 – 289 411.5438 1231.6096 1231.6084 0.94 1 72 0.00052 1 K.DWDKLEAEVK.K 1155 280 – 289 411.5438 1231.6096 1231.6084 0.94 1 72 0.00052 1 K.DWDKLEAEVK.K 1156 280 – 289 411.5438 1231.6096 1231.6084 0.94 1 69 0.0011 4 K.DWDKLEAEVK.K 1158 280 – 289 616.8121 1231.6096 1231.6084 0.95 1 60 0.0078 5 K.DWDKLEAEVK.K 1159 280 – 289 616.8121 1231.6096 1231.6084 0.96 1 66 0.002 4 K.DWDKLEAEVK.K 1160 280 – 289 616.8121 1231.6096 1231.6084 0.98 1 63 0.0037 5 K.DWDKLEAEVK.K 1161 280 – 289 616.8121 1231.6096 1231.6084 0.98 1 65 0.0028 5 K.DWDKLEAEVK.K 1162 280 – 289 616.8121 1231.6097 1231.6084 0.99 1 62 0.0056 5 K.DWDKLEAEVK.K 1163 280 – 289 411.5438 1231.6097 1231.6084 1.01 1 66 0.0021 4 K.DWDKLEAEVK.K 1164 280 – 289 616.8121 1231.6097 1231.6084 1.03 1 72 0.00057 5 K.DWDKLEAEVK.K 1165 280 – 289 616.8121 1231.6097 1231.6084 1.03 1 64 0.0031 4 K.DWDKLEAEVK.K 1166 280 – 289 411.5439 1231.6097 1231.6084 1.04 1 62 0.0053 4 K.DWDKLEAEVK.K 1167 280 – 289 411.5439 1231.6097 1231.6084 1.04 1 65 0.0026 4 K.DWDKLEAEVK.K 1168 280 – 289 616.8121 1231.6097 1231.6084 1.04 1 61 0.007 5 K.DWDKLEAEVK.K 1169 280 – 289 616.8121 1231.6097 1231.6084 1.06 1 67 0.0016 5 K.DWDKLEAEVK.K 1170 280 – 289 411.5439 1231.6098 1231.6084 1.09 1 66 0.0019 4 K.DWDKLEAEVK.K 1171 280 – 289 616.8122 1231.6098 1231.6084 1.09 1 60 0.0086 4 K.DWDKLEAEVK.K 1172 280 – 289 616.8122 1231.6098 1231.6084 1.11 1 58 0.012 5 K.DWDKLEAEVK.K 1173 280 – 289 411.5439 1231.6098 1231.6084 1.11 1 65 0.0027 4 K.DWDKLEAEVK.K 1174 280 – 289 616.8122 1231.6098 1231.6084 1.12 1 61 0.0072 5 K.DWDKLEAEVK.K 1175 280 – 289 411.5439 1231.6098 1231.6084 1.14 1 67 0.0015 4 K.DWDKLEAEVK.K 1176 280 – 289 616.8122 1231.6098 1231.6084 1.14 1 57 0.017 5 K.DWDKLEAEVK.K 1177 280 – 289 411.5439 1231.6099 1231.6084 1.16 1 72 0.00046 4 K.DWDKLEAEVK.K 1178 280 – 289 411.5439 1231.6099 1231.6084 1.16 1 69 0.0011 4 K.DWDKLEAEVK.K 1179 280 – 289 616.8122 1231.6099 1231.6084 1.17 1 54 0.031 7 K.DWDKLEAEVK.K 1180 280 – 289 411.5439 1231.6099 1231.6084 1.18 1 65 0.0024 4 K.DWDKLEAEVK.K 1181 280 – 289 616.8122 1231.6099 1231.6084 1.20 1 59 0.0092 5 K.DWDKLEAEVK.K 1183 280 – 289 411.5439 1231.6099 1231.6084 1.21 1 66 0.0019 4 K.DWDKLEAEVK.K 1184 280 – 289 411.5439 1231.6099 1231.6084 1.21 1 66 0.002 4 K.DWDKLEAEVK.K 1185 280 – 289 411.5439 1231.6099 1231.6084 1.21 1 65 0.0026 4 K.DWDKLEAEVK.K 1186 280 – 289 411.5439 1231.6099 1231.6084 1.21 1 68 0.0014 4 K.DWDKLEAEVK.K 1187 280 – 289 616.8122 1231.6099 1231.6084 1.22 1 62 0.0053 4 K.DWDKLEAEVK.K 1188 280 – 289 616.8122 1231.6099 1231.6084 1.22 1 53 0.039 5 K.DWDKLEAEVK.K 1189 280 – 289 616.8123 1231.6100 1231.6084 1.24 1 54 0.034 5 K.DWDKLEAEVK.K 1190 280 – 289 616.8123 1231.6100 1231.6084 1.25 1 58 0.012 5 K.DWDKLEAEVK.K 1191 280 – 289 616.8123 1231.6100 1231.6084 1.25 1 69 0.00097 5 K.DWDKLEAEVK.K 1192 280 – 289 616.8123 1231.6100 1231.6084 1.25 1 64 0.003 4 K.DWDKLEAEVK.K 1194 280 – 289 616.8123 1231.6100 1231.6084 1.27 1 55 0.028 7 K.DWDKLEAEVK.K 1195 280 – 289 411.5439 1231.6100 1231.6084 1.28 1 75 0.00023 1 K.DWDKLEAEVK.K 1196 280 – 289 411.5439 1231.6100 1231.6084 1.28 1 82 5.7e-05 4 K.DWDKLEAEVK.K 1197 280 – 289 616.8123 1231.6100 1231.6084 1.29 1 57 0.015 4 K.DWDKLEAEVK.K 1198 280 – 289 616.8123 1231.6100 1231.6084 1.29 1 52 0.051 4 K.DWDKLEAEVK.K 1199 280 – 289 616.8123 1231.6100 1231.6084 1.29 1 72 0.00054 5 K.DWDKLEAEVK.K 1200 280 – 289 616.8123 1231.6100 1231.6084 1.29 1 60 0.0088 7 K.DWDKLEAEVK.K 1201 280 – 289 411.5440 1231.6101 1231.6084 1.31 1 72 0.00054 1 K.DWDKLEAEVK.K 1202 280 – 289 411.5440 1231.6101 1231.6084 1.31 1 71 0.00063 4 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1254 280 – 289 411.5441 1231.6105 1231.6084 1.70 1 69 0.0011 1 K.DWDKLEAEVK.K 1255 280 – 289 616.8125 1231.6105 1231.6084 1.71 1 70 0.00077 4 K.DWDKLEAEVK.K 1256 280 – 289 411.5441 1231.6106 1231.6084 1.72 1 67 0.0018 4 K.DWDKLEAEVK.K 1257 280 – 289 616.8126 1231.6106 1231.6084 1.72 1 56 0.02 4 K.DWDKLEAEVK.K 1258 280 – 289 616.8126 1231.6106 1231.6084 1.74 1 54 0.033 5 K.DWDKLEAEVK.K 1259 280 – 289 411.5441 1231.6106 1231.6084 1.74 1 74 0.00032 4 K.DWDKLEAEVK.K 1260 280 – 289 411.5441 1231.6106 1231.6084 1.74 1 67 0.0015 4 K.DWDKLEAEVK.K 1261 280 – 289 616.8126 1231.6106 1231.6084 1.76 1 66 0.0021 4 K.DWDKLEAEVK.K 1262 280 – 289 616.8126 1231.6106 1231.6084 1.77 1 57 0.015 1 K.DWDKLEAEVK.K 1264 280 – 289 616.8126 1231.6107 1231.6084 1.81 1 71 0.00056 5 K.DWDKLEAEVK.K 1266 280 – 289 411.5442 1231.6107 1231.6084 1.84 1 63 0.0036 4 K.DWDKLEAEVK.K 1267 280 – 289 411.5442 1231.6107 1231.6084 1.84 1 66 0.002 4 K.DWDKLEAEVK.K 1269 280 – 289 411.5442 1231.6107 1231.6084 1.87 1 52 0.054 1 K.DWDKLEAEVK.K 1270 280 – 289 411.5442 1231.6108 1231.6084 1.89 1 65 0.0025 4 K.DWDKLEAEVK.K 1271 280 – 289 616.8127 1231.6108 1231.6084 1.90 1 58 0.012 7 K.DWDKLEAEVK.K 1272 280 – 289 411.5442 1231.6108 1231.6084 1.91 1 70 0.0008 4 K.DWDKLEAEVK.K 1273 280 – 289 411.5442 1231.6108 1231.6084 1.94 1 72 0.0005 1 K.DWDKLEAEVK.K 1274 280 – 289 616.8127 1231.6109 1231.6084 1.98 1 56 0.018 5 K.DWDKLEAEVK.K 1275 280 – 289 411.5442 1231.6109 1231.6084 2.01 1 64 0.0029 4 K.DWDKLEAEVK.K 1276 280 – 289 411.5443 1231.6110 1231.6084 2.04 1 66 0.0021 1 K.DWDKLEAEVK.K 1277 280 – 289 411.5443 1231.6110 1231.6084 2.06 1 68 0.0012 4 K.DWDKLEAEVK.K 1278 280 – 289 616.8128 1231.6111 1231.6084 2.15 1 71 0.00065 5 K.DWDKLEAEVK.K 1279 280 – 289 616.8128 1231.6111 1231.6084 2.16 1 54 0.032 4 K.DWDKLEAEVK.K 1280 280 – 289 411.5443 1231.6112 1231.6084 2.23 1 68 0.0011 4 K.DWDKLEAEVK.K 1281 280 – 289 616.8129 1231.6113 1231.6084 2.31 1 56 0.023 5 K.DWDKLEAEVK.K 1282 280 – 289 411.5444 1231.6114 1231.6084 2.38 1 64 0.0034 4 K.DWDKLEAEVK.K 1283 280 – 289 411.5445 1231.6116 1231.6084 2.55 1 64 0.0031 4 K.DWDKLEAEVK.K 1290 280 – 289 412.8752 1235.6038 1235.6034 0.38 1 55 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1291 280 – 289 618.8095 1235.6044 1235.6034 0.85 1 51 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1312 280 – 289 624.8085 1247.6024 1247.6034 -0.75 1 55 2 K.DWDKLEAEVK.K + [+15.9949 at N-term D] 1314 280 – 289 624.8085 1247.6025 1247.6034 -0.69 1 53 1 K.DWDKLEAEVK.K + [+15.9949 at N-term D] 1316 280 – 289 624.8092 1247.6039 1247.6034 0.44 1 67 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1317 280 – 289 624.8094 1247.6042 1247.6034 0.65 1 63 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1318 280 – 289 624.8094 1247.6042 1247.6034 0.68 1 64 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1320 280 – 289 624.8096 1247.6046 1247.6034 1.01 1 81 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1321 280 – 289 416.8756 1247.6051 1247.6034 1.36 1 87 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1322 280 – 289 624.8099 1247.6053 1247.6034 1.56 1 62 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1323 280 – 289 624.8100 1247.6055 1247.6034 1.70 1 86 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1325 280 – 289 416.8758 1247.6056 1247.6034 1.82 1 78 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1327 280 – 289 416.8759 1247.6058 1247.6034 1.96 1 64 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1329 280 – 289 416.8760 1247.6061 1247.6034 2.18 1 68 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1342 280 – 289 627.7985 1253.5824 1253.5904 -6.39 1 63 1 K.DWDKLEAEVK.K + [+21.9819 at E6] 1343 280 – 289 627.7989 1253.5833 1253.5904 -5.65 1 55 1 K.DWDKLEAEVK.K + [+21.9819 at E6] 1344 280 – 289 627.7997 1253.5849 1253.5904 -4.41 1 61 1 K.DWDKLEAEVK.K + [+21.9819 at E6] 1353 280 – 289 629.8202 1257.6259 1257.6241 1.47 1 55 1 K.DWDKLEAEVK.K + [+26.0156 at N-term] 1357 280 – 289 630.8086 1259.6027 1259.6034 -0.55 1 53 1 K.DWDKLEAEVK.K + [+27.9949 at N-term] 1358 280 – 289 630.8091 1259.6037 1259.6034 0.27 1 52 1 K.DWDKLEAEVK.K + [+27.9949 at K4]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1359 280 – 289 630.8102 1259.6058 1259.6034 1.93 1 53 1 K.DWDKLEAEVK.K + [+27.9949 at K4] 1362 280 – 289 632.8064 1263.5983 1263.5983 0.048 1 54 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1365 280 – 289 632.8067 1263.5988 1263.5983 0.44 1 53 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1366 280 – 289 422.2069 1263.5989 1263.5983 0.50 1 54 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1367 280 – 289 632.8068 1263.5991 1263.5983 0.65 1 52 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1368 280 – 289 632.8071 1263.5995 1263.5983 1.01 1 52 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1369 280 – 289 422.2072 1263.5997 1263.5983 1.17 1 58 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1370 280 – 289 422.2073 1263.6000 1263.5983 1.38 1 54 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1371 280 – 289 422.2074 1263.6003 1263.5983 1.62 1 56 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 2441 291 – 305 568.2880 1701.8421 1701.8421 0.033 2 67 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2443 291 – 305 568.2884 1701.8433 1701.8421 0.72 2 78 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2446 291 – 305 568.2885 1701.8438 1701.8421 1.02 2 124 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2452 291 – 305 568.2886 1701.8440 1701.8421 1.16 2 118 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2453 291 – 305 568.2886 1701.8441 1701.8421 1.21 2 107 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2457 291 – 305 568.2891 1701.8454 1701.8421 1.99 2 118 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2458 291 – 305 851.9301 1701.8456 1701.8421 2.06 2 95 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E2] 2464 291 – 305 426.4688 1701.8463 1701.8421 2.49 2 58 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2469 291 – 305 851.9311 1701.8476 1701.8421 3.26 2 76 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E2] 2599 291 – 305 604.6394 1810.8963 1810.8948 0.83 2 63 1 K.EEKEEKLEGDAALNK.F + [+109.0528 at K6] 2600 291 – 305 604.6398 1810.8975 1810.9510 -29.6 2 78 3 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); 3 [+17.0345 at E1,E2,E4] 2601 291 – 305 604.6399 1810.8977 1810.9510 -29.4 2 70 2 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); 3 [+17.0345 at E1,E2,E4] 2607 291 – 305 454.2335 1812.9050 1812.8741 17.0 2 62 1 K.EEKEEKLEGDAALNK.F + [+111.0320 at K6] 2608 291 – 305 454.2362 1812.9159 1812.8741 23.1 2 59 1 K.EEKEEKLEGDAALNK.F + [+111.0320 at K6] 2620 291 – 305 610.9855 1829.9347 1829.9370 -1.24 2 114 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2630 291 – 305 610.9864 1829.9375 1829.9370 0.29 2 133 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2633 291 – 305 610.9866 1829.9380 1829.9370 0.55 2 99 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2642 291 – 305 458.4920 1829.9388 1829.9370 0.97 2 95 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2645 291 – 305 610.9869 1829.9389 1829.9370 1.06 2 130 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2649 291 – 305 458.4921 1829.9392 1829.9370 1.19 2 74 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2652 291 – 305 610.9871 1829.9394 1829.9370 1.30 2 142 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2653 291 – 305 458.4921 1829.9394 1829.9370 1.32 2 59 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2656 291 – 305 610.9872 1829.9398 1829.9370 1.53 2 65 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2658 291 – 305 610.9872 1829.9398 1829.9370 1.55 2 80 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2660 291 – 305 458.4923 1829.9400 1829.9370 1.63 2 85 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2665 291 – 305 458.4923 1829.9403 1829.9370 1.80 2 63 1 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2669 291 – 305 610.9874 1829.9403 1829.9370 1.83 2 111 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2670 291 – 305 610.9874 1829.9404 1829.9370 1.84 2 75 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2671 291 – 305 458.4924 1829.9404 1829.9370 1.87 2 81 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2672 291 – 305 458.4924 1829.9404 1829.9370 1.87 2 55 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2674 291 – 305 610.9874 1829.9405 1829.9370 1.89 2 71 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2676 291 – 305 458.4925 1829.9407 1829.9370 2.04 2 87 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2677 291 – 305 458.4925 1829.9408 1829.9370 2.09 2 59 1 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2678 291 – 305 610.9876 1829.9409 1829.9370 2.12 2 55 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2679 291 – 305 458.4925 1829.9409 1829.9370 2.13 2 93 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2731 291 – 305 632.9530 1895.8372 1895.8764 -20.7 2 52 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2732 291 – 305 632.9535 1895.8387 1895.8764 -19.9 2 57 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 1445 294 – 305 433.5580 1297.6521 1297.6514 0.54 1 52 2 K.EEKLEGDAALNK.F + [-18.0106 at N-term]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1447 294 – 305 433.5582 1297.6527 1297.6514 1.02 1 62 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1448 294 – 305 433.5590 1297.6551 1297.6514 2.90 1 65 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1449 294 – 305 433.5591 1297.6555 1297.6514 3.20 1 58 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1486 294 – 305 439.5605 1315.6597 1315.6619 -1.68 1 90 6.2e-06 1 K.EEKLEGDAALNK.F 1493 294 – 305 439.5614 1315.6623 1315.6619 0.33 1 90 6.5e-06 1 K.EEKLEGDAALNK.F 1495 294 – 305 658.8387 1315.6629 1315.6619 0.73 1 68 0.00094 1 K.EEKLEGDAALNK.F 1496 294 – 305 658.8388 1315.6630 1315.6619 0.85 1 64 0.0028 7 K.EEKLEGDAALNK.F 1497 294 – 305 439.5616 1315.6631 1315.6619 0.92 1 54 0.028 1 K.EEKLEGDAALNK.F 1498 294 – 305 439.5618 1315.6635 1315.6619 1.17 1 54 0.025 7 K.EEKLEGDAALNK.F 1500 294 – 305 658.8391 1315.6636 1315.6619 1.31 1 83 3.1e-05 1 K.EEKLEGDAALNK.F 1502 294 – 305 658.8391 1315.6637 1315.6619 1.35 1 79 8.4e-05 1 K.EEKLEGDAALNK.F 1503 294 – 305 658.8392 1315.6637 1315.6619 1.40 1 64 0.003 1 K.EEKLEGDAALNK.F 1504 294 – 305 658.8392 1315.6637 1315.6619 1.40 1 59 0.0084 7 K.EEKLEGDAALNK.F 1505 294 – 305 658.8393 1315.6640 1315.6619 1.62 1 63 0.0037 1 K.EEKLEGDAALNK.F 1506 294 – 305 658.8393 1315.6640 1315.6619 1.62 1 71 0.00048 1 K.EEKLEGDAALNK.F 1509 294 – 305 658.8397 1315.6647 1315.6619 2.16 1 83 3.5e-05 1 K.EEKLEGDAALNK.F 1510 294 – 305 439.5622 1315.6648 1315.6619 2.17 1 94 2.7e-06 1 K.EEKLEGDAALNK.F 1512 294 – 305 658.8398 1315.6650 1315.6619 2.32 1 74 0.00027 1 K.EEKLEGDAALNK.F 1513 294 – 305 658.8398 1315.6651 1315.6619 2.46 1 79 8e-05 1 K.EEKLEGDAALNK.F 1515 294 – 305 439.5624 1315.6653 1315.6619 2.59 1 94 2.6e-06 1 K.EEKLEGDAALNK.F 1516 294 – 305 658.8400 1315.6653 1315.6619 2.61 1 56 0.018 1 K.EEKLEGDAALNK.F 1571 294 – 305 669.8283 1337.6421 1337.6439 -1.29 1 57 1 K.EEKLEGDAALNK.F + [+21.9819 at D7] 407 297 – 305 465.7485 929.4824 929.4818 0.67 0 74 0.00029 1 K.LEGDAALNK.F 408 297 – 305 465.7492 929.4839 929.4818 2.25 0 72 0.00048 1 K.LEGDAALNK.F 1752 297 – 308 681.3468 1360.6791 1360.7099 -22.7 1 55 1 K.LEGDAALNKFFR.D + [-19.0098 at F10] 1756 297 – 308 681.3473 1360.6800 1360.7099 -22.0 1 59 1 K.LEGDAALNKFFR.D + [-19.0098 at F10] 1776 309 – 319 685.7977 1369.5809 1369.5820 -0.80 1 93 4.1e-06 1 R.DIYKDADEDMR.R 1778 309 – 319 685.7981 1369.5817 1369.5820 -0.18 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1779 309 – 319 685.7982 1369.5819 1369.5820 -0.074 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1780 309 – 319 457.5348 1369.5826 1369.5820 0.46 1 81 7.3e-05 1 R.DIYKDADEDMR.R 1781 309 – 319 685.7986 1369.5827 1369.5820 0.57 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1782 309 – 319 685.7987 1369.5828 1369.5820 0.63 1 103 4.3e-07 1 R.DIYKDADEDMR.R 1784 309 – 319 685.7988 1369.5830 1369.5820 0.73 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1785 309 – 319 457.5350 1369.5831 1369.5820 0.79 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1786 309 – 319 457.5351 1369.5835 1369.5820 1.14 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1787 309 – 319 457.5351 1369.5836 1369.5820 1.18 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1788 309 – 319 457.5352 1369.5839 1369.5820 1.38 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1789 309 – 319 457.5353 1369.5840 1369.5820 1.47 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1790 309 – 319 685.7993 1369.5840 1369.5820 1.49 1 103 4.3e-07 1 R.DIYKDADEDMR.R 1791 309 – 319 685.7993 1369.5840 1369.5820 1.49 1 93 4.2e-06 1 R.DIYKDADEDMR.R 1792 309 – 319 457.5355 1369.5846 1369.5820 1.91 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1793 309 – 319 457.5355 1369.5847 1369.5820 1.97 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1794 309 – 319 457.5356 1369.5851 1369.5820 2.26 1 60 0.0079 1 R.DIYKDADEDMR.R 1795 309 – 319 685.7998 1369.5851 1369.5820 2.32 1 80 8.4e-05 1 R.DIYKDADEDMR.R 1796 309 – 319 457.5358 1369.5855 1369.5820 2.59 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1797 309 – 319 685.8005 1369.5865 1369.5820 3.31 1 78 0.00014 1 R.DIYKDADEDMR.R 1839 309 – 319 462.8645 1385.5716 1385.5769 -3.83 1 83 3.4e-05 1 R.DIYKDADEDMR.R + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1840 309 – 319 693.7950 1385.5755 1385.5769 -1.01 1 76 0.00018 1 R.DIYKDADEDMR.R + Oxidation (M) 1841 309 – 319 693.7961 1385.5776 1385.5769 0.55 1 63 0.0033 1 R.DIYKDADEDMR.R + Oxidation (M) 1842 309 – 319 462.8665 1385.5777 1385.5769 0.59 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1843 309 – 319 462.8666 1385.5781 1385.5769 0.89 1 82 4.3e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1844 309 – 319 693.7965 1385.5784 1385.5769 1.11 1 64 0.003 1 R.DIYKDADEDMR.R + Oxidation (M) 1845 309 – 319 462.8668 1385.5787 1385.5769 1.30 1 78 0.00011 1 R.DIYKDADEDMR.R + Oxidation (M) 1846 309 – 319 693.7969 1385.5792 1385.5769 1.70 1 74 0.00032 1 R.DIYKDADEDMR.R + Oxidation (M) 1847 309 – 319 693.7970 1385.5794 1385.5769 1.80 1 73 0.0004 1 R.DIYKDADEDMR.R + Oxidation (M) 1849 309 – 319 462.8674 1385.5804 1385.5769 2.51 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1879 309 – 319 696.7880 1391.5614 1391.5639 -1.78 1 61 1 R.DIYKDADEDMR.R + [+21.9819 at D5] 1905 309 – 319 470.1839 1407.5299 1407.5379 -5.65 1 57 2 R.DIYKDADEDMR.R + [+37.9559 at D5] 1922 309 – 319 706.3113 1410.6080 1410.6085 -0.36 1 80 1 R.DIYKDADEDMR.R + [+41.0265 at K4] 1974 309 – 319 718.7479 1435.4812 1435.4963 -10.5 1 66 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1975 309 – 319 718.7480 1435.4814 1435.4963 -10.4 1 70 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1976 309 – 319 718.7480 1435.4814 1435.4963 -10.4 1 57 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1977 309 – 319 718.7483 1435.4821 1435.4963 -9.88 1 74 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1978 309 – 319 718.7484 1435.4822 1435.4963 -9.80 1 74 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1985 309 – 319 718.7494 1435.4843 1435.4963 -8.34 1 62 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1987 309 – 319 479.5023 1435.4851 1435.4963 -7.78 1 58 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1988 309 – 319 718.7501 1435.4857 1435.4963 -7.35 1 62 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 1777 309 – 320 685.7981 1369.5817 1369.5820 -0.19 2 74 1 R.DIYKDADEDMRR.A + [-156.1011 at C-term] 2106 309 – 320 504.5613 1510.6622 1510.6722 -6.64 2 61 1 R.DIYKDADEDMRR.A + [-15.0109 at N-term] 2144 309 – 320 509.5674 1525.6804 1525.7195 -25.6 2 84 1 R.DIYKDADEDMRR.A + Oxidation (M); [-15.9585 at E8] 2332 309 – 320 531.5355 1591.5847 1591.5974 -7.99 2 60 1 R.DIYKDADEDMRR.A + Phospho (Y); [-14.0520 at K4] 838 311 – 319 571.7425 1141.4705 1141.4710 -0.38 1 80 1 I.YKDADEDMR.R 839 311 – 319 571.7427 1141.4709 1141.4710 -0.066 1 65 1 I.YKDADEDMR.R 841 311 – 319 571.7431 1141.4716 1141.4710 0.60 1 64 1 I.YKDADEDMR.R 242 313 – 319 426.1642 850.3138 850.3127 1.30 0 62 0.0023 1 K.DADEDMR.R 243 313 – 319 426.1644 850.3142 850.3127 1.84 0 62 0.0023 1 K.DADEDMR.R 244 313 – 319 426.1648 850.3150 850.3127 2.69 0 59 0.0045 1 K.DADEDMR.R 245 313 – 319 426.1648 850.3150 850.3127 2.76 0 66 0.00095 1 K.DADEDMR.R 246 313 – 319 426.1649 850.3152 850.3127 2.92 0 66 0.00095 1 K.DADEDMR.R 247 313 – 319 426.1651 850.3155 850.3127 3.37 0 62 0.0023 1 K.DADEDMR.R 248 313 – 319 426.1652 850.3158 850.3127 3.68 0 66 0.00099 1 K.DADEDMR.R 556 313 – 320 504.2154 1006.4163 1006.4138 2.54 1 53 0.023 1 K.DADEDMRR.A 1555 328 – 339 668.3315 1334.6484 1334.6466 1.36 0 76 0.00021 1 U R.ESNGTVLSTNWK.D 1556 328 – 339 668.3316 1334.6487 1334.6466 1.52 0 79 0.00011 1 U R.ESNGTVLSTNWK.D 1557 328 – 339 668.3316 1334.6487 1334.6466 1.52 0 73 0.00039 1 U R.ESNGTVLSTNWK.D 1558 328 – 339 668.3316 1334.6487 1334.6466 1.57 0 71 0.00056 1 U R.ESNGTVLSTNWK.D 1562 328 – 339 668.8207 1335.6268 1335.6500 -17.3 0 72 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1563 328 – 339 668.8213 1335.6281 1335.6500 -16.4 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1564 328 – 339 668.8222 1335.6299 1335.6500 -15.0 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1565 328 – 339 668.8241 1335.6336 1335.6500 -12.3 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1566 328 – 339 669.3153 1336.6161 1336.6147 1.06 0 68 1 U R.ESNGTVLSTNWK.D + 2 [+0.9840 at N3,N10] 1568 328 – 339 669.3179 1336.6212 1336.6534 -24.1 0 65 1 U R.ESNGTVLSTNWK.D + 2 [+1.0034 at S2,N3] 2699 340 – 357 620.9734 1859.8983 1859.8953 1.65 1 64 1 U K.DVGSKTVEASPPDGMELK.K + [+0.9970 at S4] 2754 340 – 357 634.3161 1899.9266 1899.9248 0.95 1 88 1 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2755 340 – 357 634.3164 1899.9275 1899.9248 1.42 1 64 1 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2756 340 – 357 634.3166 1899.9280 1899.9248 1.69 1 93 2 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2757 340 – 357 634.3177 1899.9313 1899.9248 3.44 1 59 3 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 1803 345 – 357 687.3347 1372.6548 1372.6544 0.27 0 108 1.4e-07 1 U K.TVEASPPDGMELK.K 1804 345 – 357 687.3348 1372.6550 1372.6544 0.40 0 103 3.8e-07 1 U K.TVEASPPDGMELK.K 1805 345 – 357 687.3348 1372.6551 1372.6544 0.53 0 110 8.9e-08 1 U K.TVEASPPDGMELK.K 1806 345 – 357 687.3349 1372.6553 1372.6544 0.63 0 110 8.2e-08 1 U K.TVEASPPDGMELK.K 1807 345 – 357 687.3350 1372.6555 1372.6544 0.76 0 104 3.4e-07 1 U K.TVEASPPDGMELK.K 1808 345 – 357 687.3350 1372.6555 1372.6544 0.79 0 104 3.6e-07 1 U K.TVEASPPDGMELK.K 1809 345 – 357 687.3354 1372.6562 1372.6544 1.33 0 107 1.5e-07 1 U K.TVEASPPDGMELK.K 1810 345 – 357 687.3355 1372.6564 1372.6544 1.45 0 110 8.7e-08 1 U K.TVEASPPDGMELK.K 1811 345 – 357 687.3355 1372.6565 1372.6544 1.52 0 98 1.3e-06 1 U K.TVEASPPDGMELK.K 1812 345 – 357 687.3356 1372.6567 1372.6544 1.64 0 110 8.3e-08 1 U K.TVEASPPDGMELK.K 1813 345 – 357 687.3359 1372.6572 1372.6544 2.05 0 104 3.4e-07 1 U K.TVEASPPDGMELK.K 1814 345 – 357 687.3361 1372.6577 1372.6544 2.38 0 63 0.0038 1 U K.TVEASPPDGMELK.K 1815 345 – 357 687.3365 1372.6584 1372.6544 2.91 0 91 5.8e-06 1 U K.TVEASPPDGMELK.K 1816 345 – 357 687.3366 1372.6587 1372.6544 3.14 0 72 0.00049 1 U K.TVEASPPDGMELK.K 1854 345 – 357 695.3324 1388.6503 1388.6493 0.71 0 67 0.0016 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1855 345 – 357 695.3325 1388.6504 1388.6493 0.80 0 59 0.0097 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1856 345 – 357 695.3325 1388.6505 1388.6493 0.87 0 94 3.1e-06 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1857 345 – 357 695.3326 1388.6507 1388.6493 0.96 0 60 0.0086 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1858 345 – 357 695.3326 1388.6507 1388.6493 0.96 0 83 4.2e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1860 345 – 357 695.3329 1388.6513 1388.6493 1.39 0 82 5.1e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1861 345 – 357 695.3330 1388.6514 1388.6493 1.49 0 84 3.6e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1862 345 – 357 695.3331 1388.6516 1388.6493 1.61 0 82 4.9e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1863 345 – 357 695.3331 1388.6516 1388.6493 1.63 0 53 0.041 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1864 345 – 357 695.3331 1388.6517 1388.6493 1.71 0 83 3.8e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1866 345 – 357 695.3332 1388.6518 1388.6493 1.75 0 82 5.6e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1868 345 – 357 695.3337 1388.6529 1388.6493 2.56 0 82 5.2e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1885 345 – 357 698.3241 1394.6337 1394.6364 -1.91 0 68 1 U K.TVEASPPDGMELK.K + [+21.9819 at D8] 1930 345 – 357 708.8381 1415.6616 1415.6602 0.94 0 62 1 U K.TVEASPPDGMELK.K + [+43.0058 at M10] 1931 345 – 357 708.8387 1415.6628 1415.6602 1.85 0 66 1 U K.TVEASPPDGMELK.K + [+43.0058 at M10] 1932 345 – 357 708.8393 1415.6640 1415.6602 2.68 0 87 1 U K.TVEASPPDGMELK.K + [+43.0058 at M10] 1951 345 – 357 477.5667 1429.6782 1429.6759 1.63 0 55 1 U K.TVEASPPDGMELK.K + Oxidation (M); [+41.0266 at N-term] 1954 345 – 357 715.8467 1429.6789 1429.7123 -23.4 0 67 1 U K.TVEASPPDGMELK.K + Oxidation (M); [+41.0629 at S5] 2115 345 – 358 506.5892 1516.7459 1516.7443 1.07 1 60 0.007 1 U K.TVEASPPDGMELKK.W + Oxidation (M)

2) ERROR TOLERANT search for ZmSGT1 in presence of MEK2KR and in absence of SIPK and See1

MASCOT Search Results

Protein View: gi|670432243PREDICTED: protein SGT1 homolog [Zea mays]





Search parameters




1 MAASDLESKA KEAFVDDDFE LAAELYTQAI DAGPATADLY ADRAQAHIKL51 GNYTEAVADA NKAIGLDPTM HKAYYRKGAA CIKLEEYQTA KAALELGSSY101 APGDSRFTRL LKECDECIAE ESSQAPAKNV EAPVAATVED KEDVANMDNT151 PPVVEPPSKP KYRHDYYNSA TEVVLTIYAK GVPADSVVID FGDQMLSVSI201 EVPGEEPYHF QPRLFSKIIP EKCKYQVLST KVEIRLAKAE QVTWTTLDYS251 GRPKAIPQKI STPAETAPRP SYPSSKSKKD WDKLEAEVKK EEKEEKLEGD301 AALNKFFRDI YKDADEDMRR AMDKSFRESN GTVLSTNWKD VGSKTVEASP351 PDGMELKKWE I



Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 241 2 – 9 431.7113 861.4080 861.4192 -12.9 0 67 1 M.AASDLESK.A + [+42.0218 at N-term] 244 2 – 9 431.7118 861.4090 861.4192 -11.8 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 246 2 – 9 431.7119 861.4091 861.4192 -11.6 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 247 2 – 9 431.7120 861.4094 861.4192 -11.4 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 248 2 – 9 431.7120 861.4094 861.4192 -11.4 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 249 2 – 9 431.7120 861.4094 861.4192 -11.4 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 250 2 – 9 431.7120 861.4094 861.4192 -11.3 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 251 2 – 9 431.7121 861.4096 861.4192 -11.1 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 252 2 – 9 431.7122 861.4099 861.4192 -10.8 0 76 1 M.AASDLESK.A + [+42.0218 at N-term] 255 2 – 9 431.7123 861.4101 861.4192 -10.5 0 80 1 M.AASDLESK.A + [+42.0218 at N-term] 44 3 – 9 375.1875 748.3604 748.3603 0.20 0 58 1 A.ASDLESK.A 45 3 – 9 375.1876 748.3607 748.3603 0.53 0 56 1 A.ASDLESK.A 46 3 – 9 375.1877 748.3609 748.3603 0.85 0 55 1 A.ASDLESK.A 47 3 – 9 375.1878 748.3611 748.3603 1.14 0 57 1 A.ASDLESK.A 48 3 – 9 375.1879 748.3612 748.3603 1.19 0 54 1 A.ASDLESK.A 49 3 – 9 375.1879 748.3612 748.3603 1.22 0 55 1 A.ASDLESK.A 50 3 – 9 375.1881 748.3616 748.3603 1.73 0 55 1 A.ASDLESK.A 3022 12 – 28 988.4420 1974.8693 1974.8735 -2.08 0 70 1 K.EAFVDDDFELAAELYTQ.A 3023 12 – 28 988.4421 1974.8697 1974.8735 -1.90 0 63 1 K.EAFVDDDFELAAELYTQ.A 3460 12 – 43 869.9049 3475.5904 3475.5943 -1.13 0 188 1.6e-15 1 K.EAFVDDDFELAAELYTQAIDAGPATADLYADR.A 3463 12 – 43 1159.5391 3475.5955 3475.5943 0.33 0 132 7.2e-10 1 K.EAFVDDDFELAAELYTQAIDAGPATADLYADR.A 3962 12 – 43 886.3809 3541.4944 3541.5450 -14.3 0 152 1 K.EAFVDDDFELAAELYTQAIDAGPATADLYADR.A + Phospho (ST); [-14.0157 3160 23 – 43 742.3633 2224.0680 2224.0648 1.46 0 125 1 A.AELYTQAIDAGPATADLYADR.A 3161 23 – 43 742.3635 2224.0687 2224.0648 1.79 0 108 1 A.AELYTQAIDAGPATADLYADR.A 3162 23 – 43 1113.0436 2224.0726 2224.0648 3.55 0 89 1 A.AELYTQAIDAGPATADLYADR.A 3060 25 – 43 1013.0002 2023.9858 2023.9851 0.39 0 56 1 E.LYTQAIDAGPATADLYADR.A 3061 25 – 43 675.6700 2023.9881 2023.9851 1.49 0 69 1 E.LYTQAIDAGPATADLYADR.A 3062 25 – 43 1013.0017 2023.9888 2023.9851 1.87 0 79 1 E.LYTQAIDAGPATADLYADR.A 3063 25 – 43 675.6709 2023.9910 2023.9851 2.94 0 92 1 E.LYTQAIDAGPATADLYADR.A 3064 25 – 43 675.6720 2023.9943 2023.9851 4.55 0 76 1 E.LYTQAIDAGPATADLYADR.A 2667 27 – 43 874.9263 1747.8381 1747.8377 0.26 0 142 1 Y.TQAIDAGPATADLYADR.A 2668 27 – 43 583.6200 1747.8383 1747.8377 0.35 0 83 1 Y.TQAIDAGPATADLYADR.A 2669 27 – 43 583.6202 1747.8388 1747.8377 0.63 0 88 1 Y.TQAIDAGPATADLYADR.A 2670 27 – 43 583.6204 1747.8394 1747.8377 1.00 0 85 1 Y.TQAIDAGPATADLYADR.A 2671 27 – 43 583.6205 1747.8395 1747.8377 1.06 0 82 1 Y.TQAIDAGPATADLYADR.A 2672 27 – 43 583.6205 1747.8398 1747.8377 1.21 0 94 1 Y.TQAIDAGPATADLYADR.A 2673 27 – 43 874.9272 1747.8399 1747.8377 1.30 0 145 1 Y.TQAIDAGPATADLYADR.A 2674 27 – 43 874.9278 1747.8411 1747.8377 1.94 0 145 1 Y.TQAIDAGPATADLYADR.A 2675 27 – 43 874.9280 1747.8414 1747.8377 2.12 0 142 1 Y.TQAIDAGPATADLYADR.A 2676 27 – 43 874.9280 1747.8415 1747.8377 2.19 0 145 1 Y.TQAIDAGPATADLYADR.A 2507 28 – 43 824.4042 1646.7938 1646.7900 2.32 0 123 1 T.QAIDAGPATADLYADR.A

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2196 29 – 43 760.3719 1518.7293 1518.7314 -1.40 0 108 1 Q.AIDAGPATADLYADR.A 2197 29 – 43 760.3720 1518.7295 1518.7314 -1.24 0 105 1 Q.AIDAGPATADLYADR.A 2198 29 – 43 507.2506 1518.7301 1518.7314 -0.87 0 84 1 Q.AIDAGPATADLYADR.A 2199 29 – 43 760.3723 1518.7301 1518.7314 -0.83 0 125 1 Q.AIDAGPATADLYADR.A 2200 29 – 43 760.3731 1518.7317 1518.7314 0.18 0 121 1 Q.AIDAGPATADLYADR.A 2201 29 – 43 507.2513 1518.7322 1518.7314 0.49 0 53 1 Q.AIDAGPATADLYADR.A 2202 29 – 43 507.2514 1518.7324 1518.7314 0.65 0 105 1 Q.AIDAGPATADLYADR.A 2203 29 – 43 760.3737 1518.7329 1518.7314 1.00 0 126 1 Q.AIDAGPATADLYADR.A 2204 29 – 43 760.3750 1518.7355 1518.7314 2.67 0 99 1 Q.AIDAGPATADLYADR.A 2205 29 – 43 760.3754 1518.7363 1518.7314 3.23 0 105 1 Q.AIDAGPATADLYADR.A 1541 31 – 43 668.3131 1334.6117 1334.6102 1.10 0 73 1 I.DAGPATADLYADR.A 1586 50 – 62 674.8221 1347.6297 1347.6670 -27.7 0 80 1 K.LGNYTEAVADANK.A + [-16.9902 at N12] 1770 50 – 62 683.3357 1364.6569 1364.6572 -0.21 0 97 1.8e-06 1 K.LGNYTEAVADANK.A 1771 50 – 62 683.3359 1364.6571 1364.6572 -0.024 0 93 4.2e-06 1 K.LGNYTEAVADANK.A 1772 50 – 62 683.3366 1364.6587 1364.6572 1.12 0 90 8.4e-06 1 K.LGNYTEAVADANK.A 1773 50 – 62 683.3370 1364.6594 1364.6572 1.60 0 86 1.9e-05 1 K.LGNYTEAVADANK.A 1774 50 – 62 683.3373 1364.6601 1364.6572 2.14 0 87 1.8e-05 1 K.LGNYTEAVADANK.A 1784 50 – 62 683.8279 1365.6413 1365.6542 -9.48 0 89 1 K.LGNYTEAVADANK.A + [+0.9970 at G2] 1780 50 – 62 683.8273 1365.6400 1365.6605 -15.0 0 113 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1781 50 – 62 683.8276 1365.6406 1365.6605 -14.6 0 113 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1782 50 – 62 683.8278 1365.6411 1365.6605 -14.2 0 96 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1783 50 – 62 683.8279 1365.6411 1365.6605 -14.2 0 109 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1785 50 – 62 683.8280 1365.6415 1365.6605 -13.9 0 101 1 K.LGNYTEAVADANK.A + [+1.0034 at N12] 1786 50 – 62 683.8283 1365.6419 1365.6605 -13.6 0 103 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1787 50 – 62 683.8285 1365.6425 1365.6605 -13.2 0 104 1 K.LGNYTEAVADANK.A + [+1.0034 at N3] 1926 50 – 62 696.3447 1390.6748 1390.6728 1.41 0 86 1 K.LGNYTEAVADANK.A + [+26.0156 at N-term] 717 63 – 72 541.7861 1081.5576 1081.5590 -1.29 0 63 0.003 1 U K.AIGLDPTMHK.A 718 63 – 72 361.5266 1081.5579 1081.5590 -1.01 0 66 0.0016 1 U K.AIGLDPTMHK.A 719 63 – 72 361.5271 1081.5594 1081.5590 0.38 0 66 0.0015 1 U K.AIGLDPTMHK.A 963 82 – 91 408.2283 1221.6630 1221.6605 2.09 1 73 1 C.IKLEEYQTAK.A 473 84 – 91 491.2491 980.4837 980.4814 2.31 0 57 0.017 1 K.LEEYQTAK.A 474 84 – 91 491.2492 980.4839 980.4814 2.49 0 54 0.027 1 K.LEEYQTAK.A 475 84 – 91 491.2493 980.4840 980.4814 2.59 0 52 0.046 1 K.LEEYQTAK.A 525 84 – 91 499.2460 996.4774 996.4764 1.03 0 57 1 K.LEEYQTAK.A + [+15.9949 at Y4] 2149 92 – 106 747.3635 1492.7125 1492.7158 -2.18 0 83 4.8e-05 1 U K.AALELGSSYAPGDSR.F 2150 92 – 106 747.3638 1492.7130 1492.7158 -1.82 0 99 1e-06 1 U K.AALELGSSYAPGDSR.F 2151 92 – 106 747.3638 1492.7131 1492.7158 -1.79 0 81 7.3e-05 1 U K.AALELGSSYAPGDSR.F 2152 92 – 106 747.3639 1492.7132 1492.7158 -1.68 0 120 8.6e-09 1 U K.AALELGSSYAPGDSR.F 2153 92 – 106 498.5785 1492.7136 1492.7158 -1.48 0 100 8.9e-07 1 U K.AALELGSSYAPGDSR.F 2154 92 – 106 747.3641 1492.7137 1492.7158 -1.35 0 83 4.1e-05 1 U K.AALELGSSYAPGDSR.F 2155 92 – 106 498.5786 1492.7140 1492.7158 -1.20 0 101 6.1e-07 1 U K.AALELGSSYAPGDSR.F 2156 92 – 106 747.3646 1492.7147 1492.7158 -0.70 0 95 3e-06 1 U K.AALELGSSYAPGDSR.F 2157 92 – 106 747.3647 1492.7149 1492.7158 -0.56 0 72 0.00054 1 U K.AALELGSSYAPGDSR.F 2158 92 – 106 747.3654 1492.7163 1492.7158 0.34 0 79 0.0001 1 U K.AALELGSSYAPGDSR.F 2160 92 – 106 747.3665 1492.7184 1492.7158 1.79 0 101 6.3e-07 1 U K.AALELGSSYAPGDSR.F 2164 92 – 106 747.3688 1492.7230 1492.7158 4.84 0 69 0.0011 1 U K.AALELGSSYAPGDSR.F 2167 92 – 106 747.8669 1493.7192 1493.7128 4.29 0 85 1 U K.AALELGSSYAPGDSR.F + [+0.9970 at N-term A]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2357 92 – 106 776.3653 1550.7160 1550.7212 -3.35 0 105 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 2358 92 – 106 776.3666 1550.7187 1550.7212 -1.64 0 112 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 2359 92 – 106 776.3680 1550.7215 1550.7212 0.17 0 105 1 U K.AALELGSSYAPGDSR.F + [+58.0055 at G12] 3103 110 – 128 720.6911 2159.0516 2159.0238 12.8 1 52 1 U R.LLKECDECIAEESSQAPAK.N + [-17.9742 at D6] 3104 110 – 128 540.7706 2159.0535 2159.0238 13.7 1 70 5 U R.LLKECDECIAEESSQAPAK.N + [-17.9742 at D6] 3105 110 – 128 720.6919 2159.0540 2159.0238 14.0 1 64 1 U R.LLKECDECIAEESSQAPAK.N + [-17.9742 at D6] 3107 110 – 128 720.6929 2159.0569 2159.0238 15.3 1 73 1 U R.LLKECDECIAEESSQAPAK.N + [-17.9742 at D6] 1566 129 – 141 671.8459 1341.6772 1341.6776 -0.30 0 90 6e-06 1 U K.NVEAPVAATVEDK.E 1567 129 – 141 671.8460 1341.6774 1341.6776 -0.14 0 90 6e-06 1 U K.NVEAPVAATVEDK.E 1568 129 – 141 671.8462 1341.6778 1341.6776 0.16 0 90 6e-06 1 U K.NVEAPVAATVEDK.E 1569 129 – 141 671.8463 1341.6780 1341.6776 0.29 0 90 6.1e-06 1 U K.NVEAPVAATVEDK.E 1575 129 – 141 672.3386 1342.6627 1342.6746 -8.87 0 99 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2] 1576 129 – 141 672.3387 1342.6629 1342.6746 -8.74 0 96 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2] 1577 129 – 141 672.3394 1342.6643 1342.6746 -7.72 0 91 1 U K.NVEAPVAATVEDK.E + [+0.9970 at V2] 2424 129 – 143 793.8814 1585.7482 1585.7471 0.69 1 74 1 U K.NVEAPVAATVEDKED.V 2425 129 – 143 793.8822 1585.7497 1585.7471 1.65 1 52 1 U K.NVEAPVAATVEDKED.V 2909 129 – 146 935.9535 1869.8925 1869.8956 -1.67 1 52 1 U K.NVEAPVAATVEDKEDVAN.M 3365 129 – 159 816.3947 3261.5496 3261.5711 -6.60 1 67 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3366 129 – 159 816.3984 3261.5646 3261.5711 -2.00 1 85 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3368 129 – 159 816.3994 3261.5686 3261.5711 -0.76 1 92 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3369 129 – 159 816.3995 3261.5691 3261.5711 -0.62 1 86 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3370 129 – 159 1088.1970 3261.5692 3261.5711 -0.59 1 148 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3371 129 – 159 816.3996 3261.5693 3261.5711 -0.55 1 95 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3372 129 – 159 1088.1976 3261.5710 3261.5711 -0.036 1 145 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3373 129 – 159 653.3215 3261.5711 3261.5711 0.0089 1 89 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3374 129 – 159 1088.1979 3261.5719 3261.5711 0.24 1 161 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3375 129 – 159 816.4003 3261.5721 3261.5711 0.30 1 80 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3376 129 – 159 816.4004 3261.5723 3261.5711 0.37 1 76 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3377 129 – 159 1088.1983 3261.5731 3261.5711 0.61 1 139 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3378 129 – 159 1088.1987 3261.5743 3261.5711 0.98 1 155 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3379 129 – 159 1088.1990 3261.5752 3261.5711 1.25 1 158 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3380 129 – 159 1088.1992 3261.5758 3261.5711 1.44 1 126 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3381 129 – 159 1088.1992 3261.5758 3261.5711 1.44 1 139 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P 3396 129 – 159 820.3985 3277.5649 3277.5660 -0.33 1 59 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3397 129 – 159 1093.5311 3277.5715 3277.5660 1.67 1 142 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3398 129 – 159 820.4002 3277.5717 3277.5660 1.72 1 75 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3399 129 – 159 1093.5319 3277.5739 3277.5660 2.40 1 168 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSK.P + Oxidation (M) 3472 129 – 161 1163.2412 3486.7018 3486.7188 -4.89 1 156 1.7e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3473 129 – 161 1163.2418 3486.7036 3486.7188 -4.37 1 164 3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3474 129 – 161 872.6840 3486.7069 3486.7188 -3.43 1 181 5.8e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3475 129 – 161 1163.2437 3486.7093 3486.7188 -2.74 1 180 7.1e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3476 129 – 161 872.6850 3486.7107 3486.7188 -2.32 1 116 1.7e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3477 129 – 161 698.3494 3486.7108 3486.7188 -2.31 1 137 1.3e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3478 129 – 161 872.6851 3486.7113 3486.7188 -2.14 1 102 4.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3479 129 – 161 698.3496 3486.7114 3486.7188 -2.12 1 153 3.5e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3480 129 – 161 698.3496 3486.7114 3486.7188 -2.12 1 153 3.6e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3481 129 – 161 698.3496 3486.7117 3486.7188 -2.03 1 132 4.6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3482 129 – 161 698.3496 3486.7118 3486.7188 -2.02 1 162 4.3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3483 129 – 161 698.3496 3486.7118 3486.7188 -2.01 1 139 8.6e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3484 129 – 161 1163.2446 3486.7120 3486.7188 -1.96 1 181 5.6e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3486 129 – 161 698.3497 3486.7121 3486.7188 -1.92 1 168 1e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3487 129 – 161 1163.2447 3486.7123 3486.7188 -1.88 1 158 9.7e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3488 129 – 161 698.3497 3486.7124 3486.7188 -1.85 1 143 3.1e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3489 129 – 161 698.3498 3486.7125 3486.7188 -1.80 1 156 1.6e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3490 129 – 161 698.3498 3486.7126 3486.7188 -1.79 1 156 1.8e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3491 129 – 161 698.3498 3486.7126 3486.7188 -1.78 1 120 7.1e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3493 129 – 161 872.6855 3486.7129 3486.7188 -1.71 1 140 7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3494 129 – 161 698.3499 3486.7130 3486.7188 -1.68 1 156 1.5e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3495 129 – 161 698.3499 3486.7130 3486.7188 -1.66 1 163 3.3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3496 129 – 161 872.6856 3486.7131 3486.7188 -1.63 1 69 0.00075 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3497 129 – 161 698.3499 3486.7132 3486.7188 -1.62 1 176 1.5e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3498 129 – 161 582.1261 3486.7132 3486.7188 -1.61 1 99 7.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3499 129 – 161 698.3500 3486.7138 3486.7188 -1.43 1 164 2.4e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3500 129 – 161 1163.2453 3486.7141 3486.7188 -1.36 1 116 1.7e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3502 129 – 161 872.6859 3486.7143 3486.7188 -1.28 1 136 1.7e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3503 129 – 161 1163.2454 3486.7144 3486.7188 -1.27 1 172 4.4e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3504 129 – 161 872.6859 3486.7145 3486.7188 -1.23 1 107 1.2e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3505 129 – 161 582.1264 3486.7146 3486.7188 -1.22 1 120 6.1e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3506 129 – 161 872.6860 3486.7149 3486.7188 -1.12 1 74 0.00024 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3507 129 – 161 872.6860 3486.7149 3486.7188 -1.11 1 126 1.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3508 129 – 161 872.6860 3486.7151 3486.7188 -1.08 1 131 5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3509 129 – 161 872.6860 3486.7151 3486.7188 -1.08 1 159 8e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3510 129 – 161 872.6861 3486.7151 3486.7188 -1.05 1 126 1.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3511 129 – 161 872.6861 3486.7151 3486.7188 -1.05 1 134 2.3e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3512 129 – 161 872.6861 3486.7153 3486.7188 -1.01 1 120 6.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3513 129 – 161 872.6861 3486.7155 3486.7188 -0.95 1 129 7.4e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3516 129 – 161 872.6862 3486.7157 3486.7188 -0.90 1 125 2e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3518 129 – 161 698.3504 3486.7157 3486.7188 -0.89 1 151 5.1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3519 129 – 161 872.6862 3486.7157 3486.7188 -0.88 1 150 6e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3521 129 – 161 698.3505 3486.7159 3486.7188 -0.84 1 158 1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3522 129 – 161 872.6862 3486.7159 3486.7188 -0.84 1 134 2.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3523 129 – 161 698.3505 3486.7159 3486.7188 -0.83 1 151 5.6e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3525 129 – 161 698.3505 3486.7160 3486.7188 -0.81 1 159 7.6e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3526 129 – 161 872.6863 3486.7160 3486.7188 -0.81 1 90 6.8e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3527 129 – 161 1163.2460 3486.7162 3486.7188 -0.76 1 189 7.9e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3528 129 – 161 872.6863 3486.7162 3486.7188 -0.74 1 138 9.7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3529 129 – 161 872.6864 3486.7164 3486.7188 -0.69 1 138 9.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3530 129 – 161 698.3506 3486.7164 3486.7188 -0.69 1 177 1.4e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3531 129 – 161 872.6864 3486.7165 3486.7188 -0.68 1 160 5.6e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3533 129 – 161 872.6864 3486.7165 3486.7188 -0.65 1 103 3e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3534 129 – 161 872.6864 3486.7166 3486.7188 -0.64 1 129 7.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3535 129 – 161 872.6864 3486.7166 3486.7188 -0.63 1 138 9.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3537 129 – 161 872.6865 3486.7169 3486.7188 -0.54 1 104 2.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3538 129 – 161 872.6865 3486.7169 3486.7188 -0.54 1 82 3.8e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3539 129 – 161 872.6865 3486.7169 3486.7188 -0.54 1 151 5.5e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3540 129 – 161 872.6865 3486.7171 3486.7188 -0.50 1 146 1.5e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3541 129 – 161 872.6865 3486.7171 3486.7188 -0.50 1 127 1.4e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3542 129 – 161 872.6866 3486.7171 3486.7188 -0.49 1 129 7.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3543 129 – 161 872.6866 3486.7174 3486.7188 -0.41 1 104 2.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3544 129 – 161 872.6867 3486.7177 3486.7188 -0.33 1 104 2.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3547 129 – 161 1163.2466 3486.7180 3486.7188 -0.24 1 139 7.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3548 129 – 161 1163.2466 3486.7180 3486.7188 -0.24 1 147 1.3e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3549 129 – 161 872.6868 3486.7181 3486.7188 -0.21 1 95 2.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3550 129 – 161 872.6868 3486.7182 3486.7188 -0.17 1 122 4.4e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3551 129 – 161 872.6868 3486.7183 3486.7188 -0.16 1 119 7.7e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3552 129 – 161 1163.2467 3486.7183 3486.7188 -0.15 1 164 2.3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3553 129 – 161 872.6869 3486.7185 3486.7188 -0.10 1 135 1.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3555 129 – 161 872.6869 3486.7186 3486.7188 -0.067 1 172 4.4e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3556 129 – 161 872.6870 3486.7188 3486.7188 -0.010 1 139 7e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3557 129 – 161 872.6870 3486.7188 3486.7188 0.0014 1 123 2.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3558 129 – 161 698.3510 3486.7188 3486.7188 0.0026 1 52 0.043 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3563 129 – 161 872.6871 3486.7194 3486.7188 0.16 1 176 1.7e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3564 129 – 161 872.6872 3486.7196 3486.7188 0.23 1 157 1.4e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3565 129 – 161 698.3512 3486.7197 3486.7188 0.26 1 158 9.1e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3566 129 – 161 872.6872 3486.7198 3486.7188 0.29 1 115 1.9e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3568 129 – 161 872.6873 3486.7200 3486.7188 0.33 1 119 7.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3569 129 – 161 698.3513 3486.7201 3486.7188 0.38 1 63 0.0029 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3571 129 – 161 872.6873 3486.7202 3486.7188 0.40 1 102 3.5e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3572 129 – 161 872.6873 3486.7203 3486.7188 0.41 1 112 3.6e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3573 129 – 161 872.6874 3486.7203 3486.7188 0.43 1 111 4.7e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3575 129 – 161 872.6874 3486.7203 3486.7188 0.44 1 115 1.9e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3577 129 – 161 872.6874 3486.7205 3486.7188 0.48 1 110 6.5e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3578 129 – 161 872.6874 3486.7206 3486.7188 0.51 1 93 3.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3579 129 – 161 698.3514 3486.7207 3486.7188 0.53 1 135 1.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3580 129 – 161 1163.2475 3486.7207 3486.7188 0.53 1 135 1.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3581 129 – 161 1163.2475 3486.7207 3486.7188 0.53 1 183 2.8e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3582 129 – 161 872.6875 3486.7208 3486.7188 0.56 1 157 1.1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3584 129 – 161 1163.2476 3486.7210 3486.7188 0.62 1 75 0.00017 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3585 129 – 161 872.6875 3486.7210 3486.7188 0.63 1 114 2.2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3587 129 – 161 872.6875 3486.7211 3486.7188 0.66 1 103 3e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3588 129 – 161 872.6876 3486.7211 3486.7188 0.67 1 76 0.00014 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3589 129 – 161 872.6876 3486.7213 3486.7188 0.71 1 80 5.9e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3590 129 – 161 872.6876 3486.7215 3486.7188 0.77 1 106 1.4e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3591 129 – 161 872.6877 3486.7215 3486.7188 0.78 1 89 6.9e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3592 129 – 161 872.6877 3486.7215 3486.7188 0.78 1 97 1.3e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3593 129 – 161 872.6877 3486.7217 3486.7188 0.82 1 126 1.4e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3594 129 – 161 872.6877 3486.7217 3486.7188 0.83 1 91 5.1e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3596 129 – 161 872.6877 3486.7217 3486.7188 0.84 1 83 3.2e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3597 129 – 161 872.6877 3486.7218 3486.7188 0.86 1 100 5.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3598 129 – 161 872.6877 3486.7219 3486.7188 0.87 1 146 1.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3602 129 – 161 872.6878 3486.7221 3486.7188 0.93 1 84 2.6e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3603 129 – 161 872.6878 3486.7221 3486.7188 0.94 1 85 2e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3604 129 – 161 872.6878 3486.7222 3486.7188 0.98 1 138 9e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3605 129 – 161 872.6878 3486.7223 3486.7188 0.99 1 100 5.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3606 129 – 161 872.6878 3486.7223 3486.7188 0.99 1 147 1.3e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3608 129 – 161 872.6879 3486.7223 3486.7188 1.00 1 79 6.9e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3609 129 – 161 872.6879 3486.7223 3486.7188 1.00 1 97 1.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3610 129 – 161 872.6879 3486.7223 3486.7188 1.01 1 82 3.8e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3611 129 – 161 872.6879 3486.7223 3486.7188 1.01 1 72 0.0004 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3613 129 – 161 872.6879 3486.7225 3486.7188 1.05 1 120 6.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3614 129 – 161 872.6879 3486.7225 3486.7188 1.06 1 104 2.7e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3615 129 – 161 698.3518 3486.7227 3486.7188 1.12 1 81 4.5e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3616 129 – 161 872.6880 3486.7227 3486.7188 1.13 1 69 0.00073 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3617 129 – 161 872.6880 3486.7227 3486.7188 1.13 1 136 1.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3619 129 – 161 872.6880 3486.7228 3486.7188 1.15 1 107 1.2e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3620 129 – 161 698.3518 3486.7228 3486.7188 1.15 1 163 3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3621 129 – 161 872.6880 3486.7229 3486.7188 1.16 1 74 0.00024 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3622 129 – 161 872.6880 3486.7229 3486.7188 1.16 1 147 1.2e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3623 129 – 161 872.6880 3486.7229 3486.7188 1.17 1 92 3.5e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3624 129 – 161 872.6880 3486.7229 3486.7188 1.17 1 106 1.5e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3625 129 – 161 872.6880 3486.7231 3486.7188 1.23 1 56 0.015 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3626 129 – 161 872.6881 3486.7231 3486.7188 1.24 1 95 1.9e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3627 129 – 161 872.6881 3486.7232 3486.7188 1.26 1 104 2.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3628 129 – 161 872.6881 3486.7233 3486.7188 1.27 1 143 3e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3629 129 – 161 698.3519 3486.7233 3486.7188 1.28 1 177 1.3e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3630 129 – 161 872.6881 3486.7233 3486.7188 1.29 1 112 4e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3631 129 – 161 698.3519 3486.7233 3486.7188 1.29 1 75 0.00019 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3632 129 – 161 872.6881 3486.7233 3486.7188 1.30 1 87 1.2e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3633 129 – 161 872.6881 3486.7234 3486.7188 1.31 1 124 2.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3634 129 – 161 872.6881 3486.7234 3486.7188 1.32 1 88 1e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3635 129 – 161 872.6881 3486.7234 3486.7188 1.32 1 98 9.3e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3636 129 – 161 872.6881 3486.7235 3486.7188 1.33 1 121 5.1e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3637 129 – 161 872.6881 3486.7235 3486.7188 1.33 1 72 0.00038 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3638 129 – 161 872.6882 3486.7236 3486.7188 1.37 1 106 1.4e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3639 129 – 161 872.6882 3486.7237 3486.7188 1.40 1 95 2.1e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3640 129 – 161 872.6882 3486.7237 3486.7188 1.41 1 85 1.9e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3641 129 – 161 872.6882 3486.7239 3486.7188 1.45 1 98 8.7e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3642 129 – 161 872.6883 3486.7239 3486.7188 1.46 1 150 6.1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3643 129 – 161 1163.2486 3486.7240 3486.7188 1.48 1 202 3.8e-17 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3645 129 – 161 872.6883 3486.7241 3486.7188 1.53 1 85 1.9e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3646 129 – 161 872.6883 3486.7241 3486.7188 1.53 1 95 1.8e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3647 129 – 161 872.6883 3486.7241 3486.7188 1.53 1 96 1.4e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3648 129 – 161 872.6883 3486.7242 3486.7188 1.54 1 163 3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3649 129 – 161 872.6883 3486.7242 3486.7188 1.55 1 73 0.00033 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3650 129 – 161 872.6883 3486.7243 3486.7188 1.56 1 136 1.5e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3651 129 – 161 872.6884 3486.7245 3486.7188 1.64 1 94 2.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3652 129 – 161 1163.2488 3486.7246 3486.7188 1.65 1 163 3.2e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3653 129 – 161 872.6884 3486.7246 3486.7188 1.66 1 115 2e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3654 129 – 161 872.6885 3486.7248 3486.7188 1.72 1 146 1.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3655 129 – 161 1163.2490 3486.7252 3486.7188 1.82 1 208 8.5e-18 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3656 129 – 161 872.6886 3486.7252 3486.7188 1.83 1 95 2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3657 129 – 161 872.6886 3486.7252 3486.7188 1.83 1 145 1.9e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3658 129 – 161 872.6886 3486.7253 3486.7188 1.87 1 121 4.7e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3659 129 – 161 872.6886 3486.7253 3486.7188 1.87 1 152 3.6e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3660 129 – 161 872.6887 3486.7255 3486.7188 1.93 1 124 2.4e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3661 129 – 161 872.6887 3486.7255 3486.7188 1.93 1 128 9.4e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3662 129 – 161 872.6887 3486.7255 3486.7188 1.93 1 148 9.2e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3663 129 – 161 872.6887 3486.7256 3486.7188 1.94 1 111 5.3e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3664 129 – 161 872.6887 3486.7258 3486.7188 2.01 1 120 5.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3665 129 – 161 872.6888 3486.7259 3486.7188 2.04 1 123 2.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3666 129 – 161 872.6888 3486.7259 3486.7188 2.04 1 115 1.7e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3667 129 – 161 1163.2493 3486.7261 3486.7188 2.08 1 164 2.3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3668 129 – 161 872.6889 3486.7263 3486.7188 2.16 1 108 9.6e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3669 129 – 161 872.6889 3486.7265 3486.7188 2.22 1 122 3.3e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3670 129 – 161 872.6889 3486.7267 3486.7188 2.25 1 131 4.6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3672 129 – 161 872.6890 3486.7268 3486.7188 2.28 1 115 1.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3673 129 – 161 872.6890 3486.7270 3486.7188 2.35 1 88 9.4e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3676 129 – 161 872.6891 3486.7274 3486.7188 2.46 1 85 1.8e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3677 129 – 161 1163.2498 3486.7276 3486.7188 2.51 1 161 4.9e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3679 129 – 161 1163.2500 3486.7282 3486.7188 2.68 1 174 2.2e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3680 129 – 161 1163.2501 3486.7285 3486.7188 2.77 1 179 6.9e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3682 129 – 161 1163.2502 3486.7288 3486.7188 2.86 1 147 1e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3683 129 – 161 1163.2502 3486.7288 3486.7188 2.86 1 167 1.1e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3684 129 – 161 1163.2502 3486.7288 3486.7188 2.86 1 217 1.1e-18 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3685 129 – 161 872.6896 3486.7294 3486.7188 3.03 1 90 6.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3687 129 – 161 872.6898 3486.7302 3486.7188 3.27 1 117 1.1e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3688 129 – 161 1163.2507 3486.7303 3486.7188 3.29 1 197 1.1e-16 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3690 129 – 161 1163.2511 3486.7315 3486.7188 3.63 1 176 1.3e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3693 129 – 161 1163.2515 3486.7327 3486.7188 3.98 1 60 0.0062 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3695 129 – 161 1163.2517 3486.7333 3486.7188 4.15 1 157 1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3699 129 – 161 1163.2520 3486.7342 3486.7188 4.41 1 199 6.8e-17 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3702 129 – 161 1163.2522 3486.7348 3486.7188 4.58 1 212 3.7e-18 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3705 129 – 161 1163.2527 3486.7363 3486.7188 5.01 1 62 0.0031 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3711 129 – 161 1163.2557 3486.7453 3486.7188 7.59 1 213 2.5e-18 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3712 129 – 161 1163.2608 3486.7606 3486.7188 12.0 1 79 5.5e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y 3747 129 – 161 876.6829 3502.7025 3502.7137 -3.20 1 56 0.017 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3748 129 – 161 876.6838 3502.7059 3502.7137 -2.23 1 114 3e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3749 129 – 161 876.6838 3502.7060 3502.7137 -2.20 1 121 5.7e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3750 129 – 161 876.6838 3502.7061 3502.7137 -2.17 1 92 4.5e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3751 129 – 161 701.5486 3502.7065 3502.7137 -2.07 1 136 1.7e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3753 129 – 161 876.6839 3502.7067 3502.7137 -2.02 1 150 6.4e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3754 129 – 161 701.5487 3502.7070 3502.7137 -1.92 1 142 4.2e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3755 129 – 161 1168.5763 3502.7071 3502.7137 -1.90 1 138 1.2e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3757 129 – 161 876.6842 3502.7076 3502.7137 -1.76 1 108 1.2e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3758 129 – 161 701.5488 3502.7077 3502.7137 -1.72 1 159 8.2e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3759 129 – 161 701.5489 3502.7080 3502.7137 -1.63 1 159 8.4e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3760 129 – 161 876.6843 3502.7082 3502.7137 -1.57 1 106 1.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3761 129 – 161 1168.5767 3502.7083 3502.7137 -1.56 1 159 8.7e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3763 129 – 161 701.5490 3502.7085 3502.7137 -1.50 1 164 3e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3764 129 – 161 876.6844 3502.7086 3502.7137 -1.46 1 127 1.5e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3765 129 – 161 876.6845 3502.7087 3502.7137 -1.43 1 88 1.1e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3767 129 – 161 584.7921 3502.7092 3502.7137 -1.28 1 72 0.00049 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3768 129 – 161 701.5491 3502.7093 3502.7137 -1.26 1 158 1e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3769 129 – 161 876.6846 3502.7093 3502.7137 -1.25 1 142 4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3770 129 – 161 584.7922 3502.7094 3502.7137 -1.23 1 77 0.00015 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3772 129 – 161 876.6847 3502.7096 3502.7137 -1.17 1 112 4.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3773 129 – 161 876.6847 3502.7099 3502.7137 -1.11 1 143 3.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3774 129 – 161 701.5493 3502.7100 3502.7137 -1.06 1 132 4.8e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3775 129 – 161 701.5493 3502.7101 3502.7137 -1.04 1 175 2.4e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3776 129 – 161 876.6849 3502.7104 3502.7137 -0.96 1 105 2e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3777 129 – 161 876.6849 3502.7105 3502.7137 -0.92 1 98 1.2e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3778 129 – 161 876.6850 3502.7108 3502.7137 -0.84 1 120 6.8e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3779 129 – 161 701.5494 3502.7108 3502.7137 -0.83 1 96 1.8e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3780 129 – 161 876.6850 3502.7109 3502.7137 -0.80 1 137 1.3e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3781 129 – 161 701.5495 3502.7110 3502.7137 -0.77 1 151 5.9e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3782 129 – 161 584.7925 3502.7110 3502.7137 -0.77 1 74 0.00026 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3783 129 – 161 1168.5778 3502.7116 3502.7137 -0.62 1 160 6.7e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3784 129 – 161 876.6852 3502.7116 3502.7137 -0.61 1 131 5.6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3785 129 – 161 876.6852 3502.7118 3502.7137 -0.56 1 106 1.8e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3787 129 – 161 876.6853 3502.7120 3502.7137 -0.50 1 112 4.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3788 129 – 161 1168.5780 3502.7122 3502.7137 -0.44 1 147 1.4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3791 129 – 161 876.6853 3502.7123 3502.7137 -0.41 1 100 6.3e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3792 129 – 161 876.6854 3502.7125 3502.7137 -0.36 1 123 3.6e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3794 129 – 161 876.6854 3502.7127 3502.7137 -0.30 1 63 0.0037 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3796 129 – 161 876.6855 3502.7130 3502.7137 -0.22 1 101 5.6e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3797 129 – 161 876.6855 3502.7130 3502.7137 -0.22 1 74 0.00026 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3800 129 – 161 1168.5784 3502.7134 3502.7137 -0.10 1 181 5.4e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3804 129 – 161 701.5501 3502.7139 3502.7137 0.040 1 149 9e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3805 129 – 161 876.6858 3502.7140 3502.7137 0.070 1 100 6.1e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3812 129 – 161 876.6859 3502.7143 3502.7137 0.17 1 57 0.014 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3817 129 – 161 876.6859 3502.7145 3502.7137 0.23 1 142 4e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3820 129 – 161 876.6860 3502.7147 3502.7137 0.28 1 64 0.0027 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3821 129 – 161 876.6860 3502.7147 3502.7137 0.29 1 148 1e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3825 129 – 161 876.6861 3502.7151 3502.7137 0.39 1 113 3.5e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3826 129 – 161 1168.5790 3502.7152 3502.7137 0.41 1 74 0.00029 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3829 129 – 161 701.5503 3502.7152 3502.7137 0.43 1 181 5.6e-15 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3834 129 – 161 876.6862 3502.7155 3502.7137 0.52 1 133 3.6e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3838 129 – 161 876.6862 3502.7158 3502.7137 0.60 1 105 2e-07 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3841 129 – 161 701.5505 3502.7162 3502.7137 0.71 1 176 1.7e-14 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3842 129 – 161 1168.5794 3502.7164 3502.7137 0.75 1 135 2.3e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3844 129 – 161 701.5506 3502.7164 3502.7137 0.77 1 122 4.1e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3847 129 – 161 876.6864 3502.7167 3502.7137 0.84 1 54 0.025 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3849 129 – 161 876.6865 3502.7169 3502.7137 0.90 1 64 0.0027 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3851 129 – 161 876.6865 3502.7169 3502.7137 0.92 1 69 0.00084 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3853 129 – 161 876.6866 3502.7171 3502.7137 0.97 1 51 0.049 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3857 129 – 161 876.6866 3502.7174 3502.7137 1.05 1 125 2.1e-09 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3858 129 – 161 876.6866 3502.7175 3502.7137 1.08 1 94 2.5e-06 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3861 129 – 161 876.6868 3502.7182 3502.7137 1.28 1 113 3.5e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3862 129 – 161 876.6869 3502.7185 3502.7137 1.35 1 66 0.0015 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3863 129 – 161 1168.5802 3502.7188 3502.7137 1.44 1 159 7.7e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3864 129 – 161 1168.5802 3502.7188 3502.7137 1.44 1 154 2.9e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3868 129 – 161 876.6872 3502.7195 3502.7137 1.66 1 52 0.041 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3871 129 – 161 1168.5809 3502.7209 3502.7137 2.04 1 160 5.8e-13 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3873 129 – 161 876.6876 3502.7212 3502.7137 2.14 1 54 0.023 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3874 129 – 161 1168.5812 3502.7218 3502.7137 2.30 1 114 2.8e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3875 129 – 161 876.6879 3502.7225 3502.7137 2.49 1 142 3.8e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3876 129 – 161 876.6879 3502.7227 3502.7137 2.55 1 135 1.9e-10 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3878 129 – 161 1168.5816 3502.7230 3502.7137 2.64 1 83 3.5e-05 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3879 129 – 161 1168.5821 3502.7245 3502.7137 3.07 1 150 6.2e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3880 129 – 161 1168.5822 3502.7248 3502.7137 3.15 1 71 0.0005 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3882 129 – 161 1168.5825 3502.7257 3502.7137 3.41 1 145 1.8e-11 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3883 129 – 161 876.6887 3502.7259 3502.7137 3.46 1 59 0.0071 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3884 129 – 161 1168.5830 3502.7272 3502.7137 3.84 1 69 0.00076 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3887 129 – 161 1168.5840 3502.7302 3502.7137 4.69 1 152 3.9e-12 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3888 129 – 161 1168.5845 3502.7317 3502.7137 5.12 1 108 9.9e-08 1 U K.NVEAPVAATVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3294 137 – 161 684.8397 2735.3297 2735.3324 -0.98 1 105 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3295 137 – 161 684.8399 2735.3306 2735.3324 -0.65 1 116 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3296 137 – 161 684.8402 2735.3316 2735.3324 -0.29 1 103 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3297 137 – 161 684.8402 2735.3319 2735.3324 -0.17 1 113 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3298 137 – 161 684.8403 2735.3322 2735.3324 -0.055 1 106 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3299 137 – 161 912.7848 2735.3326 2735.3324 0.086 1 125 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3301 137 – 161 912.7851 2735.3334 2735.3324 0.39 1 108 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3302 137 – 161 912.7852 2735.3337 2735.3324 0.48 1 126 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3303 137 – 161 912.7852 2735.3339 2735.3324 0.56 1 106 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3304 137 – 161 912.7853 2735.3340 2735.3324 0.60 1 131 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3305 137 – 161 912.7853 2735.3341 2735.3324 0.62 1 79 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y 3309 137 – 161 688.8392 2751.3279 2751.3273 0.21 1 68 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3310 137 – 161 688.8395 2751.3290 2751.3273 0.63 1 103 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3311 137 – 161 688.8397 2751.3297 2751.3273 0.88 1 136 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3312 137 – 161 918.1173 2751.3300 2751.3273 1.00 1 53 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3313 137 – 161 918.1176 2751.3309 2751.3273 1.31 1 63 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3314 137 – 161 918.1181 2751.3326 2751.3273 1.92 1 103 1 U A.TVEDKEDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 2988 142 – 159 646.9734 1937.8983 1937.9041 -2.95 0 52 1 U K.EDVANMDNTPPVVEPPSK.P 2989 142 – 159 646.9747 1937.9024 1937.9041 -0.87 0 86 1 U K.EDVANMDNTPPVVEPPSK.P 3108 142 – 161 722.0222 2163.0446 2163.0518 -3.31 0 111 6.6e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3110 142 – 161 722.0234 2163.0485 2163.0518 -1.53 0 116 2.2e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3111 142 – 161 722.0240 2163.0503 2163.0518 -0.68 0 76 0.0002 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3113 142 – 161 722.0242 2163.0507 2163.0518 -0.49 0 97 1.8e-06 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3114 142 – 161 1082.5329 2163.0512 2163.0518 -0.24 0 101 7.1e-07 1 U K.EDVANMDNTPPVVEPPSKPK.Y

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 3115 142 – 161 722.0244 2163.0514 2163.0518 -0.19 0 119 9.3e-09 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3116 142 – 161 722.0245 2163.0517 2163.0518 -0.047 0 111 5.9e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3117 142 – 161 722.0246 2163.0520 2163.0518 0.12 0 117 1.8e-08 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3118 142 – 161 722.0246 2163.0520 2163.0518 0.12 0 119 9.5e-09 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3119 142 – 161 1082.5346 2163.0546 2163.0518 1.33 0 82 5.7e-05 1 U K.EDVANMDNTPPVVEPPSKPK.Y 3109 142 – 161 1082.5303 2163.0460 2163.0882 -19.5 0 56 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M); [-15.9585 at N-term E] 3128 142 – 161 727.3559 2179.0458 2179.0467 -0.42 0 55 0.026 6 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3129 142 – 161 727.3561 2179.0464 2179.0467 -0.16 0 101 7.1e-07 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3130 142 – 161 727.3565 2179.0478 2179.0467 0.52 0 94 3.6e-06 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3131 142 – 161 727.3567 2179.0482 2179.0467 0.71 0 101 6.4e-07 1 U K.EDVANMDNTPPVVEPPSKPK.Y + Oxidation (M) 3132 142 – 161 727.3569 2179.0488 2179.0467 0.99 0 85 5 U K.EDVANMDNTPPVVEPPSKPK.Y + [+15.9949 at A4] 3165 142 – 161 558.2451 2228.9513 2228.9976 -20.8 0 62 1 U K.EDVANMDNTPPVVEPPSKPK.Y + 3 [+21.9819 at E1,D2,D7] 3015 197 – 213 657.6586 1969.9540 1969.9534 0.30 0 131 1 L.SVSIEVPGEEPYHFQPR.L 3016 197 – 213 657.6590 1969.9551 1969.9534 0.86 0 130 1 L.SVSIEVPGEEPYHFQPR.L 2924 198 – 213 628.6482 1882.9229 1882.9214 0.83 0 53 1 S.VSIEVPGEEPYHFQPR.L 2578 200 – 213 566.6140 1696.8203 1696.8209 -0.36 0 109 1 S.IEVPGEEPYHFQPR.L 177 225 – 231 419.7377 837.4608 837.4596 1.45 0 55 0.01 1 K.YQVLSTK.V 178 225 – 231 419.7377 837.4608 837.4596 1.45 0 56 0.0081 1 K.YQVLSTK.V 2476 239 – 252 813.8901 1625.7657 1625.7686 -1.73 0 98 1 K.AEQVTWTTLDYSGR.P 2477 239 – 252 813.8914 1625.7683 1625.7686 -0.18 0 78 1 K.AEQVTWTTLDYSGR.P 2478 239 – 252 542.9301 1625.7686 1625.7686 0.0043 0 65 1 K.AEQVTWTTLDYSGR.P 2480 239 – 252 813.8918 1625.7690 1625.7686 0.30 0 66 1 K.AEQVTWTTLDYSGR.P 2481 239 – 252 813.8919 1625.7692 1625.7686 0.41 0 84 1 K.AEQVTWTTLDYSGR.P 2482 239 – 252 813.8919 1625.7693 1625.7686 0.48 0 103 1 K.AEQVTWTTLDYSGR.P 2483 239 – 252 813.8920 1625.7695 1625.7686 0.57 0 98 1 K.AEQVTWTTLDYSGR.P 2484 239 – 252 542.9305 1625.7698 1625.7686 0.74 0 67 1 K.AEQVTWTTLDYSGR.P 2485 239 – 252 542.9306 1625.7700 1625.7686 0.89 0 70 1 K.AEQVTWTTLDYSGR.P 2487 239 – 252 813.8924 1625.7703 1625.7686 1.05 0 105 1 K.AEQVTWTTLDYSGR.P 2488 239 – 252 813.8925 1625.7704 1625.7686 1.14 0 73 1 K.AEQVTWTTLDYSGR.P 2489 239 – 252 813.8926 1625.7706 1625.7686 1.29 0 61 1 K.AEQVTWTTLDYSGR.P 2490 239 – 252 542.9309 1625.7708 1625.7686 1.35 0 65 1 K.AEQVTWTTLDYSGR.P 2491 239 – 252 813.8929 1625.7712 1625.7686 1.63 0 87 1 K.AEQVTWTTLDYSGR.P 2492 239 – 252 813.8932 1625.7718 1625.7686 2.01 0 69 1 K.AEQVTWTTLDYSGR.P 2495 239 – 252 813.8941 1625.7737 1625.7686 3.17 0 57 1 K.AEQVTWTTLDYSGR.P 2899 239 – 254 926.4630 1850.9115 1850.9163 -2.58 0 91 5.7e-06 1 K.AEQVTWTTLDYSGRPK.A 2900 239 – 254 926.4658 1850.9170 1850.9163 0.42 0 96 1.7e-06 1 K.AEQVTWTTLDYSGRPK.A 2744 260 – 276 606.9797 1817.9174 1817.9159 0.83 0 77 1 K.ISTPAETAPRPSYPSSK.S + [+30.0106 at A5] 2746 260 – 276 606.9802 1817.9188 1817.9159 1.59 0 75 1 K.ISTPAETAPRPSYPSSK.S + [+30.0106 at A5] 2959 260 – 276 633.6344 1897.8814 1897.8822 -0.45 0 63 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2960 260 – 276 633.6348 1897.8825 1897.8822 0.15 0 91 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2961 260 – 276 949.9489 1897.8832 1897.8822 0.53 0 69 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2963 260 – 276 633.6352 1897.8839 1897.8822 0.86 0 87 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2964 260 – 276 949.9493 1897.8841 1897.8822 0.98 0 113 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2965 260 – 276 633.6355 1897.8846 1897.8822 1.22 0 64 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2966 260 – 276 949.9496 1897.8846 1897.8822 1.23 0 72 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2967 260 – 276 949.9496 1897.8847 1897.8822 1.29 0 127 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5] 2968 260 – 276 633.6357 1897.8854 1897.8822 1.66 0 73 1 K.ISTPAETAPRPSYPSSK.S + Phospho (ST); [+30.0106 at A5]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 74 270 – 276 383.1935 764.3724 764.3705 2.51 0 56 1 R.PSYPSSK.S 75 270 – 276 383.1936 764.3726 764.3705 2.82 0 54 1 R.PSYPSSK.S 1705 279 – 289 340.9335 1359.7047 1359.6882 12.2 2 55 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0207 at W3] 1712 279 – 289 340.9335 1359.7048 1359.6882 12.2 2 51 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0207 at W3] 1625 279 – 289 340.9329 1359.7024 1359.7034 -0.76 2 53 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1637 279 – 289 340.9331 1359.7032 1359.7034 -0.15 2 60 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1647 279 – 289 340.9332 1359.7035 1359.7034 0.089 2 60 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1652 279 – 289 454.2418 1359.7037 1359.7034 0.20 2 71 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1653 279 – 289 340.9332 1359.7037 1359.7034 0.21 2 54 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1669 279 – 289 340.9333 1359.7041 1359.7034 0.53 2 54 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1681 279 – 289 340.9334 1359.7043 1359.7034 0.65 2 55 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1685 279 – 289 454.2421 1359.7044 1359.7034 0.71 2 73 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1686 279 – 289 454.2421 1359.7044 1359.7034 0.71 2 72 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1689 279 – 289 454.2421 1359.7044 1359.7034 0.76 2 64 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1690 279 – 289 340.9334 1359.7045 1359.7034 0.80 2 54 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1706 279 – 289 340.9335 1359.7047 1359.7034 0.97 2 52 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1710 279 – 289 454.2422 1359.7048 1359.7034 1.00 2 58 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1732 279 – 289 454.2423 1359.7051 1359.7034 1.24 2 74 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-58.0055 at D4] 1609 279 – 289 340.9326 1359.7013 1359.7245 -17.1 2 53 1 K.KDWDKLEAEVK.K + Carboxymethyl (K); [-57.9843 at W3] 974 280 – 289 616.8099 1231.6053 1231.6084 -2.58 1 70 0.00091 5 K.DWDKLEAEVK.K 975 280 – 289 616.8104 1231.6063 1231.6084 -1.72 1 69 0.00098 5 K.DWDKLEAEVK.K 976 280 – 289 616.8104 1231.6063 1231.6084 -1.72 1 65 0.0026 4 K.DWDKLEAEVK.K 977 280 – 289 616.8104 1231.6063 1231.6084 -1.70 1 67 0.0017 4 K.DWDKLEAEVK.K 978 280 – 289 616.8107 1231.6068 1231.6084 -1.33 1 65 0.0026 5 K.DWDKLEAEVK.K 979 280 – 289 411.5430 1231.6071 1231.6084 -1.13 1 69 0.0011 1 K.DWDKLEAEVK.K 980 280 – 289 616.8108 1231.6071 1231.6084 -1.05 1 65 0.0026 4 K.DWDKLEAEVK.K 981 280 – 289 411.5430 1231.6073 1231.6084 -0.96 1 68 0.0013 4 K.DWDKLEAEVK.K 982 280 – 289 411.5430 1231.6073 1231.6084 -0.94 1 64 0.0036 4 K.DWDKLEAEVK.K 983 280 – 289 616.8110 1231.6074 1231.6084 -0.86 1 69 0.001 5 K.DWDKLEAEVK.K 984 280 – 289 411.5431 1231.6075 1231.6084 -0.79 1 69 0.0011 4 K.DWDKLEAEVK.K 985 280 – 289 411.5431 1231.6075 1231.6084 -0.76 1 68 0.0013 4 K.DWDKLEAEVK.K 986 280 – 289 616.8111 1231.6075 1231.6084 -0.73 1 64 0.0033 4 K.DWDKLEAEVK.K 987 280 – 289 411.5431 1231.6076 1231.6084 -0.72 1 72 0.00047 4 K.DWDKLEAEVK.K 988 280 – 289 616.8111 1231.6077 1231.6084 -0.60 1 72 0.00056 5 K.DWDKLEAEVK.K 990 280 – 289 411.5432 1231.6078 1231.6084 -0.55 1 68 0.0014 4 K.DWDKLEAEVK.K 991 280 – 289 616.8112 1231.6078 1231.6084 -0.50 1 71 0.00058 5 K.DWDKLEAEVK.K 992 280 – 289 616.8112 1231.6078 1231.6084 -0.48 1 71 0.00059 5 K.DWDKLEAEVK.K 993 280 – 289 616.8112 1231.6079 1231.6084 -0.47 1 67 0.0015 4 K.DWDKLEAEVK.K 994 280 – 289 616.8113 1231.6080 1231.6084 -0.37 1 67 0.0016 5 K.DWDKLEAEVK.K 995 280 – 289 616.8113 1231.6080 1231.6084 -0.35 1 72 0.00057 5 K.DWDKLEAEVK.K 996 280 – 289 411.5433 1231.6080 1231.6084 -0.35 1 66 0.0019 4 K.DWDKLEAEVK.K 997 280 – 289 411.5433 1231.6080 1231.6084 -0.33 1 65 0.0024 4 K.DWDKLEAEVK.K 998 280 – 289 616.8113 1231.6080 1231.6084 -0.32 1 67 0.0018 4 K.DWDKLEAEVK.K 999 280 – 289 616.8113 1231.6081 1231.6084 -0.24 1 58 0.013 5 K.DWDKLEAEVK.K 1000 280 – 289 411.5433 1231.6082 1231.6084 -0.20 1 66 0.0023 4 K.DWDKLEAEVK.K 1001 280 – 289 616.8114 1231.6082 1231.6084 -0.19 1 62 0.0054 4 K.DWDKLEAEVK.K 1002 280 – 289 411.5434 1231.6083 1231.6084 -0.16 1 67 0.0017 4 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1003 280 – 289 616.8114 1231.6083 1231.6084 -0.13 1 63 0.0042 5 K.DWDKLEAEVK.K 1004 280 – 289 616.8114 1231.6083 1231.6084 -0.11 1 68 0.0013 4 K.DWDKLEAEVK.K 1005 280 – 289 616.8114 1231.6083 1231.6084 -0.11 1 61 0.0065 4 K.DWDKLEAEVK.K 1006 280 – 289 616.8114 1231.6083 1231.6084 -0.11 1 72 0.00058 5 K.DWDKLEAEVK.K 1007 280 – 289 616.8114 1231.6083 1231.6084 -0.094 1 64 0.0036 4 K.DWDKLEAEVK.K 1008 280 – 289 411.5434 1231.6083 1231.6084 -0.083 1 67 0.0017 4 K.DWDKLEAEVK.K 1009 280 – 289 411.5434 1231.6083 1231.6084 -0.083 1 68 0.0014 4 K.DWDKLEAEVK.K 1010 280 – 289 616.8115 1231.6084 1231.6084 -0.062 1 62 0.0048 5 K.DWDKLEAEVK.K 1011 280 – 289 411.5434 1231.6084 1231.6084 -0.0097 1 64 0.0035 4 K.DWDKLEAEVK.K 1012 280 – 289 411.5434 1231.6084 1231.6084 -0.0097 1 68 0.0013 4 K.DWDKLEAEVK.K 1013 280 – 289 411.5434 1231.6085 1231.6084 0.015 1 66 0.0023 4 K.DWDKLEAEVK.K 1014 280 – 289 616.8115 1231.6085 1231.6084 0.052 1 72 0.00058 5 K.DWDKLEAEVK.K 1015 280 – 289 411.5435 1231.6086 1231.6084 0.088 1 69 0.0011 4 K.DWDKLEAEVK.K 1016 280 – 289 616.8116 1231.6086 1231.6084 0.12 1 69 0.0011 4 K.DWDKLEAEVK.K 1017 280 – 289 616.8116 1231.6086 1231.6084 0.12 1 63 0.0037 4 K.DWDKLEAEVK.K 1018 280 – 289 616.8116 1231.6086 1231.6084 0.15 1 65 0.0026 4 K.DWDKLEAEVK.K 1019 280 – 289 616.8116 1231.6086 1231.6084 0.15 1 61 0.0067 5 K.DWDKLEAEVK.K 1020 280 – 289 616.8116 1231.6086 1231.6084 0.15 1 68 0.0013 4 K.DWDKLEAEVK.K 1021 280 – 289 411.5435 1231.6086 1231.6084 0.16 1 67 0.0017 4 K.DWDKLEAEVK.K 1022 280 – 289 616.8116 1231.6086 1231.6084 0.17 1 65 0.0028 5 K.DWDKLEAEVK.K 1023 280 – 289 411.5435 1231.6087 1231.6084 0.21 1 68 0.0014 4 K.DWDKLEAEVK.K 1024 280 – 289 411.5435 1231.6087 1231.6084 0.21 1 66 0.0019 4 K.DWDKLEAEVK.K 1025 280 – 289 411.5435 1231.6087 1231.6084 0.21 1 68 0.0012 4 K.DWDKLEAEVK.K 1026 280 – 289 616.8116 1231.6087 1231.6084 0.25 1 59 0.011 4 K.DWDKLEAEVK.K 1027 280 – 289 411.5435 1231.6088 1231.6084 0.26 1 67 0.0018 4 K.DWDKLEAEVK.K 1028 280 – 289 616.8117 1231.6088 1231.6084 0.28 1 67 0.0016 5 K.DWDKLEAEVK.K 1029 280 – 289 411.5435 1231.6088 1231.6084 0.28 1 76 0.00019 4 K.DWDKLEAEVK.K 1030 280 – 289 616.8117 1231.6088 1231.6084 0.30 1 66 0.0018 5 K.DWDKLEAEVK.K 1031 280 – 289 616.8117 1231.6088 1231.6084 0.30 1 65 0.0026 4 K.DWDKLEAEVK.K 1032 280 – 289 616.8117 1231.6088 1231.6084 0.31 1 65 0.0027 4 K.DWDKLEAEVK.K 1033 280 – 289 616.8117 1231.6088 1231.6084 0.33 1 65 0.0026 4 K.DWDKLEAEVK.K 1034 280 – 289 616.8117 1231.6089 1231.6084 0.34 1 61 0.007 5 K.DWDKLEAEVK.K 1035 280 – 289 616.8117 1231.6089 1231.6084 0.38 1 69 0.00097 5 K.DWDKLEAEVK.K 1036 280 – 289 616.8117 1231.6089 1231.6084 0.38 1 62 0.005 5 K.DWDKLEAEVK.K 1037 280 – 289 411.5436 1231.6089 1231.6084 0.38 1 66 0.0019 4 K.DWDKLEAEVK.K 1038 280 – 289 411.5436 1231.6089 1231.6084 0.38 1 76 0.00019 4 K.DWDKLEAEVK.K 1039 280 – 289 411.5436 1231.6089 1231.6084 0.38 1 64 0.0033 4 K.DWDKLEAEVK.K 1040 280 – 289 616.8117 1231.6089 1231.6084 0.39 1 63 0.004 5 K.DWDKLEAEVK.K 1041 280 – 289 616.8117 1231.6089 1231.6084 0.41 1 69 0.001 5 K.DWDKLEAEVK.K 1042 280 – 289 616.8117 1231.6089 1231.6084 0.41 1 69 0.001 5 K.DWDKLEAEVK.K 1043 280 – 289 616.8117 1231.6089 1231.6084 0.41 1 59 0.0097 5 K.DWDKLEAEVK.K 1044 280 – 289 411.5436 1231.6090 1231.6084 0.43 1 52 0.048 1 K.DWDKLEAEVK.K 1045 280 – 289 411.5436 1231.6090 1231.6084 0.45 1 64 0.0035 4 K.DWDKLEAEVK.K 1046 280 – 289 411.5436 1231.6090 1231.6084 0.45 1 68 0.0012 4 K.DWDKLEAEVK.K 1047 280 – 289 616.8118 1231.6090 1231.6084 0.46 1 65 0.0027 4 K.DWDKLEAEVK.K 1048 280 – 289 616.8118 1231.6090 1231.6084 0.47 1 65 0.0026 4 K.DWDKLEAEVK.K 1049 280 – 289 411.5436 1231.6091 1231.6084 0.50 1 68 0.0014 4 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1050 280 – 289 616.8118 1231.6091 1231.6084 0.51 1 67 0.0017 4 K.DWDKLEAEVK.K 1051 280 – 289 616.8118 1231.6091 1231.6084 0.52 1 65 0.0026 3 K.DWDKLEAEVK.K 1052 280 – 289 616.8118 1231.6091 1231.6084 0.54 1 62 0.0054 4 K.DWDKLEAEVK.K 1053 280 – 289 411.5437 1231.6091 1231.6084 0.55 1 67 0.0018 4 K.DWDKLEAEVK.K 1054 280 – 289 411.5437 1231.6091 1231.6084 0.55 1 63 0.0042 4 K.DWDKLEAEVK.K 1055 280 – 289 616.8119 1231.6091 1231.6084 0.57 1 66 0.002 4 K.DWDKLEAEVK.K 1056 280 – 289 616.8119 1231.6092 1231.6084 0.59 1 59 0.011 4 K.DWDKLEAEVK.K 1057 280 – 289 411.5437 1231.6092 1231.6084 0.60 1 69 0.00099 4 K.DWDKLEAEVK.K 1058 280 – 289 616.8119 1231.6092 1231.6084 0.60 1 67 0.0017 5 K.DWDKLEAEVK.K 1059 280 – 289 616.8119 1231.6092 1231.6084 0.60 1 58 0.014 5 K.DWDKLEAEVK.K 1060 280 – 289 616.8119 1231.6092 1231.6084 0.62 1 64 0.0031 5 K.DWDKLEAEVK.K 1061 280 – 289 411.5437 1231.6092 1231.6084 0.62 1 53 0.043 1 K.DWDKLEAEVK.K 1062 280 – 289 411.5437 1231.6092 1231.6084 0.62 1 68 0.0012 4 K.DWDKLEAEVK.K 1063 280 – 289 616.8119 1231.6092 1231.6084 0.65 1 53 0.039 5 K.DWDKLEAEVK.K 1064 280 – 289 616.8119 1231.6092 1231.6084 0.65 1 72 0.00058 5 K.DWDKLEAEVK.K 1065 280 – 289 411.5437 1231.6093 1231.6084 0.67 1 66 0.0021 4 K.DWDKLEAEVK.K 1066 280 – 289 616.8119 1231.6093 1231.6084 0.69 1 71 0.00059 5 K.DWDKLEAEVK.K 1067 280 – 289 616.8119 1231.6093 1231.6084 0.70 1 68 0.0014 4 K.DWDKLEAEVK.K 1068 280 – 289 616.8119 1231.6093 1231.6084 0.72 1 65 0.0026 3 K.DWDKLEAEVK.K 1069 280 – 289 411.5437 1231.6094 1231.6084 0.75 1 70 0.00085 4 K.DWDKLEAEVK.K 1070 280 – 289 411.5437 1231.6094 1231.6084 0.75 1 69 0.00095 4 K.DWDKLEAEVK.K 1071 280 – 289 411.5437 1231.6094 1231.6084 0.75 1 68 0.0012 4 K.DWDKLEAEVK.K 1072 280 – 289 411.5437 1231.6094 1231.6084 0.75 1 66 0.0021 4 K.DWDKLEAEVK.K 1073 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 72 0.00057 5 K.DWDKLEAEVK.K 1074 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 63 0.0039 4 K.DWDKLEAEVK.K 1075 280 – 289 616.8120 1231.6094 1231.6084 0.75 1 67 0.0015 5 K.DWDKLEAEVK.K 1076 280 – 289 616.8120 1231.6094 1231.6084 0.77 1 67 0.0016 5 K.DWDKLEAEVK.K 1077 280 – 289 616.8120 1231.6094 1231.6084 0.77 1 65 0.0026 4 K.DWDKLEAEVK.K 1078 280 – 289 616.8120 1231.6094 1231.6084 0.77 1 67 0.0018 4 K.DWDKLEAEVK.K 1079 280 – 289 411.5437 1231.6094 1231.6084 0.77 1 71 0.00069 4 K.DWDKLEAEVK.K 1080 280 – 289 411.5437 1231.6094 1231.6084 0.79 1 70 0.0009 4 K.DWDKLEAEVK.K 1081 280 – 289 411.5437 1231.6094 1231.6084 0.79 1 66 0.0019 4 K.DWDKLEAEVK.K 1082 280 – 289 411.5437 1231.6094 1231.6084 0.79 1 70 0.00089 4 K.DWDKLEAEVK.K 1083 280 – 289 616.8120 1231.6094 1231.6084 0.80 1 68 0.0013 4 K.DWDKLEAEVK.K 1084 280 – 289 616.8120 1231.6094 1231.6084 0.82 1 72 0.00058 5 K.DWDKLEAEVK.K 1085 280 – 289 411.5438 1231.6095 1231.6084 0.82 1 71 0.00068 4 K.DWDKLEAEVK.K 1086 280 – 289 616.8120 1231.6095 1231.6084 0.83 1 62 0.0055 4 K.DWDKLEAEVK.K 1087 280 – 289 411.5438 1231.6095 1231.6084 0.84 1 68 0.0012 4 K.DWDKLEAEVK.K 1088 280 – 289 616.8120 1231.6095 1231.6084 0.86 1 61 0.0067 5 K.DWDKLEAEVK.K 1089 280 – 289 411.5438 1231.6095 1231.6084 0.87 1 70 0.00092 4 K.DWDKLEAEVK.K 1090 280 – 289 616.8120 1231.6095 1231.6084 0.88 1 80 8.9e-05 5 K.DWDKLEAEVK.K 1091 280 – 289 411.5438 1231.6095 1231.6084 0.89 1 65 0.0026 4 K.DWDKLEAEVK.K 1092 280 – 289 616.8120 1231.6095 1231.6084 0.90 1 70 0.0008 4 K.DWDKLEAEVK.K 1093 280 – 289 616.8121 1231.6096 1231.6084 0.91 1 59 0.01 6 K.DWDKLEAEVK.K 1094 280 – 289 616.8121 1231.6096 1231.6084 0.91 1 54 0.034 5 K.DWDKLEAEVK.K 1095 280 – 289 411.5438 1231.6096 1231.6084 0.92 1 69 0.001 1 K.DWDKLEAEVK.K 1096 280 – 289 411.5438 1231.6096 1231.6084 0.92 1 70 0.00083 4 K.DWDKLEAEVK.K

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1193 280 – 289 616.8126 1231.6107 1231.6084 1.82 1 68 0.0012 5 K.DWDKLEAEVK.K 1194 280 – 289 616.8126 1231.6107 1231.6084 1.82 1 62 0.0049 4 K.DWDKLEAEVK.K 1196 280 – 289 411.5442 1231.6107 1231.6084 1.87 1 66 0.0019 4 K.DWDKLEAEVK.K 1197 280 – 289 616.8126 1231.6107 1231.6084 1.87 1 65 0.0025 4 K.DWDKLEAEVK.K 1198 280 – 289 411.5443 1231.6110 1231.6084 2.04 1 69 0.0011 4 K.DWDKLEAEVK.K 1199 280 – 289 411.5443 1231.6112 1231.6084 2.23 1 67 0.0017 4 K.DWDKLEAEVK.K 1200 280 – 289 411.5443 1231.6112 1231.6084 2.23 1 63 0.004 4 K.DWDKLEAEVK.K 1202 280 – 289 412.8744 1235.6015 1235.6034 -1.51 1 55 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1203 280 – 289 412.8745 1235.6016 1235.6034 -1.39 1 55 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1204 280 – 289 618.8082 1235.6018 1235.6034 -1.23 1 52 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1205 280 – 289 618.8083 1235.6019 1235.6034 -1.15 1 51 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1206 280 – 289 412.8748 1235.6024 1235.6034 -0.76 1 51 1 K.DWDKLEAEVK.K + [+3.9949 at W2] 1234 280 – 289 624.8076 1247.6006 1247.6034 -2.22 1 67 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1235 280 – 289 624.8076 1247.6006 1247.6034 -2.19 1 81 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1236 280 – 289 624.8079 1247.6013 1247.6034 -1.63 1 55 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1237 280 – 289 416.8746 1247.6019 1247.6034 -1.19 1 68 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1238 280 – 289 416.8746 1247.6019 1247.6034 -1.14 1 55 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1240 280 – 289 624.8084 1247.6022 1247.6034 -0.89 1 83 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1241 280 – 289 416.8747 1247.6023 1247.6034 -0.87 1 68 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1242 280 – 289 624.8086 1247.6027 1247.6034 -0.51 1 63 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1243 280 – 289 624.8089 1247.6033 1247.6034 -0.028 1 81 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1245 280 – 289 624.8091 1247.6037 1247.6034 0.26 1 72 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1246 280 – 289 624.8093 1247.6040 1247.6034 0.50 1 86 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1247 280 – 289 624.8093 1247.6040 1247.6034 0.53 1 67 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1248 280 – 289 416.8754 1247.6043 1247.6034 0.76 1 81 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1250 280 – 289 624.8096 1247.6047 1247.6034 1.08 1 88 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1251 280 – 289 416.8755 1247.6047 1247.6034 1.10 1 66 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1253 280 – 289 416.8756 1247.6050 1247.6034 1.31 1 79 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1254 280 – 289 416.8757 1247.6052 1247.6034 1.43 1 55 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1255 280 – 289 416.8758 1247.6056 1247.6034 1.77 1 76 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1256 280 – 289 624.8103 1247.6060 1247.6034 2.09 1 70 1 K.DWDKLEAEVK.K + [+15.9949 at W2] 1269 280 – 289 627.7975 1253.5805 1253.5904 -7.87 1 57 1 K.DWDKLEAEVK.K + [+21.9819 at E6] 1271 280 – 289 627.8004 1253.5863 1253.5904 -3.27 1 67 1 K.DWDKLEAEVK.K + [+21.9819 at E8] 1280 280 – 289 629.8203 1257.6261 1257.6241 1.64 1 53 1 K.DWDKLEAEVK.K + [+26.0156 at N-term] 1281 280 – 289 420.2162 1257.6267 1257.6241 2.04 1 56 1 K.DWDKLEAEVK.K + [+26.0156 at N-term] 1285 280 – 289 630.8068 1259.5991 1259.6034 -3.41 1 56 1 K.DWDKLEAEVK.K + [+27.9949 at N-term] 1287 280 – 289 630.8075 1259.6004 1259.6034 -2.36 1 53 1 K.DWDKLEAEVK.K + [+27.9949 at N-term] 1293 280 – 289 632.8053 1263.5960 1263.5983 -1.79 1 54 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1294 280 – 289 422.2060 1263.5963 1263.5983 -1.59 1 54 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1296 280 – 289 632.8060 1263.5974 1263.5983 -0.66 1 51 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1297 280 – 289 422.2067 1263.5982 1263.5983 -0.044 1 58 1 K.DWDKLEAEVK.K + [+31.9898 at W2] 1291 280 – 289 632.7958 1263.5770 1263.5983 -16.9 1 54 1 K.DWDKLEAEVK.K + 2 [+15.9949 at D1,W2] 1361 280 – 289 430.5500 1288.6283 1288.6299 -1.28 1 53 1 K.DWDKLEAEVK.K + [+57.0215 at K4] 1362 280 – 289 645.3216 1288.6286 1288.6299 -1.01 1 61 1 K.DWDKLEAEVK.K + [+57.0215 at K4] 1667 280 – 289 454.2420 1359.7041 1359.7034 0.50 1 61 1 K.DWDKLEAEVK.K + [+128.0950 at C-term K] 2546 291 – 305 562.2807 1683.8202 1683.8315 -6.73 2 105 1 K.EEKEEKLEGDAALNK.F + [-18.0106 at N-term] 2590 291 – 305 568.2880 1701.8422 1701.8421 0.086 2 102 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2591 291 – 305 568.2881 1701.8425 1701.8421 0.24 2 105 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2592 291 – 305 568.2881 1701.8425 1701.8421 0.26 2 112 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2593 291 – 305 568.2881 1701.8426 1701.8421 0.33 2 124 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2594 291 – 305 568.2882 1701.8427 1701.8421 0.39 2 82 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2598 291 – 305 568.2883 1701.8432 1701.8421 0.67 2 74 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2600 291 – 305 568.2884 1701.8434 1701.8421 0.81 2 128 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2603 291 – 305 851.9293 1701.8440 1701.8421 1.16 2 101 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2604 291 – 305 568.2886 1701.8440 1701.8421 1.16 2 119 9 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E5] 2609 291 – 305 426.4687 1701.8455 1701.8421 2.02 2 53 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [-58.0055 at E4] 2688 291 – 305 590.2553 1767.7441 1767.7879 -24.8 2 95 5 K.EEKEEKLEGDAALNK.F + 3 [+21.9819 at E1,E2,E4] 2690 291 – 305 590.2554 1767.7443 1767.7879 -24.6 2 80 5 K.EEKEEKLEGDAALNK.F + 3 [+21.9819 at E2,E4,E5] 2691 291 – 305 590.2557 1767.7452 1767.7879 -24.1 2 56 5 K.EEKEEKLEGDAALNK.F + 3 [+21.9819 at E2,E4,E5] 2736 291 – 305 604.6399 1810.8980 1810.8948 1.78 2 58 1 K.EEKEEKLEGDAALNK.F + [+109.0528 at K6] 2761 291 – 305 458.4911 1829.9352 1829.8806 29.8 2 51 1 K.EEKEEKLEGDAALNK.F + 2 Carboxymethyl (K); 2 [+6.0138 at E4,E5] 2751 291 – 305 610.9849 1829.9328 1829.9370 -2.30 2 104 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2753 291 – 305 915.9742 1829.9338 1829.9370 -1.72 2 131 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2754 291 – 305 610.9854 1829.9343 1829.9370 -1.47 2 120 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2755 291 – 305 610.9854 1829.9343 1829.9370 -1.45 2 119 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2758 291 – 305 610.9856 1829.9349 1829.9370 -1.14 2 124 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2760 291 – 305 610.9856 1829.9351 1829.9370 -1.04 2 130 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2767 291 – 305 458.4913 1829.9360 1829.9370 -0.56 2 55 1 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2769 291 – 305 458.4914 1829.9365 1829.9370 -0.30 2 54 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2772 291 – 305 610.9861 1829.9366 1829.9370 -0.24 2 121 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2775 291 – 305 610.9864 1829.9373 1829.9370 0.14 2 131 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2777 291 – 305 610.9864 1829.9374 1829.9370 0.24 2 137 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2779 291 – 305 610.9864 1829.9375 1829.9370 0.25 2 139 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2783 291 – 305 610.9865 1829.9376 1829.9370 0.34 2 127 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2784 291 – 305 610.9865 1829.9376 1829.9370 0.35 2 120 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2786 291 – 305 610.9865 1829.9377 1829.9370 0.37 2 139 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2788 291 – 305 610.9865 1829.9377 1829.9370 0.40 2 130 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2790 291 – 305 458.4917 1829.9378 1829.9370 0.43 2 93 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2792 291 – 305 458.4917 1829.9378 1829.9370 0.45 2 71 1 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2795 291 – 305 610.9866 1829.9379 1829.9370 0.52 2 102 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2797 291 – 305 458.4918 1829.9380 1829.9370 0.56 2 59 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2799 291 – 305 458.4918 1829.9381 1829.9370 0.58 2 81 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2803 291 – 305 610.9867 1829.9383 1829.9370 0.71 2 123 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2808 291 – 305 458.4919 1829.9384 1829.9370 0.75 2 87 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2809 291 – 305 610.9867 1829.9384 1829.9370 0.76 2 119 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2812 291 – 305 366.9950 1829.9386 1829.9370 0.86 2 58 4 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2819 291 – 305 610.9869 1829.9388 1829.9370 0.96 2 122 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2822 291 – 305 610.9869 1829.9389 1829.9370 1.04 2 139 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2823 291 – 305 610.9869 1829.9389 1829.9370 1.04 2 139 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2826 291 – 305 458.4920 1829.9390 1829.9370 1.08 2 97 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2827 291 – 305 458.4920 1829.9390 1829.9370 1.08 2 84 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2828 291 – 305 458.4920 1829.9390 1829.9370 1.08 2 87 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2831 291 – 305 458.4920 1829.9390 1829.9370 1.10 2 60 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2833 291 – 305 458.4920 1829.9391 1829.9370 1.13 2 91 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term]

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 2838 291 – 305 458.4921 1829.9391 1829.9370 1.17 2 81 10 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2840 291 – 305 610.9870 1829.9391 1829.9370 1.17 2 85 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2841 291 – 305 610.9870 1829.9392 1829.9370 1.19 2 132 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2842 291 – 305 915.9769 1829.9392 1829.9370 1.20 2 132 4 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2843 291 – 305 610.9870 1829.9392 1829.9370 1.21 2 80 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2848 291 – 305 610.9871 1829.9395 1829.9370 1.37 2 114 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2849 291 – 305 366.9952 1829.9395 1829.9370 1.38 2 52 4 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2852 291 – 305 458.4922 1829.9396 1829.9370 1.41 2 88 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2855 291 – 305 610.9872 1829.9398 1829.9370 1.52 2 79 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2856 291 – 305 915.9772 1829.9399 1829.9370 1.59 2 129 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2858 291 – 305 610.9873 1829.9400 1829.9370 1.65 2 113 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2861 291 – 305 458.4923 1829.9402 1829.9370 1.74 2 91 7 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2864 291 – 305 610.9874 1829.9403 1829.9370 1.78 2 70 6 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2866 291 – 305 458.4923 1829.9403 1829.9370 1.78 2 89 9 K.EEKEEKLEGDAALNK.F + [+128.0950 at K6] 2870 291 – 305 915.9775 1829.9404 1829.9370 1.85 2 132 4 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2875 291 – 305 458.4925 1829.9408 1829.9370 2.07 2 84 8 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2878 291 – 305 915.9781 1829.9416 1829.9370 2.54 2 90 5 K.EEKEEKLEGDAALNK.F + [+128.0950 at N-term] 2901 291 – 305 618.3107 1851.9103 1851.9220 -6.30 2 62 1 K.EEKEEKLEGDAALNK.F + 2 Carboxymethyl (K); 2 [+17.0345 at E1,E5] 2933 291 – 305 948.9244 1895.8342 1895.8764 -22.3 2 55 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2936 291 – 305 632.9526 1895.8361 1895.8764 -21.3 2 56 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2940 291 – 305 632.9529 1895.8368 1895.8764 -20.9 2 54 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2941 291 – 305 474.9665 1895.8369 1895.8764 -20.8 2 53 3 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at K6] 2943 291 – 305 632.9532 1895.8379 1895.8764 -20.4 2 52 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2944 291 – 305 632.9533 1895.8382 1895.8764 -20.2 2 60 2 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2948 291 – 305 632.9534 1895.8384 1895.8764 -20.1 2 51 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 2949 291 – 305 632.9535 1895.8387 1895.8764 -19.9 2 68 1 K.EEKEEKLEGDAALNK.F + Carboxymethyl (K); [+136.0289 at N-term] 1408 294 – 305 433.5581 1297.6526 1297.6514 0.93 1 72 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1409 294 – 305 433.5582 1297.6528 1297.6514 1.14 1 72 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1410 294 – 305 433.5584 1297.6533 1297.6514 1.53 1 54 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1411 294 – 305 433.5586 1297.6541 1297.6514 2.11 1 68 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1412 294 – 305 433.5587 1297.6542 1297.6514 2.23 1 72 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1413 294 – 305 433.5589 1297.6548 1297.6514 2.69 1 68 1 K.EEKLEGDAALNK.F + [-18.0106 at N-term] 1452 294 – 305 658.8351 1315.6556 1315.6619 -4.79 1 59 0.0089 1 K.EEKLEGDAALNK.F 1456 294 – 305 439.5605 1315.6596 1315.6619 -1.75 1 92 4.6e-06 1 K.EEKLEGDAALNK.F 1459 294 – 305 439.5609 1315.6610 1315.6619 -0.68 1 87 1.3e-05 1 K.EEKLEGDAALNK.F 1462 294 – 305 439.5610 1315.6612 1315.6619 -0.56 1 66 0.0017 1 K.EEKLEGDAALNK.F 1466 294 – 305 439.5611 1315.6616 1315.6619 -0.22 1 92 4.6e-06 1 K.EEKLEGDAALNK.F 1467 294 – 305 439.5612 1315.6618 1315.6619 -0.060 1 87 1.4e-05 1 K.EEKLEGDAALNK.F 1470 294 – 305 439.5614 1315.6623 1315.6619 0.26 1 57 0.013 1 K.EEKLEGDAALNK.F 1471 294 – 305 439.5614 1315.6625 1315.6619 0.42 1 54 0.026 11 K.EEKLEGDAALNK.F 1472 294 – 305 658.8385 1315.6625 1315.6619 0.45 1 63 0.0036 1 K.EEKLEGDAALNK.F 1473 294 – 305 658.8385 1315.6625 1315.6619 0.45 1 91 4.9e-06 1 K.EEKLEGDAALNK.F 1476 294 – 305 439.5615 1315.6626 1315.6619 0.53 1 52 0.041 2 K.EEKLEGDAALNK.F 1477 294 – 305 439.5615 1315.6626 1315.6619 0.53 1 94 2.6e-06 1 K.EEKLEGDAALNK.F 1483 294 – 305 658.8387 1315.6629 1315.6619 0.74 1 89 8.3e-06 1 K.EEKLEGDAALNK.F 1484 294 – 305 439.5616 1315.6629 1315.6619 0.76 1 90 6.2e-06 1 K.EEKLEGDAALNK.F 1485 294 – 305 439.5616 1315.6629 1315.6619 0.76 1 85 2.4e-05 1 K.EEKLEGDAALNK.F

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1488 294 – 305 658.8388 1315.6630 1315.6619 0.86 1 86 1.7e-05 1 K.EEKLEGDAALNK.F 1489 294 – 305 658.8388 1315.6631 1315.6619 0.88 1 77 0.00015 1 K.EEKLEGDAALNK.F 1491 294 – 305 439.5617 1315.6632 1315.6619 0.99 1 96 1.5e-06 1 K.EEKLEGDAALNK.F 1492 294 – 305 658.8389 1315.6633 1315.6619 1.05 1 75 0.0002 1 K.EEKLEGDAALNK.F 1494 294 – 305 439.5618 1315.6635 1315.6619 1.24 1 85 2.3e-05 1 K.EEKLEGDAALNK.F 1498 294 – 305 658.8392 1315.6637 1315.6619 1.40 1 79 8e-05 1 K.EEKLEGDAALNK.F 1501 294 – 305 439.5619 1315.6639 1315.6619 1.49 1 85 2.4e-05 1 K.EEKLEGDAALNK.F 1503 294 – 305 658.8393 1315.6641 1315.6619 1.64 1 84 2.7e-05 1 K.EEKLEGDAALNK.F 1505 294 – 305 658.8393 1315.6641 1315.6619 1.67 1 86 1.7e-05 1 K.EEKLEGDAALNK.F 1508 294 – 305 439.5621 1315.6646 1315.6619 2.06 1 87 1.3e-05 1 K.EEKLEGDAALNK.F 1510 294 – 305 658.8397 1315.6648 1315.6619 2.20 1 60 0.0069 1 K.EEKLEGDAALNK.F 1512 294 – 305 658.8399 1315.6652 1315.6619 2.51 1 63 0.0036 1 K.EEKLEGDAALNK.F 1554 294 – 305 669.8269 1337.6392 1337.6439 -3.49 1 60 1 K.EEKLEGDAALNK.F + [+21.9819 at D7] 403 297 – 305 465.7489 929.4833 929.4818 1.62 0 60 0.006 1 K.LEGDAALNK.F 404 297 – 305 465.7492 929.4838 929.4818 2.14 0 74 0.0003 1 K.LEGDAALNK.F 1792 309 – 319 685.7976 1369.5806 1369.5820 -1.01 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1793 309 – 319 685.7976 1369.5806 1369.5820 -0.98 1 93 4.2e-06 1 R.DIYKDADEDMR.R 1794 309 – 319 685.7977 1369.5808 1369.5820 -0.82 1 103 4.2e-07 1 R.DIYKDADEDMR.R 1796 309 – 319 457.5343 1369.5810 1369.5820 -0.68 1 81 7.6e-05 1 R.DIYKDADEDMR.R 1797 309 – 319 685.7978 1369.5811 1369.5820 -0.66 1 98 1.5e-06 1 R.DIYKDADEDMR.R 1798 309 – 319 685.7979 1369.5811 1369.5820 -0.60 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1799 309 – 319 457.5344 1369.5813 1369.5820 -0.46 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1800 309 – 319 457.5344 1369.5815 1369.5820 -0.35 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1801 309 – 319 457.5345 1369.5816 1369.5820 -0.24 1 89 1e-05 1 R.DIYKDADEDMR.R 1802 309 – 319 457.5347 1369.5822 1369.5820 0.13 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1803 309 – 319 685.7984 1369.5822 1369.5820 0.14 1 98 1.5e-06 1 R.DIYKDADEDMR.R 1804 309 – 319 457.5347 1369.5823 1369.5820 0.22 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1805 309 – 319 685.7984 1369.5823 1369.5820 0.26 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1806 309 – 319 457.5347 1369.5824 1369.5820 0.29 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1807 309 – 319 685.7985 1369.5824 1369.5820 0.35 1 97 1.6e-06 1 R.DIYKDADEDMR.R 1809 309 – 319 457.5348 1369.5825 1369.5820 0.37 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1810 309 – 319 685.7986 1369.5826 1369.5820 0.45 1 103 4.2e-07 1 R.DIYKDADEDMR.R 1811 309 – 319 457.5348 1369.5826 1369.5820 0.48 1 87 1.7e-05 1 R.DIYKDADEDMR.R 1812 309 – 319 457.5349 1369.5828 1369.5820 0.61 1 88 1.3e-05 1 R.DIYKDADEDMR.R 1813 309 – 319 685.7988 1369.5831 1369.5820 0.83 1 90 9.8e-06 1 R.DIYKDADEDMR.R 1814 309 – 319 457.5350 1369.5832 1369.5820 0.88 1 87 1.8e-05 1 R.DIYKDADEDMR.R 1816 309 – 319 457.5353 1369.5840 1369.5820 1.45 1 83 4.7e-05 1 R.DIYKDADEDMR.R 1879 309 – 319 692.8059 1383.5972 1383.5976 -0.29 1 73 1 R.DIYKDADEDMR.R + [+14.0157 at D9] 1880 309 – 319 692.8062 1383.5978 1383.5976 0.16 1 68 1 R.DIYKDADEDMR.R + [+14.0157 at D9] 1881 309 – 319 462.2069 1383.5990 1383.5976 0.99 1 80 1 R.DIYKDADEDMR.R + [+14.0157 at E8] 1882 309 – 319 462.2070 1383.5993 1383.5976 1.20 1 80 1 R.DIYKDADEDMR.R + [+14.0157 at E8] 1885 309 – 319 462.8652 1385.5737 1385.5769 -2.27 1 54 1 R.DIYKDADEDMR.R + [+15.9949 at Y3] 1886 309 – 319 693.7957 1385.5768 1385.5769 -0.044 1 86 1.7e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1887 309 – 319 693.7958 1385.5770 1385.5769 0.071 1 61 0.0062 1 R.DIYKDADEDMR.R + Oxidation (M) 1888 309 – 319 462.8664 1385.5773 1385.5769 0.33 1 65 0.0021 1 R.DIYKDADEDMR.R + Oxidation (M) 1889 309 – 319 462.8665 1385.5776 1385.5769 0.52 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1890 309 – 319 693.7961 1385.5777 1385.5769 0.61 1 82 4.9e-05 1 R.DIYKDADEDMR.R + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1891 309 – 319 462.8667 1385.5782 1385.5769 0.93 1 86 1.7e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1892 309 – 319 693.7966 1385.5787 1385.5769 1.30 1 84 2.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1893 309 – 319 693.7966 1385.5787 1385.5769 1.34 1 79 8.5e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1894 309 – 319 693.7967 1385.5788 1385.5769 1.40 1 79 8.7e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1895 309 – 319 693.7968 1385.5790 1385.5769 1.50 1 68 0.001 1 R.DIYKDADEDMR.R + Oxidation (M) 1896 309 – 319 462.8669 1385.5790 1385.5769 1.54 1 82 4.2e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1897 309 – 319 693.7968 1385.5790 1385.5769 1.54 1 90 7.9e-06 1 R.DIYKDADEDMR.R + Oxidation (M) 1898 309 – 319 462.8670 1385.5791 1385.5769 1.56 1 78 0.00011 1 R.DIYKDADEDMR.R + Oxidation (M) 1900 309 – 319 462.8671 1385.5795 1385.5769 1.86 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1901 309 – 319 693.7971 1385.5796 1385.5769 1.96 1 83 3.4e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1902 309 – 319 462.8672 1385.5797 1385.5769 1.99 1 57 1 R.DIYKDADEDMR.R + [+15.9949 at Y3] 1903 309 – 319 462.8672 1385.5797 1385.5769 2.02 1 83 4.1e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1904 309 – 319 693.7971 1385.5797 1385.5769 2.03 1 84 3.2e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1905 309 – 319 462.8672 1385.5798 1385.5769 2.08 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1906 309 – 319 462.8673 1385.5802 1385.5769 2.41 1 86 1.8e-05 1 R.DIYKDADEDMR.R + Oxidation (M) 1975 309 – 319 471.2085 1410.6036 1410.6085 -3.52 1 61 1 R.DIYKDADEDMR.R + [+41.0265 at K4] 1976 309 – 319 706.3110 1410.6075 1410.6085 -0.75 1 78 1 R.DIYKDADEDMR.R + [+41.0265 at K4] 1977 309 – 319 706.3120 1410.6095 1410.6085 0.67 1 61 1 R.DIYKDADEDMR.R + [+41.0265 at K4] 2033 309 – 319 718.7473 1435.4800 1435.4963 -11.4 1 61 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2034 309 – 319 718.7474 1435.4803 1435.4963 -11.2 1 70 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2035 309 – 319 718.7477 1435.4808 1435.4963 -10.8 1 58 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2036 309 – 319 718.7477 1435.4808 1435.4963 -10.7 1 74 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2040 309 – 319 718.7479 1435.4812 1435.4963 -10.5 1 66 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2045 309 – 319 718.7486 1435.4826 1435.4963 -9.49 1 74 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2047 309 – 319 718.7489 1435.4832 1435.4963 -9.10 1 52 1 R.DIYKDADEDMR.R + Phospho (Y); [-14.0520 at K4] 2056 309 – 319 719.7506 1437.4867 1437.5119 -17.6 1 57 4 R.DIYKDADEDMR.R + Phospho (Y); [-12.0364 at I2] 2220 309 – 319 509.5675 1525.6807 1525.6606 13.1 1 52 1 R.DIYKDADEDMR.R + [+156.0786 at K4] 1808 309 – 320 685.7985 1369.5825 1369.5820 0.36 2 103 1 R.DIYKDADEDMRR.A + [-156.1011 at C-term] 1815 309 – 320 685.7990 1369.5834 1369.5820 1.02 2 93 1 R.DIYKDADEDMRR.A + [-156.1011 at C-term] 2281 309 – 320 382.6787 1526.6857 1526.6801 3.68 2 56 1 R.DIYKDADEDMRR.A + [+0.9970 at I2] 2283 309 – 320 764.3520 1526.6895 1526.6801 6.18 2 54 1 R.DIYKDADEDMRR.A + [+0.9970 at I2] 2299 309 – 320 514.8992 1541.6757 1541.6780 -1.48 2 54 1 R.DIYKDADEDMRR.A + [+15.9949 at Y3] 2323 309 – 320 514.9009 1541.6808 1541.6780 1.79 2 54 10 R.DIYKDADEDMRR.A + [+15.9949 at C-term R] 2331 309 – 320 514.9011 1541.6813 1541.6780 2.16 2 80 10 R.DIYKDADEDMRR.A + [+15.9949 at R11] 2332 309 – 320 386.4276 1541.6813 1541.6780 2.17 2 59 10 R.DIYKDADEDMRR.A + [+15.9949 at R11] 2337 309 – 320 386.4277 1541.6818 1541.6780 2.48 2 60 10 R.DIYKDADEDMRR.A + [+15.9949 at R11] 2384 309 – 320 522.2172 1563.6298 1563.6390 -5.84 2 65 1 R.DIYKDADEDMRR.A + [+37.9559 at E8] 2410 309 – 320 396.6833 1582.7042 1582.7045 -0.23 2 61 1 R.DIYKDADEDMRR.A + [+57.0215 at K4] 2413 309 – 320 528.5757 1582.7054 1582.7045 0.55 2 60 1 R.DIYKDADEDMRR.A + Oxidation (M); [+41.0266 at N-term] 2415 309 – 320 528.5760 1582.7061 1582.7045 0.95 2 66 1 R.DIYKDADEDMRR.A + Oxidation (M); [+41.0266 at N-term] 2418 309 – 320 396.6841 1582.7073 1582.7045 1.74 2 53 1 R.DIYKDADEDMRR.A + Oxidation (M); [+41.0266 at N-term] 2433 309 – 320 531.5347 1591.5822 1591.5974 -9.55 2 60 1 R.DIYKDADEDMRR.A + Phospho (Y); [-14.0520 at K4] 2434 309 – 320 531.5347 1591.5824 1591.5974 -9.44 2 60 1 R.DIYKDADEDMRR.A + Phospho (Y); [-14.0520 at K4] 836 311 – 319 571.7419 1141.4692 1141.4710 -1.50 1 79 1 I.YKDADEDMR.R 837 311 – 319 571.7421 1141.4696 1141.4710 -1.15 1 72 1 I.YKDADEDMR.R 839 311 – 319 571.7427 1141.4709 1141.4710 -0.066 1 83 1 I.YKDADEDMR.R 215 313 – 319 426.1644 850.3143 850.3127 1.89 0 66 0.00097 1 K.DADEDMR.R

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 217 313 – 319 426.1645 850.3144 850.3127 2.05 0 65 0.00098 1 K.DADEDMR.R 219 313 – 319 426.1646 850.3146 850.3127 2.24 0 66 0.00096 1 K.DADEDMR.R 221 313 – 319 426.1647 850.3149 850.3127 2.62 0 62 0.0022 1 K.DADEDMR.R 223 313 – 319 426.1649 850.3153 850.3127 3.06 0 66 0.00096 1 K.DADEDMR.R 1542 328 – 339 668.3306 1334.6467 1334.6466 0.068 0 75 0.00025 1 U R.ESNGTVLSTNWK.D 1543 328 – 339 668.3312 1334.6478 1334.6466 0.91 0 73 0.00038 1 U R.ESNGTVLSTNWK.D 1544 328 – 339 668.3315 1334.6484 1334.6466 1.30 0 73 0.00039 1 U R.ESNGTVLSTNWK.D 1546 328 – 339 668.8207 1335.6268 1335.6500 -17.3 0 72 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1547 328 – 339 668.8216 1335.6285 1335.6500 -16.1 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1548 328 – 339 668.8228 1335.6311 1335.6500 -14.2 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1549 328 – 339 668.8233 1335.6320 1335.6500 -13.5 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1550 328 – 339 668.8233 1335.6320 1335.6500 -13.5 0 73 1 U R.ESNGTVLSTNWK.D + [+1.0034 at N3] 1552 328 – 339 669.3163 1336.6181 1336.6147 2.55 0 72 1 U R.ESNGTVLSTNWK.D + 2 [+0.9840 at N3,N10] 1553 328 – 339 669.3175 1336.6204 1336.6534 -24.7 0 59 1 U R.ESNGTVLSTNWK.D + 2 [+1.0034 at S2,N3] 2970 340 – 357 634.3134 1899.9184 1899.9248 -3.35 1 56 5 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2971 340 – 357 634.3155 1899.9247 1899.9248 -0.046 1 107 1 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2972 340 – 357 634.3156 1899.9251 1899.9248 0.14 1 86 1 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2973 340 – 357 634.3161 1899.9265 1899.9248 0.90 1 85 2 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2974 340 – 357 634.3163 1899.9270 1899.9248 1.19 1 78 1 U K.DVGSKTVEASPPDGMELK.K + [+41.0265 at K5] 2983 340 – 357 639.6458 1915.9156 1915.9197 -2.13 1 113 1 U K.DVGSKTVEASPPDGMELK.K + [+57.0215 at S4] 2984 340 – 357 639.6468 1915.9186 1915.9197 -0.56 1 102 1 U K.DVGSKTVEASPPDGMELK.K + [+57.0215 at S4] 1592 345 – 357 679.3278 1356.6411 1356.6409 0.15 0 61 1 U K.TVEASPPDGMELK.K + [-16.0135 at M10] 1594 345 – 357 679.3281 1356.6417 1356.6409 0.60 0 52 1 U K.TVEASPPDGMELK.K + [-16.0135 at M10] 1821 345 – 357 458.5581 1372.6525 1372.6544 -1.40 0 71 0.00065 1 U K.TVEASPPDGMELK.K 1824 345 – 357 687.3339 1372.6532 1372.6544 -0.85 0 104 3.4e-07 1 U K.TVEASPPDGMELK.K 1825 345 – 357 687.3339 1372.6533 1372.6544 -0.81 0 105 2.5e-07 1 U K.TVEASPPDGMELK.K 1826 345 – 357 687.3340 1372.6534 1372.6544 -0.74 0 113 4.4e-08 1 U K.TVEASPPDGMELK.K 1827 345 – 357 687.3340 1372.6534 1372.6544 -0.71 0 109 9.4e-08 1 U K.TVEASPPDGMELK.K 1829 345 – 357 687.3342 1372.6538 1372.6544 -0.46 0 110 8.8e-08 1 U K.TVEASPPDGMELK.K 1830 345 – 357 687.3342 1372.6539 1372.6544 -0.36 0 106 2.2e-07 1 U K.TVEASPPDGMELK.K 1831 345 – 357 687.3345 1372.6544 1372.6544 -0.037 0 110 8.9e-08 1 U K.TVEASPPDGMELK.K 1832 345 – 357 687.3345 1372.6544 1372.6544 -0.037 0 98 1.4e-06 1 U K.TVEASPPDGMELK.K 1833 345 – 357 687.3345 1372.6544 1372.6544 -0.037 0 76 0.00021 1 U K.TVEASPPDGMELK.K 1834 345 – 357 687.3346 1372.6547 1372.6544 0.18 0 97 1.8e-06 1 U K.TVEASPPDGMELK.K 1837 345 – 357 687.3347 1372.6549 1372.6544 0.34 0 109 9.1e-08 1 U K.TVEASPPDGMELK.K 1838 345 – 357 687.3348 1372.6550 1372.6544 0.43 0 105 2.5e-07 1 U K.TVEASPPDGMELK.K 1839 345 – 357 687.3350 1372.6555 1372.6544 0.76 0 110 9e-08 1 U K.TVEASPPDGMELK.K 1840 345 – 357 687.3350 1372.6555 1372.6544 0.78 0 110 7.8e-08 1 U K.TVEASPPDGMELK.K 1841 345 – 357 687.3351 1372.6555 1372.6544 0.82 0 91 6.4e-06 1 U K.TVEASPPDGMELK.K 1842 345 – 357 687.3353 1372.6560 1372.6544 1.16 0 91 6.4e-06 1 U K.TVEASPPDGMELK.K 1843 345 – 357 687.3355 1372.6564 1372.6544 1.45 0 63 0.0042 1 U K.TVEASPPDGMELK.K 1844 345 – 357 687.3355 1372.6565 1372.6544 1.51 0 55 0.025 1 U K.TVEASPPDGMELK.K 1845 345 – 357 687.3371 1372.6597 1372.6544 3.87 0 86 2.1e-05 1 U K.TVEASPPDGMELK.K 1847 345 – 357 687.3384 1372.6623 1372.6544 5.72 0 56 0.021 1 U K.TVEASPPDGMELK.K 1909 345 – 357 695.3303 1388.6461 1388.6493 -2.30 0 75 0.00026 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1910 345 – 357 695.3305 1388.6465 1388.6493 -2.05 0 81 6.2e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1912 345 – 357 695.3318 1388.6490 1388.6493 -0.27 0 82 5.3e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M)

Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1913 345 – 357 695.3318 1388.6490 1388.6493 -0.24 0 70 0.00096 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1914 345 – 357 695.3318 1388.6491 1388.6493 -0.19 0 78 0.00014 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1915 345 – 357 695.3319 1388.6493 1388.6493 -0.050 0 79 0.00012 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1917 345 – 357 695.3326 1388.6506 1388.6493 0.91 0 84 3.7e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1918 345 – 357 695.3328 1388.6510 1388.6493 1.17 0 75 0.00025 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1919 345 – 357 695.3328 1388.6511 1388.6493 1.29 0 81 7e-05 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1921 345 – 357 695.3332 1388.6519 1388.6493 1.84 0 62 0.0059 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1922 345 – 357 695.3340 1388.6534 1388.6493 2.95 0 54 0.037 1 U K.TVEASPPDGMELK.K + Oxidation (M) 1930 345 – 357 465.8811 1394.6216 1394.6364 -10.6 0 65 1 U K.TVEASPPDGMELK.K + [+21.9819 at D8] 1932 345 – 357 698.3229 1394.6312 1394.6364 -3.71 0 65 1 U K.TVEASPPDGMELK.K + [+21.9819 at D8] 1978 345 – 357 706.3225 1410.6304 1410.6313 -0.59 0 58 1 U K.TVEASPPDGMELK.K + Oxidation (M); [+21.9819 at E11] 1984 345 – 357 708.8372 1415.6598 1415.6602 -0.30 0 100 1 U K.TVEASPPDGMELK.K + [+43.0058 at M10] 1985 345 – 357 708.8380 1415.6615 1415.6602 0.89 0 71 1 U K.TVEASPPDGMELK.K + [+43.0058 at M10] 2014 345 – 357 715.8465 1429.6785 1429.6759 1.83 0 77 1 U K.TVEASPPDGMELK.K + Oxidation (M); [+41.0266 at N-term]

· Web view1747.8383 1747.8377 0.34 0 145 1 Y.TQAIDAGPATADLYADR.A 2112 27 – 43 583.6201...

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