Supplemental Lian139014_Fig S1 Vector

YAP-WT

YAP-5SA

0

100

200

300

400

500

Control YAP-WT YAP-5SA

A.

B.

C.

CON

Vector

WT

YAP-WT mouse

Active

YAP-5SA mouse

0

250

500

750

1000

5550

5700

Tera

tom

a m

ass (

mg

)

Vector

YAP-WT mouse

YAP-5SA mouse

D.O

ct4

-GF

P+

Colo

nie

s

Solexa chip-seq output

Filter for 5’UTR & TSS fragments

Filter for TEAD binding sites

Implication of functional roles in ES (Genecard/Gene Ontology database)

Filter for 5’UTR & TSS fragments

Gene

Ontology

(GO)

Analysis

14125 hits

7098 gene

5038 hits

3052 gene

887 hits

640 gene

334 hits

216 gene

176 hits

(19.8%)51 hits

(15.3%)

Pluripotent ES Differntiated ES

Compare with undiff. ES dataCandidate YAP

targets (32) w.

pluripotent

implications

Filter for TEAD binding sites

Supplemental Lian139014_Fig S2

A.

C.

GAPDH Ncoa1 Cugbp2 Sertad2 Tbpl1 Rgs17

LIFr Ifnar2 Smad7 Smad2 Ppplr13 Zbtb17

Dnmt3l Tead4 Hexim Bmpr1b Mgat1 Cbfa2t3

Function # of framents TEAD site

BMP receptor 1b 1 Yes

ET02, transcription repressor 2 Yes

Cyclin, type D3 1 Yes

silincing tissue specific mRNA 1 Yes

tran. Repression via histone deacetylase 1 1 Yes

Required for GTPase activity 2 Yes

histone deacetylase 5 1 Yes

RNA polymerase II inhibitor 1 Yes

IF beta receptor 2, activates JAK1 & Stat pathway 1 Yes

completely inhibits IRF1-mediated transcription 2 Yes

Ecat1, ES cell associated protein 1 1 Yes

LIF receptor 1 Yes

key regulator for embryogenesis process 1 Yes

Mysoin VIIa & Rab interacting protein 1 Yes

coactivator mediated by Stat3, 5 &6 2 Yes

invovled in lens dvelopment 1 Yes

IASPP1, inhibitor of P53, NF-Kappa B & SP1 1 Yes

regulation of gorwth, diff, mitotic cycle and oncogenic formation 2 Yes

member of Ras oncology family 1 Yes

inhibits signal transduction by increase GTPase actiivty 1 Yes

RNA polymerase II associated proteins 3 Yes

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD)1 Yes

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma1 Yes

Antagonist of signaling by TGF- 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access1 Yes

key regulator for dev. Regulated genes 2 Yes

required for mesoderm differentitation 1 Yes

transcriptional enhancer factor (TEF) family 1 Yes

inhibits mitotic spindle assmebly & nuclear envelope breakdown 1 Yes

regulation of cell development & growth 1 Yes

regulation of cell development & growth 1 Yes

Stablizing apitcal membrane through AUM/Cytoskeletal interactions 1 Yes

Req. for early embryonic dev druing gastulation 1 Yes

B.

1 2 3 4

GAPDH

TEAD4

LIFr1

Smad2

BMPr1b

Mgat1

Ncoa1

Tbpl1

D.

Supplemental Lian139014_Fig S3