UC Davis/NIH NeuroMab Facility - Welcome to …neuromab.ucdavis.edu/datasheet/N152B_23.pdf · UC...

1
UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519 http://neuromab.ucdavis.edu [email protected] (530) 752-8398 A cooperative venture between the University of California at Davis, the National Institutes of Health and Antibodies Incorporated Copyright © 2017 The Regents of the University of California All Rights Reserved Anti-VDAC1, NeuroMab clone N152B/23 TC supe catalog # 73-204 (RRID:AB_10673517) & Pure IgG catalog # 75-204 (RRID:AB_2214807) Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1 (also known as Voltage-dependent anion-selective channel protein 1, VDAC, VDAC5, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL/M, accession number P21796) Mouse: 98% identity (279/283 amino acids identical) Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3 Monoclonal antibody info: Mouse strain: Balb/C Myeloma cell: SP2/0 Mouse Ig Isotype: IgG2a NeuroMab Applications: Immunoblot, Immunocytochemistry, Immunohistochemistry and Array Tomography Species Reactivity: human, mouse, rat Does not cross-react with VDAC2 or VDAC3 (based on KO validation results) MW: 30 kDa Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine). Adult rat hippocampus immunohistochemistry Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).

Transcript of UC Davis/NIH NeuroMab Facility - Welcome to …neuromab.ucdavis.edu/datasheet/N152B_23.pdf · UC...

UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519 http://neuromab.ucdavis.edu [email protected] (530) 752-8398

A cooperative venture between the University of California at Davis, the National Institutes of Health and Antibodies Incorporated Copyright © 2017 The Regents of the University of California

All Rights Reserved

Anti-VDAC1, NeuroMab clone N152B/23 TC supe catalog # 73-204 (RRID:AB_10673517) & Pure IgG catalog # 75-204 (RRID:AB_2214807) Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1 (also known as Voltage-dependent

anion-selective channel protein 1, VDAC, VDAC5, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL/M, accession number P21796)

Mouse: 98% identity (279/283 amino acids identical) Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3 Monoclonal antibody info: Mouse strain: Balb/C Myeloma cell: SP2/0 Mouse Ig Isotype: IgG2a NeuroMab Applications: Immunoblot, Immunocytochemistry, Immunohistochemistry and Array Tomography Species Reactivity: human, mouse, rat Does not cross-react with VDAC2 or VDAC3 (based on KO validation results)

MW: 30 kDa Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine). Adult rat hippocampus immunohistochemistry Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).