UAB Comprehensive Flow Cytometry Core (CFCC) · UAB Comprehensive Flow Cytometry Core (CFCC) John...
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Our Vision of CFCC Technology "Gate-Way" to High Dimensional Single-Cell Omics Analysis Novel Probes • Tetramers • Dendramers • Nanoparticles • Click-IT • Enzymatic probes UAB Comprehensive Flow Cytometry Core (CFCC) John D. Mountz 1, 2 , Olaf Kutsch 3 , and Troy Randall 1 1 Division of Clinical Immunology and Rheumatology, Department of Medicine, UAB 2 Birmingham VA Medical Center, and 3 Division of Infectious Diseases, Department of Medicine, UAB CFCC at Shelby (Rm 271): Vidya Sagar Hanumanthu ([email protected] ); Ana Leda F Longhini ([email protected] ); Berencia Marie Fore ([email protected] ) CFCC at BBRB (Rm 557): Marion Spell ([email protected] ) CFCC at Shelby (Rm 271): Single Cell RNA Analysis Specialist: Shanrun Liu ([email protected] ) BD ARIA Sorter Amnis ImageStream VA ShEEP grant supported BD LSRII Analyzer The HyperCyt autosampler BioPlex suspension array system UAB Radiation Oncology Nanostring UAB Radiation Oncology Kinome Core UAB Proteomics Core RDCC Analytical Genomics and Transgenics Core UAB Heflin Genomics Core RDCC Analytic Imaging and Immunoreagents Core Technology Partners Technology Partners This Core is supported by: VA Shared Equipment Evaluation (ShEEP) Program; UAB Institutional Research Core Support; NIH/NIAMS P30-AR048311; NIH/NIAMS P30-AI27667 UAB HSF-GEF Other Birmingham VA & UAB Research Cores UAB Center for Clinical & Translational Science Genetic/Epigenetic NanoString Genome Epigenome RNA-seq Cloning of genes Protein Cytokines Phospho-protein Signalome Kinome Cell Cloning Transfection Imaging Metabolome Functional assays Single Cell RNA/Transcriptome • Fluidigm/BioMark • 10x Genomics RNA-seq • The BD Rhapsody™ Express system BD FACSMelody cell sorter Attune NxT Flow Cytometer BD FACSymphony VA ShEEP grant supported
Transcript of UAB Comprehensive Flow Cytometry Core (CFCC) · UAB Comprehensive Flow Cytometry Core (CFCC) John...
Our Vision of CFCC Technology "Gate-Way" to High Dimensional Single-Cell Omics Analysis
Novel Probes• Tetramers• Dendramers• Nanoparticles• Click-IT• Enzymatic probes
UAB Comprehensive Flow Cytometry Core (CFCC) John D. Mountz1, 2, Olaf Kutsch3, and Troy Randall1
1Division of Clinical Immunology and Rheumatology, Department of Medicine, UAB2Birmingham VA Medical Center, and 3Division of Infectious Diseases, Department of Medicine, UAB
CFCC at Shelby (Rm 271): Vidya Sagar Hanumanthu ([email protected]); Ana Leda F Longhini ([email protected]); Berencia Marie Fore ([email protected])CFCC at BBRB (Rm 557): Marion Spell ([email protected])CFCC at Shelby (Rm 271): Single Cell RNA Analysis Specialist: Shanrun Liu ([email protected])
BD ARIA Sorter
Amnis ImageStreamVA ShEEP grant supported
BD LSRII Analyzer The HyperCyt autosampler
BioPlex suspension array system
UAB Radiation Oncology
Nanostring
UAB Radiation Oncology
Kinome Core
UAB Proteomics
Core
RDCC Analytical Genomics
and Transgenics Core
UAB Heflin Genomics Core
RDCC Analytic Imaging and
Immunoreagents Core
Technology Partners Technology Partners
This Core is supported by:VA Shared Equipment Evaluation (ShEEP) Program;
UAB Institutional Research Core Support; NIH/NIAMS P30-AR048311; NIH/NIAMS P30-AI27667
UAB HSF-GEF
Other Birmingham VA & UAB Research Cores
UAB Center for Clinical &
Translational Science
Genetic/EpigeneticNanoStringGenomeEpigenomeRNA-seqCloning of genes
ProteinCytokinesPhospho-protein SignalomeKinome
CellCloningTransfectionImagingMetabolomeFunctional assays
Single Cell RNA/Transcriptome
• Fluidigm/BioMark• 10x Genomics RNA-seq• The BD Rhapsody™
Express system
BD FACSMelody cell sorter
Attune NxT Flow Cytometer
BD FACSymphony VA ShEEP grant supported
