Transonic Systems Inc. Considerations for Flow Measurement on the Mouse Femoral Artery Location of...

TransonicSystems Inc.

Considerations for Flow Measurement on the Mouse Femoral Artery

• Location of Measurement with the Flowprobe

• Vessel Diameter

• Maintaining Body Temperature

• Experimental Set-up

Photos and data courtesy of Michael F. Callahan

Depts. of Orthopaedic Surgery,

Physiology & Pharmacology,

Wake Forest University Health Sciences

Winston-Salem, North Carolina

All information in this file is privileged information and may not be used without permission of the author.


Femoral Nerve,Artery and Vein exiting from abdominal wall

Epigastric Nerve,Artery and Vein

Femoral Nerve, Superficial Femoral Artery and Vein

Branches of Femoral Artery Before and After Dissection

Approximate Location of Knee



Femoral artery proximal to the epigastric a., isolated from the

femoral vein, measures ~ 400 µm diameter with a micrometer.

Flow measurements can be made in this location easily.



Femoral Blood Flow in Anesthetized Mouse Recorded with Transonic Systems’ Flowmeter & Nanoprobe

MFCallahan, 3/03

Data Courtesy of Michael F. Callahan, Wake Forest Health Sciences, Winston-Salem, NC



39°C

0.82 ml/m

37°C

0.64 ml/m

35°C

0.51 ml/m

33°C

0.27 ml/m

Effect of Core Temperature on Femoral Blood Flow (22g CD-1, 3-16-03)

Data Courtesy of Michael F. Callahan, Wake Forest Health Sciences, Winston-Salem, NC

Temperature has a profound effect on femoral blood flow and must be monitored.


Flow measurements on the femoral artery distal to the epigastric a.

Isolation of the artery from the vein in this position is very challenging. There is less fascia and connective tissue around the sheath of the artery and vein to manipulate the vessel free.

The nerve runs immediately adjacent and is very easy to tear.

Once isolated, avoid pulling up on the artery which would cause vascular spasm or putting pressure on the vein to cause occlusion.

Superficial femoral a.


The superficial femoral artery, distal to the epigastric a., is isolated from the femoral vein and

measures ~ 200 µm diameter on the scale.


Transonic 0.5PSB flowprobe on the superficial femoral artery distal to the epigastric a.


32g male CD-1 with 0.5PSB probe on superficial femoral artery

Temperature 33o C.

Michael Callahan, Wake Forest Univ. Health Sciences, 2/5/2004

Sample flow trace from lower on femoral artery near the knee. Since the vessel diameter fills < 60% of the probe lumen, probe position must remain fixed for a stable measurement.


Experimental Setup for Femoral Artery Blood Flow Study

Close up of the Transonic nanoprobe handle clamped in a micromanipulator with two axis adjustment to fine tune and stabilize position of probe for measurement.


Experimental Setup for Femoral Artery Blood Flow Study

Probe holder with magnetic base, adjustable arm, and micro-positioning adjustment

Heating pad & lamp used with gauze cover to maintain mouse core temperature. Temp. is monitored with an anal probe.

TransonicSystems 400-Series Flowmeter Console


Simultaneous Femoral Artery Blood Flow & Pressure Recording


Flow recorded from the superficial femoral a. with occlusion; Pressure recorded from the contra lateral femoral a.


Transonic Systems, Inc. gratefully acknowledges the investigators for the presentation of this data on the Transonic website.

All information in this file is privileged information and may not be used without permission of the authors.

Photos and data courtesy of :

Michael F. Callahan, PhD

Thomas L. Smith, PhD

Depts. of Orthopaedic Surgery,

Physiology & Pharmacology,

Wake Forest University Health Sciences

Winston-Salem, North Carolina

Edited by Margo Sosa, Sr. Product Manager

Transonic Systems, Inc.

www.transonic.com

December 2004

Transonic Systems Inc. Considerations for Flow Measurement on the Mouse Femoral Artery Location of...

Documents

Transcript of Transonic Systems Inc. Considerations for Flow Measurement on the Mouse Femoral Artery Location of...