― 25 ―

Training Courses

【2005】

“Training program of adenovirus vector”Overview: The advanced technology of adenovirus vector

is widely used and one of the genetic tools for the research

of gene delivery. We performed the training program for the

scientist who are not familiar with techniques of production,

purification and infection of recombinant adenoviruses

(rAds). In this course, we focused on (i) how to construct the

adenovirus shuttle vector and produce the adenovirus particle

and (ii) the rAd mediated gene transfer into the eukaryotic

cells and the animal model. We also gave a lecture entitled as

the safety and use of adenovirus vector and an overview of the

preparation of adenovirus vector.

Host division: Gene Engineering Division

Term of course: Aug. 24~26, 2005 (3 days)

Number of trainee: 3

“Training course on cultivation and preservation of strictly anaerobic microbes”Overview: The course provided beginners cultivation and

archaea and sulfate-reducing bacteria.

Host division: Microbe Division

Term of course: Aug. 29~30, 2005 (2 days)

Number of trainee: 4

“Technical training course for cryopreservation of mouse sperm and embryos”Overview: For researchers and technicians manipulating

mouse embryos, we held an open training course to teach

essential techniques for in vitro fertilization and embryo/

sperm cryopreservation. Additionally, we showed them how to

perform intracytoplasmic sperm injection and nuclear transfer

cloning using micromanipulators.

Host division: Bioresource Engineering Division

Term of course: Oct. 11~14, 2005 (4 days)

Number of trainee: 3

“Training courses on advanced techniques for plant cell lines”

Overview: Cryopreservation of Arabidopsis T87 cell and

tobacco BY-2 cell (Course A), and transformation and

cryopreservation of Arabidopsis T87 cell (Course B).

Host division: Experimental Plant Division

Term of course: (Course A) Nov. 7~8, 2005 (2 days)

(Course B) Nov. 8~9, 2005 (2 days)

Number of trainee: 4 each (Total 8)

“Training course on culture-independent analysis of human gut microbiota by terminal-RFLP”Overview: The course provided analysis of human gut

microbiota by terminal-RFLP.

Host division: Microbe Division

Term of course: Dec. 13~15, 2005 (3 days)

Number of trainee: 10

【2006】

“Train ing course on transformat ion and preservation of plant cell lines”Overview: Par t 1 , cu l tu re techniques needed for

transformation and cryopreservation; Part 2, efficient protocol

for transformation; Part 3, protocol for cryopreservation.

Host division: Experimental Plant Division

Term of course: Jul. 25~26, 2006 (2 days)

Number of trainee: 4

“ Tr a i n i n g c o u r s e o n m a i n t e n a n c e a n d transformation of plant cell lines”Overview: Part 1, culture techniques for tobacco BY-2

and Arabidopsis T87 cells; Part 2, efficient protocol for

transformation.

Host division: Experimental Plant Division

Term of course: Sep. 5~6, 2006 (2 days)

Number of trainee: 5

Training CoursesRIKEN BRC Annual Report 2005 ～ 2007

― 26 ―

“Training program of adenovirus vector”

Overview: The advanced technology of adenovirus vector

is widely used and oneof the genetic tools for the research of

gene delivery. We performed the training program for the

scientist who are not familiar with techniques of production,

purification and infection of recombinant adenoviruses

(rAds). In this course, we focused on (i) how to construct the

adenovirus shuttle vector and produce the adenovirus particle

and (ii) the rAd mediated gene transfer into the eukaryotic

cells and the animal model. We also gave a lecture entitled as

the safety and use of adenovirus vector and an overview of the

preparation of adenovirus vector.

Host division: Gene Engineering Division

Term of course: Sep. 6~8, 2006 (3 days)

Number of trainee: 3

“Technical training course for cryopreservation of mouse sperm and embryos”Overview: For researchers and technicians manipulating

mouse embryos, we held an open training course to teach

essential techniques for in vitro fertilization and embryo/

sperm cryopreservation. Additionally, we showed them how to

perform intracytoplasmic sperm injection and nuclear transfer

cloning using micromanipulators.

Host division: Bioresource Engineering Division

Term of course: Oct. 10~13, 2006 (4 days)

Number of trainee: 4

“Training course for maintenance and quality control of genetically-engineered mice”

Overview: The course provides basic theory of breeding and

quality control of genetically-engineered mice, and practice of

genotyping of the mice using PCR machine.

Host division: Experimental Animal Division and Tsukuba

Experimental Animal Collocium

Term of course: Oct. 25, 2006 (1 day)

Number of trainee: 5

“Train ing course on the cu l t ivat ion and preservation of microorganisms”Overview: The course provided beginners the methods for

cultivations and preservations of bacteria, archaea, yeasts, and

fidamentous fungi.

Host division: Microbe Division

Term of course: Jan. 22~23, 2007 (2 days)

Number of trainee: 6

【2007】

“ Tr a i n i n g c o u r s e o n m a i n t e n a n c e a n d transformation of Arabidopsis T87 cell”Overview: Part 1, culture techniques for Arabidopsis T87

cell; Part 2, efficient protocol for transformation.

Host division: Experimental Plant Division

Term of course: Jul. 23~24, 2007 (2 days)

Number of trainee: 5

Training CoursesRIKEN BRC Annual Report 2005 ～ 2007

― 27 ―

“Training course on preservation of tobacco BY-2 cell”

Overview: Par t 1 , cu l tu re techniques needed for

transformation and cryopreservation; Part 2, protocol for

cryopreservation.

Host division: Experimental Plant Division

Term of course: Aug. 10, 2007 (1 day)

Number of trainee: 6

“Handling and maintenance of model plant Arabidopsis”Overview: Part 1, techniques needed for Arabidopsis

cultivation; Part 2, protocol for transformation; Part 3,

protocol for crossbreeding..

Host division: Experimental Plant Division

Term of course: Sep. 4~5, 2007 (2 days)

Number of trainee: 2

“Technical training course for cryopreservation of mouse sperm and embryos”Overview: For researchers and technicians manipulating

mouse embryos, we held an open training course to teach

essential techniques for in vitro fertilization and embryo/

sperm cryopreservation. Additionally, we showed them how to

perform intracytoplasmic sperm injection and nuclear transfer

cloning using micromanipulators.

Host division: Bioresource Engineering Division

Term of course: Oct. 9~12, 2007 (4 days)

Number of trainee: 3

“Training program of adenovirus vector”

Overview: The advanced technology of adenovirus vector

is widely used and one of the genetic tools for the research

of gene delivery. We performed the training program for the

scientist who are not familiar with techniques of production,

purification and infection of recombinant adenoviruses

(rAds). In this course, we focused on (i) how to construct the

adenovirus shuttle vector and produce the adenovirus particle

and (ii) the rAd mediated gene transfer into the eukaryotic

cells and the animal model. We also gave a lecture entitled as

the safety and use of adenovirus vector and an overview of the

preparation of adenovirus vector.

Host division: Gene Engineering Division

Term of course: Oct. 23~26, 2007 (4 days)

Number of trainee: 3

“Training course on culture-independent analysis of human gut microbiota by terminal-RFLP”

Overview: The course provided analysis of human gut

microbiota by terminal-RFLP.

Host division: Microbe Division

Term of course: Feb 26~27, 2008 (2 days)

Number of trainee: 8

【2008】

“ Tr a i n i n g c o u r s e o n m a i n t e n a n c e a n d transformation of Arabidopsis T87 cell”Overview: Part 1, culture techniques for Arabidopsis T87

cell; Part 2, efficient protocol for transformation.

Host division: Experimental Plant Division

Term of course: Aug. 5~6, 2008 (2 days)

Number of trainee: 2

“Training course on preservation of tobacco BY-2 cell”Overview: Par t 1 , cu l tu re techniques needed for

transformation and cryopreservation; Part 2, protocol for

cryopreservation.

Host division: Experimental Plant Division

Term of course: Aug. 25~26, 2008 (2 days)

Number of trainee: 6

Training CoursesRIKEN BRC Annual Report 2005 ～ 2007

― 28 ―

“Handling and maintenance of model plant Arabidopsis”

Overview: Part 1, techniques needed for Arabidopsis

cultivation; Part 2, protocol for transformation; Part 3,

protocol for crossbreeding.

Host division: Experimental Plant Division

Term of course: Sep. 4~5, 2008 (2 days)

Number of trainee: 3

“Technical training course for cryopreservation of mouse sperm and embryos”Overview: For researchers and technicians manipulating

mouse embryos, we held an open training course to teach

essential techniques for in vitro fertilization and embryo/

sperm cryopreservation. Additionally, we showed them how to

perform intracytoplasmic sperm injection and nuclear transfer

cloning using micromanipulators.

Host division: Bioresource Engineering Division

Term of course: Oct. 20~23, 2008 (4 days)

Number of trainee: 3

“Training program of adenovirus vector”Overview: The advanced technology of adenovirus vector

is widely used and one of the genetic tools for the research

of gene delivery. We performed the training program for the

scientist who are not familiar with techniques of production,

purification and infection of recombinant adenoviruses

(rAds). In this course, we focused on (i) how to construct the

adenovirus shuttle vector and produce the adenovirus particle

and (ii) the rAd mediated gene transfer into the eukaryotic

cells and the animal model. We also gave a lecture entitled as

the safety and use of adenovirus vector and an overview of the

preparation of adenovirus vector.

Host division: Gene Engineering Division

Term of course: Oct. 22~24, 2008 (3 days)

Number of trainee: 3