TM Bidentate C18 NOTES - mtc-usa.com • MicroSolv Technology Corporation 1 Industrial Way West,...
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40018-25P Cogent Bidentate C18 ™ HPLC Column, 100Å, 4μm, 4.6 x 250 mm. Cat. No. Description APP A06-10-A34 APPLICATION NOTES MTC • MicroSolv Technology Corporation 1 Industrial Way West, Bldg E, Eatontown, NJ 07724 USA Ph. 1-732-578-1777 • Fx. 1-732-578-9777 www.mtc-usa.com Email: [email protected] Method Conditions Column: Cogent Bidentate C18, 4μm, 100Å. Catalog No.: 40018-25P Dimensions: 4.6 x 250 mm Mobile phase: 100% DI water + 0.5% formic acid Flow rate: 0.3 mL/min. Injection Volume: 10 μL Peaks/Samples: 1. Maltotriose α 1,4 m/z 144 2. Panose α 1,4 + α 1,6 m/z 127 3. Isomaltotriose α 1,6 m/z 127 4. Cellotriose β 1,4 m/z 144 Sample Concentration: 0.1 mg of each in DI water All compounds have MW = 504. Detection: APCI + . Single Ion Monitoring Discussion The separation presented offers an easy and reproducible analytical method for the analysis of positional isomers of oligosaccharides. Two additional components (shoulders on the peaks 3 and 4) have yet to be identified. Despite using 100% water as the mobile phase, the analysis was very reproducible and no loss of retention over time was observed from run to run. Some of the most important actions of oligosaccharides are: inhibition of the growth of pathogenic and harmful bacteria, stimulation of the immune functions, reduction of cholesterol level and reduction of the risk of cancer and the mprovement of digestion/absorption of food ingredients such as minerals and vitamins. The structures of oligosaccharides can be very complex, which allows very specific bio-interactions. On the other hand the complexity of the structure is a limitation to the possibility of developing efficient synthesis routes. To make possible the large scale applications of oligosaccharides in various fields (agrochemistry, cosmetics, food and feed, drug target, inflammatory phenomena, immuno-stimulation, etc.,) scientists will need synthesis methods and then confirmation of the purity of the products while in-process. Oligosaccharides are usually synthesized by enzymes which takes a long time and the determination of the purity of the products is difficult. For more information visit www.MTC-USA.com Bidentate C18 TM Carbohydrates Positional Isomer Analysis by LCMS Note: :Compounds 1-4 were also analyzed using a leading brand of C18 columns (Type-B, high purity) but only 3 peaks were observed and the analysis was not reproducible. HPLC Simultaneous analysis of α and β -linked gluco-oligosaccharides.
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Transcript of TM Bidentate C18 NOTES - mtc-usa.com • MicroSolv Technology Corporation 1 Industrial Way West,...
40018-25P Cogent Bidentate C18™ HPLC Column, 100Å, 4µm, 4.6 x 250 mm.
Cat. No. Description
APP A06-10-A34
AP
PL
ICA
TIO
NN
OT
ES
MTC • MicroSolv Technology Corporation1 Industrial Way West, Bldg E, Eatontown, NJ 07724 USAPh. 1-732-578-1777 • Fx. 1-732-578-9777www.mtc-usa.com Email: [email protected]
Method Conditions
Column: Cogent Bidentate C18, 4µm, 100Å.Catalog No.: 40018-25P Dimensions: 4.6 x 250 mm Mobile phase: 100% DI water + 0.5% formic acid Flow rate: 0.3 mL/min.Injection Volume: 10 µLPeaks/Samples: 1. Maltotriose α 1,4 m/z 144
2. Panose α 1,4 + α 1,6 m/z 127 3. Isomaltotriose α 1,6 m/z 127 4. Cellotriose β 1,4 m/z 144
Sample Concentration: 0.1 mg of each in DI waterAll compounds have MW = 504.
Detection: APCI+. Single Ion Monitoring
DiscussionThe separation presented offers an easy and reproducible analyticalmethod for the analysis of positional isomers of oligosaccharides. Twoadditional components (shoulders on the peaks 3 and 4) have yet tobe identified. Despite using 100% water as the mobile phase, theanalysis was very reproducible and no loss of retention over time wasobserved from run to run.
Some of the most important actions of oligosaccharides are: inhibition of thegrowth of pathogenic and harmful bacteria, stimulation of the immunefunctions, reduction of cholesterol level and reduction of the risk of cancer andthe mprovement of digestion/absorption of food ingredients such as mineralsand vitamins. The structures of oligosaccharides can be very complex, whichallows very specific bio-interactions. On the other hand the complexity of thestructure is a limitation to the possibility of developing efficient synthesis routes.To make possible the large scale applications of oligosaccharides in variousfields (agrochemistry, cosmetics, food and feed, drug target, inflammatoryphenomena, immuno-stimulation, etc.,) scientists will need synthesis methodsand then confirmation of the purity of the products while in-process.Oligosaccharides are usually synthesized by enzymes which takes a long timeand the determination of the purity of the products is difficult.
For more information visit www.MTC-USA.com
Bidentate C18
TM
Carbohydrates Positional Isomer Analysis by LCMS
Note: :Compounds 1-4 were also analyzed using aleading brand of C18 columns (Type-B, high purity)but only 3 peaks were observed and the analysis wasnot reproducible.
HPLC
Simultaneous analysis of αα and ββ -linked
gluco-oligosaccharides.
