Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
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Transcript of Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
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Type 1 Diabetes Melitus
Induction (streptozocin based)
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Diabetes
• Diabetes is a chronic disease, which occurs
when the pancreas does not produce
enough insulin, or when the body cannot
effectively use the insulin it produces. This
leads to an increased concentration of
glucose in the blood (hyperglycaemia)
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Diabetes
Diabetes
Type 1
diabetes
Type 2
diabetes
Gestational
diabetes
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Diabetes mellitus Type 1
• Type 1 diabetes (previously known as
insulin-dependent or childhood-onset
diabetes) is characterized by a lack of
insulin production.
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ANIMAL MODEL OF DIABETES(STREPTOZOCIN BASED)
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Streptozocin (STZ)
• Isolated from Streptomyces achromogenes
• Its diabetogenic properties characterized by selective destruction
of pancreatic islet β-cells mimic human type 1 diabetes mellitus
• Several species, including the mouse, rat, rabbit, and monkey, are
sensitive to the pancreatic β-cell cytotoxic effects of STZ
C8H15N3O7
Mol. Weight : 265,22 g/mol
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STZ
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Animal
STZ
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Inducing Diabetes
• Basic Protocol 1 (mice)• multiple administrations of low-dose STZ to
produce diabetic mice
• close resemblance to human T1DM with chronicpancreatic islet inflammation, insulitis, and insulin
deficiency
• The cost effectiveness related to the small size of the
animal
• Basic Protocol 2 (rat)
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Inducing Diabetes
• Basic Protocol 1 (mice)
• Basic Protocol 2 (rat)• Not used as commonly as the mouse model
• Using a single, high dose Streptozocin
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BASIC PROTOCOL 1
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Material
• C57BL/6 or CD-1 male mice: ∼ 25 g, 8 to 12 weeksold
• 50 mM sodium citrate buffer, pH 4.5
•
Streptozotocin• 10% (w/v) sucrose
• 1.5-ml microcentrifuge tubes
• Aluminum foil
• 1-ml syringes• 25-G needles
• One Touch Basic blood glucose monitoring system
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Animal Preparation
4 dayprior
remove
food
Day 1
Treat withSTZ
2-5consecutiveday
Repeattreating
with STZ
Day 6Switch
sucrose to
regular
water
Day 14
Test bloodglucose
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Streptozocin Treatment Preparation
• Prepare 50 mM sodium citrate buffer, place
1ml into 1.5-ml microcentrifuge tube. Cover
tube with aluminium foil
• Dissolve STZ in the 50mM sodium citrate to
final conc. 6 mg/ml
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Streptozocin Treatment
• Inject STZ sol. IP at 40 mg/kg
• Return mice to their cage. Provide normal
food and 10% sucrose water
• Repeat treatment for next 4 consecutive day
• On day 6, switch the 10% sucrose with
regular water
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Diabetic State
• On experimental day 14 (9 days after STZ
injection) fast mice for 6 hr. test the blood
glucose
• Fasting glucose >150 mg/dl
• For retest of group failing of the first test,
test the blood glucose on day 28
• Severe diabetes develop 3 wk after injection