Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
-
Upload
wira-winardi -
Category
Documents
-
view
221 -
download
0
Transcript of Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
![Page 1: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/1.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 1/16
Type 1 Diabetes Melitus
Induction (streptozocin based)
![Page 2: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/2.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 2/16
Diabetes
• Diabetes is a chronic disease, which occurs
when the pancreas does not produce
enough insulin, or when the body cannot
effectively use the insulin it produces. This
leads to an increased concentration of
glucose in the blood (hyperglycaemia)
![Page 3: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/3.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 3/16
Diabetes
Diabetes
Type 1
diabetes
Type 2
diabetes
Gestational
diabetes
![Page 4: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/4.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 4/16
Diabetes mellitus Type 1
• Type 1 diabetes (previously known as
insulin-dependent or childhood-onset
diabetes) is characterized by a lack of
insulin production.
![Page 5: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/5.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 5/16
ANIMAL MODEL OF DIABETES(STREPTOZOCIN BASED)
![Page 6: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/6.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 6/16
Streptozocin (STZ)
• Isolated from Streptomyces achromogenes
• Its diabetogenic properties characterized by selective destruction
of pancreatic islet β-cells mimic human type 1 diabetes mellitus
• Several species, including the mouse, rat, rabbit, and monkey, are
sensitive to the pancreatic β-cell cytotoxic effects of STZ
C8H15N3O7
Mol. Weight : 265,22 g/mol
![Page 7: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/7.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 7/16
STZ
![Page 8: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/8.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 8/16
Animal
STZ
![Page 9: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/9.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 9/16
Inducing Diabetes
• Basic Protocol 1 (mice)• multiple administrations of low-dose STZ to
produce diabetic mice
• close resemblance to human T1DM with chronicpancreatic islet inflammation, insulitis, and insulin
deficiency
• The cost effectiveness related to the small size of the
animal
• Basic Protocol 2 (rat)
![Page 10: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/10.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 10/16
Inducing Diabetes
• Basic Protocol 1 (mice)
• Basic Protocol 2 (rat)• Not used as commonly as the mouse model
• Using a single, high dose Streptozocin
![Page 11: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/11.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 11/16
BASIC PROTOCOL 1
![Page 12: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/12.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 12/16
Material
• C57BL/6 or CD-1 male mice: ∼ 25 g, 8 to 12 weeksold
• 50 mM sodium citrate buffer, pH 4.5
•
Streptozotocin• 10% (w/v) sucrose
• 1.5-ml microcentrifuge tubes
• Aluminum foil
• 1-ml syringes• 25-G needles
• One Touch Basic blood glucose monitoring system
![Page 13: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/13.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 13/16
Animal Preparation
4 dayprior
remove
food
Day 1
Treat withSTZ
2-5consecutiveday
Repeattreating
with STZ
Day 6Switch
sucrose to
regular
water
Day 14
Test bloodglucose
![Page 14: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/14.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 14/16
Streptozocin Treatment Preparation
• Prepare 50 mM sodium citrate buffer, place
1ml into 1.5-ml microcentrifuge tube. Cover
tube with aluminium foil
• Dissolve STZ in the 50mM sodium citrate to
final conc. 6 mg/ml
![Page 15: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/15.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 15/16
Streptozocin Treatment
• Inject STZ sol. IP at 40 mg/kg
• Return mice to their cage. Provide normal
food and 10% sucrose water
• Repeat treatment for next 4 consecutive day
• On day 6, switch the 10% sucrose with
regular water
![Page 16: Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx](https://reader030.fdocuments.in/reader030/viewer/2022020723/577cd6d01a28ab9e789d5185/html5/thumbnails/16.jpg)
7/27/2019 Tipe 1 Diabetes Melitus Induction (streptozicin based.pptx
http://slidepdf.com/reader/full/tipe-1-diabetes-melitus-induction-streptozicin-basedpptx 16/16
Diabetic State
• On experimental day 14 (9 days after STZ
injection) fast mice for 6 hr. test the blood
glucose
• Fasting glucose >150 mg/dl
• For retest of group failing of the first test,
test the blood glucose on day 28
• Severe diabetes develop 3 wk after injection