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Experts In Microplate Analysis

DYNEX Technologies, Inc.14340 Sullyfield Circle, Chantilly, VA 20151-1621Phone: (703) 631-7800 FAX: (703) 803-1441www.dynextechnologies.com

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Rev. 10-15-04

OperatingManual

for MRW (AM60)

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AM60 Multi-Reagent

Washer

Operating Manual

IMPORTANT Read carefully before service or adjustment

Version: 10-15-2004 992-0060-99

© This document is the copyright of DYNEX Technologies, Inc. and must not be reproduced in any form without prior consent. DYNEX reserves the right to make technical improvements to this equipment and documentation without notice as part of a continuous programme of product development. This manual supersedes all previous editions.

MULTI-REAGENT WASHER SAFETY INFORMATION

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SAFETY INFORMATION WARNING: IF THIS EQUIPMANT IS USED IN A MANNER NOT SPECIFIED BY THE MANUFACTURER, THE PROTECTION PROVIDED BY THE EQUIPMANT MAY BE IMPAIRED. WARNING: ELECTRIC SHOCK HAZARD Although this instrument is fully insulated and earthed, it is important for all users to be aware of the potential hazards of using liquids in close proximity to an electrical supply. In the event of any spillage the instrument must be isolated from the electrical supply immediately and drained. Spilt liquid should be removed as soon as possible. CAUTION: Serious damage to the instrument may occur if fluid is allowed to penetrate the case. The electrical supply MUST NOT be reconnected until the instrument has been fully inspected by a competent electrical technician or approved service engineer. WARNING: CONTAMINATION AND SPILLAGES In some operating modes (e.g. dispensing fluids or purging the system, particularly under automatic control) it is possible to overfill the wells of a microplate. This can result in spillages of liquids and/or contamination with hazardous substances. Care should be taken to ensure that overfilling does not occur. If environmentally hazardous fluids have been spilt, the instrument must first be isolated from the electrical supply and physically isolated from the working environment by placing it in a fume cupboard or similar location. Contact the nearest approved service centre for assistance, advising them of the health hazards associated with the spilt materials. DO NOT operate this equipment with the covers removed – potentially lethal voltages are contained within. DO NOT operate this equipment with the safety earth (ground) disconnected. ENSURE that the voltage and fuse ratings on the rear panel of the washer correspond to the local mains supply. ENSURE that all tubes and connections are tight and correctly positioned before switching on the washer unit. If there is any doubt or concern about the safety of the instrument contact the nearest Dynex Service Centre listed at the end of this manual.

MULTI-REAGENT WASHER SAFETY INFORMATION

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SAFETY INFORMATION This instruction manual contains some information and warnings which have to be followed by the user to ensure safe operation and to retain the apparatus in safe condition. This apparatus has been designed for indoor use. It may occasionally be subjected to temperatures between +5°C and –10°C without degradation of its safety. 1. Before switching on the apparatus make sure that it is set to the voltage of the power

supply.

2. The mains plug shall only be inserted in a socket outlet provided with a protective earth contact. The protective action must not be negated by the use of an extension cord without a protective conductor.

3. When the apparatus is connected to its supply, terminals may be live, and the opening of covers or removal of parts (except those to which access can be gained by hand) is likely to expose live parts

4. The apparatus shall be disconnected from all voltage sources before it is opened for any adjustment, replacement, maintenance or repair.

5. Any adjustment, maintenance and repair of the opened apparatus under voltage shall be avoided as far as possible and, if inevitable, shall be carried out only by a skilled person who is aware of the hazard involved.

6. Make sure that only fuses with the required rated current and of the specified type are used for replacement. The use of makeshift fuses and the short-circuiting of fuse- holders are prohibited.

7. Whenever it is likely that the protection has been impaired, the apparatus shall be made inoperative and be secured against any unintended operation.

The protection is likely to be impaired if, for example, the apparatus:

• shows visible damage

• fails to perform the intended operations

• has been subjected to prolonged storage under unfavourable conditions

• has been subjected to severe transport stresses

MULTI-REAGENT WASHER TABLE OF CONTENTS

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TABLE OF CONTENTS Safety Information i Section 1 SPECIFICATIONS 1 Section 2 INSTALLATION 5

2.1 Unpacking 5 2.2 Mains Supply Voltages 6 MRW Training Checklist 7 and 8 2.3 Rear Panel Connections 9 2.4 Site Installation 10 2.5 Tubing connections 11 2.6 Switching on 13

Section 3 OPERATING INSTRUCTIONS 14

THE MAIN MENU 14 3.1 SETUP 15 AUTO CLEAN 15 BOTTOM ASPIRATE 16 DISPENSE HEAD 17 PLATE SETUP 17 NO. OF VALVES 18 RESET RAM 19 BOTTLE SET 19 SHIPPING 19 3.2 MANUAL 20 MOVE 20 ASPIRATE 21 DISPENSE 21 PURGE 22 FINISH 22

MULTI-REAGENT WASHER TABLE OF CONTENTS

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3.3 PROGRAM 23 Assigning a Name or Number to a Test Sequence 23 Plate Type 24 Variable Columns Option 24 Wash Again Option 24 PROGRAM OPTIONS MENU 25 CREATING A PROGRAM LOOP 25 MOVE 25 ASPIRATE 26 DISPENSE 27 PURGE 28 SOAK 28 END OF LOOP 28 PROGRAM EDITING 30 TO INSPECT AN EXISTING PROGRAM 30 TO MODIFY AN EXISTING PROGRAM 31 3.4 START 34

Section 4 COMPUTER CONTROL 35 Section 5 MAINTENANCE 37

5.1 Routine Maintenance 37 5.2 Fuse Replacement 38 5.3 Error Messages 39

APPENDIX

A: Default Assay and Plate Formats B: Assay Backup/Storage Program C: Accessories

CONTACT US

MULTI-REAGENT WASHER SPECIFICATIONS

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SPECIFICATIONS The multi-reagent microplate washer is an independent programmable washer/ aspirator for use with 96-well microplates. Wash solutions are dispensed under pressure and safely aspirated by vacuum into the waste bottle. Washer operating and programming sequences follow a logical procedure through which the user is guided by a liquid crystal display screen. The system is able to accommodate two wash solutions (up to four wash solutions as a factory-fitted option), and up to 10 microplate types can be stored in memory.

Figure 1 Multi-Reagent Washer

Laboratory personnel may devise test programs to control dispense fluid volume, fluid selection, soak time, number of wash/aspirate cycles, and system purging. Programs may be saved to non-volatile memory for permanent storage. Up to 40 user-devised programs may be entered and edited via the membrane keypad and the LCD display. 5 pre-programmed wash tests are installed in the washer. The unit has been designed for simplicity of maintenance with all tubes carrying fluid external to the unit for convenient cleaning or replacing. The aspirate/dispense head covers are removable for easy head cleaning.


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The multi-reagent washer incorporates many features for flexible and convenient operation:

• logical menu-driven operation

• manual/automatic control

• permanent program storage for up to 40 programs

• 5 pre-programmed wash tests

• selection of up to four wash solutions

• choice of 2 litre or 4 litre bottles

• 8-way or 16-way floating head

• programmable wash/aspirate/dispense/purge sequences

• programmable dispense height

• programmable aspirate height

• programmable parameters for up to 10 plate types

• sweep mode for effective aspiration of flat-bottomed wells

• waste bottle with overflow sensing

• variable columns option

• all fluid-carrying tubes are external for convenient cleaning/replacing

• personal computer link for assay backup/storage Operation

Modes of Operation Manual/Automatic Microplate Types Flat, U and V-shaped wells; up to10 plate

types can be stored in memory 3 pre-programmed plate parameters

Aspirate/Dispense Head 8-way or 16-way floating head Programming Liquid crystal menu-driven display accessed

by simple function keys Computer interface Computer control for assay backup/storage

program Aspirate Mode

Aspirate System 4 litre waste bottle with overflow sense, froth trap bottle and aerosol trap jar

Aspirate Modes Normal and sweep modes Residual Volume (after aspirating)

≤1µl – U or V-shaped wells or ≤5µl in flat-bottomed wells

Soak Time 1 – 999 seconds in 1 second steps


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Dispense Mode Dispense Volume Range 50 – 2500 µl per well Max Wash Volume 2500 µl per well Dispense Capacity 2 x 2 litre, 4 x 2 litre, 2 x 4 litre bottle sets Wash Time ≤30 (16 way); ≤60 (8 way) secs / plate Wash Solutions 1 – 2 Wash Solutions selectable under

program control (4 solutions as a factory-fitted option)

Purge/Prime/Clean Mode Purging, priming or cleaning with specified solution

Auto Clean 1 minute to 24 hour intervals Dispense Precision ±5% C.V. (0.9% NaCl w/v) Dispense Pressure 4.3 – 7psi (adjustable by an approved

service engineer) Differences in dispense volumes can occur when tubing sets are changed. If tubing sets are changed, the Washer must be re-calibrated by an approved service engineer. Electrical Characteristics

GA Serial Numbers GB Serial Numbers Mains Supply Voltage (preset at factory)

115V 60/50Hz 230V 50/60Hz

100V 50Hz 115v 60Hz 230V 50Hz 230V 60Hz

Pump Fuse Requirements (2 per instrument)

115V: T4.0A 230V: T2.0A

100/115V: T4.0A 230V: T2.0A

Transformer Primary Fuse Requirements

115V: T500mA Slow Blow 230V T250mA Slow Blow

110/115V: T500mA Slow Blow 230V: T250mA Slow Blow

Transformer Secondary Fuse Requirements (2 per instrument)

Not fitted 1.5A Fast Blow

Power Consumption 150W Mains Plug Fuse 5A Safety Class 1 apparatus

Physical/Environmental Characteristics

Dimensions 432 x 340 x 170mm Weight 6.5Kg Operating Conditions 10 – 40°C; 30 – 75%RH

MULTI-REAGENT WASHER INSTALLATION

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INSTALLATION

WARNING: THIS INSTALLATION PROCEDURE SHOULD BE CARRIED OUT BY SKILLED ELECTRONIC TECHNICIANS. DO NOT apply power to the system until the installation procedure has been completed.

2.1 Unpacking The washer is packed to provide maximum protection during shipment. Remove the units from the carton carefully and check for any damage which may have occurred in transit. Report shipping damage to the carrier immediately. Check the contents against Figure 2.

Figure 2 Packing contents Note: To facilitate identification of individual components, tubing assemblies are not shown in detail.

1 Washer unit 8 Mains power cable 2 Bottle holder 9 QC document 3 Operating manual 10 8 way and 16 way head 4 Cleaning wires 11 Waste bottle and froth trap bottle 5 Tubing set 12 Dispense (wash solution) bottles 6 Plate carrier 13 Allen key 7 Spare filter 14 Aerosol trap jar


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2.2 Mains supply voltages CAUTION! Ensure that the voltages and fuse ratings on the rear panel of the unit correspond to the local mains supply. Serious damage may be caused if the unit is connected to the wrong power supply. Voltage settings are preset at the time of manufacture. It is not possible for users to change voltage settings on the washer. If the voltage specified on the instrument does not correspond to the local supply contact the nearest approved service centre. IMPORTANT: Fuses should only be replaced by a qualified service engineer.


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MULTI-REAGENT WASHER (MRW) Training Checklist

The MRW Training Checklist is provided as a guide to ensure that the critical functions of Installation, Operation and Maintenance have been covered during the training session.

• Installation Bottle and tubing assembly

Vacuum connection Pressure connection Pressure regulator Foam trap jar and filter Pinch valves

Electrical connections Power connection ON / OFF switch Float switch

Waste bottle full RS232 port EPROM access panel

Wash (dispense) head Posts (locked / unlocked) 8 – way 16 – way

Plate carrier

• Instrument panel Programming keys Main menu

Self-diagnostic tests Start – recall previously stored wash protocol Program – define new wash protocol Set-up – set washer parameters

Continued on next page


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• Washer set-up Autoclean

Time interval Fluid Volume

Bottom (double) aspirate Wash head Plate set-up

Up to 10 types of plates Sweep function Head height settings

Aspirate Dispense

Number of valves (wash solutions) Reset RAM 2 or 4 litre dispense bottles Shipping

• Operation Manual

MOVE / ASPIRATE / DISPENSE / PURGE / FINISH Program

Program name or number Plate type Variable columns Wash again Options menu

MOVE / PURGE / SOAK / END OF PROGRAM Loop menu

ASPIRATE / DISPENSE / PURGE / SOAK / END OF LOOP Edit

• Maintenance Flushing with water Cleaning

Spills Wash head Drip tray

• Review Operating Manual table of contents


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2.3 Rear panel connections The washer rear panel comprises:

Figure 3 Washer rear panel

• mains inlet socket

• mains power on/off switch: I – on, 0 – off • RS232 cable socket

• float switch socket

• vacuum and pressure connections

• pressure regulator CAUTION: Ensure that pressure and vacuum tubes are applied to the correct connections during installation.


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2.4 Site installation The unit should be sited on a firm level surface to ensure smooth movement of the microplate carrier when the system is operational. The bottle holders may be conveniently positioned behind the unit. Wash solutions are dispensed under pressure supplied from the pressure outlet next to the pressure regulator on the rear of the washer unit. Waste fluid is aspirated by vacuum supplied from the adjacent vacuum inlet. The froth trap bottle and condensing jar prevent the possibility of froth entering the vacuum system. It is important to use tubing of the correct dimensions to avoid inaccuracies in dispense volumes. Note: The pressure regulator has been factory set and calibrated and should not be adjusted. Set up the MRW components as follows: 1. Place the plate carrier and aspirate/dispense head into position. 2. Stand the aspirate and dispense bottles in their bottle racks in a convenient position

adjacent to the instrument. 3. Check that the aerosol trap jar is hooked onto the aspirate bottle rack. 4. Connect the pressure and vacuum tubes as detailed in section 2.5.


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2.5 Tubing connections The pressure and vacuum tubing system is shown in Figure 5 for the 2-valve MRW, and Figure 6 for the 4-valve MRW. 1. Connect the pressure tube (P) from the dispense bottle tube assembly T-piece or +-

piece to the PRESSURE bulk head connector on the back of the washer. 2. Connect the 2mm dispense tubes (2 or 4 tubes depending on 2 or 4-valve MRW) to

the aspirate/dispense head. At a convenient position along their length, clip the tubes firmly into the pinch valve slots – see Figure 4. The tube for fluid A passes through the front pinch valve.

The tubes and pinch valves for the different fluid channels are colour coded: Channel A: Red Channel B: Blue Channel C: Yellow Channel D: Black

Note: For programs 1 – 5 described in Appendix A, Fluid A is the clean fluid and Fluid B is the wash fluid.

3. Connect the waste fluid tube from the waste bottle to the aspirate/dispense head. 4. Connect the vacuum tube (V) to the VACUUM bulk head connector on the back of the

washer. The vacuum line passes via the aerosol trap and the froth trap bottle to the waste bottle. An optional hydrophobic filter can be fitted between the aerosol trap and the instrument vacuum connector.

4. Connect the overflow sensor (grey lead) from the aspirate bottle to the FLOAT SWITCH socket at the back of the washer.

Figure 4 washer front view


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Figure 5 2-valve MRW pressure and vacuum connections

Figure 6 4-valve MRW pressure and vacuum connections


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2.6 Switching on 1. Ensure the plate carrier is in position. 2. Fill the wash bottles with wash solutions. 3. CAUTION: Note the voltage and fuse specifications on the rear panel of the unit

before proceeding and check that they correspond with the local mains voltage. Plug in the mains power cable.

4. Switch the washer on.

MULTI-REAGENT WASHER OPERATING INSTRUCTIONS

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OPERATING INSTRUCTIONS Each time the washer is switched on diagnostic system tests are performed, then the main menu level is accessible. The range of options available at each menu level can be seen by scrolling up or down using the ∧∧∧∧ ∨∨∨∨ keys. Functions are selected by pressing the ENT key. The STOP key can be pressed at any time to halt an operation and revert to the main menu. Note: This manual is based on a washer unit with two valves. If four valves have been installed at the factory, the programme automatically adjusts fluid options and commands to allow access to all four valves A, B, C and D. THE MAIN MENU Scroll through the washer main menu using the ∧∧∧∧ ∨∨∨∨ keys to display the four operational modes:

• START - this mode allows the user to recall and run test sequences which have been defined in the PROGRAM mode (below) and are stored in the washer memory.

• PROGRAM - the operator will use this mode to construct complex and repetitive test sequences which can be stored in the washer memory and activated in the START mode (above).

• MANUAL - using this mode the user assumes direct control over the instrument and can manually move the plate to the desired column, aspirate, dispense fluids or purge the system.

• SETUP - this mode permits the operator to preset some of the system attributes and make fine adjustments to the head height in the aspirate and dispense positions for up to 10 plate types.


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3.1 SETUP Selecting the SETUP mode enables the user to specify certain attributes for the washer and these can be chosen by scrolling up or down using the cursor keys:

• AUTO CLEAN

• BOTTOM ASPIRATE

• DISPENSE HEAD

• PLATE SETUP

• NO. OF VALVES

• RESET RAM

• BOTTLE SET

• SHIPPING

• MAIN MENU Selection is made by pressing ENT when the chosen option is displayed. Pressing the STOP key at any time will return the system to the main menu. CANCEL ENTRY is one of the options available at each stage and it can be selected to return to the previous menu level. AUTO CLEAN The washer can be programmed to clean the system automatically at intervals from 1 minute to 24 hours. This facility is useful to keep the tubes clear when certain fluids are being used (e.g. fluids which form crystals and block the dispense heads). Press ENT to select AUTO CLEAN. The display will respond with:

AUTO CLEAN : ON Press ENT again and the time currently set between cleaning operations will be displayed in hours and minutes:

INTERVAL : 01:00 Press ENT to confirm the existing setting or adjust the interval in 1 minute steps by pressing the ∧ ∨ keys. Press ENT when the interval displayed is correct.


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A choice of fluid A or B will be offered for cleaning. Scroll to choose either:

CLEAN FLUID : A or

CLEAN FLUID : B Press ENT to select the fluid, then specify the amount of fluid in microlitres. An amount from 50µl to 2500µl can be specified in 5µl steps:

PURGE FLUID : A

VOLUME : 1000 µl The displayed amount is selected by pressing the ENT key. Next choose the purge fluid then specify the amount in microlitres. An amount from 50µl to 2500µl can be specified in 5µl steps:

PURGE FLUID : A

VOLUME : 1000 µl The displayed amount is selected by pressing the ENT key and the system returns to the set-up menu. A 5 second bleep alarm will sound prior to the execution of the programmed auto clean operation. BOTTOM ASPIRATE The user is able to choose between a normal aspirate operation and a double aspirate action in which the head aspirates in the normal way then returns to the base of the well and performs a second and shorter aspirate cycle. This type of aspirate action is provided for critical applications where users require greater confidence that complete aspiration has occurred. Note: The secondary aspirate cycle will increase the aspirate time. Users should ensure that the longer aspirate time will be acceptable in time-critical situations. Selecting BOTTOM ASPIRATE displays the current aspirate operation:

BOTTOM ASP : NO Press ENT to confirm the choice or use the cursor keys to scroll to the alternative

BOTTOM ASP : YES The chosen aspirate action will remain valid for subsequent aspirate operations until the alternative is entered.


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DISPENSE HEAD The washer is able to accommodate dispense/aspirate heads with either 8 channels or 16 channels. At the dispense head option:

DISPENSE HEAD Press ENT to display the currently specified head type, e.g:

HEAD : 16 WAY Press ENT to confirm the existing setting or scroll to choose the alternative head type:

HEAD : 8 WAY PLATE SETUP The PLATE SETUP option enables the user to preset and store plate parameters for up to 10 plate types. Plates are numbered P0 to P9, plates P0 to P2 having default settings for flat, U and V-bottomed plates respectively. Selecting PLATE SETUP displays the current well shape for plate number P0, e.g:

P0 : F Scroll to a different plate type if required. Press the ENT key to confirm the selection. The plate can be named at the flashing cursor. Up to 12 letters, numbers and symbols can be accessed by scrolling. Press ENT to accept each character as required. When the name is complete press ENT again. If no name is required press ENT twice. The system moves on to offer the sweep mode:

SWEEP : ON Press ENT to select the sweep operation for plate type number P0, or scroll to choose sweep OFF. Next the system offers the aspirate head height position and the plate carrier moves across so that the head is located over the first two columns of the microplate. The system aspirates and dispenses continuously during a dispense cycle, and draws off any fluid above the tips of the aspirate tubes. 25 aspirate and 25 dispense positions are available enabling the user to perform fine adjustment of the head for close control of the meniscus profile at the top of the wells during top washing operations. The positions are numbered 0 – 24, 0 being the lowest head height and 24 the highest.


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The aspirate height currently specified for plate number P0 is displayed, e.g.

ASPIRATE P0 : 5 Press ENT to show the head height at the displayed value or scroll to a new value then press ENT. The head will position itself to show the specified height. New values can be entered until the user is satisfied that the aspirate head height is correct. Press ENT to confirm the final position. This value will be used in subsequent aspirate operations for plate type P0 until a new value is assigned. When the aspirate head height position has been recorded, the dispense height is set in a similar way:

DISPENSE P0 : 22 The PLATE SETUP operation can be repeated for up to 10 plates. The specified plate bottom shapes, sweep operation requirement and head height values will be stored and will be used in subsequent operations for each numbered plate until new values are assigned.

Default Plate Types P0 P1 P2 Name : F U V SWEEP : ON OFF OFF ASPIRATE HEIGHT : 5 0 0 DISPENSE HEIGHT : 22 20 20

NO. OF VALVES Selecting NO. OF VALVES allows the user to specify the number of valves (i.e. wash solutions) installed on the washer. The number of installed valves is normally set at manufacture and the washer will be correctly configured at the factory. When set correctly the programme automatically adjusts all fluid options and commands to allow access to all the installed valves. If the washer temporary memory (RAM) is corrupted or reset for any reason it may be necessary to specify the number of valves that correspond to the amount installed. The program displays the number of valves currently specified:

VALVES : 2 Press ENT to confirm the number displayed or enter a new value via the cursor keys. The system records any changes when ENT is pressed.


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RESET RAM If the RAM is reset, it will have the effect of clearing all user-programmed tests from the washer memory. However, the factory-installed wash tests and default plate types will be available again, even if they had previously been overwritten by user-programmed tests. Under normal operating circumstances it should not be necessary to reset the RAM. BOTTLE SET The washer can be operated with 2 litre or 4 litre reagent dispense bottles. At the bottle set option:

BOTTLE SET press ENT to display the currently specified bottle type, e.g:

2 LITRE BOTTLES Press ENT to confirm the existing setting or scroll to the alternative bottle type:

4 LITRE BOTTLES and press ENT for this selection. Note: The waste bottle size is not specified. SHIPPING If the instrument is to be transported, the following shipping procedure is advisable. Press ENT at the following display:

SHIPPING When the head and carrier are locked into position, the following message indicates that the instrument power supply can be switched off and the mains cable disconnected.

SWITCH OFF... The instrument can be switched on again in the normal way.


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3.2 MANUAL Selecting MANUAL mode enables the user to assume direct control of washer operations. Note: While MANUAL mode is in force the pump and vacuum unit operate continuously. The instrument will aspirate while the vacuum unit is in operation. Selecting:

FINISH or pressing the STOP key will terminate MANUAL mode. In MANUAL mode the following selections are available:

• MOVE

• ASPIRATE

• DISPENSE

• PURGE

• FINISH MOVE When MANUAL mode is selected the plate carrier will move into position so that the dispense head is over column 1 (and column 2 if a 16-way head is fitted). Select the MOVE option by pressing ENT and the display will show:

COLUMN : 1 The user can now select the desired column number from 1 to 12 by scrolling with the cursor keys. When ENT is pressed the plate will move to the selected column and the display returns to MOVE. If a 16-way head is fitted the head will also be positioned over the adjacent column. If the wrong column has been selected the procedure can be repeated. Note: Before attempting aspirate or dispense operations ensure that the waste bottles have sufficient free space to accept aspirated fluids and that the overflow sense lead is securely plugged into the rear of the machine – failure to do so will cause the following error message to appear on screen:

WASTE FULL Aspirating and dispensing are prevented when this message is displayed.


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ASPIRATE Selecting the ASPIRATE operation executes one aspirate cycle in the current plate position. Aspirate action will depend on the aspirate type specified in the SETUP mode (see BOTTOM ASPIRATE and PLATE SETUP). DISPENSE Selecting the DISPENSE operation allows the operator to manually dispense a controlled quantity of the designated fluid. For dispensing and purging operations the washer selects fluid by actuating one of the pinch valves on the washer top plate and releasing the specified fluid. The display prompts the user to choose to dispense:

FLUID : A or scroll to

FLUID : B Press ENT to confirm the choice. The volume of fluid to be dispensed will be displayed in microlitres:

VOLUME : 500 µl Any volume between 50 and 2500 microlitres, in 5 microlitre steps, may be specified. Press ENT to select the amount. The washer will immediately begin the dispense cycle. CAUTION: At certain settings of the head heights in the dispense position (e.g. if the head is set well above the plate top), dispensing large volumes of fluid can cause overflowing to occur. Serious damage to the instrument may occur if fluid is allowed to penetrate the case. Take care not to allow the plate to overflow.


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PURGE Selecting the PURGE option allows the tubes and head to be purged of impurities for critical applications. It is recommended that the clean line be purged before the first wash of each program session. Select PURGE. The display prompts the user to choose the purging fluid:


FLUID : B Press ENT to confirm the choice. The volume of fluid to be purged will be displayed in microlitres, e.g:

VOLUME : 1000 µl Any volume between 50 and 2500 microlitres, in 5 microlitre steps, may be specified. Press ENT to initiate the purging cycle. The plate is moved to the washer home position, fluid is pumped through the system into the plate carrier drip tray and the plate is returned to its previous position. FINISH Pressing FINISH will exit MANUAL mode. FINISH returns the plate to the washer home position and the display to the MANUAL option of the main menu.


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3.3 PROGRAM PROGRAM mode allows the user to compile, edit and store named test sequences to perform complex and repetitive tasks automatically. There are two menu levels within the program mode: The program options menu

• MOVE

• PURGE

• SOAK

• END OF PROGRAM

The program loop functions menu

• ASPIRATE

• DISPENSE

• PURGE

• SOAK

• END OF LOOP A test sequence can consist of several loops which will be performed in succession. ASSIGNING A NAME OR NUMBER TO A TEST SEQUENCE When PROGRAM mode is selected, the first test sequence is offered:

1 : WASH 1 Press ENT to access this test or scroll up and down to access a different test. When an existing test is chosen, the display will ask whether the test is to be deleted or edited. Select:

DELETE : YES to overwrite the test

DELETE : NO to edit the test. When a new test is chosen, the cursor will flash the letter A in the first character position:

6 : A


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Program names can be up to 12 characters in length and consist of numbers 0 – 9, uppercase letters, and the symbols — * $ / + % : and the blank space character (represented by _ on the flashing display). Scroll up or down with the cursor keys to display the chosen character and press ENT. The flashing cursor will move forward to the next character position. When the final character has been selected, press ENT again to indicate that the name is complete. To delete an incorrect character, select the backspace symbol in the adjacent character position and press ENT. If the backspace symbol is selected in the first character position, the display will no longer show the flashing cursor and ENT must be pressed to re-select the chosen test. PLATE TYPE The display proceeds to ask which plate type will be used.

P0 : F Scroll up or down to choose a plate type from P0 to P9 and press ENT. Plate types P0 to P2 have default settings for flat, U and V-bottomed plates respectively. See section 3.1 SETUP mode PLATE SETUP for plate programming information. VARIABLE COLUMNS OPTION This feature allows the user to vary the number of strips to be washed at program run time without reprogramming the number of columns.

VARIABLE : NO If VARIABLE : NO is selected the program will be executed as defined. If

VARIABLE : YES or

VARIABLE : TIMED is selected the variable columns option will be enabled. When the program is run the system will prompt the user to enter the number of strips to be washed. The program offers the choice of terminating the wash cycle after the last strip is washed or continuing the cycle for the programmed number of columns when the required number of strips has been washed. The program pauses for the wash time at each column position where washing would normally have occurred. WASH AGAIN OPTION The WASH AGAIN option is useful if a number of plates will be washed when the program is run. If WASH AGAIN : YES is selected, the pump will not switch off after each plate is washed.


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PROGRAM OPTIONS MENU

• MOVE allows the operator to select the range of well columns over which a series of operations are to be performed. A loop of operations can be programmed in this mode.

• PURGE flushes the tubes and head with the selected fluid to reduce the likelihood of contamination in the system.

• SOAK allows the operator to specify the length of time between operations (e.g. between dispensing and aspirating).

• END OF PROGRAM terminates a test sequence. CREATING A PROGRAM LOOP Each program will consist of at least one program loop. A loop is a sequence of operations on a specified number of columns. A program may end after a single loop or it may consist of a series of loops to be performed in succession. MOVE Selecting the MOVE option informs the system that the user wishes to perform a cycle of operations over a range of wells. The system issues a prompt to specify the range of columns under test beginning with the lower column number:

START COL. : 1 Scroll to the required column start number between 1 and 12 and press ENT. The system will respond with a prompt to enter the upper column number. Enter a number equal to (for single column tests) or greater than (for multiple column tests) the previous entry. E.g:

END COLUMN : 12 Washer functions may now be combined into cycles of operations to be executed repetitively. The number of times a cycle of operations is to be repeated is programmed by the user:

CYCLES : 1 Enter a number between 1 and 99.


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The user can now scroll through the loop functions menu: • ASPIRATE

• DISPENSE

• PURGE

• SOAK

• END OF LOOP

Note : 1. The home position for the loop functions menu is indicated by

the following display:

ASPIRATE 2. Each function selected will be repeated for the range of wells

specified at START and END COLUMN and the number of times specified in CYCLES above.

3. Selecting STOP at any time during programming will cancel the

programming session and return to the main program menu.

ASPIRATE To program an ASPIRATE option press ENT at:

ASPIRATE and the display will show:

ASPIRATE : YES Press ENT to select the aspirate option. The display will revert to show ASPIRATE. This is because the ASPIRATE display is the home position of the loop functions menu. Use the cursor keys to scroll up or down for the other available functions within the loop.


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DISPENSE To program a DISPENSE operation, press ENT at the:

DISPENSE display. A controlled quantity of designated fluid can be specified to be dispensed. During program operation the washer will select the fluid by operating one of the two pinch valves on the washer top plate. The display prompts the user to choose to dispense:


FLUID : B Press ENT to record the choice. The volume of fluid to be dispensed will be displayed in microlitres.

VOLUME : 500 µl Any volume between 50 and 2500 microlitres, in 5 microlitre steps, may be specified. Press ENT to select the amount. Next, the user must specify the number of times the dispense operation is to be performed:

TIMES : 1 The specified dispense operation can be repeated up to 99 times. Scroll to the required number of times and press ENT. The display immediately returns to show ASPIRATE which is the home position in the loop functions menu.


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PURGE Press ENT at the PURGE display to select PURGE. The display prompts the user to choose the purging fluid:


FLUID : B Press ENT to record the choice. The volume of fluid to be purged will be displayed in microlitres:

VOLUME : 500 µl Any volume between 50 and 2500 microlitres, in 5 microlitre steps, may be specified. Press ENT to record the purging cycle. If a PURGE operation is recorded, during the program the plate is moved to the washer home position, fluid is pumped through the system into the plate carrier drip tray and the plate is returned to its previous position. The display immediately returns to show ASPIRATE – the home position in the program functions menu. SOAK The SOAK option allows the user to specify a soak time, i.e. the time for which the system will pause between operations.

SOAK : 5 Secs. The time is specified in seconds: scroll up or down to choose a number between 1 and 999 and press ENT. The display immediately returns to show ASPIRATE – the user can again scroll through the loop functions menu. END OF LOOP The cycle of operations may be terminated by selecting END OF LOOP. The display will then return to the MOVE option of the program options menu.


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PROGRAM OPTIONS After a loop of operations has been recorded, the program options menu is displayed. The user has the option of creating another loop over specific wells, specifying a soak time for the whole plate or a system purge, or terminating the test sequence.

• MOVE

• PURGE

• SOAK

• END OF PROGRAM Selecting MOVE will allow another loop of operations to be specified. Selecting PURGE or SOAK outside a loop will cause the operation to be performed once only. Selecting END OF PROGRAM will terminate the test sequence and save the test to non-volatile memory.


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PROGRAM EDITING Previously entered programs can be inspected and modified if required by adding or deleting steps or altering the parameters of existing steps. Selecting STOP at any time during inspecting and editing will terminate the editing session without recording any changes and return to the main program menu. TO INSPECT AN EXISTING PROGRAM 1. Select the PROGRAM option at the MAIN MENU. 2. Using the cursor keys scroll up or down to access the required test e.g:

1:EXAMPLE 1 Press ENT to confirm the test. The display will offer:

DELETE : YES to overwrite the test or:

DELETE : NO to edit the test. Select DELETE : NO. Press ENT twice to confirm the name which is displayed and access the first step of the program. 2. The operator may now scroll through the sequence to review the program via the

display, pressing ENT to confirm each stage:

• the plate type

• variable option

• wash again option

• columns chosen and number of cycles which were specified in the MOVE option of the loop functions program, e.g:

DO 1–12 X 1 indicates that columns 1-12 have been selected for one cycle. 4. The ASPIRATE, DISPENSE, SOAK and PURGE options will be displayed in the

order in which they have been programmed to operate. Press ENT at each display to confirm the instruction.


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TO MODIFY AN EXISTING PROGRAM 1. Select the PROGRAM option at the MAIN MENU. 2. Using the cursor keys scroll up or down to access the required test e.g:

1:EXAMPLE 1 Press ENT. The display will ask whether the existing test is to be deleted or edited. To edit the test, scroll to:

DELETE : NO 3. Press ENT; the flashing cursor will appear at the end of the name. To change the

name of the test scroll to the backspace key and press ENT to delete as many characters as required, then enter new characters as described in section 3.3 – Assigning a name or number to a test sequence.

4. The operator may now scroll through the sequence to review the program via the

display and perform modifications. 5. Press ENT to confirm the plate type or scroll and enter to make a change. 6. The display indicates the variable option currently in force. Press ENT to confirm or

scroll to a different option and press ENT to record a change. 7. The display indicates whether the wash again option is selected. Press ENT to

confirm or scroll to a different option and press ENT to record a change. 8. Next the display shows the columns chosen and number of cycles which were

specified in the MOVE option of the loop functions program e.g:

DO 1–12 X 1 Press ENT to confirm. To record a change scroll through the following and select EDIT:

• EDIT

• INSERT

• DELETE New numbers may be entered for:

START COL. : 1 and

END COLUMN : 12 and the number of cycles can be altered as required.


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9. Select INSERT to insert a step or a sequence of steps prior to the previously displayed step in the sequence. Enter the additional operations as for a new program – see 3.3 Creating a program loop.

EXAMPLE : Consider a loop of tests to: ASPIRATE / DISPENSE / SOAK / ASPIRATE To add a system PURGE between the first ASPIRATE and DISPENSE operations:

• scroll through the loop till the DISPENSE operation is displayed

• select INSERT

• select PURGE and enter its parameters The PURGE operation will be added to the loop and the display will return to the DISPENSE step.

10. If a new step or sequence of steps is inserted before the:

DO 1–12 X 1 display, it will create a new loop to the program and END OF LOOP must be selected to end the INSERT mode. The display will return to:

DO 1–12 X 1 Press ENT to inspect and modify the remainder of the test sequence. 11. The display will indicate the ASPIRATE, DISPENSE, SOAK and PURGE instructions

which have been recorded, e.g:

DISP 175 µl A indicates that 175 µl of fluid A are to be dispensed. The user may confirm this by pressing ENT or scroll to:

• EDIT / INSERT / DELETE 12. Select INSERT to precede the step with another step. 13. Select EDIT to modify the indicated step. For steps with several components the EDIT

mode displays each step component to be confirmed or revised.


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EXAMPLE : To modify a PURGE cycle The current PURGE status is displayed:

PURGE 100 µl A Scroll to EDIT, and press ENT. The first component of the PURGE cycle is displayed:

FLUID A Select ENT to confirm the fluid type or scroll to FLUID B and press ENT. The display then shows the specified volume which may also be altered by scrolling to the required amount, then pressing ENT.

14. Select DELETE to delete the function which is displayed. Scroll to display DELETE

and press ENT. The program records the deletion and the display will immediately show the next step in the sequence.

Note: When modifying program loops the END OF LOOP message will be observed

at the end of a cycle of tests. Deleting END OF LOOP will cause the system to attempt to include any following steps in the loop.

When END OF PROGRAM is reached, the system display returns to the main menu:

MRW : PROGRAM Users are advised to inspect the modified test by scrolling through the sequence, pressing ENT to confirm each stage in order to check that changes to programs have produced the expected test sequences.


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3.4 START Precompiled test programs may be run by selecting the START mode:

MRW : START The display shows test number 1:

1:EXAMPLE 1 Press ENT to run this test sequence. If the variable columns option was enabled in PROGRAM mode the system prompts the user for the number of strips to be washed:

STRIPS : 12 Scroll to a number between 1 and 12 and press ENT. Note: If a 16-way head is installed only even numbers can be accepted. After the number of strips has been entered the test will be executed. If the number of strips to be washed is less than the number of columns specified in the MOVE command and if VARIABLE : TIMED has been selected, when the required number of strips has been washed the program pauses for the wash time at each column position where washing would normally have occurred. When a test is run the pump is activated immediately and the display shows:

WASHING... The system pauses to enable the vacuum/pressure to reach its operating level. During operation waste fluid will be aspirated into the first 4 litre bottle. Any froth will collect in the second bottle and turn back into liquid. The label on this bottle shows MAX LEVEL and when this point is reached, the bottle should be emptied. The aerosol trap allows any remaining moisture to condense and drop onto the sponge. When the aspirate bottle is full, the float switch will switch off the MRW and the display will show:

WASTE FULL If the WASH AGAIN option was enabled, when the test is finished the pump will remain on and the test can be selected again for the next plate or a different test can be chosen. Press STOP to terminate the wash session. Note: Tests may be aborted at any time by pressing the STOP key.

MULTI-REAGENT WASHER COMPUTER CONTROL

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COMPUTER CONTROL This section contains information on how to interface the MRW to an external personal computer. This should only be attempted by a computer operator who is well versed in computer communication protocol and peripheral handling. Note: Information on the Assay Storage/Backup Program available for linking the MRW with an IBM-compatible personal computer for the safe backup and extended storage capacity of user-defined test programs can be found in Appendix C. The computer interface is as specified by EIA RS232C interface standard for a data terminal device.

Figure 7 RS 232C pin assignments

MULTI-REAGENT WASHER COMPUTER CONTROL

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COMMAND SEQUENCE

Upload Computer MRW U Ack/Nak data ETX checksum Ack/Nak ETX Ack/Nak

Note: Several blocks of data may be sent of up to 100 characters in each. The final ETX on its own signifies end of data. The checksum is a four character hexadecimal number representing an integer value. This is the sum of all the ASCII characters.

Download Computer MRW D Ack/Nak data ETX checksum Ack/Nak

Etx = 03 Hex = 3 Decimal Ack = 06 Hex = 6 Decimal Nak = 15 Hex = 21 Decimal Some simple software to perform this is available.

MULTI-REAGENT WASHER MAINTENANCE

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MAINTENANCE 5.1 Routine maintenance

WARNING : ELECTRIC SHOCK HAZARD There are no user-serviceable components within this instrument. THE INSTRUMENT MUST NOT BE OPERATED WITH THE COVERS REMOVED - POTENTIALLY LETHAL VOLTAGES ARE CONTAINED WITHIN. If a fault should occur with your instrument, contact the nearest approved service centre.

CAUTION: Always disconnect the mains electrical supply from the system before attempting any cleaning procedures. See the Section SAFETY INFORMATION. The washer is constructed from high quality materials. However, spilt saline solutions, solvents, acids or bases should be removed immediately. Exterior surfaces should be cleaned with a mixture of water and mild detergent. The washer will require little maintenance apart from regularly checking tube connections, flushing through or replacing the tubes and bottle filter and cleaning the heads and drip tray.

• Tube and bottle connections should be regularly inspected for tightness to prevent vacuum and/or pressure leaks. Tubing should also be checked to make sure it is not kinked, restricted or damaged as this may lead to dispense problems.

• The system should be purged frequently to prevent the aspirate and dispense tubes from becoming blocked with residues from evaporated fluids.

CAUTION: The dispense/aspirate head must not be autoclaved. The aspirate and dispense tubes beneath the head body may be cleared of obstruction with the tube cleaning wires provided. To clear the dispense tubes remove the head from its mounting, remove the slotted screws from both ends of the head and carefully insert the cleaning wires; any residues from the tubes may be conveniently flushed out and the screws replaced. To clear the aspirate tubes remove the silicone rubber head cover with a small flat blade. The tubes can be cleared with the cleaning wires and the head easily swabbed out with cotton swabs. Push the head cover carefully back into place.


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Tube replacement Important: When replacing the waste tubes, ensure that the tube ends do not extend further than 1 centimetre beyond the holes in the bottle top. Should it become necessary to replace the tubes or bottles, the following components should be used. Using tubing with dimensions significantly different from those detailed below may lead to inaccuracies in the volumes of fluid dispensed. PVC tubing 3/8" outside diameter 1/4" inside diameter Large tubing: General purpose silicone rubber: 7.2mm outside diameter 4mm inside diameter Small tubing: High strength silicone compound: 3.2mm outside diameter 2.0mm inside diameter WARNING: To prevent possible injury or damage, use only the thick-walled bottles of the type provided as reservoirs. These bottles are designed to withstand the high vacuums and pressures in the system. Wash/waste bottles: high-density polyethylene with polypropylene caps 2 or 4 litre reagent and waste bottles Head adjustment If it is found that the head tips do not fit comfortably into the microplate wells, the head position on the mounts can be adjusted forwards or backwards. A 1.5ÿmm hexagonal allen key is provided to adjust the grub screws found at each end of the head. By screwing in the screw at one end and correspondingly unscrewing the screw at the opposite end, the head position can be adjusted for best fit. 5.2 Fuse replacement If the washer fails to function, first check the power cable is firmly connected. Fuses should only be replaced by a qualified service engineer. The live and neutral pump fuses are contained on the printed circuit board inside the instrument. Unscrew and remove the instrument cover. Carefully prise off the plastic fuse casings to access the fuses. Contact an approved service centre if the transformer fuse requires replacement. CAUTION: To avoid the risk of fire the fuses should only be replaced with the correct type and rating. See Electrical Characteristics in the SPECIFICATIONS section of this manual.


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5.3 Troubleshooting and Error messages Error Messages There are 5 error messages which if persistent will require attention from approved service personnel. DISPLAY CHECKSUM ERROR EFFECT fatal BATTERIES FLAT tests lost CORRUPT RAM tests possibly corrupted PLATE ERROR CAUSE motor overload Up/down error Dispensing Problems If the dispense volume appears to be incorrect:

• Check for leaks in the dispense bottle

• Check for damaged, kinked or restricted tubing.

MULTI-REAGENT WASHER

40

MULTI-REAGENT WASHER Appendix A: Default Assay and Plate Formats

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Appendix A: Default Assay and Plate Formats Default Assay Formats On first power-up, five default wash programs are initialised. These programs can be overwritten if required. If the RAM is reset, the pre-programmed tests will be available even if they were previously deleted.

Test Number: 1 Test Name: WASH 1 Plate Type: U Variable: TIMED Wash Again: NO PURGE: 1000µl Fluid B Do Columns 1 – 12 1 Times { DISPENSE 500µl of Fluid B } Do Columns 1 – 12 1 Time { ASPIRATE } PURGE: 1000µl Fluid A.

Tests numbers 2 to 5 follow the same format except that each test has one more wash cycle in it. Thus test number 5 has 5 cycles. Default Plate Types On first power up, three plate formats are initialised. These plate formats can be overwritten as required.

P0 P1 P2 Name : F U V SWEEP : ON OFF OFF ASPIRATE HEIGHT : 5 0 0 DISPENSE HEIGHT : 22 20 20

MULTI-REAGENT WASHER Appendix B: Assay Backup/Storage Program

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Appendix B: Assay Backup/Storage Program The MRW Assay Backup/Storage Program has been devised to link the Multi-Reagent Washer with an IBM-compatible personal computer for two purposes:

• For the safe backup of test programs. In the event of MRW memory failure (caused by flat batteries or corrupt RAM, for example), programs can be easily retrieved from the backup disk.

• To extend the storage capacity for user-defined test programs The on-board memory of the MRW can store up to 40 test programs (a maximum of 25 kbytes) in non-volatile memory. User programs can now be transferred from the MRW to a PC and stored in batches of up to 40 programs, thereby freeing the MRW memory for a further batch of up to 40 programs.

The MRW Test Storage/Backup Program comprises one file – MRW.EXE. It is supplied on a 360kb 5.25" or 1.4Mb 3.5" diskette and can be run on any IBM- compatible PC. The Program may be operated directly from the diskette, or transferred to hard disk following normal DOS copy commands (see relevant DOS reference manual). Connecting the MRW to the PC Ensure that the MRW and PC are switched off and disconnected from the mains supply before making the connection. Using the standard cable supplied with the MRW, connect the MRW RS232C port to the PC COM1or COM2 (serial communication) port. Switch the MRW on. To run the Assay Backup/Storage Program, the MRW must always be at the Main Menu level (START, SETUP, PROGRAM or MANUAL), for example:

MRW : START The following program operating instructions assume that the user is familiar with normal PC operating routines. Commands are typed in using the keyboard and selected by pressing the Enter or Return key.


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Operating instructions Insert the disk into the PC disk drive and type MRW. The PC prompts the user to type in the communication port in use:

M.R.W. on COM1 or COM2 (1 or 2): ? Type in 1 or 2 and press Enter. The program menu is then displayed:

MRW Assay Backup

1 – MRW to Disk 2 – Disk to MRW Q – Quit program

• MRW TO Disk – saves tests from MRW to disk

• Disk TO MRW – returns previously saved tests from disk to MRW. Select 1, 2 or Q (to quit the program) as required. Saving tests from the MRW to PC Select 1 to save tests from the MRW to disk. The display prompts the user to specify the disk and directory where the test is to be stored:

Input DISK DRIVE to receive transfer (A, B, C, etc.) ? Input DIRECTORY to receive transfer (\DATA etc. RETURN for none) Input FILENAME.EXTN (default = .DAT) to receive transfer

A question mark and flashing cursor guide the user through the prompts. For example, to save the MRW test programs to A:\EXAMPLE.DAT

• At Input DISK DRIVE to receive transfer (A, B, C etc.) ? type A No colon (:) is required as it is inserted by the software.

• At Input DIRECTORY to receive transfer (\DATA etc.RETURN for none)? type \MRW The backslash (\) is required as it is NOT inserted by the software.

• At Input FILENAME.EXTN (default = .DAT) to receive transfer type EXAMPLE or EXAMPLE.DAT If the user does not type in an extension the default .DAT extension will be used. If a different extension is required it must be specified.

The system summarises the transfer instruction:

Transfer will be made to A:\EXAMPLE.DAT Accept (A), Change (C) or Exit to menu (E) ?

If the display is incorrect type C, and retype the information.


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When the display is correct type A. If the file already exists, a warning appears:

File already exists: Program will continue Do you want to overwrite this file ? (Y/N)

Select N to specify a new filename and destination. When the instructions are correct the system will transfer all the test programs. The screen displays the progress of the transfer in 500byte blocks:

Receiving data from MRW… Transferring data to disk… Amount of data transferred

0 5 10 15 20 25 30 kbytes = 500 bytes

When the transfer is complete the operation is summarised on screen Press Enter to return to the Assay Backup menu and quit the program. Transferring previously saved tests from PC to MRW Select 2 at the Assay Backup menu. The display prompts the user to specify the disk and directory where the test is to be stored, and the filename:

Input DISK DRIVE of PC source file (A, B, C, etc.) ? Input DIRECTORY of PC source file (\DATA etc. RETURN for none) Do you want a listing of the above drive and directory (Y/N) Input file extension for listing (default extn. = .DAT) Input FILENAME.EXTN of PC source file

A question mark and flashing cursor guide the user through the prompts. For example, to transfer test programs from A:\MRW\EXAMPLE.DAT

• At Input DISK DRIVE of PC source file (A, B, C etc.) ? type A No colon (:) is required as it is inserted by the software.

• At Input DIRECTORY of PS source file (\DATA etc.RETURN for none)? type \MRW The backslash (\) is required as it is NOT inserted by the software.

• At Do you want a listing of the above drive and directory (Y/N) type N to enter a known filename. The cursor moves to the last prompt. type Y to list filenames of the relevant extension.

• At Input file extension for listing (default extn. = .DAT) type .DAT The system lists all files of that extension in the specified directory. This allows the user to choose from the files available.

• At Input FILENAME.EXTN of PC source file type EXAMPLE.DAT


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The system summarises the instructions:

Transfer will be made from: A:\MRW\EXAMPLE.DAT Accept (A), Change (C) or Exit to menu (E) ?

If the display is incorrect type C, and retype the information; if it is correct, type A. A percentage bar displays the progress of the transfer:

Transmitting data to MRW… Reading disk information… – Bytes of data to transfer Transferring data…

0% 50% 100% > <

The completed transfer will be summarised:

– bytes successfully transferred to MRW Press RETURN to continue

Press Enter to return to the Assay Backup menu and quit the program. Error/check Messages The program issues messages designed to help the user locate the cause in the event of program failure. If the error persists contact an approved service centre, (addresses are listed in the rear of this manual). Communications errors "Device Timeout" "MRW not responding to PC" "Bad file number" "MRW interrupted during operation"

• Check that the MRW is switched on and is at the highest menu level.

• Check the cable connection between the MRW and PC and check that the correct COM port has been specified.

• If the communication error message persists after the fault is rectified the user should quit the program and re-enter to clear the message.

Disk and file errors "File not found" "Bad filename" "Disk not ready" "File already open" "Too many files" "Disk media error" "File already exists"

• Disk and file errors usually relate to the state of the disk (e.g. damaged or full), overwrite safeguards and invalid directories. The on-screen message will indicate the nature of the problem and possible solution.

MULTI-REAGENT WASHER Appendix C: Accessories

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Appendix C: Accessories

Bottle Rack (2x2 litre) 200-2646-00 Bottle Rack (2x4 litre) 200-0743-00 Head, 8-way 020-0063-00 Head, 16-way 020-0064-00 Bottle, 2-litre Nalgene 029-0527-00 Bottle, 4-litre Nalgene 029-0274-00 1 x 2 litre dispense bottle set 000-8560-05 2 x 4 litre dispense bottle set 000-8560-06 1 x 4 litre dispense bottle set 000-8560-07 Tubing, Silicone 2mm ID x 3.2mmOD 816-4005-00 Tubing, Silicone 4mm ID x 7.2mm OD 816-4006-00 Tubing, Silicone 2mm ID x 5mm OD 816-4010-00 Hydrophobic Filter 402-2003-00 Cleaning Wire, 0.018" 352-1018-00 Cleaning Wire, 0.040" 352-1040-00 Power Cord (Europe) 575-1006-00 Power Cord (U.K.) 575-1007-00 Power Cord (U.S.A.) 575-1008-00 Fuse T2A 20mm (220-240V) 519-1200-02 Fuse T4A 20mm (110-120V) 519-1400-02 RS232C cable 040-0061-00 Assay Backup/Storage Program 000-8230-00

MULTI-REAGENT WASHER CONTACT US

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CONTACT US Please Contact your local sales office for accessories, technical assistance or information on product servicing. DYNEX Technologies, Inc. 14340 Sullyfield Circle Chantilly, VA 20151 USA Tel: 703-631-7800 Toll Free: 800-288-2354 Fax: 703-803-1441 E-mail: [email protected]

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