Thermoresponsive polymers for biomedical applications
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Transcript of Thermoresponsive polymers for biomedical applications
Course:advance polymers
Dilshad bajwa
Roll#11
Introduction “Smart polymer” materials that have the ability to
respond to external stimuli, represent one of the most
exciting and immerging class of materials
They are cheaper and more easily handled than metals
or ceramics.
Stimulus-responsive or ‘smart’
polymers
Smart polymers and hydrogels undergo fast,
reversible changes in microstructure from a
hydrophilic to a hydrophobic state with change in
(pH, temperature, ionic strength) etc.
Temperature-responsive polymers
Respond to temperature changes
Types of thermo responsive polymer:
TRP which shows LCSTTRP which shows UCSTe.g poly(acrylonitrile-co-butadiene)
Temperature responsive polymer
The first established LCST is 32oC for poly(N-isopropylacrylamide) (PNIPAAm) in water solutionAt this temp:
•Undergoes a sharp coil-globule transition•Changes from hydrophilic state to hydrophobic state as temperature is increases•In aqueous solutions, it is soluble below LCST and less soluble above it
Gene Therapy:
Gene therapy aims at the treatment of many genetic diseases .Delivery of therapeutic gene (DNA) into the cells that will replace, repair or regulate the defective gene.
Gene therapy aims at the treatment of many genetic diseases .Delivery of therapeutic gene (DNA) into the cells that will replace, repair or regulate the defective gene.
The main steps of gene delivery
using a cationic polymer:
(1) DNA complexation formation
(polyarginine polymer AND PNIPAAm
)above LCST
(2) Complex traversing through cell membrane to the cytoplasm at 37 °C
(3) DNA release into the cytoplasm below LCST
(4) DNA transfer into nucleus
Tissue Engineering
To regenerate or replace biological damaged or diseased tissue or generate replacement organs for a wide range of medical conditions such as heart diseases, diabetes, and spinal cord injury .
Thermoresponsive polymer is mixed at room temperature with cells and then injected into the body.Upon injection due to the temperature increase (to 37 °C) that is above the polymer’s LCST, the polymer forms a physical gel.
Drug delivery through Micelles
The thermoresponsive properties of terpolymers of PEGMA-b-(DMAEMA-co-MMA)
The formation of thermoresponsive micelles with sustained drug release in vitro
Cross Linked Micelles
The formation of micelles above the LCST of PEG-b-PPG-b-PEG triblock copolymersBy incorporating a methacrylate group onto each end of the block copolymers crosslinking.crosslinked layer slowed the release of drug by entrapment but allowed an increase in the release with increased temperature
Formation of nanocages from polymers of PEG (blue) PPG (red) and methacrylate groups (green)
Polymer bilayer film entraps particles and cells.
Conclusion:Thermoresponsive polymers and their uses in gene therapy ,genetic engineering .drug delivery etc. have a definite impact in the medical field.
THANKS FOR LISTENING