How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

Flow panel sizes have expanded dramatically in the last 15+ years

and continue to do so.

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

An antibody panel with more than 10-colors is no longer uncommon,

and with the adaptation of the more recent Brilliant dyes, 14+ colors are now very feasible on

many instruments.

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

With this increase in parameter detection per sample, the subsetting of

T cells in the literature has exploded.

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

For example, when considering one aspect of T cell biology, the naïve to memory differentiation post-antigen exposure, the field has transcended

from an era of two subsets (naïve and memory) into the era of Central

Memory, Transitional Memory, Effector Memory, Terminally Differentiated

Effector Memory, etc.

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

To find out more, read this article on T cell subsets and their functions (◄ read the full article here) in the

world of flow cytometry.

R E A D T H E F U L L A R T I C L E

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

W E P R O V I D E F L O W C Y T O M E T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

Of course, knowing about T cell subsets is only one of the many elements you need to

know about clinical flow cytometry!

Another is knowing when to use the proper flow cytometry analysis software!

R E A D T H E F U L L A R T I C L E

W E P R O V I D E F L O W C Y T O M E N T R Y T R A I N I N G T O A C A D E M I C S A N D

I N D U S T R Y P R O F E S S I O N A L S

A B O U T E X C Y T E C Y T O M E T R Y

Expert Cytometry, or ExCyte, was born out of the desire to make flow cytometry accessible to anyone interested in learning it. Flow Cytometry used to have an almost cultish existence, using battered notebooks to pass down knowledge. The problem was that this information generally never wnet beyond a particular institution. Learning was dependent on who you knew (or trial and error over the years), which slowed down progress. We formed ExCyte with the purpose of gathering people who are as passionate about learning successful flow cytometry as we are. Our goal is to make a lifetime of learning flow cytometry available to everyone. Our team has decades of experience teaching and managing flow labs and we collaborate closely with others like us to present a systematic, structured, and enjoyable program.

D I S C OV E R M O R E A B O U T E XC Y T E H E R E !

C O N N E C T W I T H E X C Y T E