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The World’s Leading Manufacturer of Peptide Synthesizers

®

■ Maximize flexibility■ 20 L solvent container■ Sensor deliveries■ Automated cleaning routines

■ 50 mL collection tube capacity■ 12 positions may alternatively be used as Single-ShotTM AA bottles ■ Dedicated TFA bottle position■ Customizable cleavage programs■ Adjustable start times

The Symphony X Peptide Synthesizer®

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■ Maximum Throughput & Production■ Fast, Efficient Couplings■ High Quality Crude Peptides■ Extremely Flexible ■ Automated Cleavage

■ 0.005 - 22.4 mmol scales ■ Up to 12 RV’s with pre-activation■ Up to 24 RV’s with in situ activation■ Adjustable nitrogen mixing ■ Independent operation ■ Top-down washing ■ Optional IR heating &/or UV-monitoring capability in RV position 1

Automated Cleavage

12 Independent Reaction Vessel Pairs

28 Amino Acid Positions

■ 10, 120 & 400 mL sizes available■ Single-ShotTM deliveries■ Pre-packed amino acid bottles available ■ Sensor deliveries■ Automated

8 Solvent Positions

Integrated Solvent Cabinet

■ Enclosed, ventilated & easily accessible■ Floor-standing model with wheels easily fits in your lab■ Chemical spill trays for spill containment■ Pull out drawer for convenient storage

■ Full color■ Resistant to all synthesis chemicals■ Flexible, easy-to-use software■ No external computer!

VGA 17” Touchscreen

High Quality PerformanceHigh Purity

Crude Peptides

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Unbeatable Throughput!

Deprotection Time: 4 min

Coupling Time: 5 min

Cycle Time: 23 min

Total Synthesis Time, 24 Peptides: 25 hours!

Deprotection Time: 2 min

Coupling Time: 5 min

Cycle Time: 21 min

Total Synthesis Time, 24 Peptides: 14 hours!

(H-DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA-OH)

Twenty-four 68-mers in 25 hours!Twenty-four 42-mers in 14 hours!

C-peptide (H-EAEDLQVGQVELGGGPGAGSLQPLALEGSLG-OH)

24 reaction vessels gives you 24 times the throughput of any serial peptide synthesizer! When it comes to custom peptide production, nothing can match the Symphony® X!

β-amyloid1-42

PurifiedPeptide

Crude Peptide

65-74ACP (H-VQAAIDYING-OH)

Chemokine SDF-1a

(H2N-KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK-OH)

PurifiedPeptide

Crude Peptide

■ Full color■ Resistant to all synthesis chemicals■ Flexible, easy-to-use software■ No external computer!

■ In situ activation can be accomplished by delivering amino acid to the reaction vessel first, followed by activator

Choose Between In Situ & Pre-activation

■ Top row reaction vessels may be used as pre-activation chambers for bottom row reaction vessels.

REACTION VESSEL

ACT

1.

2.

BOTTOM ROW REACTION

VESSEL

TOP ROW PREACTIVATION

CHAMBER

AA

ACT

AA

12 Synthesizers in One!Run different sequences, scales and protocols on multiple reactors all at the same time! Start, Stop or Pause reactors whenever you wish! It’s like having 12 synthesizers in one! Each reaction vessel pair can:

■ Run Any Program■ Run Any Scale■ Run Any Reagent ■ Run Any Reaction Time■ Start/Stop/Pause

regardless of what the other reaction vessels are doing!

Innovative Features

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PreactivationNow you can preactivate on multiple reactors in parallel. Perfect for sterically hindered amino acid additions! Optimize your DIC/HOBt chemistry on the Symphony® X and go straight to scale-up!

Robust, Reliable Construction

Other systems use off-the-shelf valves that leak, cross-contaminate, and quickly fail when exposed to harsh peptide synthesis chemicals. Our patented valve block system was specifically designed for peptide synthesis, so you get zero dead volumes, no cross-contamination, and worry-free performance that lasts for years!

Special Reagent AdditionsAt last! Add expensive special reagents to any reactor without priming or wasting a drop using Single-Shot™ deliveries! Save money with no manual intervention required! The Symphony® X comes with up to 40 amino acid positions to accommodate any chemistry!10 mL Single-Shot

Amino Acid Vial

Automated Cleavage*The Symphony® X features automated cleavage. Unlike some peptide synthesizers, all PTI synthesizers are built with robust TFA-resistant materials, so you can cleave for years on our instruments without worry. Not interested in cleavage? You can also use the Symphony® X collect positions as additional amino acid positions!

*Note: Fmoc chemistry only

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UV-Monitoring

Minimize trial-and-error when synthesizing difficult peptides using real-time UV-monitoring on the Symphony® X. What makes our UV-monitoring different? Other systems just monitor the waste stream. We monitor your reaction solution while it’s mixing – that’s why we’re the only ones who can control the deprotection reaction times, not just the number of repetitions!

Safe-Response FeatureKeep your reactions safe during unexpected interruptions. When your synthesis is interrupted for any reason, the Symphony® X will:

■ Drain active reaction vessels■ Wash resin with solvent of your choice■ Prevent side reactions and undesired byproducts

E-mail NotificationMonitor the status of your synthesis from your:

Computer Cell Phone

Choose to be notified:

■ At the beginning of each cycle■ When an error occurs■ At the end of a synthesis

Infrared (IR) Heating

■ Faster than microwaves■ Test the same protocol with and without heat in the same synthesis!■ Vortex mixing ensures no hot spots!■ Most stable heating system on the market today - doesn’t overshoot!■ Highly accurate temperature sensing for precise temperature control■ Minimize process variables during scale-up

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Flexible, Easy-To-Use Software

■ Automated cleaning routines■ Waste level detection■ Manual operations■ Customizable amino acid and solvent tables■ Calculations screens■ Synthesis record■ Good Laboratory Practice data recording■ Diagnostics and error reporting■ Flexible restart options for interrupted syntheses

Create, view or edit programs

Select a sequence(s) and assign a program to each

cycle

Load synthesis and monitor progress of all reactions

Other Software FeaturesAll With Easy

Touchscreen Programming

Specifications for PTI products and services on all pages in this publication are subject to change without notice. “PS3” is a trademark and “Overture,” “Symphony, ” “Prelude,” “Tribute” and “Sonata” are registered trademarks of Protein Technologies, Inc. PTI products are manufactured under U.S. Patent 5,203,368, other U.S. and international patents issued and pending. Copyright 2013 Protein Technologies, Inc. All rights reserved. DOC #9080106 Rev 01

Use PTI ReagentsAfter choosing the right synthesizer, nothing is more important to your synthesis success than the quality of the reagents used with your instrument. Use PTI brand amino acids to maximize the performance of your

automated peptide synthesizer. Our amino acids are quality screened and optimized for use on PTI instruments to help ensure the production of high quality peptides and the reliable operation of your instrument, and come in affordable pre-weighed bottles for your convenience as well as in bulk.

Accessories, Reagents & Service

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Accessories

■ 10 mL GlassReaction Vessel

Cat#: TPS-GRV10-1, 1 ea.Cat#: TPS-GRV10-10, 10

■ 45 mLReaction Vessel

Cat#: PPS-R45-030, 30Cat#: PPS-R45-090, 90

Cat#: PPS-R45-180, 180

■ 40 mL GlassReaction Vessel

Cat#: TPS-GRV40-1, 1 ea.Cat#: TPS-GRV40-10, 10

■ 10 mL Reaction Vessel

Cat#: PPS-R10-030, 30Cat#: PPS-R10-090, 90

Cat#: PPS-R10-180, 180

■ 10 mL Single-ShotTM

AA Bottle Cat#: AAR-SSI, 1 ea.

Cat#: AAR-SSX, 10

■ 50 mLCollect Vial

Cat#: CLV-050-030, 30

■ 120 mL AA Bottle

Cat#: SMP-VX-020, 20Cat#: SMP-VX-100, 100

■ 400 mL AA Bottle

Cat#: AAR-400I, 1 ea.Cat#: AAR-400X, 10

PTI Service and SupportIn addition to supplying superior instrumentation, PTI offers the finest in technical support and field service. Our service team specializes in one thing - supporting PTI synthesis instruments. From installation to preventative maintenance, our responsive field service group will make sure that you get the most out of your investment.

■ PTI offers varying levels of service agreements■ Please contact PTI for terms and pricing

■ 10 mL Frosted Glass Reaction

Vessel Cat#: TPS-FGRV10-1, 1 ea.Cat#: TPS-FGRV10-10, 10

■ 40 mL Frosted Glass Reaction

VesselCat#: TPS-FGRV40-1, 1 ea. Cat#: TPS-FGRV40-10, 10

Ordering Information:

Number of reaction vessels ............................... 24 (12 with pre-activation)Synthesis scale range ....................................... 0.005 mmol to 22.4 mmol (up to ~2 g of resin per RV)Reaction vessel volume .................................... Plastic, disposable - 10 mL and 45 mL Glass - 10 mL and 40 mLNumber of solvent positions/sizes .................... 1 primary solvent position, 20 L capacity 7 user-defined solvent positions, 0.5 L - 4 L capacitiesNumber of amino acid positions ....................... Up to 40 amino acid positions 10, 120 or 400 mL bottlesChemistries supported ..................................... Fmoc, t-Boc, organic, peptoid, combinatorial, othersActivation .......................................................... DIC/HOBt, HBTU, HATU, HCTU, TBTU, PyBOP, othersThroughput ....................................................... User definedFluid measurement technique .......................... Fixed loop measurement with optical sensorsFluid transfer method ........................................ Positive pressure with nitrogenAgitation method ............................................... Nitrogen bubbling &/or vortex mixing - adjustable and programmableCleavage ........................................................... Automatic, programmable (for Fmoc chemistry only)Waste container ................................................ 20 L container with overflow sensorControl interface ................................................ Proprietary Symphony® X control software Internal computer and touchscreen monitor includedReporting .......................................................... Real-time log of each instrument function, print to file, screen or printer. Power ................................................................ 115 V/60 Hz or 230 V/50 HzDimensions ........................................................ 45”W, 29”D, 66”H (114 cm W, 74 cm D, 168 cm H)Weight ............................................................... 550 lbs. (250 kg)Warranty ............................................................ One year, parts and laborOptional ............................................................... Infrared (IR) Heating (SYMX-IR-OPT) UV-Monitoring (SYMX-UV-OPT) E-mail/Text Message Status Reporting (SYMX-TXT-EML-RPT)

Catalog # DescriptionSYMX-110 Symphony® X Peptide Synthesizer, 115 VSYMX-230 Symphony® X Peptide Synthesizer, 230 V

Symphony X Specifications®

For more information: Email: info@ptipep.comWeb: www.ptipep.com

Main Office:4675 South Coach DriveTucson, AZ 85714, U.S.A.Phone: +1-520-629-9626Toll Free: 1-800-477-6834Fax: +1-520-629-9806